{"id":"https://openalex.org/W2016196699","doi":"https://doi.org/10.1034/j.1600-0722.2003.00043.x","title":"Dentin sialoprotein isoforms: detection and characterization of a high molecular weight dentin sialoprotein","display_name":"Dentin sialoprotein isoforms: detection and characterization of a high molecular weight dentin sialoprotein","publication_year":2003,"publication_date":"2003-05-22","ids":{"openalex":"https://openalex.org/W2016196699","doi":"https://doi.org/10.1034/j.1600-0722.2003.00043.x","mag":"2016196699","pmid":"https://pubmed.ncbi.nlm.nih.gov/12786955"},"language":"en","primary_location":{"id":"doi:10.1034/j.1600-0722.2003.00043.x","doi":"https://doi.org/10.1034/j.1600-0722.2003.00043.x","is_oa":false,"landing_page_url":"https://doi.org/10.1034/j.1600-0722.2003.00043.x","pdf_url":null,"source":{"id":"https://openalex.org/S40136177","display_name":"European Journal Of Oral Sciences","issn_l":"0029-845X","issn":["0029-845X","0909-8836","1600-0722"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Oral Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084800178","display_name":"Chunlin Qin","orcid":"https://orcid.org/0000-0002-2906-9074","observed_orcids":["https://orcid.org/0000-0002-2906-9074"]},"institutions":[{"id":"https://openalex.org/I919571938","display_name":"The University of Texas Health Science Center at Houston","ror":"https://ror.org/03gds6c39","country_code":"US","type":"education","lineage":["https://openalex.org/I919571938"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Chunlin Qin","raw_affiliation_strings":["Department of Basic Sciences, The University of Texas-Houston Health Science Center, Dental Branch, 6516 M.D. Anderson Boulevard, DBB, Room 4.133, Houston, TX 77030, USA. Chunlin.Qin@uth.tmc.edu","Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Basic Sciences, The University of Texas-Houston Health Science Center, Dental Branch, 6516 M.D. Anderson Boulevard, DBB, Room 4.133, Houston, TX 77030, USA. Chunlin.Qin@uth.tmc.edu","institution_ids":["https://openalex.org/I919571938"]},{"raw_affiliation_string":"Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;","institution_ids":["https://openalex.org/I919571938"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037045318","display_name":"Jan C. Brunn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I919571938","display_name":"The University of Texas Health Science Center at Houston","ror":"https://ror.org/03gds6c39","country_code":"US","type":"education","lineage":["https://openalex.org/I919571938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jan C. Brunn","raw_affiliation_strings":["Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;","institution_ids":["https://openalex.org/I919571938"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111516396","display_name":"Otto Baba","orcid":"https://orcid.org/0009-0005-6444-2945","observed_orcids":["https://orcid.org/0009-0005-6444-2945"]},"institutions":[{"id":"https://openalex.org/I919571938","display_name":"The University of Texas Health Science Center at Houston","ror":"https://ror.org/03gds6c39","country_code":"US","type":"education","lineage":["https://openalex.org/I919571938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Otto Baba","raw_affiliation_strings":["Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;","institution_ids":["https://openalex.org/I919571938"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006637900","display_name":"James N. Wygant","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James N. Wygant","raw_affiliation_strings":["Department of Immunology, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Immunology, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042878383","display_name":"Bradley W. McIntyre","orcid":"https://orcid.org/0000-0002-1042-4064","observed_orcids":["https://orcid.org/0000-0002-1042-4064"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bradley W. McIntyre","raw_affiliation_strings":["Department of Immunology, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Immunology, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113712383","display_name":"William T. Butler","orcid":"https://orcid.org/0000-0003-3366-587X","observed_orcids":["https://orcid.org/0000-0003-3366-587X"]},"institutions":[{"id":"https://openalex.org/I919571938","display_name":"The University of Texas Health Science Center at Houston","ror":"https://ror.org/03gds6c39","country_code":"US","type":"education","lineage":["https://openalex.org/I919571938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William T. Butler","raw_affiliation_strings":["Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Basic Sciences, The University of Texas\u2013Houston Health Science Center, Dental Branch, Houston, Texas, USA;","institution_ids":["https://openalex.org/I919571938"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5084800178"],"corresponding_institution_ids":["https://openalex.org/I919571938"],"apc_list":{"value":4220,"currency":"USD","value_usd":4220},"apc_paid":null,"fwci":2.325,"has_fulltext":false,"cited_by_count":62,"citation_normalized_percentile":{"value":0.86980937,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"111","issue":"3","first_page":"235","last_page":"242"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12110","display_name":"Bone and Dental Protein Studies","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12110","display_name":"Bone and Dental Protein Studies","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10113","display_name":"Dental materials and restorations","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/3505","display_name":"Orthodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dentin-sialoprotein","display_name":"dentin sialoprotein","score":1.0},{"id":"https://openalex.org/keywords/protein-isoforms","display_name":"protein isoforms","score":0.8550000190734863},{"id":"https://openalex.org/keywords/n-glycosylation","display_name":"N-glycosylation","score":0.824999988079071},{"id":"https://openalex.org/keywords/phosphate-content","display_name":"phosphate content","score":0.6779999732971191},{"id":"https://openalex.org/keywords/glycoprotein-characterization","display_name":"glycoprotein characterization","score":0.5960000157356262}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7149897813796997},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5922608375549316},{"id":"https://openalex.org/C2779263046","wikidata":"https://www.wikidata.org/wiki/Q189637","display_name":"Dentin","level":2,"score":0.5278865098953247},{"id":"https://openalex.org/C2776432469","wikidata":"https://www.wikidata.org/wiki/Q14907671","display_name":"Bone sialoprotein","level":5,"score":0.5153100490570068},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.5074815154075623},{"id":"https://openalex.org/C87933860","wikidata":"https://www.wikidata.org/wiki/Q898506","display_name":"Edman degradation","level":4,"score":0.47535082697868347},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.43996188044548035},{"id":"https://openalex.org/C96294017","wikidata":"https://www.wikidata.org/wiki/Q308708","display_name":"Isoelectric point","level":3,"score":0.42643845081329346},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4113295376300812},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.34912997484207153},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.22253507375717163},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.18454638123512268},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.08794233202934265},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07910731434822083},{"id":"https://openalex.org/C160160445","wikidata":"https://www.wikidata.org/wiki/Q24769688","display_name":"Alkaline phosphatase","level":3,"score":0.06898388266563416},{"id":"https://openalex.org/C131075544","wikidata":"https://www.wikidata.org/wiki/Q415863","display_name":"Osteocalcin","level":4,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000099311","descriptor_name":"Dentin Sialophosphoprotein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002241","descriptor_name":"Carbohydrates","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D003804","descriptor_name":"Dentin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010710","descriptor_name":"Phosphates","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010750","descriptor_name":"Phosphoproteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012795","descriptor_name":"Sialoglycoproteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D012795","descriptor_name":"Sialoglycoproteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016326","descriptor_name":"Extracellular Matrix Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D020033","descriptor_name":"Protein Isoforms","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1034/j.1600-0722.2003.00043.x","doi":"https://doi.org/10.1034/j.1600-0722.2003.00043.x","is_oa":false,"landing_page_url":"https://doi.org/10.1034/j.1600-0722.2003.00043.x","pdf_url":null,"source":{"id":"https://openalex.org/S40136177","display_name":"European Journal Of Oral Sciences","issn_l":"0029-845X","issn":["0029-845X","0909-8836","1600-0722"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Oral Sciences","raw_type":"journal-article"},{"id":"pmid:12786955","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12786955","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of oral sciences","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1154045490","display_name":null,"funder_award_id":"DE-05092","funder_id":"https://openalex.org/F4320337365","funder_display_name":"National Institute of Dental and Craniofacial Research"},{"id":"https://openalex.org/G247789771","display_name":"ADHESION MOLECULES IN HUMAN LEUKEMIA AND LYMPHOMA","funder_award_id":"5r01ca062596-07","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G799044023","display_name":null,"funder_award_id":"CA62596","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337365","display_name":"National Institute of Dental and Craniofacial Research","ror":"https://ror.org/004a2wv92"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W59499224","https://openalex.org/W1519536427","https://openalex.org/W1533283205","https://openalex.org/W1598651148","https://openalex.org/W1975540319","https://openalex.org/W1975732608","https://openalex.org/W1984555522","https://openalex.org/W1984812505","https://openalex.org/W2002701893","https://openalex.org/W2004402883","https://openalex.org/W2027743257","https://openalex.org/W2033841311","https://openalex.org/W2039705722","https://openalex.org/W2047841570","https://openalex.org/W2063189400","https://openalex.org/W2064603777","https://openalex.org/W2069987063","https://openalex.org/W2071395289","https://openalex.org/W2075328153","https://openalex.org/W2081733194","https://openalex.org/W2086026048","https://openalex.org/W2100837269","https://openalex.org/W2106021402","https://openalex.org/W2107564406","https://openalex.org/W2107892686","https://openalex.org/W2123779499","https://openalex.org/W2148760066","https://openalex.org/W2169546081","https://openalex.org/W2221760646","https://openalex.org/W4211066406"],"related_works":["https://openalex.org/W2064603777","https://openalex.org/W2135887801","https://openalex.org/W1966019688","https://openalex.org/W2075889889","https://openalex.org/W2021397270","https://openalex.org/W2048991232","https://openalex.org/W2002701893","https://openalex.org/W2092800605","https://openalex.org/W2039705722","https://openalex.org/W2017403819"],"abstract_inverted_index":{"Dentin":[0],"sialoprotein":[1],"(DSP)":[2],"is":[3],"a":[4,118],"glycoprotein":[5],"accounting":[6],"for":[7],"5-8%":[8],"of":[9,46,71,84,135,150,162,171],"the":[10,42,47,55,69,76,100,141,158],"dentin":[11,85],"non-collagenous":[12,86],"proteins.":[13],"The":[14],"cDNA":[15],"sequence":[16],"predicts":[17],"that":[18,59,68,103,140,157],"rat":[19,60],"DSP":[20,61,151],"has":[21,62],"13":[22],"potential":[23,30],"casein":[24],"kinase":[25],"phosphorylation":[26,37],"sites":[27],"and":[28,44,67,132],"six":[29],"N-linked":[31,79],"glycosylation":[32],"sites.":[33],"However,":[34],"its":[35,168],"total":[36],"level,":[38],"as":[39,41],"well":[40],"nature":[43],"locations":[45],"carbohydrate":[48,172],"moieties,":[49],"are":[50,73,148],"unknown.":[51],"Our":[52],"findings":[53],"in":[54,99],"present":[56],"study":[57],"show":[58],"6.2":[63],"phosphates":[64],"per":[65],"molecule":[66],"majority":[70],"carbohydrates":[72],"attached":[74],"to":[75],"protein":[77],"through":[78],"glycosylations.":[80],"During":[81],"our":[82],"separation":[83],"proteins":[87],"with":[88],"ion-exchange":[89],"chromatography,":[90],"we":[91],"observed":[92],"high":[93,113,144],"molecular":[94,114,145],"weight":[95,115,146],"components":[96,116,147],"eluting":[97],"late":[98],"salt":[101],"gradient":[102],"were":[104],"recognized":[105],"by":[106],"anti-DSP":[107,120],"antibodies.":[108],"We":[109],"have":[110],"purified":[111],"these":[112],"using":[117],"monoclonal":[119],"antibody":[121],"affinity":[122],"column.":[123],"Data":[124],"from":[125,167],"amino":[126],"acid":[127],"analysis,":[128],"phosphate":[129],"level":[130,170],"measurements":[131],"Edman":[133],"degradation":[134],"tryptic":[136],"peptides":[137],"unequivocally":[138],"proved":[139],"very":[142],"acidic,":[143],"isoforms":[149],"(designated":[152],"HMW-DSP).":[153],"Deglycosylation":[154],"analysis":[155],"indicates":[156],"slower":[159],"migration":[160],"rate":[161],"HMW-DSP":[163],"on":[164],"SDS-PAGE":[165],"results":[166],"higher":[169],"modifications.":[173]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":6}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}