{"id":"https://openalex.org/W2016372956","doi":"https://doi.org/10.1021/bi00090a018","title":"Role of electrostatic interactions in the binding of fluorescein by anti-fluorescein antibody 4-4-20","display_name":"Role of electrostatic interactions in the binding of fluorescein by anti-fluorescein antibody 4-4-20","publication_year":1993,"publication_date":"1993-10-05","ids":{"openalex":"https://openalex.org/W2016372956","doi":"https://doi.org/10.1021/bi00090a018","mag":"2016372956","pmid":"https://pubmed.ncbi.nlm.nih.gov/8399186"},"language":"en","primary_location":{"id":"doi:10.1021/bi00090a018","doi":"https://doi.org/10.1021/bi00090a018","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00090a018","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5037065001","display_name":"Vladimir G. Omelyanenko","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223532165","display_name":"University of Utah","ror":"https://ror.org/03r0ha626","country_code":"US","type":"education","lineage":["https://openalex.org/I223532165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vladimir G. Omelyanenko","raw_affiliation_strings":["Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City 84112"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City 84112","institution_ids":["https://openalex.org/I223532165"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031708632","display_name":"Wim Jiskoot","orcid":"https://orcid.org/0000-0002-1012-3456","observed_orcids":["https://orcid.org/0000-0002-1012-3456","https://orcid.org/0000-0001-7081-7890"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wim Jiskoot","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110906177","display_name":"James N. Herron","orcid":"https://orcid.org/0000-0002-8298-0537","observed_orcids":["https://orcid.org/0000-0002-8298-0537","https://orcid.org/0009-0002-0312-7798"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"James N. Herron","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":5.1294,"has_fulltext":false,"cited_by_count":39,"citation_normalized_percentile":{"value":0.95268831,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"32","issue":"39","first_page":"10423","last_page":"10429"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7904999852180481,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7904999852180481,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.11410000175237656,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.04230000078678131,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorescein","display_name":"fluorescein","score":0.9229999780654907},{"id":"https://openalex.org/keywords/antibody-antigen-interaction","display_name":"antigen-antibody reaction","score":0.8769999742507935},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.9980000257492065},{"id":"https://openalex.org/keywords/salt-bridges","display_name":"salt bridges","score":0.5809999704360962},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.6830000281333923},{"id":"https://openalex.org/keywords/binding-affinity","display_name":"binding affinity","score":0.6899999976158142},{"id":"https://openalex.org/keywords/fluorescence-lifetime","display_name":"fluorescence lifetime","score":0.7519999742507935},{"id":"https://openalex.org/keywords/hydrogen-bonding","display_name":"hydrogen bonding","score":0.6620000004768372},{"id":"https://openalex.org/keywords/interaction-free-energy","display_name":"free energy of interaction","score":0.7509999871253967},{"id":"https://openalex.org/keywords/molecular-recognition","display_name":"molecular recognition","score":0.6489999890327454}],"concepts":[{"id":"https://openalex.org/C2778447006","wikidata":"https://www.wikidata.org/wiki/Q1156474","display_name":"Icon","level":2,"score":0.8641862869262695},{"id":"https://openalex.org/C2778015030","wikidata":"https://www.wikidata.org/wiki/Q410922","display_name":"Fluorescein","level":3,"score":0.7853899002075195},{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.6525647044181824},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.5214171409606934},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4950091242790222},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.307853639125824},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.25506994128227234},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.24345514178276062},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.16700053215026855},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001666","descriptor_name":"Binding Sites, Antibody","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004563","descriptor_name":"Electrochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005452","descriptor_name":"Fluoresceins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005452","descriptor_name":"Fluoresceins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005453","descriptor_name":"Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019793","descriptor_name":"Fluorescein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00090a018","doi":"https://doi.org/10.1021/bi00090a018","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00090a018","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:8399186","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8399186","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.41999998688697815,"id":"https://metadata.un.org/sdg/7","display_name":"Affordable and clean energy"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8868013234","display_name":null,"funder_award_id":"AI22898","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W959106951","https://openalex.org/W1482005070","https://openalex.org/W1506094287","https://openalex.org/W1594409612","https://openalex.org/W1595699583","https://openalex.org/W1972618236","https://openalex.org/W1980711203","https://openalex.org/W1993714885","https://openalex.org/W1997886562","https://openalex.org/W1998783286","https://openalex.org/W2007054796","https://openalex.org/W2021163287","https://openalex.org/W2039545730","https://openalex.org/W2051843005","https://openalex.org/W2053649896","https://openalex.org/W2056417866","https://openalex.org/W2074534136","https://openalex.org/W2091544792","https://openalex.org/W2095453725","https://openalex.org/W2136741142"],"related_works":["https://openalex.org/W2040861628","https://openalex.org/W2056417866","https://openalex.org/W1977486703","https://openalex.org/W2091544792","https://openalex.org/W2018007357","https://openalex.org/W2269567618","https://openalex.org/W2040996863","https://openalex.org/W1595699583","https://openalex.org/W1997886562","https://openalex.org/W1594409612"],"abstract_inverted_index":{"Anti-fluorescein":[0],"antibodies":[1,32],"are":[2],"excellent":[3],"model":[4],"systems":[5],"for":[6,26,36,141],"studying":[7],"the":[8,46,61,102,108,114,119,130,146,158,180,202],"biochemical":[9],"basis":[10],"of":[11,18,49,52,64,68,94,104,116,121,145,150,182,201],"molecular":[12],"recognition":[13],"because":[14],"a":[15,50,69,92,187],"prodigious":[16],"amount":[17],"both":[19,208],"physico-chemical":[20],"and":[21,40,89,123,135,156,194,211,229],"structural":[22],"information":[23],"is":[24],"available":[25],"these":[27],"antibodies.":[28],"Furthermore,":[29,152],"recombinant":[30],"single-chain":[31],"have":[33,44],"been":[34],"produced":[35],"several":[37],"anti-fluorescein":[38,71],"antibodies,":[39],"site-specific":[41],"mutagenesis":[42],"studies":[43,79,127],"defined":[45],"energetic":[47],"contributions":[48],"number":[51],"key":[53],"active-site":[54],"residues.":[55],"In":[56,97],"previous":[57],"studies,":[58],"we":[59,100],"determined":[60,206],"three-dimensional":[62],"structure":[63],"an":[65,86],"antigen-binding":[66,124],"fragment":[67],"high-affinity":[70],"antibody":[72],"(4-4-20)":[73],"in":[74,85,91,107,137],"complex":[75,204],"with":[76],"fluorescein.":[77],"These":[78,126],"showed":[80,128],"that":[81,129],"fluorescein":[82,122,134],"binds":[83],"tightly":[84],"aromatic":[87],"slot":[88],"participates":[90],"network":[93],"electrostatic":[95,105],"interactions.":[96],"this":[98,177],"report,":[99],"examine":[101],"role":[103],"interactions":[106],"4-4-20":[109,138],"antigen-combining":[110],"site":[111],"by":[112,161],"observing":[113],"effects":[115],"pH":[117,154,209,212,223,235],"on":[118],"fluorescence":[120,199],"affinity.":[125],"salt":[131],"link":[132],"between":[133,190],"Arg-L34":[136],"probably":[139,185],"accounts":[140],"about":[142],"-1.5":[143],"kcal/mol-1":[144],"observed":[147,221,233],"free":[148],"energy":[149],"interaction.":[151],"at":[153,207,222,234],"10":[155],"higher,":[157],"affinity":[159],"decreases":[160],"more":[162],"than":[163],"100-fold":[164],"(delta":[165],"delta":[166],"G":[167],"degrees":[168],"approximately":[169],"equal":[170],"to":[171,179],"3":[172],"kcal":[173],"mol-1).":[174],"We":[175],"attributed":[176],"decrease":[178],"ionization":[181],"Tyr-L32,":[183],"which":[184],"disrupts":[186],"hydrogen":[188],"bond":[189],"tyrosine's":[191],"hydroxyl":[192],"group":[193],"fluorescein's":[195],"phenylcarboxylate":[196],"group.":[197],"The":[198],"lifetime":[200,216],"4-4-20/fluorescein":[203],"was":[205,220],"8":[210],"10.6.":[213],"Only":[214],"one":[215],"component":[217],"(0.38":[218],"ns)":[219,231],"8,":[224],"while":[225],"two":[226],"components":[227],"(0.3":[228],"3.4":[230],"were":[232],"10.6.(ABSTRACT":[236],"TRUNCATED":[237],"AT":[238],"250":[239],"WORDS)":[240]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}