{"id":"https://openalex.org/W2017271523","doi":"https://doi.org/10.1016/0014-5793(94)00940-6","title":"High level expression and characterisation of Plasmepsin II, an aspartic proteinase from Plasmodium falciparum","display_name":"High level expression and characterisation of Plasmepsin II, an aspartic proteinase from Plasmodium falciparum","publication_year":1994,"publication_date":"1994-09-26","ids":{"openalex":"https://openalex.org/W2017271523","doi":"https://doi.org/10.1016/0014-5793(94)00940-6","mag":"2017271523","pmid":"https://pubmed.ncbi.nlm.nih.gov/7925966"},"language":"en","primary_location":{"id":"doi:10.1016/0014-5793(94)00940-6","doi":"https://doi.org/10.1016/0014-5793(94)00940-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/0014-5793(94)00940-6","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2894%2900940-6","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2894%2900940-6","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102070896","display_name":"Jeffrey Hill","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I97429440","display_name":"University of Wales","ror":"https://ror.org/01se4f844","country_code":"GB","type":"education","lineage":["https://openalex.org/I97429440"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jeffrey Hill","raw_affiliation_strings":["Department of Biochemistry, University of Wales College of Cardiff, UK","Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, UK","institution_ids":["https://openalex.org/I97429440"]},{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK","institution_ids":["https://openalex.org/I97429440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086520363","display_name":"Lorraine Tyas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I97429440","display_name":"University of Wales","ror":"https://ror.org/01se4f844","country_code":"GB","type":"education","lineage":["https://openalex.org/I97429440"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Lorraine Tyas","raw_affiliation_strings":["Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK","institution_ids":["https://openalex.org/I97429440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060558381","display_name":"Lowri H. Phylip","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I97429440","display_name":"University of Wales","ror":"https://ror.org/01se4f844","country_code":"GB","type":"education","lineage":["https://openalex.org/I97429440"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Lowri H. Phylip","raw_affiliation_strings":["Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK","institution_ids":["https://openalex.org/I97429440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112058728","display_name":"John Kay","orcid":"https://orcid.org/0000-0002-2615-8506","observed_orcids":["https://orcid.org/0000-0002-2615-8506"]},"institutions":[{"id":"https://openalex.org/I97429440","display_name":"University of Wales","ror":"https://ror.org/01se4f844","country_code":"GB","type":"education","lineage":["https://openalex.org/I97429440"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"John Kay","raw_affiliation_strings":["Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK","institution_ids":["https://openalex.org/I97429440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009470180","display_name":"Ben M. Dunn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ben M. Dunn","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, J. Hillis Miller Health Center, University of Florida, Gainesville, FL 32610, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, J. Hillis Miller Health Center, University of Florida, Gainesville, FL 32610, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000664349","display_name":"Colin Berry","orcid":"https://orcid.org/0000-0002-9943-548X","observed_orcids":["https://orcid.org/0000-0002-9943-548X"]},"institutions":[{"id":"https://openalex.org/I97429440","display_name":"University of Wales","ror":"https://ror.org/01se4f844","country_code":"GB","type":"education","lineage":["https://openalex.org/I97429440"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Colin Berry","raw_affiliation_strings":["Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, PO Box 903, Cardiff CF1 1ST, Wales, UK","institution_ids":["https://openalex.org/I97429440"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":7.1924,"has_fulltext":true,"cited_by_count":126,"citation_normalized_percentile":{"value":0.96982404,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"352","issue":"2","first_page":"155","last_page":"158"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.9047999978065491,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.9047999978065491,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.06700000166893005,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.007600000128149986,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasmepsin-ii","display_name":"plasmepsin II","score":0.968999981880188},{"id":"https://openalex.org/keywords/plasmodium-falciparum","display_name":"Plasmodium falciparum","score":0.9810000061988831},{"id":"https://openalex.org/keywords/aspartic-protease","display_name":"aspartic protease","score":0.9860000014305115},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7770000100135803},{"id":"https://openalex.org/keywords/autoactivation","display_name":"self-activation","score":0.9049999713897705},{"id":"https://openalex.org/keywords/renin-like-activity","display_name":"renin-like activity","score":0.4390000104904175},{"id":"https://openalex.org/keywords/chromogenic-peptide-substrates","display_name":"chromogenic peptide substrates","score":0.8040000200271606},{"id":"https://openalex.org/keywords/ion-exchange-purification","display_name":"ion exchange purification","score":0.703000009059906}],"concepts":[{"id":"https://openalex.org/C2778371730","wikidata":"https://www.wikidata.org/wiki/Q311383","display_name":"Plasmodium falciparum","level":3,"score":0.730707585811615},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5498450398445129},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.49267202615737915},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.47127506136894226},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.43690919876098633},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3916008770465851},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37735894322395325},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C2778048844","wikidata":"https://www.wikidata.org/wiki/Q12156","display_name":"Malaria","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010963","descriptor_name":"Plasmodium falciparum","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010963","descriptor_name":"Plasmodium falciparum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016282","descriptor_name":"Aspartic Acid Endopeptidases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D016282","descriptor_name":"Aspartic Acid Endopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016282","descriptor_name":"Aspartic Acid Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017125","descriptor_name":"Genes, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017421","descriptor_name":"Sequence Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/0014-5793(94)00940-6","doi":"https://doi.org/10.1016/0014-5793(94)00940-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/0014-5793(94)00940-6","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2894%2900940-6","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:7925966","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7925966","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Journal Article"},{"id":"pmh:oai:https://orca.cardiff.ac.uk:64829","doi":null,"is_oa":false,"landing_page_url":"https://orca.cardiff.ac.uk/id/eprint/64829/","pdf_url":null,"source":{"id":"https://openalex.org/S4306401195","display_name":"ORCA Online Research @Cardiff (Cardiff University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79510175","host_organization_name":"Cardiff University","host_organization_lineage":["https://openalex.org/I79510175"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1016/0014-5793(94)00940-6","doi":"https://doi.org/10.1016/0014-5793(94)00940-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/0014-5793(94)00940-6","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/0014-5793%2894%2900940-6","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310009","display_name":"University of Florida","ror":"https://ror.org/02y3ad647"},{"id":"https://openalex.org/F4320320006","display_name":"Royal Society","ror":"https://ror.org/03wnrjx87"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2017271523.pdf","grobid_xml":"https://content.openalex.org/works/W2017271523.grobid-xml"},"referenced_works_count":20,"referenced_works":["https://openalex.org/W1522940126","https://openalex.org/W1574059019","https://openalex.org/W1575704306","https://openalex.org/W1678391582","https://openalex.org/W1866017395","https://openalex.org/W1873199501","https://openalex.org/W1984716929","https://openalex.org/W1998431888","https://openalex.org/W2011897552","https://openalex.org/W2024728695","https://openalex.org/W2031796366","https://openalex.org/W2057331661","https://openalex.org/W2090632234","https://openalex.org/W2095439551","https://openalex.org/W2100837269","https://openalex.org/W2152967629","https://openalex.org/W2153701967","https://openalex.org/W2281541306","https://openalex.org/W2493316313","https://openalex.org/W2764540609"],"related_works":["https://openalex.org/W2073963375","https://openalex.org/W1999818915","https://openalex.org/W2166986819","https://openalex.org/W2163385165","https://openalex.org/W2092945615","https://openalex.org/W2010594485","https://openalex.org/W1967752532","https://openalex.org/W1581608952","https://openalex.org/W7100158182","https://openalex.org/W2624573020"],"abstract_inverted_index":{"DNA":[0],"encoding":[1],"the":[2,7,10,20,49],"last":[3],"48":[4],"residues":[5],"of":[6,14,42,68,83],"propart":[8],"and":[9],"whole":[11],"mature":[12,63],"sequence":[13],"Plasmepsin":[15,64,89],"II":[16,90],"was":[17,34,51,59,77,91],"inserted":[18],"into":[19],"T7":[21],"dependent":[22],"vector":[23],"pET":[24],"3a":[25],"for":[26],"expression":[27],"in":[28],"E.":[29],"coli.":[30],"The":[31,66],"resultant":[32],"product":[33],"insoluble":[35],"but":[36],"accumulated":[37],"at":[38],"approximately":[39,84],"20":[40],"mg/l":[41],"cell":[43],"culture.":[44],"Following":[45],"solubilisation":[46],"with":[47],"urea,":[48],"zymogen":[50],"refolded":[52],"and,":[53],"after":[54],"purification":[55],"by":[56,95],"ion-exchange":[57],"chromatography,":[58],"autoactivated":[60],"to":[61,71,86],"generate":[62],"II.":[65],"ability":[67],"this":[69],"enzyme":[70],"hydrolyse":[72],"several":[73],"chromogenic":[74],"peptide":[75],"substrates":[76],"examined;":[78],"despite":[79],"an":[80],"overall":[81],"identity":[82],"35%":[85],"human":[87],"renin,":[88],"not":[92],"inhibited":[93],"significantly":[94],"renin":[96],"inhibitors.":[97]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":5},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
