{"id":"https://openalex.org/W2017782609","doi":"https://doi.org/10.1111/j.1574-6968.2000.tb09134.x","title":"A novel episomal shuttle vector for transformation of Cryptococcus neoformans with the ccdB gene as a positive selection marker in bacteria","display_name":"A novel episomal shuttle vector for transformation of Cryptococcus neoformans with the ccdB gene as a positive selection marker in bacteria","publication_year":2000,"publication_date":"2000-06-01","ids":{"openalex":"https://openalex.org/W2017782609","doi":"https://doi.org/10.1111/j.1574-6968.2000.tb09134.x","mag":"2017782609","pmid":"https://pubmed.ncbi.nlm.nih.gov/10828398"},"language":"en","primary_location":{"id":"doi:10.1111/j.1574-6968.2000.tb09134.x","doi":"https://doi.org/10.1111/j.1574-6968.2000.tb09134.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1574-6968.2000.tb09134.x","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070124338","display_name":"Philippe Mondon","orcid":"https://orcid.org/0000-0002-3538-2200","observed_orcids":["https://orcid.org/0000-0002-3538-2200"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Philippe Mondon","raw_affiliation_strings":["National Institute of Allergy and Infectious Diseases, N.I.H., Bethesda, MD 20892, USA","Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Allergy and Infectious Diseases, N.I.H., Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I4210134534"]},{"raw_affiliation_string":"Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025807967","display_name":"Yun C. Chang","orcid":"https://orcid.org/0000-0002-8706-3615","observed_orcids":["https://orcid.org/0000-0002-8706-3615"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yun C Chang","raw_affiliation_strings":["Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ashok Varma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ashok Varma","raw_affiliation_strings":["Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103112617","display_name":"Kyung Joo Kwon-Chung","orcid":"https://orcid.org/0000-0003-3383-1428","observed_orcids":["https://orcid.org/0000-0003-3383-1428"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Kyung J Kwon-Chung","raw_affiliation_strings":["Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Microbiology Section, LCI, National Institute of Allergy and Infectious Diseases, N.I.H., Bldg. 10, 11C304, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5103112617"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"],"apc_list":{"value":2500,"currency":"GBP","value_usd":3297},"apc_paid":null,"fwci":1.432,"has_fulltext":true,"cited_by_count":31,"citation_normalized_percentile":{"value":0.80104277,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"187","issue":"1","first_page":"41","last_page":"45"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9229000210762024,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9229000210762024,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11037","display_name":"Fungal Infections and Studies","score":0.03240000084042549,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.028699999675154686,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cryptococcus-neoformans","display_name":"Cryptococcus neoformans","score":0.9829999804496765},{"id":"https://openalex.org/keywords/plasmid-cloning","display_name":"plasmid cloning","score":0.6449999809265137},{"id":"https://openalex.org/keywords/transformation-frequency","display_name":"transformation frequency","score":0.5860000252723694},{"id":"https://openalex.org/keywords/gene-expression-regulation","display_name":"gene expression regulation","score":0.5220000147819519}],"concepts":[{"id":"https://openalex.org/C2777893358","wikidata":"https://www.wikidata.org/wiki/Q131924","display_name":"Cryptococcus neoformans","level":2,"score":0.9538882374763489},{"id":"https://openalex.org/C145640855","wikidata":"https://www.wikidata.org/wiki/Q7505713","display_name":"Shuttle vector","level":5,"score":0.9081240892410278},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8367354869842529},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.8056917786598206},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.7614613771438599},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.5933078527450562},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5174575448036194},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5007669925689697},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4753912687301636},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.4595467448234558},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.44532907009124756},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.41152721643447876},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001427","descriptor_name":"Bacterial Toxins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003455","descriptor_name":"Cryptococcus neoformans","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004271","descriptor_name":"DNA, Fungal","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012641","descriptor_name":"Selection, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014170","descriptor_name":"Transformation, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015139","descriptor_name":"Blotting, Southern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1111/j.1574-6968.2000.tb09134.x","doi":"https://doi.org/10.1111/j.1574-6968.2000.tb09134.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1574-6968.2000.tb09134.x","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},{"id":"doi:10.1016/s0378-1097(00)00168-3","doi":"https://doi.org/10.1016/s0378-1097(00)00168-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0378-1097(00)00168-3","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},{"id":"pmid:10828398","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10828398","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS microbiology letters","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W1872803163","https://openalex.org/W1957562398","https://openalex.org/W1965656523","https://openalex.org/W1979046954","https://openalex.org/W1990777342","https://openalex.org/W2000804360","https://openalex.org/W2069477714","https://openalex.org/W2090490520","https://openalex.org/W2096855445","https://openalex.org/W2111580125","https://openalex.org/W2124590513","https://openalex.org/W2159527831","https://openalex.org/W2165159573"],"related_works":["https://openalex.org/W2090490520","https://openalex.org/W2159527831","https://openalex.org/W2380726435","https://openalex.org/W2124590513","https://openalex.org/W2392763791","https://openalex.org/W2159134252","https://openalex.org/W1972842851","https://openalex.org/W1872803163","https://openalex.org/W4404536304","https://openalex.org/W2020314765"],"abstract_inverted_index":{"We":[0],"report":[1],"the":[2,17,34,41,65,74,93],"engineering":[3],"of":[4,64,81,95],"a":[5],"new":[6],"shuttle":[7],"vector":[8,42,50],"featuring":[9],"its":[10],"episomal":[11,38],"maintenance":[12,39],"in":[13,26,46,70,97],"Cryptococcus":[14],"neoformans":[15,32,76],"and":[16,33,55,91],"lethal":[18],"Escherichia":[19],"coli":[20,72],"ccdB":[21],"gene":[22],"for":[23,89],"positive":[24],"selection":[25],"bacteria.":[27],"Telomere-like":[28],"sequences":[29],"from":[30,73],"C.":[31,47,75,98],"STAB":[35],"fragment":[36],"confer":[37],"to":[40,60],"(pPM8)":[43],"upon":[44],"transformation":[45,53],"neoformans.":[48,99],"The":[49,67],"generated":[51],"high":[52],"frequencies":[54],"each":[56],"transformant":[57],"was":[58],"estimated":[59],"harbor":[61],"thirty":[62],"copies":[63],"plasmid.":[66],"plasmids":[68],"recovered":[69],"E.":[71],"transformants":[77],"showed":[78],"no":[79],"evidence":[80],"rearrangement.":[82],"This":[83],"construct":[84],"will":[85],"be":[86],"very":[87],"useful":[88],"cloning":[90],"studying":[92],"regulation":[94],"genes":[96]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":4}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
