{"id":"https://openalex.org/W2017519284","doi":"https://doi.org/10.1016/0003-2697(77)90289-5","title":"Specific protein-binding reactions monitored by enzymatic hydrolysis of ligand-fluorescent dye conjugates","display_name":"Specific protein-binding reactions monitored by enzymatic hydrolysis of ligand-fluorescent dye conjugates","publication_year":1977,"publication_date":"1977-01-01","ids":{"openalex":"https://openalex.org/W2017519284","doi":"https://doi.org/10.1016/0003-2697(77)90289-5","mag":"2017519284","pmid":"https://pubmed.ncbi.nlm.nih.gov/12682"},"language":"en","primary_location":{"id":"doi:10.1016/0003-2697(77)90289-5","doi":"https://doi.org/10.1016/0003-2697(77)90289-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(77)90289-5","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040121120","display_name":"John F. Burd","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J.F. Burd","raw_affiliation_strings":["Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]},{"raw_affiliation_string":"Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074654930","display_name":"Robert J. Carrico","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R.J. Carrico","raw_affiliation_strings":["Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]},{"raw_affiliation_string":"Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059310274","display_name":"Marion C. Fetter","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M.C. Fetter","raw_affiliation_strings":["Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]},{"raw_affiliation_string":"Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082928996","display_name":"Robert T. Buckler","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R.T. Buckler","raw_affiliation_strings":["Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]},{"raw_affiliation_string":"Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002070229","display_name":"R D Johnson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R.D. Johnson","raw_affiliation_strings":["Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]},{"raw_affiliation_string":"Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074199875","display_name":"Robert C. Boguslaski","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R.C. Boguslaski","raw_affiliation_strings":["Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]},{"raw_affiliation_string":"Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091534077","display_name":"James E. Christner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J.E. Christner","raw_affiliation_strings":["Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames Research Laboratory, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]},{"raw_affiliation_string":"Chemistry Department, Corporate Research, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":1.6904,"has_fulltext":false,"cited_by_count":51,"citation_normalized_percentile":{"value":0.82659823,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"77","issue":"1","first_page":"56","last_page":"67"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-ligand-binding","display_name":"protein-ligand binding","score":0.7860000133514404},{"id":"https://openalex.org/keywords/enzymatic-hydrolysis","display_name":"enzymatic hydrolysis","score":0.847000002861023},{"id":"https://openalex.org/keywords/umbelliferone","display_name":"umbelliferone","score":0.7680000066757202},{"id":"https://openalex.org/keywords/fluorescein","display_name":"fluorescein","score":0.8679999709129333},{"id":"https://openalex.org/keywords/biotin","display_name":"biotin","score":0.5690000057220459},{"id":"https://openalex.org/keywords/2-4-dinitrophenyl-derivatives","display_name":"2,4-dinitrophenyl derivatives","score":0.9120000004768372},{"id":"https://openalex.org/keywords/competitive-binding","display_name":"competitive binding","score":0.6140000224113464},{"id":"https://openalex.org/keywords/antibody-purification","display_name":"antibody purification","score":0.8569999933242798},{"id":"https://openalex.org/keywords/avidin","display_name":"avidin","score":0.6700000166893005},{"id":"https://openalex.org/keywords/ester-linkage","display_name":"ester linkage","score":0.46299999952316284}],"concepts":[{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.8986319899559021},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8914660811424255},{"id":"https://openalex.org/C112613896","wikidata":"https://www.wikidata.org/wiki/Q898370","display_name":"Conjugated system","level":3,"score":0.7813166379928589},{"id":"https://openalex.org/C2777261171","wikidata":"https://www.wikidata.org/wiki/Q418750","display_name":"Esterase","level":3,"score":0.6651102304458618},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6507210731506348},{"id":"https://openalex.org/C2779020807","wikidata":"https://www.wikidata.org/wiki/Q416196","display_name":"Umbelliferone","level":3,"score":0.6234207153320312},{"id":"https://openalex.org/C2778177356","wikidata":"https://www.wikidata.org/wiki/Q19903955","display_name":"Dinitrophenyl","level":3,"score":0.6161595582962036},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.6160783767700195},{"id":"https://openalex.org/C2778015030","wikidata":"https://www.wikidata.org/wiki/Q410922","display_name":"Fluorescein","level":3,"score":0.6109427213668823},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.5504915118217468},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.534051775932312},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.5098215937614441},{"id":"https://openalex.org/C176406525","wikidata":"https://www.wikidata.org/wiki/Q24775166","display_name":"Avidin","level":3,"score":0.4605942964553833},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.45560047030448914},{"id":"https://openalex.org/C2778204606","wikidata":"https://www.wikidata.org/wiki/Q181354","display_name":"Biotin","level":2,"score":0.39357370138168335},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3886583745479584},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.32349157333374023},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31322795152664185},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.26620152592658997},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.10329988598823547},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.09898307919502258},{"id":"https://openalex.org/C2781338262","wikidata":"https://www.wikidata.org/wiki/Q111812","display_name":"Coumarin","level":2,"score":0.07496723532676697},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001360","descriptor_name":"Avidin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001710","descriptor_name":"Biotin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004140","descriptor_name":"Dinitrophenols","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005452","descriptor_name":"Fluoresceins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011869","descriptor_name":"Radioligand Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0003-2697(77)90289-5","doi":"https://doi.org/10.1016/0003-2697(77)90289-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(77)90289-5","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:12682","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12682","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W150919610","https://openalex.org/W1969763882","https://openalex.org/W1996514181","https://openalex.org/W1998510552","https://openalex.org/W2086629831","https://openalex.org/W2258009642","https://openalex.org/W2260584230","https://openalex.org/W2317643037","https://openalex.org/W4247023439"],"related_works":["https://openalex.org/W2086629831","https://openalex.org/W2031765410","https://openalex.org/W1969763882","https://openalex.org/W2066687776","https://openalex.org/W2065902634","https://openalex.org/W2037576778","https://openalex.org/W1999295051","https://openalex.org/W1492706981","https://openalex.org/W1970856637","https://openalex.org/W2022622012"],"abstract_inverted_index":null,"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}