{"id":"https://openalex.org/W2016238424","doi":"https://doi.org/10.1016/0165-2427(90)90031-m","title":"Cryopreservation of equine mononuclear cells for immunological studies","display_name":"Cryopreservation of equine mononuclear cells for immunological studies","publication_year":1990,"publication_date":"1990-06-01","ids":{"openalex":"https://openalex.org/W2016238424","doi":"https://doi.org/10.1016/0165-2427(90)90031-m","mag":"2016238424","pmid":"https://pubmed.ncbi.nlm.nih.gov/2378055"},"language":"en","primary_location":{"id":"doi:10.1016/0165-2427(90)90031-m","doi":"https://doi.org/10.1016/0165-2427(90)90031-m","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0165-2427(90)90031-m","pdf_url":null,"source":{"id":"https://openalex.org/S194416120","display_name":"Veterinary Immunology and Immunopathology","issn_l":"0165-2427","issn":["0165-2427","1873-2534"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-2","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Veterinary Immunology and Immunopathology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057856116","display_name":"Robert E. Truax","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R.E. Truax","raw_affiliation_strings":["School of Veterinary Medicine, Louisiana State University, Baton Rouge","Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Veterinary Medicine, Louisiana State University, Baton Rouge","institution_ids":["https://openalex.org/I121820613"]},{"raw_affiliation_string":"Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042877606","display_name":"Mary D. Powell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M.D. Powell","raw_affiliation_strings":["Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001716722","display_name":"Ronald C. Montelaro","orcid":"https://orcid.org/0000-0003-0147-3419","observed_orcids":["https://orcid.org/0000-0003-0147-3419"]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R.C. Montelaro","raw_affiliation_strings":["Department of Biochemistry, College of Basic Sciences, Louisiana State University, Baton Rouge, LA 70803 U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, College of Basic Sciences, Louisiana State University, Baton Rouge, LA 70803 U.S.A","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109360026","display_name":"Charles J. Issel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]},{"id":"https://openalex.org/I134386540","display_name":"Louisiana State University Agricultural Center","ror":"https://ror.org/01b8rza40","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613","https://openalex.org/I134386540"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C.J. Issel","raw_affiliation_strings":["Department of Veterinary Science, Louisiana Agricultural Experiment Station, Baton Rouge, LA 70803 U.S.A","Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Science, Louisiana Agricultural Experiment Station, Baton Rouge, LA 70803 U.S.A","institution_ids":["https://openalex.org/I134386540"]},{"raw_affiliation_string":"Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088019995","display_name":"Mark J. Newman","orcid":"https://orcid.org/0000-0002-0433-6690","observed_orcids":["https://orcid.org/0000-0002-0433-6690"]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"M.J. Newman","raw_affiliation_strings":["Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA 70803 U.S.A","institution_ids":["https://openalex.org/I121820613"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5088019995"],"corresponding_institution_ids":["https://openalex.org/I121820613"],"apc_list":{"value":3250,"currency":"USD","value_usd":3250},"apc_paid":null,"fwci":0.1643,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.44379609,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"25","issue":"2","first_page":"139","last_page":"153"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.3537999987602234,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.3537999987602234,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10872","display_name":"Veterinary Equine Medical Research","score":0.15719999372959137,"subfield":{"id":"https://openalex.org/subfields/3402","display_name":"Equine"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12322","display_name":"Animal health and immunology","score":0.08139999955892563,"subfield":{"id":"https://openalex.org/subfields/3404","display_name":"Small Animals"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cryopreservation","display_name":"cryopreservation","score":0.9150000214576721},{"id":"https://openalex.org/keywords/buffy-coat","display_name":"buffy coat","score":0.7839999794960022},{"id":"https://openalex.org/keywords/dmso-cryoprotectant","display_name":"DMSO cryoprotectant","score":0.8450000286102295},{"id":"https://openalex.org/keywords/cell-recovery","display_name":"cell recovery","score":0.5789999961853027},{"id":"https://openalex.org/keywords/mitogen-induced-proliferation","display_name":"mitogen-induced proliferation","score":0.7059999704360962},{"id":"https://openalex.org/keywords/pokeweed-mitogen","display_name":"pokeweed mitogen","score":0.7850000262260437},{"id":"https://openalex.org/keywords/phytohemagglutinin","display_name":"phytohemagglutinin","score":0.9789999723434448},{"id":"https://openalex.org/keywords/concanavalin-a","display_name":"concanavalin A","score":1.0},{"id":"https://openalex.org/keywords/lymphocyte-subsets","display_name":"lymphocyte subsets","score":0.5730000138282776},{"id":"https://openalex.org/keywords/complement-dependent-cytotoxicity","display_name":"complement-dependent cytotoxicity","score":0.7699999809265137},{"id":"https://openalex.org/keywords/cell-surface-antigen-expression","display_name":"cell surface antigen expression","score":0.6000000238418579}],"concepts":[{"id":"https://openalex.org/C137061746","wikidata":"https://www.wikidata.org/wiki/Q736033","display_name":"Peripheral blood mononuclear cell","level":3,"score":0.8097715377807617},{"id":"https://openalex.org/C179933525","wikidata":"https://www.wikidata.org/wiki/Q1144664","display_name":"Cryopreservation","level":3,"score":0.8068908452987671},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7068338990211487},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.570683479309082},{"id":"https://openalex.org/C2778285947","wikidata":"https://www.wikidata.org/wiki/Q548079","display_name":"Buffy coat","level":2,"score":0.5206224918365479},{"id":"https://openalex.org/C19869320","wikidata":"https://www.wikidata.org/wiki/Q419277","display_name":"Concanavalin A","level":3,"score":0.5137029886245728},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4567069709300995},{"id":"https://openalex.org/C5362183","wikidata":"https://www.wikidata.org/wiki/Q410200","display_name":"Ficoll","level":4,"score":0.45485591888427734},{"id":"https://openalex.org/C2781005381","wikidata":"https://www.wikidata.org/wiki/Q7208633","display_name":"Pokeweed mitogen","level":4,"score":0.44249534606933594},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4308242201805115},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.4206163287162781},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.4145849645137787},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.3849961459636688},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3603619933128357},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.19694367051124573},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.16683733463287354},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.10232430696487427},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08223846554756165},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.06883570551872253}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000954","descriptor_name":"Antigens, Surface","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003602","descriptor_name":"Cytotoxicity, Immunologic","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006650","descriptor_name":"Histocompatibility Testing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006736","descriptor_name":"Horses","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007963","descriptor_name":"Leukocytes, Mononuclear","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D007963","descriptor_name":"Leukocytes, Mononuclear","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D008934","descriptor_name":"Mitogens","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015395","descriptor_name":"Histocompatibility Antigens Class I","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D015925","descriptor_name":"Cryopreservation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/0165-2427(90)90031-m","doi":"https://doi.org/10.1016/0165-2427(90)90031-m","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0165-2427(90)90031-m","pdf_url":null,"source":{"id":"https://openalex.org/S194416120","display_name":"Veterinary Immunology and Immunopathology","issn_l":"0165-2427","issn":["0165-2427","1873-2534"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-2","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Veterinary Immunology and Immunopathology","raw_type":"journal-article"},{"id":"pmid:2378055","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2378055","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Veterinary immunology and immunopathology","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8801988673","display_name":null,"funder_award_id":"A125850","funder_id":"https://openalex.org/F4320332505","funder_display_name":"U.S. Public Health Service"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W16193925","https://openalex.org/W116912075","https://openalex.org/W314762497","https://openalex.org/W1501728375","https://openalex.org/W1967827134","https://openalex.org/W1969024863","https://openalex.org/W1969903488","https://openalex.org/W1986257637","https://openalex.org/W1987077239","https://openalex.org/W1989959561","https://openalex.org/W2003033924","https://openalex.org/W2041301477","https://openalex.org/W2054673126","https://openalex.org/W2060842246","https://openalex.org/W2066203649","https://openalex.org/W2067609410","https://openalex.org/W2087529389","https://openalex.org/W2157929710","https://openalex.org/W2273253613","https://openalex.org/W2403912611","https://openalex.org/W2416672317","https://openalex.org/W2470581012","https://openalex.org/W2764469125","https://openalex.org/W4407210797","https://openalex.org/W4409121486"],"related_works":["https://openalex.org/W2442858511","https://openalex.org/W1902093698","https://openalex.org/W4409121486","https://openalex.org/W2031391416","https://openalex.org/W2905446998","https://openalex.org/W2063834204","https://openalex.org/W4210898517","https://openalex.org/W7142637550","https://openalex.org/W2550756069","https://openalex.org/W2060610238"],"abstract_inverted_index":null,"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}