{"id":"https://openalex.org/W2017648100","doi":"https://doi.org/10.1021/bi00159a008","title":"A .beta.-turn is present in the 392-411 segment of the human fibrinogen .gamma.-chain. Effects of structural changes in this segment on affinity to antibody 4A5","display_name":"A .beta.-turn is present in the 392-411 segment of the human fibrinogen .gamma.-chain. Effects of structural changes in this segment on affinity to antibody 4A5","publication_year":1992,"publication_date":"1992-11-01","ids":{"openalex":"https://openalex.org/W2017648100","doi":"https://doi.org/10.1021/bi00159a008","mag":"2017648100","pmid":"https://pubmed.ncbi.nlm.nih.gov/1420184"},"language":"en","primary_location":{"id":"doi:10.1021/bi00159a008","doi":"https://doi.org/10.1021/bi00159a008","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00159a008","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045600512","display_name":"Michael Blumenstein","orcid":"https://orcid.org/0000-0002-9908-3744","observed_orcids":["https://orcid.org/0000-0002-9908-3744"]},"institutions":[{"id":"https://openalex.org/I39694355","display_name":"Hunter College","ror":"https://ror.org/00g2xk477","country_code":"US","type":"education","lineage":["https://openalex.org/I39694355"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael Blumenstein","raw_affiliation_strings":["Department of Chemistry, Hunter College, New York, New York 10021"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Hunter College, New York, New York 10021","institution_ids":["https://openalex.org/I39694355"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004551189","display_name":"Gary R. Matsueda","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gary R. Matsueda","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110147376","display_name":"Sheila Timmons","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sheila Timmons","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5087565676","display_name":"Jacek Hawiger","orcid":"https://orcid.org/0000-0003-2721-6859","observed_orcids":["https://orcid.org/0000-0003-2721-6859"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jacek Hawiger","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":5.1456,"has_fulltext":false,"cited_by_count":40,"citation_normalized_percentile":{"value":0.95468782,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"31","issue":"44","first_page":"10692","last_page":"10698"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11721","display_name":"Blood properties and coagulation","score":0.6693999767303467,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11721","display_name":"Blood properties and coagulation","score":0.6693999767303467,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.21449999511241913,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.08990000188350677,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fibrinogen-gamma-chain","display_name":"fibrinogen gamma chain","score":0.9229999780654907},{"id":"https://openalex.org/keywords/beta-turn","display_name":"beta-turn","score":0.753000020980835},{"id":"https://openalex.org/keywords/peptide-conformation","display_name":"peptide conformation","score":0.6380000114440918},{"id":"https://openalex.org/keywords/antiplatelet-drugs","display_name":"antiplatelet agents","score":0.878000020980835},{"id":"https://openalex.org/keywords/ph-dependent-conformation","display_name":"pH-dependent conformation","score":0.5839999914169312},{"id":"https://openalex.org/keywords/steric-effects","display_name":"steric effects","score":0.7279999852180481}],"concepts":[{"id":"https://openalex.org/C2776174256","wikidata":"https://www.wikidata.org/wiki/Q830842","display_name":"BETA (programming language)","level":2,"score":0.5717532634735107},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5584445595741272},{"id":"https://openalex.org/C2779036427","wikidata":"https://www.wikidata.org/wiki/Q381899","display_name":"Fibrinogen","level":2,"score":0.49578580260276794},{"id":"https://openalex.org/C199185054","wikidata":"https://www.wikidata.org/wiki/Q552299","display_name":"Chain (unit)","level":2,"score":0.48790737986564636},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4874856472015381},{"id":"https://openalex.org/C85641259","wikidata":"https://www.wikidata.org/wiki/Q290042","display_name":"Turn (biochemistry)","level":2,"score":0.47524166107177734},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.34708401560783386},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2707034647464752},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19355043768882751},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.16519805788993835},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.158540278673172},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.1468101143836975},{"id":"https://openalex.org/C1276947","wikidata":"https://www.wikidata.org/wiki/Q333","display_name":"Astronomy","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000915","descriptor_name":"Antibody Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001792","descriptor_name":"Blood Platelets","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005340","descriptor_name":"Fibrinogen","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D005340","descriptor_name":"Fibrinogen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005340","descriptor_name":"Fibrinogen","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00159a008","doi":"https://doi.org/10.1021/bi00159a008","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00159a008","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:1420184","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1420184","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1452508983","display_name":"THROMBUS DETECTION USING FIBRIN-SPECIFIC ANTIBODIES","funder_award_id":"5r01hl028015-11","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G2826336473","display_name":null,"funder_award_id":"RR-03037","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G3230513115","display_name":null,"funder_award_id":"HL28015","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G7119219795","display_name":"ADHESIVE PROTEINS PLATELET RECEPTOR REGULATION","funder_award_id":"5r01hl045994-08","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G8227953362","display_name":null,"funder_award_id":"HL 45994","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1159817883","https://openalex.org/W1544623810","https://openalex.org/W1781023783","https://openalex.org/W1966593501","https://openalex.org/W1969632860","https://openalex.org/W1976654525","https://openalex.org/W1978195698","https://openalex.org/W1986130272","https://openalex.org/W1988529525","https://openalex.org/W1988835590","https://openalex.org/W1989469518","https://openalex.org/W1990320730","https://openalex.org/W1993159538","https://openalex.org/W1996911308","https://openalex.org/W2002744092","https://openalex.org/W2004896547","https://openalex.org/W2010528272","https://openalex.org/W2011629288","https://openalex.org/W2012499228","https://openalex.org/W2020782643","https://openalex.org/W2022248021","https://openalex.org/W2028243314","https://openalex.org/W2036728784","https://openalex.org/W2041108610","https://openalex.org/W2051549118","https://openalex.org/W2055126125","https://openalex.org/W2067127132","https://openalex.org/W2074770586","https://openalex.org/W2074977072","https://openalex.org/W2113403117","https://openalex.org/W2126719129","https://openalex.org/W2127439444","https://openalex.org/W2135846300","https://openalex.org/W2136701955","https://openalex.org/W2485617906","https://openalex.org/W2949250147"],"related_works":["https://openalex.org/W2589344718","https://openalex.org/W1993159538","https://openalex.org/W1974656135","https://openalex.org/W2012828249","https://openalex.org/W1556193518","https://openalex.org/W2031509562","https://openalex.org/W2044324500","https://openalex.org/W2031324615","https://openalex.org/W2774238720","https://openalex.org/W1986694471"],"abstract_inverted_index":{"The":[0,70,108],"interaction":[1],"between":[2],"fibrinogen":[3],"gamma-peptide":[4],"392-411,":[5,126],"LTIGEGQQHHLGGAKQAGDV,":[6],"and":[7,22,84,100,145,170,172,182],"monoclonal":[8],"antibody":[9,12,115,157,213],"4A5,":[10],"an":[11],"with":[13],"a":[14,29,48,163],"high":[15],"affinity":[16,109,129],"for":[17,19,31,74,114,187,204,212],"both":[18],"the":[20,38,45,82,85,104,111,128,131,140,180,192,201,205],"peptide":[21,40],"native":[23,124,141],"fibrinogen,":[24,169],"is":[25,68,117,134],"being":[26],"studied":[27],"as":[28],"model":[30],"peptide-antibody":[32],"interaction.":[33],"Two-dimensional":[34],"NMR":[35,101],"studies":[36,102],"of":[37,47,53,110,130,139,168,177,191],"free":[39],"at":[41],"pH":[42,61],"5.2":[43],"indicated":[44],"presence":[46],"significant":[49],"population,":[50],"about":[51],"60%,":[52],"type":[54],"II":[55],"beta-turn,":[56,83],"spanning":[57],"residues":[58,149],"Gln407-Asp410.":[59],"At":[60],"2.7,":[62],"little,":[63],"if":[64],"any,":[65],"turn":[66],"structure":[67],"present.":[69],"D-Ala409":[71,181],"analog,":[72,87],"which,":[73],"steric":[75,146],"reasons,":[76],"would":[77,89],"be":[78,91,152],"expected":[79,92],"to":[80,93],"preserve":[81],"L-Ala409":[86,183],"which":[88],"not":[90],"have":[94],"this":[95,171],"conformational":[96,144],"feature,":[97],"were":[98,185],"synthesized,":[99],"confirmed":[103],"respective":[105],"structural":[106],"predictions.":[107],"D-Ala":[112],"analog":[113,133],"4A5":[116],"even":[118],"greater":[119],"than":[120,136],"that":[121,138,200],"displayed":[122],"by":[123,156],"gamma":[125,160],"while":[127],"L-Ala":[132],"less":[135],"one-tenth":[137],"peptide.":[142],"Both":[143],"effects":[147],"involving":[148],"407-410":[150],"may":[151],"important":[153],"in":[154],"recognition":[155,202],"4A5.":[158,214],"Since":[159],"392-411":[161],"includes":[162],"platelet":[164,178,188,197,206],"receptor":[165,207],"binding":[166],"locus":[167],"related":[173],"peptides":[174],"are":[175],"inhibitors":[176],"aggregation,":[179],"analogs":[184,193],"tested":[186],"binding.":[189],"Neither":[190],"displays":[194],"any":[195],"measurable":[196],"binding,":[198],"indicating":[199],"requirements":[203],"differ":[208],"considerably":[209],"from":[210],"those":[211]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}