{"id":"https://openalex.org/W2017741617","doi":"https://doi.org/10.1104/pp.59.5.842","title":"Pyruvate Dehydrogenase Complex from Higher Plant Mitochondria and Proplastids","display_name":"Pyruvate Dehydrogenase Complex from Higher Plant Mitochondria and Proplastids","publication_year":1977,"publication_date":"1977-05-01","ids":{"openalex":"https://openalex.org/W2017741617","doi":"https://doi.org/10.1104/pp.59.5.842","mag":"2017741617","pmid":"https://pubmed.ncbi.nlm.nih.gov/16659953","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/543307"},"language":"en","primary_location":{"id":"doi:10.1104/pp.59.5.842","doi":"https://doi.org/10.1104/pp.59.5.842","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.59.5.842","pdf_url":"https://academic.oup.com/plphys/article-pdf/59/5/842/35580287/plphys_v59_5_842.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/plphys/article-pdf/59/5/842/35580287/plphys_v59_5_842.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103396562","display_name":"E. Ellen Reid","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204722609","display_name":"Queen's University","ror":"https://ror.org/02y72wh86","country_code":"CA","type":"education","lineage":["https://openalex.org/I204722609"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"E. Ellen Reid","raw_affiliation_strings":["Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6","institution_ids":["https://openalex.org/I204722609"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Paul Thompson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204722609","display_name":"Queen's University","ror":"https://ror.org/02y72wh86","country_code":"CA","type":"education","lineage":["https://openalex.org/I204722609"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Paul Thompson","raw_affiliation_strings":["Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6","institution_ids":["https://openalex.org/I204722609"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111676212","display_name":"C. Richard Lyttle","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C. Richard Lyttle","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5108347193","display_name":"David T. Dennis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204722609","display_name":"Queen's University","ror":"https://ror.org/02y72wh86","country_code":"CA","type":"education","lineage":["https://openalex.org/I204722609"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"David T. Dennis","raw_affiliation_strings":["Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6","institution_ids":["https://openalex.org/I204722609"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":2.4293,"has_fulltext":true,"cited_by_count":118,"citation_normalized_percentile":{"value":0.89100186,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"59","issue":"5","first_page":"842","last_page":"848"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12915","display_name":"Biochemical Acid Research Studies","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12915","display_name":"Biochemical Acid Research Studies","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pyruvate-dehydrogenase-complex","display_name":"pyruvate dehydrogenase complex","score":1.0},{"id":"https://openalex.org/keywords/proplastids","display_name":"proplastids","score":0.8460000157356262},{"id":"https://openalex.org/keywords/plant-mitochondria","display_name":"plant mitochondria","score":0.9079999923706055},{"id":"https://openalex.org/keywords/pea","display_name":"pea","score":0.7089999914169312},{"id":"https://openalex.org/keywords/castor-bean-endosperm","display_name":"castor bean endosperm","score":0.9089999794960022},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.652999997138977},{"id":"https://openalex.org/keywords/thiamine-pyrophosphate","display_name":"thiamine pyrophosphate","score":0.8519999980926514},{"id":"https://openalex.org/keywords/magnesium-requirement","display_name":"magnesium requirement","score":0.5640000104904175},{"id":"https://openalex.org/keywords/cysteine","display_name":"cysteine","score":0.796999990940094},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.8450000286102295},{"id":"https://openalex.org/keywords/subcellular-enzyme-localization","display_name":"enzyme subcellular localization","score":0.5270000100135803},{"id":"https://openalex.org/keywords/acetyl-coa-carboxylase","display_name":"acetyl-CoA carboxylase","score":0.8600000143051147},{"id":"https://openalex.org/keywords/succinate-dehydrogenase","display_name":"succinate dehydrogenase","score":0.9079999923706055},{"id":"https://openalex.org/keywords/triton-x-100-solubilization","display_name":"Triton X-100 solubilization","score":0.7630000114440918}],"concepts":[{"id":"https://openalex.org/C88099638","wikidata":"https://www.wikidata.org/wiki/Q412856","display_name":"Pyruvate dehydrogenase complex","level":3,"score":0.8517401218414307},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6842796802520752},{"id":"https://openalex.org/C101972281","wikidata":"https://www.wikidata.org/wiki/Q899965","display_name":"Pyruvate decarboxylation","level":4,"score":0.6824507713317871},{"id":"https://openalex.org/C151325109","wikidata":"https://www.wikidata.org/wiki/Q22327132","display_name":"Branched-chain alpha-keto acid dehydrogenase complex","level":4,"score":0.659572184085846},{"id":"https://openalex.org/C190098323","wikidata":"https://www.wikidata.org/wiki/Q14912712","display_name":"Pyruvate dehydrogenase phosphatase","level":4,"score":0.654029905796051},{"id":"https://openalex.org/C9506865","wikidata":"https://www.wikidata.org/wiki/Q6321025","display_name":"Pyruvate dehydrogenase kinase","level":4,"score":0.6259390711784363},{"id":"https://openalex.org/C126093377","wikidata":"https://www.wikidata.org/wiki/Q309518","display_name":"Oxoglutarate dehydrogenase complex","level":5,"score":0.6068122386932373},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5435556769371033},{"id":"https://openalex.org/C140137476","wikidata":"https://www.wikidata.org/wiki/Q21114968","display_name":"Pyruvate carboxylase","level":3,"score":0.5292792916297913},{"id":"https://openalex.org/C2780047943","wikidata":"https://www.wikidata.org/wiki/Q415003","display_name":"Thiamine pyrophosphate","level":4,"score":0.4919745624065399},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.4315011501312256},{"id":"https://openalex.org/C80097345","wikidata":"https://www.wikidata.org/wiki/Q419036","display_name":"Dihydrolipoyl transacetylase","level":5,"score":0.42465895414352417},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.3238760828971863},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.17194032669067383}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1104/pp.59.5.842","doi":"https://doi.org/10.1104/pp.59.5.842","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.59.5.842","pdf_url":"https://academic.oup.com/plphys/article-pdf/59/5/842/35580287/plphys_v59_5_842.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:543307","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/543307","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"},{"id":"pmid:16659953","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16659953","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1104/pp.59.5.842","doi":"https://doi.org/10.1104/pp.59.5.842","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.59.5.842","pdf_url":"https://academic.oup.com/plphys/article-pdf/59/5/842/35580287/plphys_v59_5_842.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2017741617.pdf","grobid_xml":"https://content.openalex.org/works/W2017741617.grobid-xml"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W132838376","https://openalex.org/W1490403537","https://openalex.org/W1507940589","https://openalex.org/W1516242195","https://openalex.org/W1586173121","https://openalex.org/W1775749144","https://openalex.org/W1856489983","https://openalex.org/W1864809073","https://openalex.org/W1940420259","https://openalex.org/W1971715529","https://openalex.org/W1971781312","https://openalex.org/W1974240746","https://openalex.org/W1975672120","https://openalex.org/W1984167610","https://openalex.org/W1990720750","https://openalex.org/W2020812722","https://openalex.org/W2025603513","https://openalex.org/W2033536855","https://openalex.org/W2063894433","https://openalex.org/W2076269026","https://openalex.org/W2331037034","https://openalex.org/W2376788889","https://openalex.org/W2395503246","https://openalex.org/W6600218635","https://openalex.org/W6602629387","https://openalex.org/W6609594587"],"related_works":["https://openalex.org/W2017243037","https://openalex.org/W2023179933","https://openalex.org/W2037958361","https://openalex.org/W1974240746","https://openalex.org/W2041806418","https://openalex.org/W2267841601","https://openalex.org/W1971781312","https://openalex.org/W4214859773","https://openalex.org/W2066763314","https://openalex.org/W1984042260"],"abstract_inverted_index":{"The":[0,21,39,66,159,190,199],"pyruvate":[1,131,148,176],"dehydrogenase":[2,94,132,136,142,149,177],"complex":[3,22,36,40,71,95,133,150,178,191,200],"from":[4,91],"pea":[5],"(Pisum":[6],"sativum":[7],"L.)":[8,88],"mitochondria":[9,116],"was":[10,72,97,214],"purified":[11],"23-fold":[12],"by":[13,120],"high":[14],"speed":[15],"centrifugation":[16,122],"and":[17,45,48,58,75,115,123,137,151,205],"glycerol":[18],"gradient":[19],"fractionation.":[20],"had":[23,192],"a":[24,31,167,193],"s(20,w)":[25],"of":[26,108,113,126,129,174,197],"47.5S":[27],"but":[28,211],"this":[29],"is":[30,37,41,180,185],"minimal":[32],"value":[33],"since":[34],"the":[35,70,103,109,124,127,130,147,157,175,188],"unstable.":[38],"specific":[42],"for":[43,63,69,208],"NAD(+)":[44],"pyruvate;":[46],"NADP(+)":[47],"other":[49],"keto":[50],"acids":[51],"give":[52],"no":[53],"reaction.":[54],"Mg(2+),":[55,203],"thiamine":[56,206,212],"pyrophosphate,":[57],"cysteine":[59],"are":[60],"also":[61],"required":[62,201],"maximal":[64,172,209],"activity.":[65],"pH":[67,195],"optimum":[68,196],"between":[73],"6.5":[74],"7.5.Continuous":[76],"sucrose":[77],"density":[78],"gradients":[79],"were":[80,143],"used":[81],"to":[82,99,134,140,163],"separate":[83],"castor":[84],"bean":[85],"(Ricinus":[86],"communis":[87],"endosperm":[89],"proplastids":[90,114],"mitochondria.":[92],"Pyruvate":[93],"activity":[96,173,179,210],"found":[98],"be":[100,118,164],"coincident":[101],"with":[102,145,166],"proplastid":[104,160],"peak":[105],"on":[106],"all":[107],"gradients.":[110],"Some":[111],"separation":[112],"could":[117],"achieved":[119],"differential":[121],"ratios":[125],"activities":[128],"succinic":[135,141],"acetyl-CoA":[138,152],"carboxylase":[139,153],"consistent":[144],"both":[146],"being":[154],"present":[155],"in":[156,187],"proplastid.":[158],"fraction":[161],"has":[162],"treated":[165],"detergent,":[168],"Triton":[169],"X-100,":[170],"before":[171],"expressed,":[181],"indicating":[182],"that":[183],"it":[184],"bound":[186],"organelle.":[189],"sharp":[194],"7.5.":[198],"added":[202],"cysteine,":[204],"pyrophosphate":[207,213],"inhibitory":[215],"at":[216],"higher":[217],"concentrations.":[218]},"counts_by_year":[{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}