{"id":"https://openalex.org/W2017193591","doi":"https://doi.org/10.1117/12.239506","title":"Micromanipulation and physiological monitoring of cells using two-photon excited fluorescence in cw laser tweezers","display_name":"Micromanipulation and physiological monitoring of cells using two-photon excited fluorescence in cw laser tweezers","publication_year":1996,"publication_date":"1996-05-10","ids":{"openalex":"https://openalex.org/W2017193591","doi":"https://doi.org/10.1117/12.239506","mag":"2017193591"},"language":"en","primary_location":{"id":"doi:10.1117/12.239506","doi":"https://doi.org/10.1117/12.239506","is_oa":true,"landing_page_url":"https://doi.org/10.1117/12.239506","pdf_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/2678/0000/Micromanipulation-and-physiological-monitoring-of-cells-using-two-photon-excited/10.1117/12.239506.pdf","source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/2678/0000/Micromanipulation-and-physiological-monitoring-of-cells-using-two-photon-excited/10.1117/12.239506.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112210229","display_name":"G. J. Sonek","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210133819","display_name":"Beckman Laser Institute and Medical Clinic","ror":"https://ror.org/03bfp2076","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210133819"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gregory J. Sonek","raw_affiliation_strings":["Beckman Laser Institute and Medical Clinic (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beckman Laser Institute and Medical Clinic (United States)","institution_ids":["https://openalex.org/I4210133819"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024013249","display_name":"Yagang Liu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210133819","display_name":"Beckman Laser Institute and Medical Clinic","ror":"https://ror.org/03bfp2076","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210133819"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yagang Liu","raw_affiliation_strings":["Beckman Laser Institute and Medical Clinic (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beckman Laser Institute and Medical Clinic (United States)","institution_ids":["https://openalex.org/I4210133819"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084427519","display_name":"Michael W. Berns","orcid":"https://orcid.org/0000-0002-9640-2885","observed_orcids":["https://orcid.org/0000-0002-9640-2885"]},"institutions":[{"id":"https://openalex.org/I4210133819","display_name":"Beckman Laser Institute and Medical Clinic","ror":"https://ror.org/03bfp2076","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210133819"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael W. Berns","raw_affiliation_strings":["Beckman Laser Institute and Medical Clinic (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beckman Laser Institute and Medical Clinic (United States)","institution_ids":["https://openalex.org/I4210133819"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015656700","display_name":"Bruce J. Tromberg","orcid":"https://orcid.org/0000-0002-7481-7975","observed_orcids":["https://orcid.org/0000-0002-7481-7975"]},"institutions":[{"id":"https://openalex.org/I4210133819","display_name":"Beckman Laser Institute and Medical Clinic","ror":"https://ror.org/03bfp2076","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210133819"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bruce J. Tromberg","raw_affiliation_strings":["Beckman Laser Institute and Medical Clinic and Univ. of California (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beckman Laser Institute and Medical Clinic and Univ. of California (United States)","institution_ids":["https://openalex.org/I4210133819"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210133819"],"apc_list":null,"apc_paid":null,"fwci":2.7258,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.88606658,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"2678","issue":null,"first_page":"62","last_page":"68"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10490","display_name":"Orbital Angular Momentum in Optics","score":0.7627000212669373,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10490","display_name":"Orbital Angular Momentum in Optics","score":0.7627000212669373,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.14650000631809235,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11788","display_name":"Nonlinear Optical Materials Studies","score":0.04089999943971634,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/two-photon-fluorescence","display_name":"two-photon fluorescence","score":0.8190000057220459},{"id":"https://openalex.org/keywords/optical-tweezers","display_name":"optical tweezers","score":0.875},{"id":"https://openalex.org/keywords/continuous-wave-laser","display_name":"continuous-wave laser","score":0.7210000157356262},{"id":"https://openalex.org/keywords/cell-micromanipulation","display_name":"cell micromanipulation","score":0.8230000138282776},{"id":"https://openalex.org/keywords/cell-confinement","display_name":"cell confinement","score":0.8299999833106995},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.5799999833106995},{"id":"https://openalex.org/keywords/propidium-iodide","display_name":"propidium iodide","score":0.902999997138977},{"id":"https://openalex.org/keywords/spermatozoa","display_name":"spermatozoa","score":0.609000027179718},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.9100000262260437},{"id":"https://openalex.org/keywords/photodamage","display_name":"photodamage","score":0.902999997138977}],"concepts":[{"id":"https://openalex.org/C20198109","wikidata":"https://www.wikidata.org/wiki/Q1066633","display_name":"Optical tweezers","level":2,"score":0.7357146739959717},{"id":"https://openalex.org/C2775934118","wikidata":"https://www.wikidata.org/wiki/Q424169","display_name":"Propidium iodide","level":4,"score":0.7093524932861328},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6852511167526245},{"id":"https://openalex.org/C2778617687","wikidata":"https://www.wikidata.org/wiki/Q2448456","display_name":"Fluorophore","level":3,"score":0.6288851499557495},{"id":"https://openalex.org/C520434653","wikidata":"https://www.wikidata.org/wiki/Q38867","display_name":"Laser","level":2,"score":0.5943466424942017},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.559777021408081},{"id":"https://openalex.org/C47785574","wikidata":"https://www.wikidata.org/wiki/Q373859","display_name":"Two-photon excitation microscopy","level":3,"score":0.5567243695259094},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.4918639659881592},{"id":"https://openalex.org/C181500209","wikidata":"https://www.wikidata.org/wiki/Q215328","display_name":"Excited state","level":2,"score":0.46984022855758667},{"id":"https://openalex.org/C49040817","wikidata":"https://www.wikidata.org/wiki/Q193091","display_name":"Optoelectronics","level":1,"score":0.37587782740592957},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.24050867557525635},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.18617510795593262},{"id":"https://openalex.org/C184779094","wikidata":"https://www.wikidata.org/wiki/Q26383","display_name":"Atomic physics","level":1,"score":0.1628292202949524},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.0},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1117/12.239506","doi":"https://doi.org/10.1117/12.239506","is_oa":true,"landing_page_url":"https://doi.org/10.1117/12.239506","pdf_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/2678/0000/Micromanipulation-and-physiological-monitoring-of-cells-using-two-photon-excited/10.1117/12.239506.pdf","source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"}],"best_oa_location":{"id":"doi:10.1117/12.239506","doi":"https://doi.org/10.1117/12.239506","is_oa":true,"landing_page_url":"https://doi.org/10.1117/12.239506","pdf_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/2678/0000/Micromanipulation-and-physiological-monitoring-of-cells-using-two-photon-excited/10.1117/12.239506.pdf","source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2017193591.pdf","grobid_xml":"https://content.openalex.org/works/W2017193591.grobid-xml"},"referenced_works_count":13,"referenced_works":["https://openalex.org/W633291335","https://openalex.org/W1483227212","https://openalex.org/W2001886673","https://openalex.org/W2012406182","https://openalex.org/W2022817606","https://openalex.org/W2039285094","https://openalex.org/W2061464631","https://openalex.org/W2074870535","https://openalex.org/W2108461844","https://openalex.org/W2113372755","https://openalex.org/W2267384929","https://openalex.org/W2483244562","https://openalex.org/W6721623032"],"related_works":["https://openalex.org/W2108069322","https://openalex.org/W7061079575","https://openalex.org/W2120858305","https://openalex.org/W2415725618","https://openalex.org/W2062173957","https://openalex.org/W1964987544","https://openalex.org/W398056924","https://openalex.org/W2003621809","https://openalex.org/W2099089023","https://openalex.org/W2604031066"],"abstract_inverted_index":{"We":[0],"report":[1],"the":[2,31,41,90,126,147,151],"observation":[3],"of":[4,34,43,83,95,132,219],"two-photon":[5,114,226],"fluorescence":[6,60,141,164],"excitation":[7],"and":[8,21,57,159,180,203],"cell":[9,38,65,122,152],"confinement,":[10],"simultaneously,":[11],"in":[12,125,168],"a":[13,46,62,73,80,99,109,113,120,130,136,157,188],"continuous-wave":[14],"(cw)":[15],"single-beam":[16],"gradient":[17],"force":[18],"optical":[19,127,206,217],"trap,":[20],"demonstrate":[22],"its":[23],"use":[24],"as":[25,79],"an":[26,35],"in-situ":[27,215],"probe":[28],"to":[29,54,145,211],"study":[30],"physiological":[32,85],"state":[33],"optically":[36],"confined":[37,175],"sample.":[39],"At":[40],"wavelength":[42,94],"1064":[44],"nm,":[45],"single":[47],"focused":[48],"gaussian":[49],"laser":[50,107,178],"beam":[51,105],"is":[52],"used":[53,210],"simultaneously":[55],"confine,":[56],"excite":[58],"visible":[59],"from,":[61],"human":[63],"sperm":[64,121],"that":[66,77,150,173],"has":[67,153],"been":[68],"tagged":[69,199],"with":[70,112,182,200],"propidium":[71,184],"iodide,":[72],"exogenous":[74],"fluorescent":[75,201],"dye":[76,91,190],"functions":[78],"viability":[81,221],"assay":[82],"cellular":[84,220],"state.":[86,161],"The":[87],"intensity":[88],"at":[89,135],"peak":[92],"emission":[93,142],"620":[96],"nm":[97],"exhibits":[98],"near-square-law":[100],"dependence":[101],"on":[102],"incident":[103],"trapping":[104,138],"photon":[106],"power,":[108,139],"behavior":[110],"consistent":[111],"absorption":[115],"process.":[116],"In":[117],"addition,":[118],"for":[119,129,196],"held":[123],"stationary":[124],"tweezers":[128,179,207],"period":[131],"several":[133],"minutes":[134],"constant":[137],"red":[140],"was":[143,165],"observed":[144,167],"increase":[146],"time,":[148],"indicating":[149],"gradually":[154],"transitioned":[155],"between":[156],"live":[158],"dead":[160],"Two-photon":[162],"excited":[163],"also":[166],"Chinese":[169],"hamster":[170],"ovary":[171],"cells":[172],"were":[174],"by":[176],"cw":[177],"stained":[181],"either":[183],"iodide":[185],"or":[186,222],"Snarf,":[187],"pH-sensitive":[189],"probe.":[191],"These":[192],"results":[193],"suggest":[194],"that,":[195],"samples":[197],"suitably":[198],"probes":[202,218],"vital":[204],"stains,":[205],"can":[208],"be":[209],"generate":[212],"their":[213],"own":[214],"diagnostic":[216],"induced":[223],"photodamage,":[224],"via":[225],"processes.":[227]},"counts_by_year":[],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2016-06-24T00:00:00"}
