{"id":"https://openalex.org/W2017560258","doi":"https://doi.org/10.1007/s00299-004-0889-5","title":"A rapid and efficient transformation protocol for the grass Brachypodium distachyon","display_name":"A rapid and efficient transformation protocol for the grass Brachypodium distachyon","publication_year":2004,"publication_date":"2004-10-18","ids":{"openalex":"https://openalex.org/W2017560258","doi":"https://doi.org/10.1007/s00299-004-0889-5","mag":"2017560258","pmid":"https://pubmed.ncbi.nlm.nih.gov/15503032"},"language":"en","primary_location":{"id":"doi:10.1007/s00299-004-0889-5","doi":"https://doi.org/10.1007/s00299-004-0889-5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s00299-004-0889-5","pdf_url":null,"source":{"id":"https://openalex.org/S196783408","display_name":"Plant Cell Reports","issn_l":"0721-7714","issn":["0721-7714","1432-203X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Cell Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5114950401","display_name":"P. Christiansen","orcid":"https://orcid.org/0000-0002-6216-3644","observed_orcids":["https://orcid.org/0000-0002-6216-3644"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Pernille Christiansen","raw_affiliation_strings":["Ris\u00f8 National Laboratory, Frederiksborgvej 399, P.O. Box 49, 4000, Roskilde, Denmark. pernille.christiansen@risoe.dk","Ris\u00f8 National Laboratory, Roskilde, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ris\u00f8 National Laboratory, Frederiksborgvej 399, P.O. Box 49, 4000, Roskilde, Denmark. pernille.christiansen@risoe.dk","institution_ids":[]},{"raw_affiliation_string":"Ris\u00f8 National Laboratory, Roskilde, Denmark","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023338741","display_name":"Claus H. Andersen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142868","display_name":"DLF Trifolium (Denmark)","ror":"https://ror.org/04phr2832","country_code":"DK","type":"company","lineage":["https://openalex.org/I4210142868"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Claus Henrik Andersen","raw_affiliation_strings":["DLF-Trifolium A/S, Research Division, H\u00f8jerupvej 31, 4660, Store Heddinge, Denmark","DLF-Trifolium A/S, Research Division, Store Heddinge, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DLF-Trifolium A/S, Research Division, H\u00f8jerupvej 31, 4660, Store Heddinge, Denmark","institution_ids":["https://openalex.org/I4210142868"]},{"raw_affiliation_string":"DLF-Trifolium A/S, Research Division, Store Heddinge, Denmark","institution_ids":["https://openalex.org/I4210142868"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077477222","display_name":"Thomas Didion","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Thomas Didion","raw_affiliation_strings":["Ris\u00f8 National Laboratory, Frederiksborgvej 399, P.O. Box 49, 4000, Roskilde, Denmark","Ris\u00f8 National Laboratory, Roskilde, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ris\u00f8 National Laboratory, Frederiksborgvej 399, P.O. Box 49, 4000, Roskilde, Denmark","institution_ids":[]},{"raw_affiliation_string":"Ris\u00f8 National Laboratory, Roskilde, Denmark","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076665520","display_name":"Marianne Folling","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142868","display_name":"DLF Trifolium (Denmark)","ror":"https://ror.org/04phr2832","country_code":"DK","type":"company","lineage":["https://openalex.org/I4210142868"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Marianne Folling","raw_affiliation_strings":["DLF-Trifolium A/S, Research Division, H\u00f8jerupvej 31, 4660, Store Heddinge, Denmark","DLF-Trifolium A/S, Research Division, Store Heddinge, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DLF-Trifolium A/S, Research Division, H\u00f8jerupvej 31, 4660, Store Heddinge, Denmark","institution_ids":["https://openalex.org/I4210142868"]},{"raw_affiliation_string":"DLF-Trifolium A/S, Research Division, Store Heddinge, Denmark","institution_ids":["https://openalex.org/I4210142868"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5105193797","display_name":"Klaus Kristian Nielsen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142868","display_name":"DLF Trifolium (Denmark)","ror":"https://ror.org/04phr2832","country_code":"DK","type":"company","lineage":["https://openalex.org/I4210142868"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Klaus Kristian Nielsen","raw_affiliation_strings":["DLF-Trifolium A/S, Research Division, H\u00f8jerupvej 31, 4660, Store Heddinge, Denmark","DLF-Trifolium A/S, Research Division, Store Heddinge, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DLF-Trifolium A/S, Research Division, H\u00f8jerupvej 31, 4660, Store Heddinge, Denmark","institution_ids":["https://openalex.org/I4210142868"]},{"raw_affiliation_string":"DLF-Trifolium A/S, Research Division, Store Heddinge, Denmark","institution_ids":["https://openalex.org/I4210142868"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5114950401"],"corresponding_institution_ids":[],"apc_list":{"value":4590,"currency":"USD","value_usd":4590},"apc_paid":null,"fwci":4.95,"has_fulltext":false,"cited_by_count":82,"citation_normalized_percentile":{"value":0.94489793,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"23","issue":"10-11","first_page":"751","last_page":"758"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12828","display_name":"Plant Genetic and Mutation Studies","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brachypodium-distachyon","display_name":"Brachypodium distachyon","score":1.0},{"id":"https://openalex.org/keywords/particle-bombardment","display_name":"particle bombardment","score":0.9739999771118164},{"id":"https://openalex.org/keywords/agrobacterium-mediated-transformation","display_name":"Agrobacterium-mediated transformation","score":0.8220000267028809},{"id":"https://openalex.org/keywords/embryogenic-callus","display_name":"embryogenic callus","score":0.800000011920929},{"id":"https://openalex.org/keywords/immature-embryos","display_name":"immature embryos","score":0.7459999918937683},{"id":"https://openalex.org/keywords/transformation-efficiency","display_name":"transformation efficiency","score":0.6840000152587891},{"id":"https://openalex.org/keywords/gus-reporter","display_name":"GUS reporter","score":0.5699999928474426},{"id":"https://openalex.org/keywords/bar-gene","display_name":"bar gene","score":0.7829999923706055}],"concepts":[{"id":"https://openalex.org/C2777911459","wikidata":"https://www.wikidata.org/wiki/Q2352209","display_name":"Brachypodium distachyon","level":4,"score":0.9771690368652344},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9110493659973145},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.7504943609237671},{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.724174976348877},{"id":"https://openalex.org/C2778543611","wikidata":"https://www.wikidata.org/wiki/Q161426","display_name":"Oryza sativa","level":3,"score":0.7088727355003357},{"id":"https://openalex.org/C2777749068","wikidata":"https://www.wikidata.org/wiki/Q157770","display_name":"Brachypodium","level":4,"score":0.6790143251419067},{"id":"https://openalex.org/C143191323","wikidata":"https://www.wikidata.org/wiki/Q118406","display_name":"Ploidy","level":3,"score":0.6107094883918762},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.56612229347229},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.26779937744140625},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.25021958351135254},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.191898912191391}],"mesh":[{"descriptor_ui":"D000123","descriptor_name":"Acetyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004171","descriptor_name":"Diploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005966","descriptor_name":"Glucuronidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006109","descriptor_name":"Poaceae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011123","descriptor_name":"Polyploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014170","descriptor_name":"Transformation, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019470","descriptor_name":"Biolistics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D040582","descriptor_name":"Inheritance Patterns","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046509","descriptor_name":"Tissue Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1007/s00299-004-0889-5","doi":"https://doi.org/10.1007/s00299-004-0889-5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s00299-004-0889-5","pdf_url":null,"source":{"id":"https://openalex.org/S196783408","display_name":"Plant Cell Reports","issn_l":"0721-7714","issn":["0721-7714","1432-203X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Cell Reports","raw_type":"journal-article"},{"id":"pmid:15503032","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15503032","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant cell reports","raw_type":"Journal Article"},{"id":"pmh:oai:pure.atira.dk:publications/11876ef8-962e-4350-ba77-dd969cf46f6e","doi":null,"is_oa":false,"landing_page_url":"https://orbit.dtu.dk/en/publications/11876ef8-962e-4350-ba77-dd969cf46f6e","pdf_url":null,"source":{"id":"https://openalex.org/S4306400705","display_name":"Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I96673099","host_organization_name":"Technical University of Denmark","host_organization_lineage":["https://openalex.org/I96673099"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Christiansen , P , Andersen , C H , Didion , T , Folling , M & Nielsen , K K 2005 , ' A rapid and efficient transformation protocol for the grass Brachypodium distachyon ' , Plant Cell Rep. , vol. 23 , pp. 751-758 . https://doi.org/10.1007/s00299-004-0889-5","raw_type":"article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.9208999872207642}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W27792316","https://openalex.org/W90532991","https://openalex.org/W302119829","https://openalex.org/W1045220873","https://openalex.org/W1539503448","https://openalex.org/W1621355762","https://openalex.org/W1834586579","https://openalex.org/W1965859368","https://openalex.org/W1968718272","https://openalex.org/W1969553476","https://openalex.org/W1974824606","https://openalex.org/W1978862919","https://openalex.org/W1982572603","https://openalex.org/W1982766095","https://openalex.org/W1988757170","https://openalex.org/W1992776126","https://openalex.org/W1994420215","https://openalex.org/W2002395335","https://openalex.org/W2017084127","https://openalex.org/W2017843187","https://openalex.org/W2030281420","https://openalex.org/W2032850402","https://openalex.org/W2037512861","https://openalex.org/W2037593399","https://openalex.org/W2038811561","https://openalex.org/W2047594235","https://openalex.org/W2053788591","https://openalex.org/W2059185223","https://openalex.org/W2063784508","https://openalex.org/W2068504853","https://openalex.org/W2076583629","https://openalex.org/W2079461347","https://openalex.org/W2080917431","https://openalex.org/W2097149153","https://openalex.org/W2109384331","https://openalex.org/W2113076692","https://openalex.org/W2144634347","https://openalex.org/W2145410762","https://openalex.org/W2162524147","https://openalex.org/W2314225431","https://openalex.org/W2373468678","https://openalex.org/W4255006325","https://openalex.org/W6610739589"],"related_works":["https://openalex.org/W2403613707","https://openalex.org/W2922500309","https://openalex.org/W4255134714","https://openalex.org/W2017346866","https://openalex.org/W2066520111","https://openalex.org/W2766514068","https://openalex.org/W2094115707","https://openalex.org/W127330042","https://openalex.org/W2982295746","https://openalex.org/W2023349042"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":9},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":10}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}