{"id":"https://openalex.org/W2017423159","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb05694.x","title":"Immunochemical Studies of Factor V of Bovine Platelets","display_name":"Immunochemical Studies of Factor V of Bovine Platelets","publication_year":1981,"publication_date":"1981-11-01","ids":{"openalex":"https://openalex.org/W2017423159","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb05694.x","mag":"2017423159","pmid":"https://pubmed.ncbi.nlm.nih.gov/7032911"},"language":"en","primary_location":{"id":"doi:10.1111/j.1432-1033.1981.tb05694.x","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb05694.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1981.tb05694.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061196254","display_name":"Thomas Roy Ittyerah","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. Roy ITTYERAH","raw_affiliation_strings":["Thrombosis Research Center, Temple University Health Science Centre, 3400 North-Broad Street, Philadelphia, Pennsylvania, USA 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thrombosis Research Center, Temple University Health Science Centre, 3400 North-Broad Street, Philadelphia, Pennsylvania, USA 19140","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066338272","display_name":"Razia Rawala","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Razia RAWALA","raw_affiliation_strings":["Thrombosis Research Center, Temple University Health Science Centre, 3400 North-Broad Street, Philadelphia, Pennsylvania, USA 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thrombosis Research Center, Temple University Health Science Centre, 3400 North-Broad Street, Philadelphia, Pennsylvania, USA 19140","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5104652394","display_name":"Robert W. Colman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert W. COLMAN","raw_affiliation_strings":["Thrombosis Research Center, Temple University Health Science Centre, 3400 North-Broad Street, Philadelphia, Pennsylvania, USA 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thrombosis Research Center, Temple University Health Science Centre, 3400 North-Broad Street, Philadelphia, Pennsylvania, USA 19140","institution_ids":["https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I84392919"],"apc_list":null,"apc_paid":null,"fwci":1.3065,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.79054579,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"120","issue":"2","first_page":"235","last_page":"241"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.5874000191688538,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.5874000191688538,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.3935999870300293,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":0.008999999612569809,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bovine-platelets","display_name":"bovine platelets","score":0.8709999918937683},{"id":"https://openalex.org/keywords/antigenic-identity","display_name":"antigenic identity","score":0.6940000057220459},{"id":"https://openalex.org/keywords/thrombin-activation","display_name":"thrombin activation","score":0.871999979019165},{"id":"https://openalex.org/keywords/alpha-granules","display_name":"alpha-granules","score":0.8840000033378601},{"id":"https://openalex.org/keywords/proteolytic-modification","display_name":"proteolytic modification","score":0.6840000152587891},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.7200000286102295},{"id":"https://openalex.org/keywords/subcellular-localization","display_name":"subcellular localization","score":0.6819999814033508},{"id":"https://openalex.org/keywords/coagulant-activity","display_name":"coagulant activity","score":0.703000009059906}],"concepts":[{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.6950575709342957},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6621392369270325},{"id":"https://openalex.org/C2779390175","wikidata":"https://www.wikidata.org/wiki/Q855786","display_name":"Immunoelectrophoresis","level":3,"score":0.6534280180931091},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.6389576196670532},{"id":"https://openalex.org/C2777292125","wikidata":"https://www.wikidata.org/wiki/Q409166","display_name":"Thrombin","level":3,"score":0.6058395504951477},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.46068859100341797},{"id":"https://openalex.org/C2776046644","wikidata":"https://www.wikidata.org/wiki/Q422831","display_name":"Factor V","level":3,"score":0.4466351866722107},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.4439469575881958},{"id":"https://openalex.org/C2780930249","wikidata":"https://www.wikidata.org/wiki/Q412850","display_name":"Factor VII","level":3,"score":0.4219111502170563},{"id":"https://openalex.org/C186738567","wikidata":"https://www.wikidata.org/wiki/Q412841","display_name":"Tissue factor","level":3,"score":0.4209808111190796},{"id":"https://openalex.org/C196955773","wikidata":"https://www.wikidata.org/wiki/Q256632","display_name":"Immunodiffusion","level":3,"score":0.41921716928482056},{"id":"https://openalex.org/C159973064","wikidata":"https://www.wikidata.org/wiki/Q3740205","display_name":"Platelet factor 4","level":3,"score":0.41034501791000366},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.40517565608024597},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.3506595492362976},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1688590943813324},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1679610311985016},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1666449010372162},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.1382446587085724},{"id":"https://openalex.org/C2778382381","wikidata":"https://www.wikidata.org/wiki/Q179217","display_name":"Coagulation","level":2,"score":0.12168645858764648},{"id":"https://openalex.org/C2777557582","wikidata":"https://www.wikidata.org/wiki/Q190016","display_name":"Heparin","level":2,"score":0.08072182536125183},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.05861985683441162},{"id":"https://openalex.org/C2780868729","wikidata":"https://www.wikidata.org/wiki/Q261327","display_name":"Thrombosis","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001792","descriptor_name":"Blood Platelets","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003094","descriptor_name":"Collagen","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005165","descriptor_name":"Factor V","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D005165","descriptor_name":"Factor V","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007120","descriptor_name":"Immunochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D010949","descriptor_name":"Plasma","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010949","descriptor_name":"Plasma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013917","descriptor_name":"Thrombin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1432-1033.1981.tb05694.x","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb05694.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1981.tb05694.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:7032911","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7032911","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W38611617","https://openalex.org/W235933110","https://openalex.org/W1519085965","https://openalex.org/W1527081709","https://openalex.org/W1531550096","https://openalex.org/W1534016881","https://openalex.org/W1554490272","https://openalex.org/W1554919783","https://openalex.org/W1561534093","https://openalex.org/W1575000195","https://openalex.org/W1599055297","https://openalex.org/W1604706922","https://openalex.org/W1625660792","https://openalex.org/W1955011237","https://openalex.org/W1975183263","https://openalex.org/W1980796560","https://openalex.org/W1985152715","https://openalex.org/W1990986783","https://openalex.org/W1994419975","https://openalex.org/W2008937610","https://openalex.org/W2018377262","https://openalex.org/W2028414268","https://openalex.org/W2048886671","https://openalex.org/W2050281169","https://openalex.org/W2052113589","https://openalex.org/W2285458529","https://openalex.org/W2287738335","https://openalex.org/W2334613226","https://openalex.org/W2399096342","https://openalex.org/W2399984400","https://openalex.org/W2461138026","https://openalex.org/W4247621043"],"related_works":["https://openalex.org/W2945433919","https://openalex.org/W2945583704","https://openalex.org/W38611617","https://openalex.org/W1968049480","https://openalex.org/W2104431307","https://openalex.org/W2118266481","https://openalex.org/W1563567604","https://openalex.org/W1994419975","https://openalex.org/W1561534093","https://openalex.org/W2888971439"],"abstract_inverted_index":{"Immunochemical":[0],"techniques":[1],"were":[2],"employed":[3],"to":[4,34,58,79,84,115,141,157,210,239],"investigate":[5],"the":[6,72,82,94,99,116,165,179,184,232,251],"molecular":[7,193,262],"properties":[8],"of":[9,40,74,101,145,155,168,192,220,253],"bovine":[10],"platelet":[11,23,28,59,63,110,159,200,225],"factor":[12,19,24,29,36,43,49,60,64,86,111,122,146,160,169,201,226,236,248],"V.":[13,37,61],"An":[14],"antiserum":[15,83],"prepared":[16],"against":[17,55,81],"purified":[18,48],"V":[20,30,44,50,65,112,123,147,161,170,202,215,227,237,249],"rapidly":[21],"inactivated":[22],"V,":[25,87],"indicating":[26],"that":[27,178,224],"is":[31,181,228],"antigenically":[32],"related":[33],"plasma":[35,46,85,149,247],"A":[38,189],"reaction":[39],"identity":[41],"between":[42],"in":[45,71,148,183,231],"and":[47,77,137,172,208,218,259],"was":[51,66,96,132,197,206],"documented":[52],"by":[53,106,125,134,175,204,250],"immunodiffusion":[54],"an":[56],"antibody":[57],"When":[62],"extracted":[67,128],"with":[68,129],"Triton":[69,130],"X-100":[70,131],"presence":[73],"protease":[75,102],"inhibitors":[76],"subjected":[78,209],"immunoelectrophoresis":[80,219],"a":[88,142],"single":[89,190],"antigenic":[90],"component":[91,191],"migrating":[92],"toward":[93],"anode":[95],"observed.":[97],"In":[98],"absence":[100],"inhibitors,":[103],"following":[104],"release":[105],"collagen":[107,126,176,205],"or":[108,127],"solubilization,":[109],"appeared":[113],"close":[114,166],"origin":[117],"suggesting":[118],"proteolytic":[119],"alteration.":[120],"Platelet":[121,235],"released":[124,174,203],"activated":[133],"thrombin":[135,156],"7.4-fold":[136],"4.5-fold":[138],"respectively,":[139],"compared":[140],"17-fold":[143],"activation":[144],"under":[150],"identical":[151,245,261],"conditions.":[152],"The":[153],"ability":[154],"activate":[158],"as":[162,164],"well":[163],"correspondence":[167],"activity":[171],"antigen":[173],"indicates":[177],"molecule":[180],"largely":[182],"unactivated":[185],"form":[186],"after":[187],"release.":[188],"weight":[194],"270":[195],"000":[196],"seen":[198],"when":[199],"immunoprecipitated":[207],"dodecylsulphate":[211],"gel":[212],"electrophoresis.":[213],"Factor":[214],"coagulant":[216],"assays":[217],"subcellular":[221],"fractions":[222],"showed":[223],"localized":[229],"primarily":[230],"alpha":[233],"granules.":[234],"appears":[238],"be":[240],"similar":[241,256],"to,":[242],"if":[243],"not":[244],"with,":[246],"criteria":[252],"immunologic":[254],"identity,":[255],"electrophoretic":[257],"mobility":[258],"virtually":[260],"weights.":[263]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}