{"id":"https://openalex.org/W2017956221","doi":"https://doi.org/10.1021/bi00298a008","title":"Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase","display_name":"Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase","publication_year":1984,"publication_date":"1984-01-31","ids":{"openalex":"https://openalex.org/W2017956221","doi":"https://doi.org/10.1021/bi00298a008","mag":"2017956221","pmid":"https://pubmed.ncbi.nlm.nih.gov/6367818"},"language":"en","primary_location":{"id":"doi:10.1021/bi00298a008","doi":"https://doi.org/10.1021/bi00298a008","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00298a008","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026939827","display_name":"John G. Koland","orcid":"https://orcid.org/0000-0002-5028-4297","observed_orcids":["https://orcid.org/0000-0002-5028-4297"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John G. Koland","raw_affiliation_strings":["Cornell University","University of Illinois at Urbana-Champaign"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cornell University","institution_ids":["https://openalex.org/I205783295"]},{"raw_affiliation_string":"University of Illinois at Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Michael J. Miller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael J. Miller","raw_affiliation_strings":["University of Illinois at Urbana Champaign"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Illinois at Urbana Champaign","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008855880","display_name":"Robert B. Gennis","orcid":"https://orcid.org/0000-0002-3805-6945","observed_orcids":["https://orcid.org/0000-0002-3805-6945"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Robert B. Gennis","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":5.0092,"has_fulltext":false,"cited_by_count":104,"citation_normalized_percentile":{"value":0.96317448,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"23","issue":"3","first_page":"445","last_page":"453"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.4961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.4961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13012","display_name":"Coenzyme Q10 studies and effects","score":0.13729999959468842,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.13099999725818634,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pyruvate-oxidase","display_name":"pyruvate oxidase","score":0.9919999837875366},{"id":"https://openalex.org/keywords/ubiquinone-8","display_name":"ubiquinone-8","score":0.8019999861717224},{"id":"https://openalex.org/keywords/flavoprotein-dehydrogenases","display_name":"flavoprotein dehydrogenases","score":0.8840000033378601},{"id":"https://openalex.org/keywords/membrane-reconstitution","display_name":"membrane reconstitution","score":0.5789999961853027},{"id":"https://openalex.org/keywords/phospholipid-vesicles","display_name":"phospholipid vesicles","score":0.9300000071525574},{"id":"https://openalex.org/keywords/electron-transport","display_name":"electron transport","score":0.6190000176429749},{"id":"https://openalex.org/keywords/membrane-potential","display_name":"membrane potential","score":0.9629999995231628},{"id":"https://openalex.org/keywords/uncouplers","display_name":"uncouplers","score":0.5189999938011169},{"id":"https://openalex.org/keywords/valinomycin","display_name":"valinomycin","score":0.6970000267028809},{"id":"https://openalex.org/keywords/fccp","display_name":"FCCP","score":0.8500000238418579}],"concepts":[{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.7047127485275269},{"id":"https://openalex.org/C2778407487","wikidata":"https://www.wikidata.org/wiki/Q14565201","display_name":"Altmetrics","level":2,"score":0.6713874340057373},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.5655661225318909},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5233825445175171},{"id":"https://openalex.org/C3020377403","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Respiratory chain","level":3,"score":0.4498042166233063},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.3813379108905792},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3697654604911804},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.3143727779388428},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.3122829496860504},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3073332607746124},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14151400327682495}],"mesh":[{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D003573","descriptor_name":"Cytochrome b Group","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003580","descriptor_name":"Cytochromes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004579","descriptor_name":"Electron Transport","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008051","descriptor_name":"Lipid Bilayers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008564","descriptor_name":"Membrane Potentials","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011771","descriptor_name":"Pyruvate Oxidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014451","descriptor_name":"Ubiquinone","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D045222","descriptor_name":"Electron Transport Chain Complex Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00298a008","doi":"https://doi.org/10.1021/bi00298a008","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00298a008","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:6367818","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6367818","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.5299999713897705,"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":51,"referenced_works":["https://openalex.org/W45732365","https://openalex.org/W101549160","https://openalex.org/W126869179","https://openalex.org/W988916465","https://openalex.org/W1066204754","https://openalex.org/W1443021588","https://openalex.org/W1484479305","https://openalex.org/W1485377960","https://openalex.org/W1506420820","https://openalex.org/W1520666478","https://openalex.org/W1522246139","https://openalex.org/W1530273007","https://openalex.org/W1542508542","https://openalex.org/W1549322844","https://openalex.org/W1564432135","https://openalex.org/W1565395667","https://openalex.org/W1572432795","https://openalex.org/W1579005896","https://openalex.org/W1587442005","https://openalex.org/W1605262192","https://openalex.org/W1658192173","https://openalex.org/W1775749144","https://openalex.org/W1843350440","https://openalex.org/W1963045321","https://openalex.org/W1964716852","https://openalex.org/W1978145631","https://openalex.org/W1979238462","https://openalex.org/W1995688498","https://openalex.org/W1997402636","https://openalex.org/W2000711880","https://openalex.org/W2001042770","https://openalex.org/W2002506570","https://openalex.org/W2022414255","https://openalex.org/W2024512883","https://openalex.org/W2026389952","https://openalex.org/W2027228112","https://openalex.org/W2033965578","https://openalex.org/W2037955199","https://openalex.org/W2039467756","https://openalex.org/W2043220092","https://openalex.org/W2052503065","https://openalex.org/W2062498369","https://openalex.org/W2063441382","https://openalex.org/W2066790202","https://openalex.org/W2069902567","https://openalex.org/W2070116290","https://openalex.org/W2109606373","https://openalex.org/W2111725375","https://openalex.org/W2145239230","https://openalex.org/W2147761506","https://openalex.org/W2316513465"],"related_works":["https://openalex.org/W1594549221","https://openalex.org/W2140062494","https://openalex.org/W1566871748","https://openalex.org/W1592869261","https://openalex.org/W1415018605","https://openalex.org/W2593109602","https://openalex.org/W1564432135","https://openalex.org/W2407542583","https://openalex.org/W1997345778","https://openalex.org/W2034430539"],"abstract_inverted_index":{"Pyruvate":[0],"oxidase":[1,36,154,197,203],"is":[2,38,45,87,96,103,129,142,162],"a":[3,41,118,200,207,220],"flavoprotein":[4,68,95,167],"dehydrogenase":[5],"located":[6],"on":[7],"the":[8,12,20,29,34,67,70,83,94,114,132,146,152,156,159,166,174,193,211,225],"inner":[9],"surface":[10],"of":[11,31,48,82,90,121,148,158],"Escherichia":[13],"coli":[14,22,74,100,213,227],"cytoplasmic":[15],"membrane":[16],"and":[17,40,69,137,191,204],"coupled":[18],"to":[19,111,131],"E.":[21,73,99,212,226],"aerobic":[23,214],"respiratory":[24,43,215,228],"chain.":[25,216,229],"In":[26],"this":[27,64,188],"paper,":[28],"role":[30],"quinones":[32],"in":[33,57,63,113,155,187,210],"pyruvate":[35,110],"system":[37,86,116],"investigated,":[39],"minimal":[42],"chain":[44],"described":[46],"consisting":[47],"only":[49],"two":[50],"pure":[51],"proteins":[52],"plus":[53],"ubiquinone":[54,171,185],"8":[55,172,179,186,202],"incorporated":[56],"phospholipid":[58,175],"vesicles.":[59],"The":[60,79],"enzymes":[61],"used":[62],"reconstitution":[65],"are":[66],"recently":[71],"purified":[72],"cytochrome":[75,194],"d":[76,195],"terminal":[77,153,196],"oxidase.":[78],"catalytic":[80],"velocity":[81],"reconstituted":[84,97],"liposome":[85,115],"about":[88],"30%":[89],"that":[91,106,164],"observed":[92],"when":[93],"with":[98],"membranes.":[101],"It":[102,161],"also":[104,143],"shown":[105],"electron":[107],"transport":[108],"from":[109],"oxygen":[112],"generates":[117],"transmembrane":[119],"potential":[120,141],"at":[122],"least":[123],"180":[124],"mV":[125],"(negative":[126],"inside),":[127],"which":[128],"sensitive":[130],"uncouplers":[133],"carbonyl":[134],"cyanide":[135],"p-(tri-chloromethoxy)phenylhydrazone":[136],"valinomycin.":[138],"A":[139],"trans-membrane":[140],"generated":[144],"by":[145,151],"oxidation":[147],"ubiquinol":[149,201],"1":[150],"absence":[157],"flavoprotein.":[160],"concluded":[163],"(1)":[165],"can":[168],"directly":[169],"reduce":[170],"within":[173],"bilayer,":[176],"(2)":[177],"menaquinone":[178],"will":[180],"not":[181],"effectively":[182],"substitute":[183],"for":[184,224],"electron-transfer":[189],"chain,":[190],"(3)":[192],"functions":[198],"as":[199,206],"serves":[205],"\"coupling":[208],"site\"":[209],"These":[217],"investigations":[218],"suggest":[219],"relatively":[221],"simple":[222],"organization":[223]},"counts_by_year":[{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}