{"id":"https://openalex.org/W2017675208","doi":"https://doi.org/10.1111/j.1365-3083.1991.tb01582.x","title":"Formation of Covalent C3b\u2010Tetanus Toxin Complexes: a Tool for the In Vitro Study of Antigen Presentation","display_name":"Formation of Covalent C3b\u2010Tetanus Toxin Complexes: a Tool for the In Vitro Study of Antigen Presentation","publication_year":1991,"publication_date":"1991-11-01","ids":{"openalex":"https://openalex.org/W2017675208","doi":"https://doi.org/10.1111/j.1365-3083.1991.tb01582.x","mag":"2017675208","pmid":"https://pubmed.ncbi.nlm.nih.gov/1947794"},"language":"en","primary_location":{"id":"doi:10.1111/j.1365-3083.1991.tb01582.x","doi":"https://doi.org/10.1111/j.1365-3083.1991.tb01582.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-3083.1991.tb01582.x","pdf_url":null,"source":{"id":"https://openalex.org/S79173686","display_name":"Scandinavian Journal of Immunology","issn_l":"0300-9475","issn":["0300-9475","1365-3083"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scandinavian Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111767898","display_name":"Marie\u2010Bernadette Villiers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"M.B. VILLIERS*","raw_affiliation_strings":["DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]},{"raw_affiliation_string":"DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018858147","display_name":"Christian L. VILLIERS","orcid":"https://orcid.org/0000-0002-6548-278X","observed_orcids":["https://orcid.org/0000-0002-6548-278X"]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"C. L. VILLIERS","raw_affiliation_strings":["DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]},{"raw_affiliation_string":"DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075244777","display_name":"John Fraser Wright","orcid":"https://orcid.org/0000-0001-9635-1750","observed_orcids":["https://orcid.org/0000-0001-9635-1750"]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"J. F. WRIGHT","raw_affiliation_strings":["DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]},{"raw_affiliation_string":"DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016789115","display_name":"Christ\u00e8le Maison","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"C. M. MAISON","raw_affiliation_strings":["DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]},{"raw_affiliation_string":"DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039426717","display_name":"Maurice G. Colomb","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"M. G. COLOMB","raw_affiliation_strings":["DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DBMS/ICH. Unite\u0301 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]},{"raw_affiliation_string":"DBMS/ICH. Unit\u00e9 INSERM 238. Grenoble, France","institution_ids":["https://openalex.org/I154526488"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5018858147"],"corresponding_institution_ids":["https://openalex.org/I154526488"],"apc_list":{"value":4480,"currency":"USD","value_usd":4480},"apc_paid":null,"fwci":0.9599,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.73046099,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"34","issue":"5","first_page":"585","last_page":"595"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/c3b","display_name":"C3b","score":0.781000018119812},{"id":"https://openalex.org/keywords/tetanus-toxin","display_name":"tetanus toxin","score":0.9980000257492065},{"id":"https://openalex.org/keywords/complement-system","display_name":"complement system","score":0.42100000381469727},{"id":"https://openalex.org/keywords/antigen-presentation","display_name":"antigen presentation","score":0.9279999732971191},{"id":"https://openalex.org/keywords/hydroxylamine","display_name":"hydroxylamine","score":0.824999988079071},{"id":"https://openalex.org/keywords/mercuric-chloride","display_name":"mercuric chloride","score":0.8130000233650208},{"id":"https://openalex.org/keywords/sulfhydryl-reagents","display_name":"sulfhydryl reagents","score":0.6769999861717224},{"id":"https://openalex.org/keywords/antigen-presenting-cells","display_name":"antigen-presenting cells","score":0.8299999833106995}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7555526494979858},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.6797575354576111},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.6566203832626343},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.5222290754318237},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4981520175933838},{"id":"https://openalex.org/C2777367657","wikidata":"https://www.wikidata.org/wiki/Q184651","display_name":"Toxin","level":2,"score":0.45826536417007446},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.444631427526474},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41435837745666504},{"id":"https://openalex.org/C102931765","wikidata":"https://www.wikidata.org/wiki/Q189673","display_name":"Dissociation (chemistry)","level":2,"score":0.4124220013618469},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.41212019324302673},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.16640812158584595},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.14568820595741272},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12785762548446655},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.09502044320106506},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000938","descriptor_name":"Antigen-Presenting Cells","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D000941","descriptor_name":"Antigens","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D003179","descriptor_name":"Complement C3b","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008627","descriptor_name":"Mercuric Chloride","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013744","descriptor_name":"Tetanus Toxin","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1365-3083.1991.tb01582.x","doi":"https://doi.org/10.1111/j.1365-3083.1991.tb01582.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-3083.1991.tb01582.x","pdf_url":null,"source":{"id":"https://openalex.org/S79173686","display_name":"Scandinavian Journal of Immunology","issn_l":"0300-9475","issn":["0300-9475","1365-3083"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scandinavian Journal of Immunology","raw_type":"journal-article"},{"id":"pmid:1947794","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1947794","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scandinavian journal of immunology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4000000059604645,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320893","display_name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","ror":"https://ror.org/02vjkv261"},{"id":"https://openalex.org/F4320334904","display_name":"National Institute for Materials Science","ror":"https://ror.org/026v1ze26"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W55978131","https://openalex.org/W90889544","https://openalex.org/W93590824","https://openalex.org/W112786708","https://openalex.org/W183326179","https://openalex.org/W205159189","https://openalex.org/W1240506907","https://openalex.org/W1253835855","https://openalex.org/W1519274415","https://openalex.org/W1526898493","https://openalex.org/W1527643370","https://openalex.org/W1559636432","https://openalex.org/W1563523590","https://openalex.org/W1565096783","https://openalex.org/W1569807769","https://openalex.org/W1569852506","https://openalex.org/W1600693909","https://openalex.org/W1625660792","https://openalex.org/W1841868345","https://openalex.org/W1870005350","https://openalex.org/W1903537363","https://openalex.org/W1916896845","https://openalex.org/W1980814164","https://openalex.org/W1984417936","https://openalex.org/W1985143918","https://openalex.org/W2004510339","https://openalex.org/W2021807514","https://openalex.org/W2041671003","https://openalex.org/W2043403577","https://openalex.org/W2044531415","https://openalex.org/W2047841570","https://openalex.org/W2061721681","https://openalex.org/W2066195227","https://openalex.org/W2072507785","https://openalex.org/W2072591694","https://openalex.org/W2078247215","https://openalex.org/W2080580789","https://openalex.org/W2100837269","https://openalex.org/W2101367047","https://openalex.org/W2111817213","https://openalex.org/W2119586900","https://openalex.org/W2120762448","https://openalex.org/W2170810910","https://openalex.org/W2238363303","https://openalex.org/W2463439049","https://openalex.org/W2764690639","https://openalex.org/W4239639553","https://openalex.org/W6608344399"],"related_works":["https://openalex.org/W1980644661","https://openalex.org/W2062793155","https://openalex.org/W632005921","https://openalex.org/W254393295","https://openalex.org/W2045493401","https://openalex.org/W2073080466","https://openalex.org/W1800361738","https://openalex.org/W368046238","https://openalex.org/W7098738853","https://openalex.org/W2067964440"],"abstract_inverted_index":{"A":[0],"novel":[1],"method":[2],"is":[3,31],"described":[4],"for":[5],"the":[6,14,47,64,96,103,112,129,142,151,154,174],"formation":[7,65,115],"and":[8,18,44,71,76,101,118,182],"purification":[9],"of":[10,38,42,49,66,74,99,108,116,128,145,153,157,163,176],"covalent":[11,67,81],"complexes":[12,83,167],"between":[13,95],"complement":[15],"component":[16],"C3b":[17,30,68,75,100,117,159,178],"an":[19],"antigen":[20,180],"(tetanus":[21],"toxin,":[22],"TT),":[23],"using":[24,87],"purified":[25,86],"proteins":[26],"in":[27,33,46,113],"fluid":[28],"phase.":[29],"generated":[32],"situ":[34,114],"by":[35,122,135,185],"tryptic":[36],"cleavage":[37],"C3":[39,43],"after":[40],"co-precipitation":[41],"TT":[45,77],"presence":[48],"polyethylene":[50],"glycol.":[51],"Various":[52],"parameters":[53],"were":[54,78,85],"analysed":[55],"to":[56,172],"optimize":[57],"complex":[58,130],"formation;":[59],"under":[60],"conditions":[61],"which":[62,84,140],"minimized":[63],"multimers,":[69],"30%":[70],"8%":[72],"respectively":[73],"incorporated":[79],"into":[80],"one-to-one":[82],"gel":[88],"filtration":[89],"chromatography.":[90],"The":[91],"linkage":[92],"was":[93,119],"localized":[94],"alpha'":[97],"chain":[98,107],"either":[102],"H":[104],"or":[105],"L":[106],"TT;":[109],"it":[110],"required":[111],"partially":[120],"destroyed":[121],"1":[123],"M":[124],"hydroxylamine.":[125],"Spontaneous":[126],"dissociation":[127],"could":[131],"be":[132],"partly":[133],"avoided":[134],"HgCl2,":[136],"a":[137,169],"thiol":[138],"reagent":[139],"inhibits":[141],"esterase-like":[143],"activity":[144],"bound":[146],"C3b.":[147],"These":[148],"findings":[149],"suggest":[150],"involvement":[152],"reactive":[155],"carbonyl":[156],"nascent":[158],"with":[160],"hydroxyl":[161],"groups":[162],"TT.":[164],"Such":[165],"C3b-TT":[166],"provide":[168],"defined":[170],"tool":[171],"analyse":[173],"influence":[175],"antigen-bound":[177],"on":[179],"addressing":[181],"intracellular":[183],"processing":[184],"antigen-presenting":[186],"cells.":[187]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}