{"id":"https://openalex.org/W2015996622","doi":"https://doi.org/10.1074/jbc.m606558200","title":"New Insights from the Structure-Function Analysis of the Catalytic Region of Human Platelet Phosphodiesterase 3A","display_name":"New Insights from the Structure-Function Analysis of the Catalytic Region of Human Platelet Phosphodiesterase 3A","publication_year":2006,"publication_date":"2006-07-28","ids":{"openalex":"https://openalex.org/W2015996622","doi":"https://doi.org/10.1074/jbc.m606558200","mag":"2015996622","pmid":"https://pubmed.ncbi.nlm.nih.gov/16873361"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m606558200","doi":"https://doi.org/10.1074/jbc.m606558200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m606558200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m606558200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003289248","display_name":"Su\u2010Hwi Hung","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Su-Hwi Hung","raw_affiliation_strings":["Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100441827","display_name":"Wei Zhang","orcid":"https://orcid.org/0009-0007-5347-417X","observed_orcids":["https://orcid.org/0009-0007-5347-417X","https://orcid.org/0000-0002-4315-8380"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wei Zhang","raw_affiliation_strings":["Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109057691","display_name":"Robin A. Pixley","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robin A. Pixley","raw_affiliation_strings":["Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111616185","display_name":"Bradford A. Jameson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I72816309","display_name":"Drexel University","ror":"https://ror.org/04bdffz58","country_code":"US","type":"education","lineage":["https://openalex.org/I72816309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bradford A. Jameson","raw_affiliation_strings":["Department of Biochemistry, Drexel University College of Medicine, Philadelphia, Pennsylvania 19104"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Drexel University College of Medicine, Philadelphia, Pennsylvania 19104","institution_ids":["https://openalex.org/I72816309"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Yu Chu Huang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yu Chu Huang","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716","Department of Chemistry & Biochemistry, University of Delaware, Newark, Delaware 19716"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Department of Chemistry & Biochemistry, University of Delaware, Newark, Delaware 19716","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111830511","display_name":"Roberta F. Colman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]},{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Roberta F. Colman","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716","Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140","Department of Chemistry & Biochemistry, University of Delaware, Newark, Delaware 19716"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"Department of Chemistry & Biochemistry, University of Delaware, Newark, Delaware 19716","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"last","author":{"id":null,"display_name":"Robert W. Colman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]},{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Robert W. Colman","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716","Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I84392919","https://openalex.org/I86501945"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.1358,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.47878357,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"281","issue":"39","first_page":"29236","last_page":"29244"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phosphodiesterase-3a","display_name":"PDE3A","score":0.8090000152587891},{"id":"https://openalex.org/keywords/pde3b","display_name":"PDE3B","score":0.8180000185966492},{"id":"https://openalex.org/keywords/substrate-binding-site","display_name":"substrate binding site","score":0.7839999794960022},{"id":"https://openalex.org/keywords/camp-hydrolysis","display_name":"cAMP hydrolysis","score":0.7590000033378601},{"id":"https://openalex.org/keywords/platelet-function","display_name":"platelet function","score":0.7360000014305115},{"id":"https://openalex.org/keywords/catalytic-mechanism","display_name":"catalytic mechanism","score":0.5600000023841858},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.3140000104904175}],"concepts":[{"id":"https://openalex.org/C62826618","wikidata":"https://www.wikidata.org/wiki/Q424304","display_name":"Phosphodiesterase","level":3,"score":0.7655612230300903},{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.6056350469589233},{"id":"https://openalex.org/C2984217123","wikidata":"https://www.wikidata.org/wiki/Q25099949","display_name":"Structure function","level":2,"score":0.5688790082931519},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.5405932664871216},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.5150480270385742},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4200386703014374},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3746981918811798},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36848869919776917},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27821624279022217},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.24375948309898376},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.23652055859565735},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22932597994804382},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.18128952383995056},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.14714491367340088},{"id":"https://openalex.org/C109214941","wikidata":"https://www.wikidata.org/wiki/Q18334","display_name":"Particle physics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015105","descriptor_name":"3',5'-Cyclic-AMP Phosphodiesterases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015105","descriptor_name":"3',5'-Cyclic-AMP Phosphodiesterases","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D015105","descriptor_name":"3',5'-Cyclic-AMP Phosphodiesterases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054684","descriptor_name":"Cyclic Nucleotide Phosphodiesterases, Type 3","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m606558200","doi":"https://doi.org/10.1074/jbc.m606558200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m606558200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:16873361","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16873361","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m606558200","doi":"https://doi.org/10.1074/jbc.m606558200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m606558200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2268750680","display_name":null,"funder_award_id":"HL-64943","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G7661021339","display_name":"MOLECULAR BASIS FOR PLATELET FUNCTION IN HEMOSTASIS","funder_award_id":"P01HL064943","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W99370438","https://openalex.org/W1531823431","https://openalex.org/W1912762104","https://openalex.org/W1963861627","https://openalex.org/W1965330483","https://openalex.org/W1967279802","https://openalex.org/W1983874809","https://openalex.org/W2013808156","https://openalex.org/W2021602387","https://openalex.org/W2029702791","https://openalex.org/W2036682103","https://openalex.org/W2043141059","https://openalex.org/W2050672068","https://openalex.org/W2056645340","https://openalex.org/W2060784660","https://openalex.org/W2062333049","https://openalex.org/W2063936526","https://openalex.org/W2070488805","https://openalex.org/W2071217711","https://openalex.org/W2082134056","https://openalex.org/W2085411745","https://openalex.org/W2088495869","https://openalex.org/W2130350603","https://openalex.org/W2132124951","https://openalex.org/W2154558722","https://openalex.org/W2161043271","https://openalex.org/W2164231548","https://openalex.org/W2170327826","https://openalex.org/W2172258989","https://openalex.org/W2248072176","https://openalex.org/W2408745332","https://openalex.org/W2414075848"],"related_works":["https://openalex.org/W2556933791","https://openalex.org/W2060784660","https://openalex.org/W2408745332","https://openalex.org/W2071217711","https://openalex.org/W2029702791","https://openalex.org/W2414075848","https://openalex.org/W1983874809","https://openalex.org/W2046764598","https://openalex.org/W1974113859","https://openalex.org/W2057405371"],"abstract_inverted_index":{"Human":[0],"phosphodiesterase":[1],"3A":[2],"(PDE3A)":[3],"degrades":[4],"cAMP,":[5],"the":[6,17,30,46,62,74,107,158,168,181,197,201,241,256,267,277],"major":[7],"inhibitor":[8],"of":[9,54,64,131,210],"platelet":[10,14],"function,":[11],"thus":[12],"potentiating":[13],"function.":[15],"Of":[16],"11":[18],"human":[19],"PDEs,":[20],"only":[21],"PDE3A":[22,43,65],"and":[23,85,92,112,152,171,176,220,262],"3B":[24],"have":[25],"44-amino":[26],"acid":[27,161],"inserts":[28],"in":[29,128,154,162,243,266,276],"catalytic":[31],"domain.":[32],"Their":[33],"function":[34],"is":[35,103,137,148,164,232],"not":[36,95,135,227],"clear.":[37],"Incubating":[38],"Sp-adenosine-3',5'-cyclic-S-(4-bromo-2,3-di-oxobutyl)":[39],"monophosphorothioate":[40],"(Sp-cAMPS-BDB)":[41],"with":[42,79,189,271],"irreversibly":[44,186],"inactivates":[45,90,187],"enzyme.":[47],"High":[48],"pressure":[49],"liquid":[50],"chromatography":[51],"(HPLC)":[52],"analysis":[53],"a":[55,69,205,236,249],"tryptic":[56],"digest":[57],"yielded":[58],"an":[59,122,140,223],"octapeptide":[60],"within":[61,73],"insert":[63,213,242,268],"((K)T(806)YNVTDDK(813)),":[66],"suggesting":[67,195],"that":[68,101,146,173,196,216,230],"substrate-binding":[70],"site":[71,279],"exists":[72],"insert.":[75],"Because":[76,157],"Sp-cAMPS-BDB":[77,89,151,185],"reacts":[78],"nucleophilic":[80],"residues,":[81],"mutants":[82,214],"Y807A,":[83],"D811A,":[84],"D812A":[86,93],"were":[87,178],"produced.":[88],"D811A":[91],"but":[94,125,134,226],"Y807A.":[96],"A":[97],"docking":[98],"model":[99],"showed":[100],"Tyr(807)":[102,147,261],"3.3":[104],"angstroms":[105,116],"from":[106,118],"reactive":[108],"carbon,":[109],"whereas":[110],"Asp(811)":[111],"Asp(812)":[113],"are":[114],">15":[115],"away":[117],"Sp-cAMPS-BDB.":[119],"Y807A":[120,136],"has":[121],"altered":[123,224],"K(m)":[124,175],"no":[126],"change":[127,253],"k(cat).":[129],"Activity":[130],"wild":[132,182,193],"type":[133],"inhibited":[138],"by":[139,150],"anti-insert":[141],"antibody.":[142],"These":[143],"data":[144],"suggest":[145],"modified":[149],"involved":[153],"substrate":[155,245,257],"binding.":[156],"homologous":[159],"amino":[160,264],"PDE3B":[163],"Cys(792),":[165],"we":[166],"prepared":[167],"mutant":[169],"Y807C":[170,188],"found":[172],"its":[174],"k(cat)":[177,225],"similar":[179,190],"to":[180,192,258,260,269],"type.":[183],"Moreover,":[184],"kinetics":[191],"type,":[194],"tyrosine":[198],"may,":[199],"like":[200],"cysteine,":[202],"serve":[203],"as":[204],"H":[206],"donor.":[207],"Kinetic":[208],"analyses":[209],"nine":[211],"additional":[212],"reveal":[215],"H782A,":[217],"T810A,":[218],"Y814A,":[219],"C816S":[221],"exhibit":[222],"K(m),":[228],"indicating":[229],"catalysis":[231,275],"modulated.":[233],"We":[234],"document":[235],"new":[237],"functional":[238],"role":[239],"for":[240,274],"which":[244],"binding":[246],"may":[247],"produce":[248],"conformational":[250],"change.":[251],"This":[252],"would":[254],"allow":[255],"bind":[259],"other":[263],"acids":[265],"interact":[270],"residues":[272],"important":[273],"active":[278],"cleft.":[280]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}