{"id":"https://openalex.org/W2017684157","doi":"https://doi.org/10.1373/clinchem.2003.020750","title":"Use of Magnetic Beads for Plasma Cell-free DNA Extraction: Toward Automation of Plasma DNA Analysis for Molecular Diagnostics","display_name":"Use of Magnetic Beads for Plasma Cell-free DNA Extraction: Toward Automation of Plasma DNA Analysis for Molecular Diagnostics","publication_year":2003,"publication_date":"2003-11-01","ids":{"openalex":"https://openalex.org/W2017684157","doi":"https://doi.org/10.1373/clinchem.2003.020750","mag":"2017684157","pmid":"https://pubmed.ncbi.nlm.nih.gov/14578335"},"language":"en","primary_location":{"id":"doi:10.1373/clinchem.2003.020750","doi":"https://doi.org/10.1373/clinchem.2003.020750","is_oa":false,"landing_page_url":"https://doi.org/10.1373/clinchem.2003.020750","pdf_url":null,"source":{"id":"https://openalex.org/S139212996","display_name":"Clinical Chemistry","issn_l":"0009-9147","issn":["0009-9147","1530-8561"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320272","host_organization_name":"American Association for Clinical Chemistry","host_organization_lineage":["https://openalex.org/P4310320272"],"host_organization_lineage_names":["American Association for Clinical Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111664937","display_name":"Christine Stemmer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]},{"id":"https://openalex.org/I4210157127","display_name":"H\u00f4pital d'Hautepierre","ror":"https://ror.org/04e1w6923","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Christine Stemmer","raw_affiliation_strings":["Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076414080","display_name":"Mich\u00e8le Beau\u2010Faller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]},{"id":"https://openalex.org/I4210157127","display_name":"H\u00f4pital d'Hautepierre","ror":"https://ror.org/04e1w6923","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Miche\u0300le Beau-Faller","raw_affiliation_strings":["Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072224218","display_name":"Erwan Pencreach","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]},{"id":"https://openalex.org/I4210157127","display_name":"H\u00f4pital d'Hautepierre","ror":"https://ror.org/04e1w6923","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Erwan Pencreac\u2019h","raw_affiliation_strings":["Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008277545","display_name":"\u00c9ric Gu\u00e9rin","orcid":"https://orcid.org/0000-0003-1388-2834","observed_orcids":["https://orcid.org/0000-0003-1388-2834"]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]},{"id":"https://openalex.org/I4210157127","display_name":"H\u00f4pital d'Hautepierre","ror":"https://ror.org/04e1w6923","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Eric Guerin","raw_affiliation_strings":["Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104060096","display_name":"Anne Schneider","orcid":"https://orcid.org/0009-0003-7327-6446","observed_orcids":["https://orcid.org/0009-0003-7327-6446","https://orcid.org/0009-0002-8444-8410"]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]},{"id":"https://openalex.org/I4210157127","display_name":"H\u00f4pital d'Hautepierre","ror":"https://ror.org/04e1w6923","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Anne Schneider","raw_affiliation_strings":["Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063541966","display_name":"Didier Jaqmin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Didier Jaqmin","raw_affiliation_strings":["Service de Chirugie Urologique and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Chirugie Urologique and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013065628","display_name":"Elizabeth Quoix","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Elizabeth Quoix","raw_affiliation_strings":["Service de Pneumologie Lyautey, Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Pneumologie Lyautey, Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024775717","display_name":"Marie\u2010Pierre Gaub","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]},{"id":"https://openalex.org/I4210157127","display_name":"H\u00f4pital d'Hautepierre","ror":"https://ror.org/04e1w6923","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Marie-Pierre Gaub","raw_affiliation_strings":["Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5053152312","display_name":"Pierre Oudet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210145324","display_name":"H\u00f4pitaux Universitaires de Strasbourg","ror":"https://ror.org/04bckew43","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324"]},{"id":"https://openalex.org/I4210157127","display_name":"H\u00f4pital d'Hautepierre","ror":"https://ror.org/04e1w6923","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Pierre Oudet","raw_affiliation_strings":["Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Service de Biochimie et Biologie Mole\u0301culaire, Ho\u0302pital de Hautepierre Ho\u0302pitaux Universitaires de Strasbourg, 67000 Strasbourg, France","institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111664937"],"corresponding_institution_ids":["https://openalex.org/I4210145324","https://openalex.org/I4210157127"],"apc_list":{"value":4636,"currency":"USD","value_usd":4636},"apc_paid":null,"fwci":0.5989,"has_fulltext":false,"cited_by_count":55,"citation_normalized_percentile":{"value":0.62256158,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"49","issue":"11","first_page":"1953","last_page":"1955"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.9634000062942505,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.9634000062942505,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.01600000075995922,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10978","display_name":"Prenatal Screening and Diagnostics","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/2735","display_name":"Pediatrics, Perinatology and Child Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasma-cell-free-dna","display_name":"plasma cell-free DNA","score":0.878000020980835},{"id":"https://openalex.org/keywords/magnetic-bead-dna-extraction","display_name":"magnetic bead DNA extraction","score":0.6930000185966492},{"id":"https://openalex.org/keywords/liquid-biopsy","display_name":"liquid biopsy","score":0.49000000953674316},{"id":"https://openalex.org/keywords/lung-cancer","display_name":"lung cancer","score":0.6269999742507935},{"id":"https://openalex.org/keywords/colon-cancer","display_name":"colon cancer","score":0.9169999957084656},{"id":"https://openalex.org/keywords/circulating-tumor-dna","display_name":"circulating tumor DNA","score":0.7300000190734863},{"id":"https://openalex.org/keywords/leukocyte-contamination","display_name":"leukocyte contamination","score":0.6520000100135803},{"id":"https://openalex.org/keywords/proteinase-k-digestion","display_name":"proteinase K digestion","score":0.6179999709129333},{"id":"https://openalex.org/keywords/plasma-concentration","display_name":"plasma concentration","score":0.3240000009536743},{"id":"https://openalex.org/keywords/pcr-inhibitors","display_name":"PCR inhibitors","score":0.4000000059604645},{"id":"https://openalex.org/keywords/liquid-biopsy-standardization","display_name":"liquid biopsy standardization","score":0.3199999928474426},{"id":"https://openalex.org/keywords/molecular-diagnosis","display_name":"molecular diagnosis","score":0.4490000009536743}],"concepts":[{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.684141218662262},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6165600419044495},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.6130691170692444},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6001533269882202},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5335199236869812},{"id":"https://openalex.org/C152110520","wikidata":"https://www.wikidata.org/wiki/Q14225730","display_name":"Cell-free fetal DNA","level":5,"score":0.48615095019340515},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.46559035778045654},{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.44528526067733765},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.41984888911247253},{"id":"https://openalex.org/C2776575606","wikidata":"https://www.wikidata.org/wiki/Q79749","display_name":"Blood plasma","level":2,"score":0.41007208824157715},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.34188008308410645},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.32346129417419434},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.25164735317230225},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22655126452445984},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22306469082832336},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.17070487141609192},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09096291661262512},{"id":"https://openalex.org/C2778258057","wikidata":"https://www.wikidata.org/wiki/Q1136569","display_name":"Prenatal diagnosis","level":4,"score":0.0},{"id":"https://openalex.org/C2779234561","wikidata":"https://www.wikidata.org/wiki/Q11995","display_name":"Pregnancy","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C172680121","wikidata":"https://www.wikidata.org/wiki/Q26513","display_name":"Fetus","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001322","descriptor_name":"Autoanalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003110","descriptor_name":"Colonic Neoplasms","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D008280","descriptor_name":"Magnetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010949","descriptor_name":"Plasma","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018895","descriptor_name":"Microsatellite Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1373/clinchem.2003.020750","doi":"https://doi.org/10.1373/clinchem.2003.020750","is_oa":false,"landing_page_url":"https://doi.org/10.1373/clinchem.2003.020750","pdf_url":null,"source":{"id":"https://openalex.org/S139212996","display_name":"Clinical Chemistry","issn_l":"0009-9147","issn":["0009-9147","1530-8561"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320272","host_organization_name":"American Association for Clinical Chemistry","host_organization_lineage":["https://openalex.org/P4310320272"],"host_organization_lineage_names":["American Association for Clinical Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Chemistry","raw_type":"journal-article"},{"id":"pmid:14578335","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/14578335","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W1506214019","https://openalex.org/W1645433395","https://openalex.org/W1996593999","https://openalex.org/W2023846436","https://openalex.org/W2029830472","https://openalex.org/W2057200145","https://openalex.org/W2092479048","https://openalex.org/W2096643260","https://openalex.org/W2101037404","https://openalex.org/W2108546855","https://openalex.org/W2108677652","https://openalex.org/W2131641641","https://openalex.org/W2145548425","https://openalex.org/W2168153771","https://openalex.org/W2199277449","https://openalex.org/W2606963233"],"related_works":["https://openalex.org/W2091742509","https://openalex.org/W4306916573","https://openalex.org/W2317656106","https://openalex.org/W4410189909","https://openalex.org/W1542945437","https://openalex.org/W2101037404","https://openalex.org/W4411006668","https://openalex.org/W2093673462","https://openalex.org/W2060633466","https://openalex.org/W1589540250"],"abstract_inverted_index":{"Urine,":[0],"breast":[1],"milk,":[2],"plasma,":[3,261,607],"and":[4,27,49,58,63,129,176,199,227,252,263,324,388,418,456,503,561,584,593,652,656,687,740,745,749,796,821,1023,1050,1069,1089,1148,1168,1178,1310,1355,1414,1432,1464,1479,1525,1540,1549,1679,1692,1711,1740,1748,1763,1772,1774,1784,1838,1848,1855],"serum":[5],"have":[6],"been":[7],"shown":[8,756],"to":[9,91,100,205,222,259,334,367,415,449,493,499,507,526,614,626,732,816,982,1013,1040,1053,1076,1092,1157,1436,1553,1564,1643,1655,1666,1710],"contain":[10],"cell-free":[11,123,1614],"DNA":[12,16,61,74,124,143,150,307,314,338,357,389,401,409,448,452,695,710,828,870,917,925,933,1126,1155,1240,1259,1313,1333,1362,1390,1417,1427,1475,1486,1500,1538,1542,1575,1593,1616,1623,1695,1706,1730,1800],"(1)(2)(3)(4)(5)(6)(7).":[13],"For":[14,355,397,616,949,1421],"plasma":[15,41,98,122,139,149,202,272,356,372,400,440,479,521,576,622,636,714,718,764,916,924,1012,1182,1389,1474,1499,1523,1537,1592,1688,1799],"detection,":[17],"several":[18,946],"recent":[19],"studies":[20,947],"addressed":[21],"the":[22,46,64,95,200,260,335,353,364,407,416,429,468,472,478,484,494,500,504,508,517,520,543,547,552,565,603,608,642,673,691,699,702,767,774,790,797,805,810,818,840,882,912,950,983,988,1019,1028,1055,1093,1101,1130,1136,1142,1145,1164,1196,1207,1222,1232,1244,1266,1277,1449,1454,1470,1481,1507,1516,1588,1628,1639,1649,1670,1674,1680,1742,1757,1786],"need":[23],"for":[24,80,119,132,165,189,196,212,287,394,454,467,649,659,793,837,879,1002,1011,1176,1307,1314,1322,1325,1349,1359,1434,1522,1569,1587,1606,1728,1851,1858],"careful":[25],"evaluation":[26],"standardization":[28],"of":[29,40,52,66,73,82,94,97,121,148,160,217,232,319,370,378,385,406,435,439,446,458,471,477,496,519,555,606,621,635,685,690,762,823,827,869,915,932,938,987,1016,1043,1058,1073,1079,1104,1116,1125,1144,1151,1154,1180,1191,1198,1209,1257,1335,1341,1352,1371,1388,1400,1423,1440,1498,1515,1577,1585,1600,1613,1687,1694,1699,1704,1754,1759,1766,1798],"preanalytical":[30],"processes":[31],"(8)(9)(10)(11)(12).":[32],"Key":[33],"problems":[34],"appear,":[35],"such":[36,1298],"as":[37,350,862,866,904,908,1299,1363,1374,1501],"possible":[38],"contamination":[39,1192,1210],"by":[42,152,276,341,352,428,474,483,766,845,854,887,896,1006,1046,1114,1195,1365,1376,1398,1551,1811],"white":[43],"blood":[44,156],"cells;":[45],"generally":[47],"low":[48],"variable":[50],"amount":[51,931],"circulating":[53,1615],"DNA,":[54,522,1590],"making":[55],"extraction/quantification":[56],"difficult":[57,90],"time-consuming;":[59],"poor":[60],"quality;":[62],"presence":[65],"PCR":[67,133,563,1220,1262,1291,1412,1424,1435,1444,1637,1782],"inhibitors.":[68],"In":[69,104,1118],"any":[70,224],"case,":[71],"automation":[72,1693],"extraction,":[75,358],"which":[76],"is":[77,89,130,942,1609,1745],"a":[78,114,206,325,995,1080,1097,1212,1219,1225,1286,1311,1368,1484,1574,1582,1760,1793,1803],"prerequisite":[79],"introduction":[81],"these":[83,801,967,1074,1271,1323,1570,1607,1658],"diagnostic":[84],"approaches":[85],"in":[86,108,182,292,363,392,460,546,596,698,757,943,1107,1129,1231,1247,1270,1276,1285,1409,1448,1473,1503,1506,1526,1636,1669,1802],"clinical":[87,1805],"laboratories,":[88],"achieve":[92],"because":[93,966],"volumes":[96,1072,1103],"necessary":[99],"get":[101],"sufficient":[102],"DNA.":[103],"this":[105,556,940],"study":[106],"(summarized":[107],"Fig.":[109,568,1446,1504],"1":[110,265,310,511,569,657,759,1077,1496],"),":[111],"we":[112,523,716,922,956,1035,1061,1099,1121,1216,1329,1518,1534,1788],"propose":[113],"new":[115],"semiautomated,":[116],"time-saving":[117],"process":[118,1138,1175,1246,1723,1787],"extraction":[120,308,711,734,761,1056,1167,1177,1308,1340,1731],"that":[125,550,672,804,1034,1135,1619,1685],"provides":[126],"high":[127,674,806,1152],"yields":[128,712,772,807,829,871,1111,1708],"suitable":[131],"amplification.":[134,1204],"Experimental":[135],"design.":[136],"Step":[137],"1,":[138],"treatment;":[140],"step":[141,145,216,312,513,571,820,1795],"2,":[142],"extraction;":[144],"3,":[146],"analysis":[147],"quality":[151,518,1296,1387],"PCR.":[153,1557,1647],"Two":[154],"5-mL":[155],"samples":[157,273,441,473,525,577,719,765,795,1183,1241,1273,1524,1539,1571,1689],"from":[158,578,612,701,720,926,953,1064,1367,1419,1476,1488,1594,1653],"each":[159],"23":[161],"patients":[162,580,723,968,1478,1596],"being":[163],"monitored":[164],"lung":[166],"(n":[167,173,581,587,629,639],"=":[168,174,582,588,630,640,779,787],"19)":[169],"or":[170,1302,1645],"colon":[171],"cancer":[172,579,722,954],"4)":[175],"20":[177,238,288,395,927,1238,1342,1350,1586],"healthy":[178,585,928],"controls":[179,586,1297,1379,1529,1767],"were":[180,274,301,361,426,491,963,969,1085,1112,1171,1317,1380,1776],"collected":[181,201,952],"EDTA-containing":[183],"tubes.":[184],"Informed":[185],"consent":[186],"was":[187,192,203,257,315,339,373,390,402,465,637,646,696,730,738,747,843,852,885,894,935,980,1184,1193,1395,1428,1520,1562,1809],"obtained":[188,808,830,872,918,957,1036,1330,1391,1459,1492,1519,1581,1716],"each.":[190],"Blood":[191],"centrifuged":[193],"at":[194,220,267,278,290,303,559,971,1018,1027,1048,1260,1638],"800g":[195,1049],"10":[197,218,247],"min,":[198],"transferred":[204],"15-mL":[207],"BD":[208],"FalconTM":[209],"polypropylene":[210],"tube":[211],"an":[213,536,1065,1320],"additional":[214,1199,1736],"centrifugation":[215,277,989,997,1000,1009,1047],"min":[219,289],"1500g":[221],"remove":[223],"remaining":[225],"leukocytes":[226,1017,1045,1063,1105,1304],"platelets.":[228],"An":[229],"equal":[230],"volume":[231,605,645],"2\u00d7":[233],"concentrated":[234,275,299,371,594,644,739,748,763,794],"proteolytic":[235],"buffer":[236,380,482],"(2\u00d7:":[237],"mmol/L":[239,242,245],"Tris-HCl,":[240],"50":[241],"EDTA,":[243],"200":[244,680],"NaCl,":[246],"g/L":[248],"sodium":[249,283],"dodecyl":[250,284],"sulfate,":[251],"400":[253],"mg/L":[254],"proteinase":[255,591,668,675],"K)":[256],"added":[258,1070,1146,1381],"mixed,":[262],"incubated":[264],"h":[266],"37":[268],"\u00b0C.":[269],"After":[270],"digestion,":[271],"room":[279],"temperature":[280],"(to":[281],"avoid":[282],"sulfate":[285],"precipitation)":[286],"2600g":[291],"Amicon":[293,597,692,703],"Ultra-15":[294],"filtration":[295,599],"devices":[296,704],"(Millipore).":[297],"The":[298,422,488,930,1031,1173,1189,1378,1386,1511,1602,1677,1697,1721],"plasmas":[300,951],"stored":[302],"\u221220":[304],"\u00b0C":[305],"until":[306],"(Fig.":[309,510,1229,1274],",":[311,512,570,760],"1).":[313],"extracted":[316,403,447,741,750,923,934,1090,1100,1242,1418,1487,1545],"with":[317,345,375,382,404,480,590,751,809,831,873,919,945,1211,1243,1392,1416,1430,1460,1483,1495,1546,1627,1707,1717,1780],"use":[318,405,822,1115,1698,1758,1765],"KingFisher":[320,326,436,752,768,798,811,1094],"silicate":[321],"magnetic":[322,329,386,753,824,920,1059,1165,1547,1700],"beads":[323,1548,1701],"ML":[327],"robotic":[328],"particle":[330,501],"processor":[331,502],"(ThermoLifeSciences)":[332],"according":[333,414,1091],"manufacturer\u2019s":[336],"protocol.":[337,421,1095],"quantified":[340,1364,1429,1550],"fluorometry":[342],"(Fluoroskan;":[343],"ThermoLifeSciences)":[344],"Picogreen":[346,459,505,1315,1366,1431],"reagent":[347,411],"(Molecular":[348],"Probes)":[349],"recommended":[351],"manufacturer.":[354],"slight":[359],"modifications":[360],"introduced":[362],"protocol:":[365,998],"up":[366,1218],"300":[368],"\u03bcL":[369,377,384,628,648,655,1351],"mixed":[374],"950":[376],"lysis":[379,443,481,1015],"together":[381],"80":[383],"beads,":[387,921,1060],"eluted":[391],"Tris-EDTA":[393],"min.":[396],"comparison":[398,838,880,1415,1422],"purposes,":[399],"QIAamp":[408],"Midi":[410],"set":[412,1217],"(Qiagen),":[413],"\u201cblood":[417],"body":[419],"fluid\u201d":[420],"following":[423],"pipetting":[424,434,476,1761],"steps":[425,490,990,1170,1737],"performed":[427,1185],"Multiprobe":[430,485],"II":[431,486],"workstation":[432,1762],"(Perkin-Elmer):":[433],"reagents,":[437],"transfer":[438,445,495],"into":[442,724],"buffer,":[444],"storage":[450],"tubes,":[451],"dilutions":[453],"quantification,":[455],"distribution":[457],"96-well":[461],"plates.":[462],"Special":[463,978],"care":[464],"taken":[466],"complete":[469,1174],"homogenization":[470],"up-and-down":[475],"robot.":[487],"manual":[489,1752],"reduced":[492,625],"tube-strip":[497],"trays":[498],"plate":[506],"fluorometer":[509],"2).":[514],"To":[515,708,910,1160,1205],"evaluate":[516,911],"subjected":[524,731,1535],"microsatellite":[527,1203,1402,1646],"amplification":[528,1256,1399],"using":[529,1224],"fluorescent":[530],"primers,":[531],"sequencing":[532,1644],"[both":[533],"analyzed":[534],"on":[535,564,602,742,1221,1265,1573],"ABI":[537],"Prism":[538],"3100":[539],"(Applied":[540],"Biosystems);":[541],"see":[542],"detailed":[544],"method":[545,769,792,812],"Data":[548,1132,1234,1279,1451,1509],"Supplement":[549,1452],"accompanies":[551],"online":[553,1131,1233,1278,1450,1508],"version":[554],"Technical":[557],"Brief":[558],"http://www.clinchem.org/content/vol49/issue11/],":[560],"real-time":[562],"LightCycler":[566,1223],"(Roche;":[567],"3).":[572],"We":[573,781,993,1236,1253,1467,1491,1580,1844],"digested":[574],"43":[575],"23)":[583],"20)":[589],"K":[592,669,676],"them":[595],"Ultra":[598],"devices.":[600],"Depending":[601],"initial":[604,972],"concentration":[609,663,677,860,902],"factor":[610],"varied":[611],"4":[613,633],"10.":[615],"all":[617,1283,1426,1544,1621],"cases,":[618],"2":[619,1128,1158],"mL":[620,634,658,1078],"could":[623,664,1662,1724],"be":[624,665,814,1038,1625,1634,1664,1726,1792],"150\u2013250":[627],"15).":[631],"When":[632],"used":[638,1563,1727],"27),":[641],"final":[643],"\u2264500":[647],"15":[650],"cases":[651],"between":[653,789],"600":[654],"12":[660,1326],"samples.":[661,1252],"No":[662,694,960],"achieved":[666],"without":[667,735],"incubation,":[670],"suggesting":[671],"(final":[678],"concentration,":[679,715,736],"mg/L)":[681],"allows":[682,1149,1702],"efficient":[683,1041],"digestion":[684,1739],"proteins":[686],"avoids":[688],"clogging":[689],"filter.":[693],"detected":[697,1254],"filtrates":[700],"(data":[705,1292,1530,1732],"not":[706,970,1140,1293,1531,1620,1733],"shown).":[707,1294,1532,1734],"analyze":[709],"after":[713,1202,1339],"divided":[717],"16":[721],"three":[725,832,841,874,883],"1-mL":[726],"aliquots:":[727],"one":[728,737,746,1541],"aliquot":[729],"Qiagen":[733,743,776,791],"columns,":[744],"beads.":[754,825],"As":[755,1096,1319],"Table":[758],"gave":[770,1597],"higher":[771,961,1007,1020,1659],"than":[773,1714,1751],"classic":[775],"protocol":[777,1394,1528,1678,1744],"(P":[778,786,848,890],"0.009).":[780],"observed":[782],"no":[783,1255],"significant":[784],"difference":[785],"0.421)":[788],"method.":[799],"Overall":[800],"results":[802,1681],"suggest":[803],"may":[813,1037,1651,1791],"attributable":[815,1039,1665],"both":[817,1119],"digestion/concentration":[819],"Comparison":[826,868],"pretreatment/extraction":[833,875],"methods.1":[834,876],"Statistical":[835,877],"significance":[836,851,878,893],"among":[839,881],"groups":[842,884,1120],"calculated":[844,853,886,895],"Friedman":[846,888],"ANOVA":[847,857,889,899],"<0.001).":[849,891],"b,cStatistical":[850,892],"bilateral":[855,897],"Dunett":[856,898],"with:":[858,900],"bQiagen/no":[859,901],"group":[861,865,903,907],"reference;":[863,905],"cKingFisher":[864,906],"reference.":[867,909],"background":[913],"concentrations":[914,1334,1686],"controls.":[929],"3\u201322":[936],"ng/mL":[937],"plasma;":[939],"range":[941],"agreement":[944],"(13)(14)(15).":[948],"patients,":[955],"13\u2013127":[958],"ng/mL.":[959],"values":[962,1033,1599,1661],"observed,":[964],"probably":[965],"diagnosis":[973],"but":[974],"under":[975],"treatment":[976],"(15).":[977],"attention":[979],"paid":[981],"already":[984],"noticed":[985],"importance":[986],"before":[991],"extraction.":[992,1696],"designed":[994],"two-step":[996],"low-speed":[999],"(800g)":[1001],"whole":[1003,1137,1743],"blood,":[1004],"followed":[1005],"speed":[1008,1021],"(1500g)":[1010],"avoided":[1014],"(11)":[1022],"insufficient":[1024],"cell":[1025],"clearance":[1026],"lower":[1029,1032],"speed.":[1030],"elimination":[1042],"contaminating":[1044],"1500g.":[1051],"Finally,":[1052,1533,1657],"assess":[1054],"capacity":[1057],"prepared":[1062],"unique":[1066],"buffy":[1067,1353],"coat":[1068],"different":[1071,1411,1595],"cells":[1075,1301],"control":[1081,1287,1303,1309,1485,1589],"plasma.":[1082],"These":[1083],"mixtures":[1084],"then":[1086],"digested,":[1087],"concentrated,":[1088],"control,":[1098,1543],"same":[1102,1629],"kept":[1106],"phosphate-buffered":[1108],"saline.":[1109],"Extraction":[1110],"estimated":[1113],"Picogreen.":[1117],"found":[1122],"similar":[1123,1461,1642],"quantities":[1124],"(Table":[1127],"Supplement),":[1133,1280],"showing":[1134],"does":[1139],"affect":[1141],"recovery":[1143],"material":[1147],"purification":[1150],"amounts":[1153],"(up":[1156],"\u03bcg).":[1159],"limit":[1161],"human":[1162],"intervention,":[1163],"bead":[1166],"quantification":[1169,1179,1316],"automated.":[1172],"30":[1181],"within":[1186],"2.5":[1187],"h.":[1188],"absence":[1190,1197,1208],"checked":[1194],"detectable":[1200],"peaks":[1201],"confirm":[1206],"more":[1213,1749],"sensitive":[1214],"approach,":[1215],"chromosome":[1226,1267],"Y":[1227,1268],"marker":[1228,1269],"2A":[1230],"Supplement).":[1235],"amplified":[1237,1284,1626],"female":[1239,1272],"automated":[1245,1327,1384,1781],"experiments":[1248],"also":[1249,1663,1725],"including":[1250],"male":[1251,1258],"45":[1261],"cycles":[1263,1567],"based":[1264,1572],"2B":[1275],"whereas":[1281,1591],"they":[1282],"glyceraldehyde-3-phosphate":[1288],"dehydrogenase":[1289],"(GAPDH)":[1290],"Systematic":[1295],"cultured":[1300,1344],"(buffy":[1305],"coat)":[1306],"known":[1312],"used.":[1318],"example,":[1321],"controls,":[1324],"extractions":[1328],"mean":[1331,1512],"(SD)":[1332],"1.4":[1336],"(0.29)":[1337],"ng/\u03bcL":[1338,1348,1358,1438],"000":[1343],"cells,":[1345],"14":[1346],"(3.8)":[1347],"coat,":[1354],"96":[1356],"(16)":[1357],"normal":[1360,1489],"leukocyte":[1361],"stock":[1369],"solution":[1370],"100":[1372],"ng/\u03bcL,":[1373],"determined":[1375],"spectrophotometry.":[1377],"once":[1382],"per":[1383,1443],"process.":[1385],"our":[1393,1527],"first":[1396,1794],"assessed":[1397],"eight":[1401,1410,1477],"markers":[1403],"(PCR":[1404],"products":[1405],"length,":[1406],"100\u2013250":[1407],"bp":[1408],"reactions)":[1413],"leukocytes.":[1420,1490],"profiles,":[1425],"normalized":[1433],"0.125":[1437],"(input":[1439],"0.5":[1441],"ng":[1442,1497],"reaction).":[1445],"3A":[1447],"summarizes":[1453],"very":[1455],"close":[1456],"profiles":[1457],"routinely":[1458],"peak":[1462,1465],"heights":[1463],"shapes.":[1466],"next":[1468],"sequenced":[1469],"K-ras":[1471],"gene":[1472],"compared":[1480],"sequences":[1482,1494,1517],"high-quality":[1493,1705],"exemplified":[1502],"3B":[1505],"Supplement.":[1510],"raw":[1513],"signal":[1514],"250":[1521],"six":[1536],"Picogreen,":[1552],"SYBR":[1554],"Green":[1555],"I":[1556],"A":[1558],"110-bp":[1559],"GAPDH":[1560,1675],"product":[1561],"compare":[1565],"threshold":[1566],"(Ct)":[1568],"input":[1576],"3":[1578],"ng/PCR.":[1579],"Ct":[1583,1598,1660],"value":[1584],"22\u201325.":[1601],"most":[1603],"likely":[1604],"hypothesis":[1605],"differences":[1608],"partial":[1610],"physiologic":[1611],"degradation":[1612,1650],"(16),":[1617],"meaning":[1618],"extracted/quantified":[1622],"can":[1624],"efficiency.":[1630],"This":[1631,1807],"phenomenon":[1632],"cannot":[1633],"visualized":[1635],"plateau":[1640],"phase,":[1641],"Moreover,":[1648,1756],"vary":[1652],"patient":[1654],"patient.":[1656],"chromosomal":[1667],"deletions":[1668],"genomic":[1671],"region":[1672],"bearing":[1673],"gene.":[1676],"described":[1682,1719,1722],"above":[1683],"show":[1684],"allow":[1690],"miniaturization":[1691],"\u201cfishing-out\u201d":[1703],"comparable":[1709],"even":[1712],"better":[1713],"those":[1715],"commonly":[1718],"techniques.":[1720],"urinary":[1729],"Despite":[1735],"(proteolytic":[1738],"concentration),":[1741],"easily":[1746],"scalable":[1747],"reliable":[1750],"handling":[1753],"columns.":[1755],"systematic":[1764],"considerably":[1768],"decreased":[1769],"error":[1770],"rates,":[1771],"precision":[1773],"reproducibility":[1775],"much":[1777],"higher.":[1778],"Combined":[1779],"preparation":[1783],"analysis,":[1785],"describe":[1789],"here":[1790],"toward":[1796],"rationalization":[1797],"detection":[1801],"routine":[1804],"setting.":[1806],"work":[1808],"supported":[1810],"La":[1812],"Ligue":[1813],"contre":[1814],"le":[1815],"Cancer":[1816],"Comite\u0301":[1817],"du":[1818],"Haut-Rhin,":[1819],"la":[1820,1823],"Fondation":[1821],"pour":[1822],"Recherche":[1824],"Me\u0301dicale,":[1825],"Conseil":[1826,1829],"Re\u0301gional":[1827],"d\u2019Alsace,":[1828],"Ge\u0301ne\u0301ral,":[1830],"Communaute\u0301":[1831],"Urbaine":[1832],"de":[1833,1842],"Strasbourg,":[1834],"Assureurs":[1835],"Pre\u0301vention":[1836],"Sante\u0301,":[1837],"Les":[1839],"Ho\u0302pitaux":[1840],"Universitaires":[1841],"Strasbourg.":[1843],"thank":[1845],"V.":[1846],"Kussaibi":[1847],"O.":[1849],"Nullans":[1850],"excellent":[1852],"technical":[1853],"assistance":[1854],"T.":[1856],"Lavaux":[1857],"helpful":[1859],"discussions.":[1860]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}