{"id":"https://openalex.org/W2016917153","doi":"https://doi.org/10.1159/000115888","title":"Purification and Characterization of the Major Oak Pollen Allergen Que a 1 for Component-Resolved Diagnostics Using ImmunoCAP\u00ae","display_name":"Purification and Characterization of the Major Oak Pollen Allergen Que a 1 for Component-Resolved Diagnostics Using ImmunoCAP\u00ae","publication_year":2008,"publication_date":"2008-01-01","ids":{"openalex":"https://openalex.org/W2016917153","doi":"https://doi.org/10.1159/000115888","mag":"2016917153","pmid":"https://pubmed.ncbi.nlm.nih.gov/18268388"},"language":"en","primary_location":{"id":"doi:10.1159/000115888","doi":"https://doi.org/10.1159/000115888","is_oa":false,"landing_page_url":"https://doi.org/10.1159/000115888","pdf_url":null,"source":{"id":"https://openalex.org/S205866209","display_name":"International Archives of Allergy and Immunology","issn_l":"1018-2438","issn":["1018-2438","1423-0097"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Archives of Allergy and Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019877696","display_name":"Robert Mov\u00e9rare","orcid":"https://orcid.org/0000-0001-6611-5036","observed_orcids":["https://orcid.org/0000-0001-6611-5036"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Robert Mov\u00e9rare","raw_affiliation_strings":["Phadia AB, Uppsala University, Uppsala, Sweden. robert.moverare@phadia.com","Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB, Uppsala University, Uppsala, Sweden. robert.moverare@phadia.com","institution_ids":["https://openalex.org/I123387679"]},{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024635644","display_name":"Henrik Everberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Henrik Everberg","raw_affiliation_strings":["Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078300733","display_name":"Raimo Carlsson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Raimo Carlsson","raw_affiliation_strings":["Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049058481","display_name":"Anette Holtz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Anette Holtz","raw_affiliation_strings":["Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087614518","display_name":"R. Thunberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Roland Thunberg","raw_affiliation_strings":["Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103370798","display_name":"Peter Olsson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Peter Olsson","raw_affiliation_strings":["Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022245180","display_name":"Peter Brostedt","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Peter Brostedt","raw_affiliation_strings":["Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038468550","display_name":"Erik H\u00f6gbom","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Erik H\u00f6gbom","raw_affiliation_strings":["Phadia AB and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Phadia AB and","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.1092,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.77264448,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"146","issue":"3","first_page":"203","last_page":"211"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10877","display_name":"Allergic Rhinitis and Sensitization","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10877","display_name":"Allergic Rhinitis and Sensitization","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10634","display_name":"Food Allergy and Anaphylaxis Research","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10051","display_name":"Asthma and respiratory diseases","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/allergen-purification","display_name":"allergen purification","score":0.9079999923706055},{"id":"https://openalex.org/keywords/component-resolved-diagnostics","display_name":"component-resolved diagnosis","score":0.8519999980926514},{"id":"https://openalex.org/keywords/immunocap","display_name":"ImmunoCAP","score":0.8759999871253967},{"id":"https://openalex.org/keywords/bet-v-1","display_name":"Bet v 1","score":0.8840000033378601},{"id":"https://openalex.org/keywords/profilin","display_name":"profilin","score":0.9240000247955322},{"id":"https://openalex.org/keywords/allergen-isoforms","display_name":"allergen isoforms","score":0.9020000100135803},{"id":"https://openalex.org/keywords/ige-inhibition","display_name":"IgE suppression","score":0.5059999823570251},{"id":"https://openalex.org/keywords/allergen-characterization","display_name":"allergen characterization","score":0.5199999809265137},{"id":"https://openalex.org/keywords/allergic-rhinitis","display_name":"allergic rhinitis","score":0.4320000112056732}],"concepts":[{"id":"https://openalex.org/C2780510475","wikidata":"https://www.wikidata.org/wiki/Q186752","display_name":"Allergen","level":3,"score":0.7768698334693909},{"id":"https://openalex.org/C2780618852","wikidata":"https://www.wikidata.org/wiki/Q79932","display_name":"Pollen","level":2,"score":0.6706373691558838},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.6586887836456299},{"id":"https://openalex.org/C168167062","wikidata":"https://www.wikidata.org/wiki/Q1117970","display_name":"Component (thermodynamics)","level":2,"score":0.424032062292099},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.35639962553977966},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34675753116607666},{"id":"https://openalex.org/C207480886","wikidata":"https://www.wikidata.org/wiki/Q42982","display_name":"Allergy","level":2,"score":0.34225502610206604},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.18953821063041687},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.09280824661254883},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006255","descriptor_name":"Rhinitis, Allergic, Seasonal","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D006255","descriptor_name":"Rhinitis, Allergic, Seasonal","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D006255","descriptor_name":"Rhinitis, Allergic, Seasonal","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D006255","descriptor_name":"Rhinitis, Allergic, Seasonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006255","descriptor_name":"Rhinitis, Allergic, Seasonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006255","descriptor_name":"Rhinitis, Allergic, Seasonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007073","descriptor_name":"Immunoglobulin E","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D007073","descriptor_name":"Immunoglobulin E","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D007073","descriptor_name":"Immunoglobulin E","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010865","descriptor_name":"Pilot Projects","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010865","descriptor_name":"Pilot Projects","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010865","descriptor_name":"Pilot Projects","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015180","descriptor_name":"Electrophoresis, Gel, Two-Dimensional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015180","descriptor_name":"Electrophoresis, Gel, Two-Dimensional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015180","descriptor_name":"Electrophoresis, Gel, Two-Dimensional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029963","descriptor_name":"Quercus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D029963","descriptor_name":"Quercus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D029963","descriptor_name":"Quercus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D029963","descriptor_name":"Quercus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D029963","descriptor_name":"Quercus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D029963","descriptor_name":"Quercus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D052179","descriptor_name":"Antigens, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D052179","descriptor_name":"Antigens, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D052179","descriptor_name":"Antigens, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1159/000115888","doi":"https://doi.org/10.1159/000115888","is_oa":false,"landing_page_url":"https://doi.org/10.1159/000115888","pdf_url":null,"source":{"id":"https://openalex.org/S205866209","display_name":"International Archives of Allergy and Immunology","issn_l":"1018-2438","issn":["1018-2438","1423-0097"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Archives of Allergy and Immunology","raw_type":"journal-article"},{"id":"pmid:18268388","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18268388","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International archives of allergy and immunology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W17496569","https://openalex.org/W127081487","https://openalex.org/W1542791634","https://openalex.org/W1668142298","https://openalex.org/W1751860473","https://openalex.org/W1774240040","https://openalex.org/W1970405818","https://openalex.org/W1972847057","https://openalex.org/W1976454430","https://openalex.org/W1978216986","https://openalex.org/W2007390733","https://openalex.org/W2008921531","https://openalex.org/W2014636062","https://openalex.org/W2017411827","https://openalex.org/W2042381017","https://openalex.org/W2043304722","https://openalex.org/W2044853699","https://openalex.org/W2046759597","https://openalex.org/W2047150341","https://openalex.org/W2049465161","https://openalex.org/W2051276205","https://openalex.org/W2055043387","https://openalex.org/W2067137500","https://openalex.org/W2069754887","https://openalex.org/W2076096345","https://openalex.org/W2079269856","https://openalex.org/W2099844881","https://openalex.org/W2105855231","https://openalex.org/W2109593104","https://openalex.org/W2109632654","https://openalex.org/W2115798299","https://openalex.org/W2127111231","https://openalex.org/W2130886030","https://openalex.org/W2144362290","https://openalex.org/W2158868184"],"related_works":["https://openalex.org/W2042874314","https://openalex.org/W2594585717","https://openalex.org/W2023847064","https://openalex.org/W4211202038","https://openalex.org/W1840213242","https://openalex.org/W2156868689","https://openalex.org/W2376340911","https://openalex.org/W1980302701","https://openalex.org/W2032428509","https://openalex.org/W2044944420"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,103,120,132,179,195],"aim":[2],"of":[3,23,105,136,213,225],"this":[4],"study":[5],"was":[6,41,109,115,122],"to":[7,17,27,73,83,167,177,183,191,221],"purify":[8],"the":[9,24],"major":[10],"oak":[11,44,74,229],"pollen":[12,45,75,230],"allergen,":[13],"Que":[14,33,38,168,217],"a":[15,34,39,85,107,112,141,144,158,169,185,206,215,218,240],"1,":[16,86,174],"perform":[18],"biochemical":[19],"and":[20,26,50,62,87,92,127,171,175,209],"immunological":[21],"characterization":[22],"allergen":[25,224],"develop":[28],"an":[29,223],"experimental":[30],"native":[31],"(n)":[32],"1":[35,40,91,108,142,170,186,219,234,241],"ImmunoCAP(R).":[36],"METHODS:":[37],"purified":[42,208],"from":[43,69,139],"extract":[46],"using":[47],"affinity":[48],"chromatography":[49],"characterized":[51,211],"by":[52,78,124],"SDS-PAGE,":[53],"two-dimensional":[54],"(2D)":[55],"PAGE,":[56],"mass":[57],"spectrometry":[58],"(MS),":[59],"N-terminal":[60,134],"sequencing":[61],"specific":[63],"IgE":[64,81,100,155,180,201],"inhibition":[65,156],"on":[66,117],"ImmunoCAP.":[67],"Samples":[68],"16":[70],"subjects":[71,164],"sensitized":[72,166],"were":[76,96,165,187],"analyzed":[77],"ImmunoCAP":[79,242],"for":[80],"reactivity":[82],"nQue":[84,106,140,184,214,239],"recombinant":[88],"(r)Bet":[89],"v":[90,173,193,233],"2":[93],"(profilin).":[94],"They":[95],"also":[97],"studied":[98],"in":[99,228,235,246],"immunoblotting.":[101,202],"RESULTS:":[102],"purity":[104],">95%,":[110],"since":[111],"single":[113],"band":[114],"observed":[116],"silver-stained":[118],"SDS-PAGE.":[119],"identity":[121,147],"verified":[123,157],"MS":[125],"analysis,":[126],"2D-PAGE":[128],"revealed":[129],"several":[130],"isoforms.":[131],"obtained":[133,196],"sequence":[135,146],"50-amino-acid":[137],"residues":[138],"showed":[143],"58-74%":[145],"with":[148,200],"other":[149],"pathogenesis-related":[150],"class":[151],"10":[152],"allergens.":[153],"Specific":[154],"preserved":[159],"immunoreactivity":[160],"(70-92%":[161],"inhibition).":[162],"All":[163],"Bet":[172,232],"two":[176],"profilin.":[178],"antibody":[181],"levels":[182],"generally":[188],"lower":[189],"than":[190],"rBet":[192],"1.":[194,216],"results":[197],"correlated":[198],"well":[199],"CONCLUSIONS:":[203],"We":[204],"present":[205],"highly":[207],"extensively":[210],"preparation":[212],"seems":[220],"be":[222,244],"equal":[226],"importance":[227],"as":[231],"birch":[236],"pollen.":[237],"An":[238],"will":[243],"useful":[245],"component-resolved":[247],"diagnostics.":[248]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2025-10-10T00:00:00"}