{"id":"https://openalex.org/W2016707540","doi":"https://doi.org/10.1002/elps.1150170517","title":"Protease\u2010catalyzed incorporation of 18O into peptide fragments and its application for protein sequencing by electrospray and matrix\u2010assisted laser desorption/ionization mass spectrometry","display_name":"Protease\u2010catalyzed incorporation of 18O into peptide fragments and its application for protein sequencing by electrospray and matrix\u2010assisted laser desorption/ionization mass spectrometry","publication_year":1996,"publication_date":"1996-01-01","ids":{"openalex":"https://openalex.org/W2016707540","doi":"https://doi.org/10.1002/elps.1150170517","mag":"2016707540","pmid":"https://pubmed.ncbi.nlm.nih.gov/8783021"},"language":"en","primary_location":{"id":"doi:10.1002/elps.1150170517","doi":"https://doi.org/10.1002/elps.1150170517","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.1150170517","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2016707540"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083171620","display_name":"Martina Schn\u00f6lzer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17937529","display_name":"German Cancer Research Center","ror":"https://ror.org/04cdgtt98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I17937529"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Martina Schn\u00f6lzer","raw_affiliation_strings":["Department of Cell Biology, German Cancer Research Center, Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, German Cancer Research Center, Heidelberg, Germany","institution_ids":["https://openalex.org/I17937529"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061872102","display_name":"Paul T. Jedrzejewski","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17937529","display_name":"German Cancer Research Center","ror":"https://ror.org/04cdgtt98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I17937529"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Paul Jedrzejewski","raw_affiliation_strings":["Central Spectroscopy Department, German Cancer Research Center, Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Spectroscopy Department, German Cancer Research Center, Heidelberg, Germany","institution_ids":["https://openalex.org/I17937529"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111818691","display_name":"Wolf Dieter Lehmann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17937529","display_name":"German Cancer Research Center","ror":"https://ror.org/04cdgtt98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I17937529"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Wolf D. Lehmann","raw_affiliation_strings":["Central Spectroscopy Department, German Cancer Research Center, Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Spectroscopy Department, German Cancer Research Center, Heidelberg, Germany","institution_ids":["https://openalex.org/I17937529"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5111818691"],"corresponding_institution_ids":["https://openalex.org/I17937529"],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":3.9518,"has_fulltext":false,"cited_by_count":293,"citation_normalized_percentile":{"value":0.95020408,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":100},"biblio":{"volume":"17","issue":"5","first_page":"945","last_page":"953"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.7612000107765198,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.7612000107765198,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.23549999296665192,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/18o-labeling","display_name":"18O labeling","score":0.7649999856948853},{"id":"https://openalex.org/keywords/protein-sequencing","display_name":"protein sequencing","score":0.609000027179718},{"id":"https://openalex.org/keywords/trypsin","display_name":"trypsin","score":0.8040000200271606},{"id":"https://openalex.org/keywords/chymotrypsin","display_name":"chymotrypsin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/post-source-decay","display_name":"post-source decay","score":0.6539999842643738},{"id":"https://openalex.org/keywords/esi-ms-ms","display_name":"electrospray ionization tandem mass spectrometry","score":0.7059999704360962}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8446255326271057},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.7132342457771301},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.6877772212028503},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.678767740726471},{"id":"https://openalex.org/C40684141","wikidata":"https://www.wikidata.org/wiki/Q902061","display_name":"Electrospray ionization","level":3,"score":0.6787388324737549},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.6412387490272522},{"id":"https://openalex.org/C2778401398","wikidata":"https://www.wikidata.org/wiki/Q383836","display_name":"Chymotrypsin","level":4,"score":0.5655794739723206},{"id":"https://openalex.org/C202157981","wikidata":"https://www.wikidata.org/wiki/Q7251488","display_name":"Protein mass spectrometry","level":4,"score":0.5205931663513184},{"id":"https://openalex.org/C27420735","wikidata":"https://www.wikidata.org/wiki/Q5968721","display_name":"Peptide mass fingerprinting","level":4,"score":0.5172458291053772},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.43008098006248474},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.38362348079681396},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.26886382699012756},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21281254291534424},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.10632503032684326},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D004723","descriptor_name":"Endorphins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007633","descriptor_name":"Keratins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008666","descriptor_name":"Metalloendopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009496","descriptor_name":"Neurotensin","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010103","descriptor_name":"Oxygen Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011761","descriptor_name":"Pyrrolidonecarboxylic Acid","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/elps.1150170517","doi":"https://doi.org/10.1002/elps.1150170517","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.1150170517","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},{"id":"pmid:8783021","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8783021","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W35752657","https://openalex.org/W312568510","https://openalex.org/W1968904921","https://openalex.org/W1972927753","https://openalex.org/W1984482878","https://openalex.org/W2027134607","https://openalex.org/W2049599518","https://openalex.org/W2058135122","https://openalex.org/W2080713587","https://openalex.org/W2098456588","https://openalex.org/W2399979644","https://openalex.org/W2406114471"],"related_works":["https://openalex.org/W2470889850","https://openalex.org/W2138542071","https://openalex.org/W1967438882","https://openalex.org/W2053502015","https://openalex.org/W4234184340","https://openalex.org/W2075403192","https://openalex.org/W2410350746","https://openalex.org/W1585879442","https://openalex.org/W2323208838","https://openalex.org/W2069996910"],"abstract_inverted_index":{"Proteins":[0],"were":[1],"digested":[2],"in":[3,52,83,89,113,154,243,271],"normal":[4],"and":[5,32,44,77,104,145,193,273],"highly":[6],"18O-enriched":[7],"water":[8],"using":[9],"proteases":[10,76,127,142],"commonly":[11],"employed":[12],"for":[13,59,212,265],"protein":[14,207,232,249],"sequencing.":[15],"The":[16,40],"extent":[17],"of":[18,49,56,86,93,110,117,190,199,215,230,239,268,287,318],"18O":[19,163],"incorporation":[20],"into":[21],"the":[22,60,90,94,123,131,141,175,213,228,237,244,260,266,282,302,316,319],"resulting":[23,51,82,112,153],"peptide":[24,61,69,156,185,246],"fragments":[25,95,159,186,195],"was":[26,138],"characterized":[27],"by":[28,221,236,251,296],"electrospray":[29],"ionization":[30],"(ESI)":[31],"matrix-assisted":[33],"laser":[34],"desorption/ionization":[35],"(MALDI)":[36],"mass":[37,54,115,188,197,252,283],"spectrometry":[38],"(MS).":[39],"endoproteinases":[41],"trypsin,":[42,143],"Lys-C":[43,146],"Glu-C":[45],"incorporate":[46,106,160],"two":[47],"atoms":[48],"18O,":[50,111],"a":[53,114,231,288],"shift":[55,116],"+4":[57,191],"D":[58,192],"fragments.":[62,157],"This":[63,312],"indicates":[64],"that,":[65],"following":[66],"proteolytic":[67],"cleavage,":[68],"products":[70],"continue":[71],"to":[72,181,306],"interact":[73],"with":[74,96,174,187,196],"these":[75,126,205],"undergo":[78,170],"repeated":[79],"binding/hydrolysis":[80,172],"cycles,":[81],"complete":[84],"equilibration":[85],"both":[87],"oxygens":[88],"carboxy":[91],"terminus":[92],"oxygen":[97],"from":[98],"solvent":[99],"water.":[100],"In":[101,135,226,248],"contrast,":[102],"chymotrypsin":[103],"Asp-N":[105],"only":[107,161,200],"one":[108,162],"atom":[109],"+2":[118,201],"D,":[119,300],"indicating":[120,165],"that":[121,140,166],"after":[122],"cleavage":[124],"step":[125],"do":[128,168],"not":[129,169],"accept":[130],"peptides":[132],"as":[133],"substrates.":[134],"addition,":[136,227],"it":[137,178],"found":[139],"Glu-C,":[144],"exhibit":[147],"minor":[148],"or":[149,223,298],"nontypical":[150,194],"sequence":[151],"specificities,":[152],"unexpected":[155],"These":[158],"atom,":[164],"they":[167],"further":[171],"cycles":[173],"enzyme.":[176],"Thus,":[177],"is":[179],"possible":[180],"discriminate":[182],"between":[183],"enzyme-typical":[184],"shifts":[189,198],"D.":[202,225],"Based":[203],"on":[204],"observations,":[206],"digest":[208,254],"strategies":[209],"are":[210],"described":[211],"generation":[214],"1:1":[216],"ion":[217,241,257,269,279,292],"doublets":[218,258,293],"spaced":[219,294],"either":[220,295],"2":[222,297],"4":[224,299],"C-terminus":[229],"can":[233],"be":[234],"identified":[235,320],"absence":[238],"an":[240,307],"doublet":[242],"corresponding":[245],"fragment.":[247],"sequencing":[250],"spectrometry,":[253],"protocols":[255],"generating":[256],"provide":[259],"most":[261],"clear-cut":[262],"analytical":[263],"results":[264],"recognition":[267],"series":[270,290,309],"ESI-MS/MS":[272],"MALDI":[274],"post-source":[275],"decay":[276],"(PSD)":[277],"product":[278],"spectra.":[280],"Only":[281],"spectrometric":[284],"fragment":[285,303],"ions":[286,304],"C-terminal":[289],"show":[291],"whereas":[301],"belonging":[305],"N-terminal":[308],"remain":[310],"unshifted.":[311],"assignment":[313],"unequivocally":[314],"reveals":[315],"direction":[317],"sequence.":[321]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":6},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":17},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":11}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}
