{"id":"https://openalex.org/W2017222268","doi":"https://doi.org/10.2298/hel0440025m","title":"Agrobacterium-mediated transformation of two high oleic sunflower (helianthus annuus l) genotypes: Assessment and optimization of important parameters","display_name":"Agrobacterium-mediated transformation of two high oleic sunflower (helianthus annuus l) genotypes: Assessment and optimization of important parameters","publication_year":2004,"publication_date":"2004-01-01","ids":{"openalex":"https://openalex.org/W2017222268","doi":"https://doi.org/10.2298/hel0440025m","mag":"2017222268"},"language":"en","primary_location":{"id":"doi:10.2298/hel0440025m","doi":"https://doi.org/10.2298/hel0440025m","is_oa":true,"landing_page_url":"https://doi.org/10.2298/hel0440025m","pdf_url":"http://www.doiserbia.nb.rs/ft.aspx?id=1018-18060440025M","source":{"id":"https://openalex.org/S59292991","display_name":"Helia","issn_l":"1018-1806","issn":["1018-1806","2197-0483"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310321164","host_organization_name":"University of Novi Sad","host_organization_lineage":["https://openalex.org/P4310321164"],"host_organization_lineage_names":["University of Novi Sad"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Helia","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://www.doiserbia.nb.rs/ft.aspx?id=1018-18060440025M","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102166984","display_name":"SH Mohamed","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135140700","display_name":"University of Bonn","ror":"https://ror.org/041nas322","country_code":"DE","type":"education","lineage":["https://openalex.org/I135140700"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sh Mohamed","raw_affiliation_strings":["Institute of Plant Molecular Physiology and Biotechnology, University of Bonn, Karlrobert-Kreiten-Str. 13, 53115 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Molecular Physiology and Biotechnology, University of Bonn, Karlrobert-Kreiten-Str. 13, 53115 Bonn, Germany","institution_ids":["https://openalex.org/I135140700"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038601153","display_name":"Robert Boehm","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135140700","display_name":"University of Bonn","ror":"https://ror.org/041nas322","country_code":"DE","type":"education","lineage":["https://openalex.org/I135140700"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"R. Boehm","raw_affiliation_strings":["Institute of Plant Molecular Physiology and Biotechnology, University of Bonn, Karlrobert-Kreiten-Str. 13, 53115 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Molecular Physiology and Biotechnology, University of Bonn, Karlrobert-Kreiten-Str. 13, 53115 Bonn, Germany","institution_ids":["https://openalex.org/I135140700"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091806353","display_name":"Pedro Canisio Binsfeld","orcid":"https://orcid.org/0000-0002-1858-3879","observed_orcids":["https://orcid.org/0000-0002-1858-3879"]},"institutions":[{"id":"https://openalex.org/I135140700","display_name":"University of Bonn","ror":"https://ror.org/041nas322","country_code":"DE","type":"education","lineage":["https://openalex.org/I135140700"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"P.C. Binsfeld","raw_affiliation_strings":["Institute of Plant Molecular Physiology and Biotechnology , University of Bonn , Karlrobert-Kreiten-Str. 13 , 53115 Bonn , Germany","University of Bonn"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Molecular Physiology and Biotechnology , University of Bonn , Karlrobert-Kreiten-Str. 13 , 53115 Bonn , Germany","institution_ids":["https://openalex.org/I135140700"]},{"raw_affiliation_string":"University of Bonn","institution_ids":["https://openalex.org/I135140700"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113619450","display_name":"H. Schnabl","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135140700","display_name":"University of Bonn","ror":"https://ror.org/041nas322","country_code":"DE","type":"education","lineage":["https://openalex.org/I135140700"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"H. Schnabl","raw_affiliation_strings":["Institute of Plant Molecular Physiology and Biotechnology, University of Bonn, Karlrobert-Kreiten-Str. 13, 53115 Bonn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Molecular Physiology and Biotechnology, University of Bonn, Karlrobert-Kreiten-Str. 13, 53115 Bonn, Germany","institution_ids":["https://openalex.org/I135140700"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I135140700"],"apc_list":null,"apc_paid":null,"fwci":0.4121,"has_fulltext":true,"cited_by_count":12,"citation_normalized_percentile":{"value":0.68804693,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"27","issue":"40","first_page":"25","last_page":"40"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12742","display_name":"Sunflower and Safflower Cultivation","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/agrobacterium-mediated-transformation","display_name":"Agrobacterium-mediated transformation","score":0.9789999723434448},{"id":"https://openalex.org/keywords/high-oleic-sunflower","display_name":"high-oleic sunflower","score":0.9919999837875366},{"id":"https://openalex.org/keywords/sunflower","display_name":"sunflower","score":0.996999979019165},{"id":"https://openalex.org/keywords/plant-regeneration","display_name":"plant regeneration","score":0.8420000076293945},{"id":"https://openalex.org/keywords/acetosyringone","display_name":"acetosyringone","score":0.8420000076293945},{"id":"https://openalex.org/keywords/transformation-protocol-optimization","display_name":"transformation protocol optimization","score":0.46799999475479126}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8308707475662231},{"id":"https://openalex.org/C2780874738","wikidata":"https://www.wikidata.org/wiki/Q906309","display_name":"Acetosyringone","level":5,"score":0.7700644731521606},{"id":"https://openalex.org/C2778181364","wikidata":"https://www.wikidata.org/wiki/Q171497","display_name":"Helianthus annuus","level":3,"score":0.7667756080627441},{"id":"https://openalex.org/C2779197568","wikidata":"https://www.wikidata.org/wiki/Q423986","display_name":"Sunflower","level":2,"score":0.7500002384185791},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.6879228353500366},{"id":"https://openalex.org/C2776162087","wikidata":"https://www.wikidata.org/wiki/Q131472","display_name":"Agrobacterium tumefaciens","level":4,"score":0.656259298324585},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.6293395757675171},{"id":"https://openalex.org/C76725341","wikidata":"https://www.wikidata.org/wiki/Q1893126","display_name":"Inbred strain","level":3,"score":0.5591250061988831},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.5091208815574646},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.4727295935153961},{"id":"https://openalex.org/C180032290","wikidata":"https://www.wikidata.org/wiki/Q1664296","display_name":"Inoculation","level":2,"score":0.4479835629463196},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.40526437759399414},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.21152830123901367},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.17789652943611145},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.2298/hel0440025m","doi":"https://doi.org/10.2298/hel0440025m","is_oa":true,"landing_page_url":"https://doi.org/10.2298/hel0440025m","pdf_url":"http://www.doiserbia.nb.rs/ft.aspx?id=1018-18060440025M","source":{"id":"https://openalex.org/S59292991","display_name":"Helia","issn_l":"1018-1806","issn":["1018-1806","2197-0483"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310321164","host_organization_name":"University of Novi Sad","host_organization_lineage":["https://openalex.org/P4310321164"],"host_organization_lineage_names":["University of Novi Sad"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Helia","raw_type":"journal-article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.954.6291","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.954.6291","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.doiserbia.nb.rs/%28X%281%29A%28YytzYUDn0QEkAAAAOWEyNWM0ZTAtYjZjZS00Mzg0LTgzODYtNThiYWQxYTM1OTQ3Fe3Dcslz9Dqh7_tiAi2dbGLvDKc1%29%29/img/doi/1018-1806/2004/1018-18060440025M.pdf","raw_type":"text"}],"best_oa_location":{"id":"doi:10.2298/hel0440025m","doi":"https://doi.org/10.2298/hel0440025m","is_oa":true,"landing_page_url":"https://doi.org/10.2298/hel0440025m","pdf_url":"http://www.doiserbia.nb.rs/ft.aspx?id=1018-18060440025M","source":{"id":"https://openalex.org/S59292991","display_name":"Helia","issn_l":"1018-1806","issn":["1018-1806","2197-0483"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310321164","host_organization_name":"University of Novi Sad","host_organization_lineage":["https://openalex.org/P4310321164"],"host_organization_lineage_names":["University of Novi Sad"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Helia","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9208999872207642,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2017222268.pdf","grobid_xml":"https://content.openalex.org/works/W2017222268.grobid-xml"},"referenced_works_count":41,"referenced_works":["https://openalex.org/W24759977","https://openalex.org/W63689919","https://openalex.org/W110453976","https://openalex.org/W163297827","https://openalex.org/W332043012","https://openalex.org/W1513485285","https://openalex.org/W1539503448","https://openalex.org/W1574233575","https://openalex.org/W1900333706","https://openalex.org/W1964223840","https://openalex.org/W1967092449","https://openalex.org/W1968572229","https://openalex.org/W1969745850","https://openalex.org/W1975081504","https://openalex.org/W1977766787","https://openalex.org/W1986809770","https://openalex.org/W1994470176","https://openalex.org/W1997039789","https://openalex.org/W2024556021","https://openalex.org/W2026437110","https://openalex.org/W2029476089","https://openalex.org/W2034524970","https://openalex.org/W2039930593","https://openalex.org/W2041655605","https://openalex.org/W2045741785","https://openalex.org/W2050085223","https://openalex.org/W2056008136","https://openalex.org/W2066385431","https://openalex.org/W2091488708","https://openalex.org/W2094300768","https://openalex.org/W2112234428","https://openalex.org/W2118547343","https://openalex.org/W2123457047","https://openalex.org/W2128635872","https://openalex.org/W2136796097","https://openalex.org/W2164827714","https://openalex.org/W2168255317","https://openalex.org/W2234698338","https://openalex.org/W2505426135","https://openalex.org/W2995048110","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W7101135794","https://openalex.org/W2116626298","https://openalex.org/W7017157890","https://openalex.org/W2160046166","https://openalex.org/W2182096588","https://openalex.org/W7009852040","https://openalex.org/W2234698338","https://openalex.org/W2349325991","https://openalex.org/W2181440077","https://openalex.org/W2885665226"],"abstract_inverted_index":{"The":[0,28,43,99],"establishment":[1],"of":[2,35,96,169,196],"an":[3],"efficient":[4],"Agrobacterium\u2013mediated":[5],"transformation":[6,52,66,119],"protocol":[7,29],"for":[8,45,51,72,78,150,163],"a":[9,139],"recalcitrant":[10],"crop":[11],"like":[12],"high":[13],"oleic":[14],"sunflower":[15],"genotypes,":[16],"cv.":[17,73,106,178],"Capella":[18,74,107,179],"and":[19,33,40,75,108,113,133,175,180],"SWSR2":[20,109,181],"inbred":[21,80,110,182],"line,":[22,183],"is":[23],"presented":[24],"in":[25,105,177],"this":[26],"paper.":[27],"requires":[30],"the":[31,47,54,79,85,89,94,124,148,157,167,170,188,194],"identification":[32],"optimization":[34],"parameters":[36],"affecting":[37],"T-DNA":[38],"delivery":[39],"plant":[41,61],"regeneration.":[42],"basis":[44],"identifying":[46],"most":[48],"appropriate":[49],"conditions":[50],"was":[53,67,103],"fluorometric,":[55],"histochemical":[56],"GUS":[57,101,171],"activity":[58,102],"coupled":[59],"with":[60,88,143],"cell":[62],"vitality.":[63],"Agrobacterium":[64],"tumefaciens\u2013mediated":[65],"performed":[68],"using":[69,135],"strain":[70,76],"LBA4404":[71],"GV3101":[77],"line.":[81],"Both":[82],"strains":[83],"harboring":[84],"pBI121":[86],"plasmid":[87],"gus":[90],"gene":[91],"were":[92],"under":[93],"control":[95],"35S":[97],"promoter.":[98],"flourometric":[100],"increased":[104,166],"line":[111],"1.9":[112],"1.6":[114],"fold,":[115],"respectively,":[116],"relative":[117],"to":[118,127,173],"without":[120],"inducers":[121],"by":[122,129,134,161],"optimizing":[123],"bacterial":[125,158],"density":[126],"OD600=1.0,":[128],"splitting":[130],"shoot":[131],"apices":[132],"MS":[136],"medium":[137,141,155],"as":[138,191,193],"co-cultivation":[140,162],"supplemented":[142],"200":[144],"\u00b5M":[145],"acetosyringone.":[146],"Pre-culturing":[147],"explants":[149],"three":[151],"days":[152],"on":[153],"SIM2":[154],"before":[156],"inoculation":[159],"followed":[160],"72":[164],"h":[165],"percentage":[168,190],"expression":[172],"40%":[174],"30%":[176],"respectively.":[184],"It":[185],"also":[186],"improved":[187],"regeneration":[189],"well":[192],"vitality":[195],"cells.":[197]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}