{"id":"https://openalex.org/W2016408814","doi":"https://doi.org/10.1021/bi052328g","title":"Transmembrane Helices 3 and 4 Are Involved in Substrate Recognition by the Na+/Dicarboxylate Cotransporter, NaDC1","display_name":"Transmembrane Helices 3 and 4 Are Involved in Substrate Recognition by the Na+/Dicarboxylate Cotransporter, NaDC1","publication_year":2006,"publication_date":"2006-01-25","ids":{"openalex":"https://openalex.org/W2016408814","doi":"https://doi.org/10.1021/bi052328g","mag":"2016408814","pmid":"https://pubmed.ncbi.nlm.nih.gov/16475819"},"language":"en","primary_location":{"id":"doi:10.1021/bi052328g","doi":"https://doi.org/10.1021/bi052328g","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi052328g","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090411334","display_name":"Naomi Oshiro","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]},{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Naomi Oshiro","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-0645, and Department of Integrative Biosciences, Oregon Health & Science University, Portland, Oregon 97239-3097"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-0645, and Department of Integrative Biosciences, Oregon Health & Science University, Portland, Oregon 97239-3097","institution_ids":["https://openalex.org/I165690674","https://openalex.org/I55302922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103627032","display_name":"Steven C. King","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]},{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven C. King","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-0645, and Department of Integrative Biosciences, Oregon Health & Science University, Portland, Oregon 97239-3097"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-0645, and Department of Integrative Biosciences, Oregon Health & Science University, Portland, Oregon 97239-3097","institution_ids":["https://openalex.org/I165690674","https://openalex.org/I55302922"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086969739","display_name":"Ana M. Pajor","orcid":"https://orcid.org/0000-0001-6696-1136","observed_orcids":["https://orcid.org/0000-0001-6696-1136"]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]},{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ana M. Pajor","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-0645, and Department of Integrative Biosciences, Oregon Health & Science University, Portland, Oregon 97239-3097"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-0645, and Department of Integrative Biosciences, Oregon Health & Science University, Portland, Oregon 97239-3097","institution_ids":["https://openalex.org/I165690674","https://openalex.org/I55302922"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5086969739"],"corresponding_institution_ids":["https://openalex.org/I165690674","https://openalex.org/I55302922"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.0399,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.76315425,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"45","issue":"7","first_page":"2302","last_page":"2310"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.8041999936103821,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.8041999936103821,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.12700000405311584,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.0340999998152256,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/succinate-transport","display_name":"succinate transport","score":0.9300000071525574},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.8870000243186951},{"id":"https://openalex.org/keywords/chimeric-transporters","display_name":"chimeric transporters","score":0.9290000200271606},{"id":"https://openalex.org/keywords/catalytic-efficiency","display_name":"catalytic efficiency","score":0.6190000176429749},{"id":"https://openalex.org/keywords/dicarboxylate-transporter","display_name":"dicarboxylate transporter","score":0.6940000057220459}],"concepts":[{"id":"https://openalex.org/C188053792","wikidata":"https://www.wikidata.org/wiki/Q3640664","display_name":"Cotransporter","level":3,"score":0.7307760715484619},{"id":"https://openalex.org/C118892022","wikidata":"https://www.wikidata.org/wiki/Q7834587","display_name":"Transmembrane domain","level":3,"score":0.5400487184524536},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.53622967004776},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.522284746170044},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5166821479797363},{"id":"https://openalex.org/C2994592520","wikidata":"https://www.wikidata.org/wiki/Q5090496","display_name":"Substrate specificity","level":3,"score":0.4314599633216858},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.35639262199401855},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3443392515182495},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.33632296323776245},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.3319190740585327},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.2672182321548462},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.20791402459144592},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.12978968024253845},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.06391191482543945},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005977","descriptor_name":"Glutarates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014982","descriptor_name":"Xenopus laevis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018408","descriptor_name":"Patch-Clamp Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D027981","descriptor_name":"Symporters","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D027981","descriptor_name":"Symporters","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D027981","descriptor_name":"Symporters","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D029363","descriptor_name":"Organic Anion Transporters, Sodium-Dependent","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D029363","descriptor_name":"Organic Anion Transporters, Sodium-Dependent","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D029363","descriptor_name":"Organic Anion Transporters, Sodium-Dependent","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D029382","descriptor_name":"Dicarboxylic Acid Transporters","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D029382","descriptor_name":"Dicarboxylic Acid Transporters","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D029382","descriptor_name":"Dicarboxylic Acid Transporters","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D050506","descriptor_name":"Mutant Chimeric Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi052328g","doi":"https://doi.org/10.1021/bi052328g","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi052328g","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:16475819","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16475819","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1786845918","display_name":null,"funder_award_id":"DK46269","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G4372629253","display_name":null,"funder_award_id":"NS 38226","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G4835014924","display_name":"AMPHIPATHIC STRUCTURES IN GABA TRANSPORT","funder_award_id":"R01NS038226","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G7750439751","display_name":"Molecular Mechanism of Renal Na+ Dependent Cotransport","funder_award_id":"R01DK046269","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1537111269","https://openalex.org/W1553933303","https://openalex.org/W1966871436","https://openalex.org/W1999052525","https://openalex.org/W2001473733","https://openalex.org/W2018200353","https://openalex.org/W2033687515","https://openalex.org/W2054999309","https://openalex.org/W2072772532","https://openalex.org/W2081943673","https://openalex.org/W2083905587","https://openalex.org/W2169608531","https://openalex.org/W2416766434","https://openalex.org/W2441654890"],"related_works":["https://openalex.org/W2003971246","https://openalex.org/W1970641010","https://openalex.org/W2000816692","https://openalex.org/W3015517115","https://openalex.org/W2011946987","https://openalex.org/W1965206006","https://openalex.org/W2018200353","https://openalex.org/W2325711973","https://openalex.org/W2054999309","https://openalex.org/W2739352345"],"abstract_inverted_index":{"The":[0,98,130,160],"Na(+)/dicarboxylate":[1],"cotransporters":[2],"(NaDC1)":[3],"from":[4,109],"mouse":[5,67],"(m)":[6],"and":[7,29,68,92,94,141,175,182,195],"rabbit":[8,69],"(rb)":[9],"differ":[10],"in":[11,31,42,56,74,80,122,168,198],"their":[12],"ability":[13],"to":[14,143],"handle":[15],"glutarate.":[16],"Substrate-dependent":[17],"inward":[18],"currents,":[19],"measured":[20],"using":[21,135],"two-electrode":[22],"voltage":[23],"clamp,":[24],"were":[25,44,132],"similar":[26],"for":[27,165],"glutarate":[28,41,57,81],"succinate":[30],"Xenopus":[32],"oocytes":[33],"expressing":[34],"mNaDC1.":[35],"In":[36],"contrast,":[37],"currents":[38],"evoked":[39],"by":[40,89],"rbNaDC1":[43,103,124],"only":[45],"about":[46],"5%":[47],"of":[48,63,102,106,115,152,192],"the":[49,83,95,145,157,169],"succinate-dependent":[50],"currents.":[51],"To":[52],"identify":[53],"domains":[54],"involved":[55],"transport,":[58,82],"we":[59],"constructed":[60],"a":[61,112,150],"series":[62],"chimeric":[64],"transporters":[65],"between":[66,172],"NaDC1.":[70,199],"Although":[71],"residues":[72,187],"found":[73],"multiple":[75],"transmembrane":[76],"helices":[77],"(TM)":[78],"participate":[79],"most":[84],"important":[85,190],"contribution":[86],"is":[87],"made":[88],"TM":[90,107,173,180],"3":[91,174,181],"4":[93,116,183],"associated":[96],"loops.":[97],"R(M3-4)":[99],"chimera,":[100],"consisting":[101],"with":[104,119,139,156],"substitution":[105],"3-4":[108],"mNaDC1,":[110],"had":[111],"decreased":[113],"K(0.5)(glutarate)":[114],"mM":[117,121],"compared":[118],"15":[120],"wild-type":[123],"without":[125],"any":[126],"effect":[127],"on":[128],"K(0.5)(succinate).":[129],"chimeras":[131],"also":[133],"characterized":[134],"dual-label":[136],"competitive":[137],"uptakes":[138],"(14)C-glutarate":[140],"(3)H-succinate":[142],"calculate":[144],"transport":[146],"specificity":[147,194],"ratio":[148],"(TSR),":[149],"measure":[151],"relative":[153],"catalytic":[154,196],"efficiency":[155,197],"two":[158],"substrates.":[159],"TSR":[161],"analysis":[162],"provides":[163],"evidence":[164],"functional":[166],"coupling":[167],"transition":[170],"state":[171],"4.":[176],"We":[177],"conclude":[178],"that":[179,188],"contain":[184],"amino":[185],"acid":[186],"are":[189],"determinants":[191],"substrate":[193]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}