{"id":"https://openalex.org/W2017281695","doi":"https://doi.org/10.4155/bio.11.58","title":"Comparison of UHPLC and HPLC Methods for The Assay of Prostanoids: \u201cAre The Methods Equivalent in Terms of Accuracy and Precision?\u201d","display_name":"Comparison of UHPLC and HPLC Methods for The Assay of Prostanoids: \u201cAre The Methods Equivalent in Terms of Accuracy and Precision?\u201d","publication_year":2011,"publication_date":"2011-04-01","ids":{"openalex":"https://openalex.org/W2017281695","doi":"https://doi.org/10.4155/bio.11.58","mag":"2017281695","pmid":"https://pubmed.ncbi.nlm.nih.gov/21510759"},"language":"en","primary_location":{"id":"doi:10.4155/bio.11.58","doi":"https://doi.org/10.4155/bio.11.58","is_oa":false,"landing_page_url":"https://doi.org/10.4155/bio.11.58","pdf_url":null,"source":{"id":"https://openalex.org/S159955403","display_name":"Bioanalysis","issn_l":"1757-6180","issn":["1757-6180","1757-6199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108476688","display_name":"Deepti Varma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Deepti Varma","raw_affiliation_strings":["Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015536602","display_name":"Satya Ganti","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Satya Ganti","raw_affiliation_strings":["Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091272697","display_name":"Tiffany Gilles","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tiffany Gilles","raw_affiliation_strings":["Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Melissa Diaz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melissa Diaz","raw_affiliation_strings":["Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071563721","display_name":"Teairra Thompson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I56505888","display_name":"Community College of Philadelphia","ror":"https://ror.org/00hm77b03","country_code":"US","type":"education","lineage":["https://openalex.org/I56505888"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Teairra Thompson","raw_affiliation_strings":["Community College of Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Community College of Philadelphia, PA, USA","institution_ids":["https://openalex.org/I56505888"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087657882","display_name":"Judith C. Stull","orcid":"https://orcid.org/0000-0002-2577-8551","observed_orcids":["https://orcid.org/0000-0002-2577-8551"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Judith Stull","raw_affiliation_strings":["Temple University, Institute for Schools &amp; Society, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Temple University, Institute for Schools &amp; Society, PA, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103447541","display_name":"Susan A. Jansen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan A Jansen","raw_affiliation_strings":["Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA19122, USA","institution_ids":["https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3133,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.60991745,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"3","issue":"8","first_page":"853","last_page":"862"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11890","display_name":"Scientific Measurement and Uncertainty Evaluation","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1804","display_name":"Statistics, Probability and Uncertainty"},"field":{"id":"https://openalex.org/fields/18","display_name":"Decision Sciences"},"domain":{"id":"https://openalex.org/domains/2","display_name":"Social Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/prostanoids","display_name":"prostanoids","score":0.8989999890327454},{"id":"https://openalex.org/keywords/ultra-high-performance-liquid-chromatography","display_name":"UPLC","score":0.7760000228881836},{"id":"https://openalex.org/keywords/high-performance-liquid-chromatography","display_name":"HPLC","score":0.8989999890327454},{"id":"https://openalex.org/keywords/method-comparison","display_name":"method comparison","score":0.7599999904632568},{"id":"https://openalex.org/keywords/method-equivalence","display_name":"method equivalence","score":0.8489999771118164},{"id":"https://openalex.org/keywords/method-bias","display_name":"methodological bias","score":0.5630000233650208},{"id":"https://openalex.org/keywords/precision","display_name":"precision","score":0.6850000023841858},{"id":"https://openalex.org/keywords/8-isoprostane","display_name":"8-isoprostane","score":0.859000027179718},{"id":"https://openalex.org/keywords/prostaglandins","display_name":"prostaglandins","score":0.7870000004768372},{"id":"https://openalex.org/keywords/15-deoxy-delta12-14-pgj2","display_name":"15-deoxy-delta-12,14PGJ2","score":0.8299999833106995},{"id":"https://openalex.org/keywords/6-keto-pgf1alpha","display_name":"6-keto-PGF1 alpha","score":0.8460000157356262},{"id":"https://openalex.org/keywords/deming-regression","display_name":"Deming regression","score":0.7369999885559082},{"id":"https://openalex.org/keywords/bland-altman-analysis","display_name":"Bland-Altman analysis","score":0.9660000205039978},{"id":"https://openalex.org/keywords/commutability","display_name":"commutability","score":0.6150000095367432},{"id":"https://openalex.org/keywords/analytical-performance","display_name":"analytical performance","score":0.3659999966621399}],"concepts":[{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6391128897666931},{"id":"https://openalex.org/C48921125","wikidata":"https://www.wikidata.org/wiki/Q10861030","display_name":"Linear regression","level":2,"score":0.6015284061431885},{"id":"https://openalex.org/C77170095","wikidata":"https://www.wikidata.org/wiki/Q1753188","display_name":"Linearity","level":2,"score":0.5413695573806763},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.51329106092453},{"id":"https://openalex.org/C202799725","wikidata":"https://www.wikidata.org/wiki/Q272035","display_name":"Accuracy and precision","level":2,"score":0.513279914855957},{"id":"https://openalex.org/C83546350","wikidata":"https://www.wikidata.org/wiki/Q1139051","display_name":"Regression","level":2,"score":0.4635433852672577},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4576406180858612},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.4111464321613312},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.39437589049339294},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.07331383228302002},{"id":"https://openalex.org/C119599485","wikidata":"https://www.wikidata.org/wiki/Q43035","display_name":"Electrical engineering","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":"Q000706","qualifier_name":"statistics & numerical data","is_major_topic":false},{"descriptor_ui":"D011453","descriptor_name":"Prostaglandins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011453","descriptor_name":"Prostaglandins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012044","descriptor_name":"Regression Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013929","descriptor_name":"Thromboxane B2","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D013929","descriptor_name":"Thromboxane B2","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013929","descriptor_name":"Thromboxane B2","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015121","descriptor_name":"6-Ketoprostaglandin F1 alpha","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015121","descriptor_name":"6-Ketoprostaglandin F1 alpha","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015230","descriptor_name":"Prostaglandin D2","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D015230","descriptor_name":"Prostaglandin D2","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015230","descriptor_name":"Prostaglandin D2","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015232","descriptor_name":"Dinoprostone","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015232","descriptor_name":"Dinoprostone","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015237","descriptor_name":"Dinoprost","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D015237","descriptor_name":"Dinoprost","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015237","descriptor_name":"Dinoprost","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.4155/bio.11.58","doi":"https://doi.org/10.4155/bio.11.58","is_oa":false,"landing_page_url":"https://doi.org/10.4155/bio.11.58","pdf_url":null,"source":{"id":"https://openalex.org/S159955403","display_name":"Bioanalysis","issn_l":"1757-6180","issn":["1757-6180","1757-6199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"journal-article"},{"id":"pmid:21510759","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21510759","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6607647579","display_name":null,"funder_award_id":"R25 GM62011","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G8639553344","display_name":"The TU-CCP Academic Community: Expanding Opportunities","funder_award_id":"5r25gm062011-07","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1968454575","https://openalex.org/W1973845698","https://openalex.org/W1976512498","https://openalex.org/W1980198114","https://openalex.org/W1991656581","https://openalex.org/W1993016161","https://openalex.org/W2001379166","https://openalex.org/W2015795623","https://openalex.org/W2081221505","https://openalex.org/W2121035292","https://openalex.org/W2121688201","https://openalex.org/W2126679046","https://openalex.org/W2144348065","https://openalex.org/W2144447216","https://openalex.org/W2162392926","https://openalex.org/W2743411124","https://openalex.org/W3098668365","https://openalex.org/W4246731685"],"related_works":["https://openalex.org/W1983741550","https://openalex.org/W2243340754","https://openalex.org/W7162786880","https://openalex.org/W7098740534","https://openalex.org/W3179001980","https://openalex.org/W2367310674","https://openalex.org/W2085063455","https://openalex.org/W313879549","https://openalex.org/W2127452446","https://openalex.org/W4386959850"],"abstract_inverted_index":{"BACKGROUND:":[0],"As":[1],"new":[2],"methods":[3,114,121,213,264,279],"are":[4,119,214,265,280],"developed":[5],"to":[6,16,22,37,53,83,104,259],"increase":[7],"efficiency":[8],"and":[9,45,76,90,99,112,115,134,153,156,179,187,202,236,242,250,271],"higher":[10,85],"analytical":[11,29,97,132],"performance,":[12],"it":[13,34],"is":[14,35,71,137,171,190],"necessary":[15],"evaluate":[17],"their":[18],"quality":[19],"in":[20,96,226],"comparison":[21,129,160],"standard":[23],"methods.":[24,181,257],"To":[25,123],"understand":[26],"how":[27],"the":[28,39,54,60,67,72,78,110,117,120,131,145,162,166,177,183,188,212,228,231,255,262,277],"performance":[30,133],"changes":[31],"between":[32,254],"methods,":[33,229],"common":[36],"compare":[38,49],"validation":[40],"parameters;":[41],"sensitivity,":[42],"linearity,":[43],"accuracy":[44,184],"precision.":[46],"Here,":[47],"we":[48],"an":[50],"UHPLC-UV":[51],"method":[52,56,105,135],"HPLC-UV":[55],"(reference":[57],"method)":[58],"for":[59,74,176,195,217,239,246,268],"simultaneous":[61],"determination":[62],"of":[63,130,161,169,233],"seven":[64],"prostanoids.":[65],"Though":[66],"basic":[68],"chromatography":[69],"theory":[70],"same":[73],"HPLC":[75,113,178],"UHPLC,":[77],"instrumentation":[79,98],"has":[80],"been":[81],"modified":[82],"accommodate":[84],"pressures,":[86],"lower":[87],"flow":[88],"rates":[89],"smaller":[91],"sample":[92],"size.":[93],"The":[94,220,273],"differences":[95],"procedures":[100],"can":[101],"give":[102],"rise":[103],"inequivalencies.":[106],"Our":[107],"approach":[108],"evaluates":[109],"UHPLC":[111,180],"poses":[116],"question:":[118],"equivalent?":[122],"answer":[124],"this":[125],"question":[126],"a":[127,234],"statistical":[128],"parameters":[136],"necessary.":[138],"RESULTS:":[139],"Statistical":[140,159],"comparisons":[141],"were":[142],"performed":[143],"using":[144],"t-test,":[146],"F-test,":[147],"regression":[148,152,209],"analyses":[149,275],"(ordinary":[150],"linear":[151,208],"Deming":[154,221,260],"regression)":[155],"Bland-Altman":[157,274],"analyses.":[158],"results,":[163],"suggested":[164],"that":[165,211],"precision":[167],"(amount":[168],"variability)":[170],"different":[172],"(p":[173,192],"<":[174],"0.05)":[175,194],"Whereas,":[182],"(method":[185],"bias":[186,238,245],"means)":[189],"similar":[191,267],">":[193],"8-isoprostane,":[196,247],"11-dehydro":[197,240],"TXB\u2082,":[198],"PGE\u2082":[199],"PGF(2\u03b1),":[200,248],"PGD\u2082":[201,249],"15-deoxy":[203,251],"\u0394\u00b9\u00b2,\u00b9\u2074":[204],"PGJ\u2082.":[205],"DISCUSSION:":[206],"Ordinary":[207],"shows":[210],"well":[215],"correlated":[216],"all":[218],"compounds.":[219],"regression,":[222,261],"which":[223],"assumes":[224],"error":[225],"both":[227],"suggests":[230],"existence":[232],"proportional":[235,244],"constant":[237],"TXB\u2082":[241],"only":[243],"\u0394(12,14)":[252],"PGJ\u2082":[253],"two":[256,263,278],"According":[258],"statistically":[266],"6-keto":[269],"PGF(1\u03b1)":[270],"PGE\u2082.":[272],"indicate":[276],"commutable.":[281]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}