{"id":"https://openalex.org/W2017721591","doi":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","title":"Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory","display_name":"Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory","publication_year":1999,"publication_date":"1999-02-20","ids":{"openalex":"https://openalex.org/W2017721591","doi":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","mag":"2017721591","pmid":"https://pubmed.ncbi.nlm.nih.gov/10099550"},"language":"en","primary_location":{"id":"doi:10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","doi":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026070090","display_name":"Maria I. Klapa","orcid":"https://orcid.org/0000-0002-2047-3185","observed_orcids":["https://orcid.org/0000-0002-2047-3185"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maria I. Klapa","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032307633","display_name":"Sung Min Park","orcid":"https://orcid.org/0000-0003-2653-9955","observed_orcids":["https://orcid.org/0000-0003-2653-9955"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sung M. Park","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042953895","display_name":"Anthony J. Sinskey","orcid":"https://orcid.org/0000-0002-1015-1270","observed_orcids":["https://orcid.org/0000-0002-1015-1270","https://orcid.org/0000-0001-9433-4324"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anthony J. Sinskey","raw_affiliation_strings":["Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009763658","display_name":"Gregory N Stephanopoulos","orcid":"https://orcid.org/0000-0001-6909-4568","observed_orcids":["https://orcid.org/0000-0001-6909-4568"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gregory Stephanopoulos","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5009763658"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":3.3656,"has_fulltext":false,"cited_by_count":86,"citation_normalized_percentile":{"value":0.93080757,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"62","issue":"4","first_page":"375","last_page":"391"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9115999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9115999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.07460000365972519,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/isotopomer-distribution","display_name":"isotopomer distribution","score":0.7900000214576721},{"id":"https://openalex.org/keywords/tricarboxylic-acid-cycle","display_name":"TCA cycle","score":0.8970000147819519},{"id":"https://openalex.org/keywords/metabolic-cycles","display_name":"metabolic cycles","score":0.7289999723434448},{"id":"https://openalex.org/keywords/metabolic-flux-analysis","display_name":"metabolic flux analysis","score":0.652999997138977},{"id":"https://openalex.org/keywords/13c-nmr-spectroscopy","display_name":"13C NMR","score":0.7910000085830688},{"id":"https://openalex.org/keywords/14c-labeled-substrates","display_name":"14C-labeled substrates","score":0.8009999990463257},{"id":"https://openalex.org/keywords/molecular-weight-distribution","display_name":"molecular weight distribution","score":0.6460000276565552},{"id":"https://openalex.org/keywords/label-distribution","display_name":"label distribution","score":0.5080000162124634},{"id":"https://openalex.org/keywords/mathematical-model","display_name":"mathematical model","score":0.5460000038146973}],"concepts":[{"id":"https://openalex.org/C127124265","wikidata":"https://www.wikidata.org/wiki/Q2100870","display_name":"Isotopomers","level":3,"score":0.9803795218467712},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6505013108253479},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.6261902451515198},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.6206575632095337},{"id":"https://openalex.org/C2776441662","wikidata":"https://www.wikidata.org/wiki/Q2823314","display_name":"Tricarboxylic acid","level":4,"score":0.6202260851860046},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.538322925567627},{"id":"https://openalex.org/C66974803","wikidata":"https://www.wikidata.org/wiki/Q10359898","display_name":"Nuclear magnetic resonance spectroscopy","level":2,"score":0.507225513458252},{"id":"https://openalex.org/C160941953","wikidata":"https://www.wikidata.org/wiki/Q6822342","display_name":"Metabolic flux analysis","level":3,"score":0.44937044382095337},{"id":"https://openalex.org/C135870905","wikidata":"https://www.wikidata.org/wiki/Q903667","display_name":"Metabolome","level":3,"score":0.41120877861976624},{"id":"https://openalex.org/C165135838","wikidata":"https://www.wikidata.org/wiki/Q133895","display_name":"Citric acid cycle","level":3,"score":0.3622332215309143},{"id":"https://openalex.org/C147597530","wikidata":"https://www.wikidata.org/wiki/Q369472","display_name":"Computational chemistry","level":1,"score":0.35266822576522827},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.3520636558532715},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.2536408603191376},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.23903244733810425},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.22378897666931152},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.19013020396232605},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1840413212776184},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1302136480808258},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.10191181302070618}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","doi":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.0.co;2-o","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"doi:10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.3.co;2-f","doi":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.3.co;2-f","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0290(19990220)62:4<375::aid-bit1>3.3.co;2-f","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:10099550","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10099550","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.550.959","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.550.959","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://web.mit.edu/biology/sinskey/www/Klapa99.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W14527211","https://openalex.org/W1490420782","https://openalex.org/W1490487466","https://openalex.org/W1525620688","https://openalex.org/W1538994200","https://openalex.org/W1540937567","https://openalex.org/W1555540695","https://openalex.org/W1557317496","https://openalex.org/W1573202329","https://openalex.org/W1588593248","https://openalex.org/W1595569274","https://openalex.org/W1631826549","https://openalex.org/W1915857563","https://openalex.org/W1920989919","https://openalex.org/W1946299058","https://openalex.org/W1973857698","https://openalex.org/W1979027357","https://openalex.org/W1984362846","https://openalex.org/W2000167689","https://openalex.org/W2000240638","https://openalex.org/W2003925921","https://openalex.org/W2006062850","https://openalex.org/W2006128463","https://openalex.org/W2039721323","https://openalex.org/W2058515064","https://openalex.org/W2075397625","https://openalex.org/W2078370861","https://openalex.org/W2096767559","https://openalex.org/W2136811206","https://openalex.org/W2156319838","https://openalex.org/W2160861953","https://openalex.org/W2166924722","https://openalex.org/W2171554775","https://openalex.org/W2263736453","https://openalex.org/W2287671349","https://openalex.org/W2319739930","https://openalex.org/W2323108252","https://openalex.org/W2414109203"],"related_works":["https://openalex.org/W7099984383","https://openalex.org/W2000240638","https://openalex.org/W2281646205","https://openalex.org/W2096767559","https://openalex.org/W2010135382","https://openalex.org/W2005493903","https://openalex.org/W144886596","https://openalex.org/W1968221462","https://openalex.org/W42981771","https://openalex.org/W2001727623"],"abstract_inverted_index":{"Proper":[0],"analysis":[1,154],"of":[2,14,36,38,53,60,62,78,92,108,117,138,155,158],"label":[3,82],"distribution":[4,83],"in":[5],"metabolic":[6,44,63,93],"pathway":[7],"intermediates":[8,64,113],"is":[9,30,84,150],"critical":[10],"for":[11,22,75],"correct":[12],"interpretation":[13],"experimental":[15,19],"data":[16],"and":[17,134],"strategic":[18],"design.":[20],"While,":[21],"example,":[23],"13C":[24,39,118,131],"nuclear":[25],"magnetic":[26],"resonance":[27],"(NMR)":[28],"spectroscopy":[29],"usually":[31],"limited":[32],"to":[33,81,104,129,152],"the":[34,50,76,115,127,148,153],"measurement":[35],"degrees":[37],"enrichment,":[40],"more":[41],"information":[42],"about":[43],"fluxes":[45],"can":[46],"be":[47],"extracted":[48],"from":[49,89],"fine":[51],"structure":[52],"NMR":[54,132],"spectra,":[55],"or":[56],"molecular":[57,135],"weight":[58,136],"distributions":[59,107,137],"isotopomers":[61],"(measured":[65],"by":[66],"gas":[67],"chromatography-mass":[68],"spectrometry).":[69],"For":[70],"this":[71,96],"purpose,":[72],"rigorous":[73],"accounting":[74],"contribution":[77],"all":[79],"pathways":[80],"required,":[85],"especially":[86],"contributions":[87],"resulting":[88],"multiple":[90],"turns":[91],"cycles.":[94],"In":[95,140],"paper":[97,143],"we":[98],"present":[99],"a":[100,141],"mathematical":[101],"model":[102],"developed":[103],"analyze":[105,130],"isotopomer":[106],"tricarboxylic":[109],"acid":[110],"cycle":[111],"(TCA)":[112],"following":[114],"administration":[116],"(or":[119],"14C)":[120],"labeled":[121],"substrates.":[122],"The":[123],"theory":[124,149],"presented":[125],"provides":[126],"basis":[128],"spectra":[133],"metabolites.":[139],"companion":[142],"(Park":[144],"et":[145],"al.,":[146],"1999),":[147],"applied":[151],"several":[156],"cases":[157],"biological":[159],"significance.":[160],"Copyright":[161],"1999":[162],"John":[163],"Wiley":[164],"&":[165],"Sons,":[166],"Inc.":[167]},"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}