{"id":"https://openalex.org/W2017520388","doi":"https://doi.org/10.1084/jem.176.6.1497","title":"Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease.","display_name":"Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease.","publication_year":1992,"publication_date":"1992-12-01","ids":{"openalex":"https://openalex.org/W2017520388","doi":"https://doi.org/10.1084/jem.176.6.1497","mag":"2017520388","pmid":"https://pubmed.ncbi.nlm.nih.gov/1460414","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2119445"},"language":"en","primary_location":{"id":"doi:10.1084/jem.176.6.1497","doi":"https://doi.org/10.1084/jem.176.6.1497","is_oa":true,"landing_page_url":"https://doi.org/10.1084/jem.176.6.1497","pdf_url":"https://rupress.org/jem/article-pdf/176/6/1497/1673929/1497.pdf","source":{"id":"https://openalex.org/S22947807","display_name":"The Journal of Experimental Medicine","issn_l":"0022-1007","issn":["0022-1007","1540-9538"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of experimental medicine","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2017520388"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://rupress.org/jem/article-pdf/176/6/1497/1673929/1497.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009306852","display_name":"Misao Matsushita","orcid":"https://orcid.org/0000-0002-0351-0562","observed_orcids":["https://orcid.org/0000-0002-0351-0562"]},"institutions":[{"id":"https://openalex.org/I130830085","display_name":"Fukushima Medical University","ror":"https://ror.org/012eh0r35","country_code":"JP","type":"education","lineage":["https://openalex.org/I130830085"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"M Matsushita","raw_affiliation_strings":["Department of Biochemistry, Fukushima Medical College, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Fukushima Medical College, Japan","institution_ids":["https://openalex.org/I130830085"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103076313","display_name":"Teizo Fujita","orcid":"https://orcid.org/0000-0001-9834-8985","observed_orcids":["https://orcid.org/0000-0001-9834-8985"]},"institutions":[{"id":"https://openalex.org/I130830085","display_name":"Fukushima Medical University","ror":"https://ror.org/012eh0r35","country_code":"JP","type":"education","lineage":["https://openalex.org/I130830085"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"T Fujita","raw_affiliation_strings":["Department of Biochemistry, Fukushima Medical College, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Fukushima Medical College, Japan","institution_ids":["https://openalex.org/I130830085"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I130830085"],"apc_list":null,"apc_paid":null,"fwci":4.22,"has_fulltext":true,"cited_by_count":648,"citation_normalized_percentile":{"value":0.95160026,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"176","issue":"6","first_page":"1497","last_page":"1502"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14245","display_name":"Biomarkers in Disease Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11917","display_name":"Coagulation, Bradykinin, Polyphosphates, and Angioedema","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mannose-binding-protein","display_name":"mannose-binding protein","score":0.9710000157356262},{"id":"https://openalex.org/keywords/classical-complement-pathway","display_name":"classical complement pathway","score":0.7860000133514404},{"id":"https://openalex.org/keywords/masp","display_name":"MASP","score":0.7720000147819519},{"id":"https://openalex.org/keywords/c4a","display_name":"C4A","score":0.3330000042915344},{"id":"https://openalex.org/keywords/c1r","display_name":"C1r","score":0.5590000152587891},{"id":"https://openalex.org/keywords/c1s","display_name":"C1s","score":0.7110000252723694},{"id":"https://openalex.org/keywords/c-type-lectin","display_name":"C-type lectin","score":0.828000009059906},{"id":"https://openalex.org/keywords/mannan","display_name":"mannan","score":0.5389999747276306},{"id":"https://openalex.org/keywords/complement-activation","display_name":"complement activation","score":0.515999972820282},{"id":"https://openalex.org/keywords/serine-protease","display_name":"serine protease","score":0.5740000009536743},{"id":"https://openalex.org/keywords/c1q","display_name":"C1q","score":0.34200000762939453}],"concepts":[{"id":"https://openalex.org/C189413060","wikidata":"https://www.wikidata.org/wiki/Q415443","display_name":"Mannan-binding lectin","level":3,"score":0.7496920824050903},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.6931795477867126},{"id":"https://openalex.org/C82553315","wikidata":"https://www.wikidata.org/wiki/Q14905312","display_name":"MASP1","level":5,"score":0.6923328638076782},{"id":"https://openalex.org/C111684460","wikidata":"https://www.wikidata.org/wiki/Q334848","display_name":"Complement system","level":3,"score":0.6389050483703613},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6317094564437866},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.6012202501296997},{"id":"https://openalex.org/C171279029","wikidata":"https://www.wikidata.org/wiki/Q5128320","display_name":"Classical complement pathway","level":4,"score":0.5934123396873474},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.5845102071762085},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.5417753458023071},{"id":"https://openalex.org/C2775887612","wikidata":"https://www.wikidata.org/wiki/Q10809118","display_name":"Mannose","level":2,"score":0.5293247103691101},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4937001168727875},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.4606265127658844},{"id":"https://openalex.org/C203880312","wikidata":"https://www.wikidata.org/wiki/Q14864145","display_name":"Lectin pathway","level":5,"score":0.4435100555419922},{"id":"https://openalex.org/C124594742","wikidata":"https://www.wikidata.org/wiki/Q5446528","display_name":"Ficolin","level":4,"score":0.42134860157966614},{"id":"https://openalex.org/C2779719659","wikidata":"https://www.wikidata.org/wiki/Q24577942","display_name":"Mannan","level":3,"score":0.4112260937690735},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3594188094139099},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.22488874197006226},{"id":"https://openalex.org/C2780758401","wikidata":"https://www.wikidata.org/wiki/Q408693","display_name":"Lectin","level":2,"score":0.17328065633773804},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.11167168617248535},{"id":"https://openalex.org/C100817775","wikidata":"https://www.wikidata.org/wiki/Q134219","display_name":"Polysaccharide","level":2,"score":0.07892373204231262},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003171","descriptor_name":"Complement Pathway, Classical","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D003173","descriptor_name":"Complement C1s","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D003173","descriptor_name":"Complement C1s","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003175","descriptor_name":"Complement C2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003181","descriptor_name":"Complement C4","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008358","descriptor_name":"Mannose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D037241","descriptor_name":"Mannose-Binding Lectins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1084/jem.176.6.1497","doi":"https://doi.org/10.1084/jem.176.6.1497","is_oa":true,"landing_page_url":"https://doi.org/10.1084/jem.176.6.1497","pdf_url":"https://rupress.org/jem/article-pdf/176/6/1497/1673929/1497.pdf","source":{"id":"https://openalex.org/S22947807","display_name":"The Journal of Experimental Medicine","issn_l":"0022-1007","issn":["0022-1007","1540-9538"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of experimental medicine","raw_type":"journal-article"},{"id":"pmid:1460414","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1460414","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of experimental medicine","raw_type":"Comparative Study"},{"id":"pmh:oai:pubmedcentral.nih.gov:2119445","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2119445/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Exp Med","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1084/jem.176.6.1497","doi":"https://doi.org/10.1084/jem.176.6.1497","is_oa":true,"landing_page_url":"https://doi.org/10.1084/jem.176.6.1497","pdf_url":"https://rupress.org/jem/article-pdf/176/6/1497/1673929/1497.pdf","source":{"id":"https://openalex.org/S22947807","display_name":"The Journal of Experimental Medicine","issn_l":"0022-1007","issn":["0022-1007","1540-9538"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of experimental medicine","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320325453","display_name":"Nagoya City University","ror":"https://ror.org/04wn7wc95"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2017520388.pdf","grobid_xml":"https://content.openalex.org/works/W2017520388.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W40099405","https://openalex.org/W1522572101","https://openalex.org/W1528847106","https://openalex.org/W1557110838","https://openalex.org/W1573617710","https://openalex.org/W1592289975","https://openalex.org/W1629182995","https://openalex.org/W1889222490","https://openalex.org/W1946252599","https://openalex.org/W1950537519","https://openalex.org/W1966696304","https://openalex.org/W1972601218","https://openalex.org/W1984469505","https://openalex.org/W2004998426","https://openalex.org/W2013946123","https://openalex.org/W2018289835","https://openalex.org/W2024822222","https://openalex.org/W2028154775","https://openalex.org/W2031384372","https://openalex.org/W2033631197","https://openalex.org/W2047233465","https://openalex.org/W2060469959","https://openalex.org/W2070894409","https://openalex.org/W2073598242","https://openalex.org/W2083771700","https://openalex.org/W2100837269","https://openalex.org/W2119799948","https://openalex.org/W2136212260","https://openalex.org/W2153539427","https://openalex.org/W2158620829","https://openalex.org/W2171423889","https://openalex.org/W2273370733"],"related_works":["https://openalex.org/W1993757698","https://openalex.org/W2046577485","https://openalex.org/W2074497479","https://openalex.org/W2061147238","https://openalex.org/W1522572101","https://openalex.org/W1557110838","https://openalex.org/W2067957598","https://openalex.org/W1991163875","https://openalex.org/W2585732487"],"abstract_inverted_index":{"Serum":[0],"mannose-binding":[1],"protein":[2,191],"(MBP)":[3],"is":[4,141,188],"a":[5,67,189,204],"C-type":[6],"lectin":[7],"that":[8,154,186],"binds":[9],"to":[10,113,214],"terminal":[11],"mannose":[12],"and":[13,22,56,103,124,151,162,174],"N-acetylglucosamine":[14],"moieties":[15],"present":[16,30],"on":[17,53,91,143,165,168],"surfaces":[18],"of":[19,61,96,109,120,156,170,180],"certain":[20],"pathogens":[21],"activates":[23],"the":[24,29,34,37,59,94,144,157,181,211,216],"classical":[25,217],"complement":[26,218],"pathway.":[27,219],"In":[28],"study,":[31],"we":[32,184],"describe":[33],"mechanism":[35,206],"underlying":[36],"activation":[38],"triggered":[39],"by":[40,49,73,89,131,207],"MBP.":[41],"The":[42,76,106,134],"human":[43],"serum":[44],"MBP":[45,88,152,209],"fraction":[46,65],"was":[47,85,111],"obtained":[48],"sequential":[50],"affinity":[51],"chromatography":[52],"mannan-Sepharose,":[54],"anti-IgM-Sepharose":[55],"anti-MBP-Sepharose":[57,92],"in":[58,93,201],"presence":[60,95],"calcium":[62],"ions.":[63],"This":[64],"contained":[66],"C1s-like":[68,77],"serine":[69,78,82,135],"protease":[70,83,139],"as":[71],"assessed":[72],"C4":[74],"consumption.":[75],"protease,":[79],"designated":[80],"MBP-associated":[81],"(MASP),":[84],"separated":[86],"from":[87,193],"rechromatography":[90],"ethylenediaminetetraacetic":[97],"acid.":[98],"MASP":[99,110,150,187],"exhibited":[100],"both":[101],"C4-":[102,161],"C2-consuming":[104],"activities.":[105],"molecular":[107,171],"mass":[108],"estimated":[112],"be":[114],"83":[115],"kD":[116],"with":[117,203],"two":[118,158],"polypeptides":[119],"heavy":[121],"(66":[122],"kD)":[123,128],"light":[125],"(L)":[126],"(31":[127],"chains":[129],"linked":[130],"disulfide":[132],"bonds.":[133],"residue":[136],"responsible":[137],"for":[138],"activity":[140],"located":[142],"L":[145,182],"chain.":[146],"Reconstitution":[147],"experiments":[148],"using":[149],"revealed":[153],"combination":[155],"components":[159],"restores":[160],"C2-activating":[163],"capacity":[164],"mannan.":[166],"Based":[167],"analyses":[169],"size,":[172],"antigenicity,":[173],"11":[175],"NH2-terminal":[176],"amino":[177],"acid":[178],"sequences":[179],"chain,":[183],"conclude":[185],"novel":[190],"different":[192],"C1r":[194],"or":[195],"C1s.":[196],"Our":[197],"findings":[198],"are":[199],"not":[200],"accord":[202],"proposed":[205],"which":[208],"utilizes":[210],"C1r2-C1s2":[212],"complex":[213],"initiate":[215]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":15},{"year":2022,"cited_by_count":12},{"year":2021,"cited_by_count":14},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":10},{"year":2017,"cited_by_count":10},{"year":2016,"cited_by_count":14},{"year":2015,"cited_by_count":16},{"year":2014,"cited_by_count":10},{"year":2013,"cited_by_count":17},{"year":2012,"cited_by_count":22}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}