{"id":"https://openalex.org/W2017936731","doi":"https://doi.org/10.1016/0014-5793(86)81004-3","title":"Reduction of EGF receptor synthesis by antisense RNA vectors","display_name":"Reduction of EGF receptor synthesis by antisense RNA vectors","publication_year":1986,"publication_date":"1986-10-06","ids":{"openalex":"https://openalex.org/W2017936731","doi":"https://doi.org/10.1016/0014-5793(86)81004-3","mag":"2017936731","pmid":"https://pubmed.ncbi.nlm.nih.gov/2428666"},"language":"en","primary_location":{"id":"doi:10.1016/0014-5793(86)81004-3","doi":"https://doi.org/10.1016/0014-5793(86)81004-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/0014-5793(86)81004-3","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2886%2981004-3","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2886%2981004-3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5008808306","display_name":"John Hunts","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John Hunts","raw_affiliation_strings":["Dept of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA","Department of Molecular & Cellular Biology, University of Arizona, Tucson, AZ, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"Department of Molecular & Cellular Biology, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012702025","display_name":"Glenn T Merlino","orcid":"https://orcid.org/0000-0001-8610-0616","observed_orcids":["https://orcid.org/0000-0001-8610-0616"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Glenn Merlino","raw_affiliation_strings":["Laboratory of Molecular Biology, NCI, Bethesda, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, NCI, Bethesda, MD, USA","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056573254","display_name":"Ira H Pastan","orcid":"https://orcid.org/0000-0002-9223-0270","observed_orcids":["https://orcid.org/0000-0002-9223-0270"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ira Pastan","raw_affiliation_strings":["Laboratory of Molecular Biology, NCI, Bethesda, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, NCI, Bethesda, MD, USA","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108377872","display_name":"Nobuyoshi Shimizu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]},{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Nobuyoshi Shimizu","raw_affiliation_strings":["Dept of Molecular Biology, Keio University School of Medicine, Tokyo, Japan","Dept of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept of Molecular Biology, Keio University School of Medicine, Tokyo, Japan","institution_ids":["https://openalex.org/I203951103"]},{"raw_affiliation_string":"Dept of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":0.8579,"has_fulltext":true,"cited_by_count":10,"citation_normalized_percentile":{"value":0.75541605,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"206","issue":"2","first_page":"319","last_page":"322"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10755","display_name":"HER2/EGFR in Cancer Research","score":0.9477999806404114,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10755","display_name":"HER2/EGFR in Cancer Research","score":0.9477999806404114,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.033399999141693115,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/egf-receptor","display_name":"EGF receptor","score":0.9490000009536743},{"id":"https://openalex.org/keywords/antisense-rna","display_name":"antisense RNA","score":0.9610000252723694},{"id":"https://openalex.org/keywords/squamous-cell-carcinoma","display_name":"squamous cell carcinoma","score":0.9279999732971191},{"id":"https://openalex.org/keywords/cdna-fragments","display_name":"cDNA fragments","score":0.7080000042915344},{"id":"https://openalex.org/keywords/gene-expression-inhibition","display_name":"gene expression inhibition","score":0.5820000171661377}],"concepts":[{"id":"https://openalex.org/C111335779","wikidata":"https://www.wikidata.org/wiki/Q3454686","display_name":"Reduction (mathematics)","level":2,"score":0.6351209878921509},{"id":"https://openalex.org/C202416437","wikidata":"https://www.wikidata.org/wiki/Q423832","display_name":"Antisense RNA","level":4,"score":0.6139490008354187},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5622044205665588},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.45372653007507324},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.41456758975982666},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3692600131034851},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28804194927215576},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2734561562538147},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10116621851921082},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.06800052523612976},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002294","descriptor_name":"Carcinoma, Squamous Cell","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004815","descriptor_name":"Epidermal Growth Factor","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066246","descriptor_name":"ErbB Receptors","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D066246","descriptor_name":"ErbB Receptors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0014-5793(86)81004-3","doi":"https://doi.org/10.1016/0014-5793(86)81004-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/0014-5793(86)81004-3","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2886%2981004-3","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:2428666","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2428666","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/0014-5793(86)81004-3","doi":"https://doi.org/10.1016/0014-5793(86)81004-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/0014-5793(86)81004-3","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2886%2981004-3","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9074000120162964,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2017936731.pdf","grobid_xml":"https://content.openalex.org/works/W2017936731.grobid-xml"},"referenced_works_count":9,"referenced_works":["https://openalex.org/W1894695711","https://openalex.org/W1966648387","https://openalex.org/W1973071934","https://openalex.org/W2016568386","https://openalex.org/W2046954253","https://openalex.org/W2047512715","https://openalex.org/W2062059447","https://openalex.org/W2095081971","https://openalex.org/W2129414194"],"related_works":["https://openalex.org/W2089439493","https://openalex.org/W1553385403","https://openalex.org/W2409810384","https://openalex.org/W3145423085","https://openalex.org/W2375075806","https://openalex.org/W2351381529","https://openalex.org/W2354495957","https://openalex.org/W2374051741","https://openalex.org/W2424097155","https://openalex.org/W240560602"],"abstract_inverted_index":{"An":[0],"association":[1],"has":[2],"been":[3],"observed":[4],"between":[5],"squamous":[6],"cell":[7],"carcinomas":[8],"and":[9,55],"elevated":[10],"EGF":[11,32,47],"receptor":[12,48],"levels.":[13],"The":[14,34,67],"approach":[15],"taken":[16],"here":[17],"to":[18,23],"analyze":[19],"this":[20],"problem":[21],"was":[22,38,73],"construct":[24],"vectors":[25],"which":[26,42],"express":[27],"antisense":[28,65],"RNA":[29],"for":[30,60],"the":[31,71,74],"receptor.":[33],"plasmid":[35],"vector":[36],"used":[37],"derived":[39],"from":[40],"pSV2-neo,":[41],"is":[43],"readily":[44],"selectable.":[45],"Three":[46],"cDNA":[49],"fragments,":[50],"a":[51,53,56],"5'-,":[52],"middle":[54],"3'-region":[57],"were":[58],"tested":[59],"differences":[61],"in":[62],"efficiency":[63],"as":[64],"RNA.":[66],"results":[68],"suggest":[69],"that":[70],"5'-fragment":[72],"most":[75],"efficient.":[76]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}