{"id":"https://openalex.org/W2015706285","doi":"https://doi.org/10.1139/o87-050","title":"The use of a monoclonal antibody for the rapid purification of kidney neutral endopeptidase (\"enkephalinase\") solubilized in octyl glucoside","display_name":"The use of a monoclonal antibody for the rapid purification of kidney neutral endopeptidase (\"enkephalinase\") solubilized in octyl glucoside","publication_year":1987,"publication_date":"1987-04-01","ids":{"openalex":"https://openalex.org/W2015706285","doi":"https://doi.org/10.1139/o87-050","mag":"2015706285","pmid":"https://pubmed.ncbi.nlm.nih.gov/3300709"},"language":"en","primary_location":{"id":"doi:10.1139/o87-050","doi":"https://doi.org/10.1139/o87-050","is_oa":false,"landing_page_url":"https://doi.org/10.1139/o87-050","pdf_url":null,"source":{"id":"https://openalex.org/S159551863","display_name":"Biochemistry and Cell Biology","issn_l":"0829-8211","issn":["0829-8211","1208-6002"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry and Cell Biology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086599131","display_name":"Muriel Aubry","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Muriel Aubry","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011665461","display_name":"Alfred Berteloot","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alfred Berteloot","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111380414","display_name":"Ann Beaumont","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ann Beaumont","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110015272","display_name":"Bernard Pierre Roques","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bernard P. Roques","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5074964938","display_name":"Philippe Crine","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Philippe Crine","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.2282,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.71602929,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"65","issue":"4","first_page":"398","last_page":"404"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12505","display_name":"Peptidase Inhibition and Analysis","score":0.35350000858306885,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12505","display_name":"Peptidase Inhibition and Analysis","score":0.35350000858306885,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11001","display_name":"Renin-Angiotensin System Studies","score":0.3041999936103821,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.05700000002980232,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neutral-endopeptidase","display_name":"neutral endopeptidase","score":0.9900000095367432},{"id":"https://openalex.org/keywords/brush-border-membrane","display_name":"brush border membrane","score":0.8569999933242798},{"id":"https://openalex.org/keywords/rabbit-kidney","display_name":"rabbit kidney","score":0.6940000057220459},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.8859999775886536},{"id":"https://openalex.org/keywords/immunoaffinity-purification","display_name":"immunoaffinity purification","score":0.9850000143051147},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.46000000834465027}],"concepts":[{"id":"https://openalex.org/C2780919550","wikidata":"https://www.wikidata.org/wiki/Q5379024","display_name":"Enkephalinase","level":5,"score":0.9714486598968506},{"id":"https://openalex.org/C2781372283","wikidata":"https://www.wikidata.org/wiki/Q7784695","display_name":"Thiorphan","level":4,"score":0.9189736843109131},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7484905123710632},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6937919855117798},{"id":"https://openalex.org/C2778987810","wikidata":"https://www.wikidata.org/wiki/Q7077127","display_name":"Octyl glucoside","level":3,"score":0.6080864667892456},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.5917969346046448},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.5821106433868408},{"id":"https://openalex.org/C2777774944","wikidata":"https://www.wikidata.org/wiki/Q417262","display_name":"Endopeptidase","level":3,"score":0.5691287517547607},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.4622023105621338},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.4620141088962555},{"id":"https://openalex.org/C104826730","wikidata":"https://www.wikidata.org/wiki/Q414232","display_name":"Neprilysin","level":3,"score":0.43483367562294006},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4124559760093689},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.39793020486831665},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.19836002588272095},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14798730611801147},{"id":"https://openalex.org/C2777803847","wikidata":"https://www.wikidata.org/wiki/Q325101","display_name":"Enkephalin","level":4,"score":0.13214990496635437},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.07650229334831238},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C2781063702","wikidata":"https://www.wikidata.org/wiki/Q427523","display_name":"Opioid","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005960","descriptor_name":"Glucosides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D007672","descriptor_name":"Kidney Cortex","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008871","descriptor_name":"Microvilli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014198","descriptor_name":"Trehalase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014198","descriptor_name":"Trehalase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015260","descriptor_name":"Neprilysin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1139/o87-050","doi":"https://doi.org/10.1139/o87-050","is_oa":false,"landing_page_url":"https://doi.org/10.1139/o87-050","pdf_url":null,"source":{"id":"https://openalex.org/S159551863","display_name":"Biochemistry and Cell Biology","issn_l":"0829-8211","issn":["0829-8211","1208-6002"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry and Cell Biology","raw_type":"journal-article"},{"id":"pmid:3300709","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3300709","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry and cell biology = Biochimie et biologie cellulaire","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334626","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W571837258","https://openalex.org/W1979480875","https://openalex.org/W1995518203","https://openalex.org/W2021212968","https://openalex.org/W2045296987","https://openalex.org/W2065507898"],"related_works":["https://openalex.org/W2030863679","https://openalex.org/W2045296987","https://openalex.org/W1990478684","https://openalex.org/W2047755324","https://openalex.org/W1533556186","https://openalex.org/W1543111690","https://openalex.org/W2110037172","https://openalex.org/W2418073509","https://openalex.org/W1965686429","https://openalex.org/W571837258"],"abstract_inverted_index":{"The":[0,52,99],"neutral":[1],"endopeptidase":[2],"(\"enkephalinase\")":[3],"of":[4,35,50,72],"the":[5,38,60,73],"rabbit":[6],"brush":[7,39],"border":[8,40],"membrane":[9,41],"has":[10],"been":[11],"purified":[12,100],"to":[13,78],"homogeneity":[14],"by":[15,94,103,109],"a":[16,21,32,64,114],"rapid":[17],"immunoaffinity":[18,69],"method":[19,105],"using":[20,67],"monoclonal":[22,74],"antibody.":[23],"In":[24],"contrast":[25],"with":[26,45],"other":[27,61],"methods":[28],"used":[29],"so":[30],"far,":[31],"complete":[33],"extraction":[34],"enkephalinase":[36,85],"from":[37,59],"can":[42,55,86],"be":[43,56,88],"achieved":[44],"octyl":[46],"glucoside,":[47],"without":[48],"loss":[49],"activity.":[51],"solubilized":[53],"enzyme":[54,101],"selectively":[57],"separated":[58],"proteins":[62],"in":[63,90],"single":[65,115],"step":[66],"an":[68,91],"column":[70],"consisting":[71],"antibody":[75],"covalently":[76],"linked":[77],"Sepharose":[79],"CL-4B.":[80],"It":[81],"is":[82,106],"demonstrated":[83],"that":[84],"then":[87],"recovered":[89],"active":[92],"form":[93],"elution":[95],"at":[96],"low":[97],"pH.":[98],"obtained":[102],"this":[104],"completely":[107],"inhibited":[108],"thiorphan":[110],"and":[111,125],"appears":[112],"as":[113],"94,000":[116],"dalton":[117],"protein":[118],"after":[119],"polyacrylamide":[120],"gel":[121],"electrophoresis":[122],"under":[123],"denaturing":[124],"reducing":[126],"conditions.":[127]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}