{"id":"https://openalex.org/W2017488295","doi":"https://doi.org/10.1016/0003-2697(70)90387-8","title":"Improved method for characterization of bacterial dehydrogenases using acrylamide gel disc electrophoresis","display_name":"Improved method for characterization of bacterial dehydrogenases using acrylamide gel disc electrophoresis","publication_year":1970,"publication_date":"1970-08-01","ids":{"openalex":"https://openalex.org/W2017488295","doi":"https://doi.org/10.1016/0003-2697(70)90387-8","mag":"2017488295","pmid":"https://pubmed.ncbi.nlm.nih.gov/4916450"},"language":"en","primary_location":{"id":"doi:10.1016/0003-2697(70)90387-8","doi":"https://doi.org/10.1016/0003-2697(70)90387-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(70)90387-8","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110872721","display_name":"G. W. Skyring","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1331897569","display_name":"Agriculture and Agri-Food Canada","ror":"https://ror.org/051dzs374","country_code":"CA","type":"government","lineage":["https://openalex.org/I1331897569","https://openalex.org/I2802286613"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"G.W. Skyring","raw_affiliation_strings":["Cell Biology Research Institute, Research Branch, Canada Department of Agriculture, Ottawa, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell Biology Research Institute, Research Branch, Canada Department of Agriculture, Ottawa, Ontario, Canada","institution_ids":["https://openalex.org/I1331897569"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022881733","display_name":"Richard W. Miller","orcid":"https://orcid.org/0000-0002-4409-1173","observed_orcids":["https://orcid.org/0000-0002-4409-1173"]},"institutions":[{"id":"https://openalex.org/I1331897569","display_name":"Agriculture and Agri-Food Canada","ror":"https://ror.org/051dzs374","country_code":"CA","type":"government","lineage":["https://openalex.org/I1331897569","https://openalex.org/I2802286613"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"R.W. Miller","raw_affiliation_strings":["Cell Biology Research Institute, Research Branch, Canada Department of Agriculture, Ottawa, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell Biology Research Institute, Research Branch, Canada Department of Agriculture, Ottawa, Ontario, Canada","institution_ids":["https://openalex.org/I1331897569"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005990971","display_name":"Vesna Purkayastha","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1331897569","display_name":"Agriculture and Agri-Food Canada","ror":"https://ror.org/051dzs374","country_code":"CA","type":"government","lineage":["https://openalex.org/I1331897569","https://openalex.org/I2802286613"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"V. Purkayastha","raw_affiliation_strings":["Cell Biology Research Institute, Research Branch, Canada Department of Agriculture, Ottawa, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell Biology Research Institute, Research Branch, Canada Department of Agriculture, Ottawa, Ontario, Canada","institution_ids":["https://openalex.org/I1331897569"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1331897569"],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":0.6612,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.62799263,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"36","issue":"2","first_page":"511","last_page":"520"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.5810999870300293,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.5810999870300293,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.12919999659061432,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.09759999811649323,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactate-dehydrogenase","display_name":"lactate dehydrogenase","score":0.8230000138282776},{"id":"https://openalex.org/keywords/succinate-dehydrogenase","display_name":"succinate dehydrogenase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/malic-dehydrogenase","display_name":"malic dehydrogenase","score":1.0},{"id":"https://openalex.org/keywords/glutamate-dehydrogenase","display_name":"glutamate dehydrogenase","score":0.9779999852180481},{"id":"https://openalex.org/keywords/dihydroorotate-dehydrogenase","display_name":"dihydroorotate dehydrogenase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/triton-x-100-solubilization","display_name":"Triton X-100 solubilization","score":0.949999988079071},{"id":"https://openalex.org/keywords/manganese-chloride","display_name":"manganese chloride","score":0.6129999756813049},{"id":"https://openalex.org/keywords/potassium-cyanide","display_name":"potassium cyanide","score":0.5770000219345093}],"concepts":[{"id":"https://openalex.org/C2779865431","wikidata":"https://www.wikidata.org/wiki/Q1150909","display_name":"Dihydroorotate dehydrogenase","level":3,"score":0.6953386664390564},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6790406703948975},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.6472993493080139},{"id":"https://openalex.org/C132901563","wikidata":"https://www.wikidata.org/wiki/Q412007","display_name":"Malate dehydrogenase","level":3,"score":0.6077902317047119},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5719802379608154},{"id":"https://openalex.org/C2777318727","wikidata":"https://www.wikidata.org/wiki/Q409464","display_name":"Lactate dehydrogenase","level":3,"score":0.5471141338348389},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5324844717979431},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.5246948599815369},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5176149606704712},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.5001015663146973},{"id":"https://openalex.org/C2781330172","wikidata":"https://www.wikidata.org/wiki/Q412837","display_name":"Succinate dehydrogenase","level":3,"score":0.4786323606967926},{"id":"https://openalex.org/C2777202666","wikidata":"https://www.wikidata.org/wiki/Q417383","display_name":"Glutamate dehydrogenase","level":4,"score":0.4142776131629944},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C61174792","wikidata":"https://www.wikidata.org/wiki/Q420510","display_name":"Glutamate receptor","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001173","descriptor_name":"Arthrobacter","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004589","descriptor_name":"Electrophoresis, Disc","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D005954","descriptor_name":"Glucosephosphate Dehydrogenase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005969","descriptor_name":"Glutamate Dehydrogenase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D007521","descriptor_name":"Isocitrate Dehydrogenase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008291","descriptor_name":"Malate Dehydrogenase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D010734","descriptor_name":"Phosphogluconate Dehydrogenase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012995","descriptor_name":"Solubility","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013385","descriptor_name":"Succinate Dehydrogenase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013501","descriptor_name":"Surface-Active Agents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013778","descriptor_name":"Tetrazolium Salts","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0003-2697(70)90387-8","doi":"https://doi.org/10.1016/0003-2697(70)90387-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(70)90387-8","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:4916450","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4916450","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W121198892","https://openalex.org/W1535840072","https://openalex.org/W1564950588","https://openalex.org/W1585892700","https://openalex.org/W1601347703","https://openalex.org/W2018939295","https://openalex.org/W2093718624","https://openalex.org/W2100665160","https://openalex.org/W2155471788","https://openalex.org/W2156024135"],"related_works":["https://openalex.org/W2022034562","https://openalex.org/W2002299445","https://openalex.org/W3133542897","https://openalex.org/W1974031557","https://openalex.org/W2058761673","https://openalex.org/W2037072055","https://openalex.org/W2411301301","https://openalex.org/W2070286543","https://openalex.org/W2081918072","https://openalex.org/W613894164"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}