{"id":"https://openalex.org/W2016609072","doi":"https://doi.org/10.1016/0008-6215(86)85011-x","title":"Milligram to gram scale purification and characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F","display_name":"Milligram to gram scale purification and characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F","publication_year":1986,"publication_date":"1986-03-01","ids":{"openalex":"https://openalex.org/W2016609072","doi":"https://doi.org/10.1016/0008-6215(86)85011-x","mag":"2016609072","pmid":"https://pubmed.ncbi.nlm.nih.gov/2938733"},"language":"en","primary_location":{"id":"doi:10.1016/0008-6215(86)85011-x","doi":"https://doi.org/10.1016/0008-6215(86)85011-x","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0008-6215(86)85011-x","pdf_url":null,"source":{"id":"https://openalex.org/S34126442","display_name":"Carbohydrate Research","issn_l":"0008-6215","issn":["0008-6215","1873-426X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Carbohydrate Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000482958","display_name":"Arthur W. Miller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Arthur W. Miller","raw_affiliation_strings":["Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011 U.S.A","Department of Biochemistry & Biophysics, Iowa State University, Ames, Iowa 50011, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011 U.S.A","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Department of Biochemistry & Biophysics, Iowa State University, Ames, Iowa 50011, U.S.A","institution_ids":["https://openalex.org/I173911158"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031473798","display_name":"Steven H. Eklund","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven H. Eklund","raw_affiliation_strings":["Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011 U.S.A","Department of Biochemistry & Biophysics, Iowa State University, Ames, Iowa 50011, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011 U.S.A","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Department of Biochemistry & Biophysics, Iowa State University, Ames, Iowa 50011, U.S.A","institution_ids":["https://openalex.org/I173911158"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049579696","display_name":"John Francis Robyt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John F. Robyt","raw_affiliation_strings":["Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011 U.S.A","Department of Biochemistry & Biophysics, Iowa State University, Ames, Iowa 50011, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011 U.S.A","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Department of Biochemistry & Biophysics, Iowa State University, Ames, Iowa 50011, U.S.A","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5049579696"],"corresponding_institution_ids":["https://openalex.org/I173911158"],"apc_list":{"value":3800,"currency":"USD","value_usd":3800},"apc_paid":null,"fwci":3.4329,"has_fulltext":false,"cited_by_count":74,"citation_normalized_percentile":{"value":0.93028486,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"147","issue":"1","first_page":"119","last_page":"133"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8446999788284302,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8446999788284302,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0722000002861023,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.0421999990940094,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dextransucrase","display_name":"dextransucrase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/leuconostoc-mesenteroides","display_name":"Leuconostoc mesenteroides","score":0.9800000190734863},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8330000042915344},{"id":"https://openalex.org/keywords/levansucrase","display_name":"levansucrase","score":0.8309999704360962},{"id":"https://openalex.org/keywords/dextran-synthesis","display_name":"dextran synthesis","score":0.9010000228881836},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5809999704360962},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.7870000004768372},{"id":"https://openalex.org/keywords/isoenzymes","display_name":"isozymes","score":0.4950000047683716},{"id":"https://openalex.org/keywords/storage-stability","display_name":"storage stability","score":0.5809999704360962},{"id":"https://openalex.org/keywords/deae-cellulose-chromatography","display_name":"DEAE-cellulose chromatography","score":0.7369999885559082},{"id":"https://openalex.org/keywords/affinity-chromatography","display_name":"affinity chromatography","score":0.8429999947547913}],"concepts":[{"id":"https://openalex.org/C2777423746","wikidata":"https://www.wikidata.org/wiki/Q3706357","display_name":"Dextransucrase","level":5,"score":0.9916332364082336},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9268174171447754},{"id":"https://openalex.org/C2776183398","wikidata":"https://www.wikidata.org/wiki/Q10859028","display_name":"Dextranase","level":3,"score":0.8887118697166443},{"id":"https://openalex.org/C2777957631","wikidata":"https://www.wikidata.org/wiki/Q146954","display_name":"Leuconostoc mesenteroides","level":4,"score":0.8831683397293091},{"id":"https://openalex.org/C2777339698","wikidata":"https://www.wikidata.org/wiki/Q423123","display_name":"Dextran","level":2,"score":0.8424785733222961},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7178536057472229},{"id":"https://openalex.org/C163994747","wikidata":"https://www.wikidata.org/wiki/Q2916358","display_name":"Sephadex","level":3,"score":0.6322010159492493},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5005879402160645},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.4976368248462677},{"id":"https://openalex.org/C2780349071","wikidata":"https://www.wikidata.org/wiki/Q30836","display_name":"Levansucrase","level":4,"score":0.46980032324790955},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.45726287364959717},{"id":"https://openalex.org/C198684833","wikidata":"https://www.wikidata.org/wiki/Q2823748","display_name":"Specific activity","level":3,"score":0.4540588855743408},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42583373188972473},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.09098485112190247},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C2777272437","wikidata":"https://www.wikidata.org/wiki/Q131238","display_name":"Bacillus subtilis","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002848","descriptor_name":"Chromatography, DEAE-Cellulose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005964","descriptor_name":"Glucosyltransferases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005964","descriptor_name":"Glucosyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007934","descriptor_name":"Leuconostoc","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0008-6215(86)85011-x","doi":"https://doi.org/10.1016/0008-6215(86)85011-x","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0008-6215(86)85011-x","pdf_url":null,"source":{"id":"https://openalex.org/S34126442","display_name":"Carbohydrate Research","issn_l":"0008-6215","issn":["0008-6215","1873-426X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Carbohydrate Research","raw_type":"journal-article"},{"id":"pmid:2938733","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2938733","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Carbohydrate research","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4699565110","display_name":"BIOSYNTHETIC STUDY OF DENTAL PLAQUE POLYSACCHARIDES","funder_award_id":"R01DE003578","funder_id":"https://openalex.org/F4320337365","funder_display_name":"National Institute of Dental and Craniofacial Research"}],"funders":[{"id":"https://openalex.org/F4320337365","display_name":"National Institute of Dental and Craniofacial Research","ror":"https://ror.org/004a2wv92"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W16953744","https://openalex.org/W142144482","https://openalex.org/W1497245978","https://openalex.org/W1530959668","https://openalex.org/W1554917334","https://openalex.org/W1643481193","https://openalex.org/W1673197025","https://openalex.org/W1775749144","https://openalex.org/W1968834637","https://openalex.org/W1973445650","https://openalex.org/W1979559008","https://openalex.org/W1983726298","https://openalex.org/W1992252477","https://openalex.org/W1995054117","https://openalex.org/W1998247911","https://openalex.org/W2006399828","https://openalex.org/W2013404491","https://openalex.org/W2020826236","https://openalex.org/W2026801875","https://openalex.org/W2033756350","https://openalex.org/W2044205864","https://openalex.org/W2059202229","https://openalex.org/W2070175148","https://openalex.org/W2070948586","https://openalex.org/W2074367332","https://openalex.org/W2088315065","https://openalex.org/W2100837269","https://openalex.org/W2108934889","https://openalex.org/W2109563405","https://openalex.org/W2122757055","https://openalex.org/W2136466718","https://openalex.org/W2143640539","https://openalex.org/W2159944790","https://openalex.org/W2162502417","https://openalex.org/W2324926223","https://openalex.org/W4211060686","https://openalex.org/W4253329384"],"related_works":["https://openalex.org/W2006399828","https://openalex.org/W2038971474","https://openalex.org/W4239497709","https://openalex.org/W2013404491","https://openalex.org/W2398229792","https://openalex.org/W2767003495","https://openalex.org/W1968103801","https://openalex.org/W2005650303","https://openalex.org/W1969622078","https://openalex.org/W2160330880"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}