{"id":"https://openalex.org/W2015665442","doi":"https://doi.org/10.2503/jjshs1.80.295","title":"PCR Primers for Marker Assisted Backcrossing to Introduce a CTV Resistance Gene from Poncirus trifoliata (L.) Raf. into Citrus","display_name":"PCR Primers for Marker Assisted Backcrossing to Introduce a CTV Resistance Gene from Poncirus trifoliata (L.) Raf. into Citrus","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W2015665442","doi":"https://doi.org/10.2503/jjshs1.80.295","mag":"2015665442"},"language":"en","primary_location":{"id":"doi:10.2503/jjshs1.80.295","doi":"https://doi.org/10.2503/jjshs1.80.295","is_oa":true,"landing_page_url":"https://doi.org/10.2503/jjshs1.80.295","pdf_url":"https://www.jstage.jst.go.jp/article/jjshs1/80/3/80_3_295/_pdf","source":{"id":"https://openalex.org/S81704942","display_name":"Journal of the Japanese Society for Horticultural Science","issn_l":"0013-7626","issn":["0013-7626","1880-358X","1882-3351","1882-336X"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1"],"host_organization":"https://openalex.org/P4310313201","host_organization_name":"Japanese Society for Horticultural Science","host_organization_lineage":["https://openalex.org/P4310313201"],"host_organization_lineage_names":["Japanese Society for Horticultural Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the Japanese Society for Horticultural Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.jstage.jst.go.jp/article/jjshs1/80/3/80_3_295/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084260587","display_name":"Satoshi Ohta","orcid":"https://orcid.org/0000-0001-7956-7867","observed_orcids":["https://orcid.org/0000-0001-7956-7867"]},"institutions":[{"id":"https://openalex.org/I1298590031","display_name":"Shizuoka University","ror":"https://ror.org/01w6wtk13","country_code":"JP","type":"education","lineage":["https://openalex.org/I1298590031"]},{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Satoshi Ohta","raw_affiliation_strings":["Faculty of Agriculture, Shizuoka University","Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Shizuoka University","institution_ids":["https://openalex.org/I1298590031"]},{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104275716","display_name":"Tomoko Endo","orcid":"https://orcid.org/0000-0002-0142-285X","observed_orcids":["https://orcid.org/0000-0002-0142-285X"]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tomoko Endo","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101878417","display_name":"Takehiko Shimada","orcid":"https://orcid.org/0000-0002-3566-5518","observed_orcids":["https://orcid.org/0000-0002-3566-5518"]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takehiko Shimada","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061850393","display_name":"Hiroshi Fujii","orcid":"https://orcid.org/0000-0002-6710-642X","observed_orcids":["https://orcid.org/0000-0002-6710-642X"]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroshi Fujii","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057216468","display_name":"Tokuro Shimizu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tokuro Shimizu","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034464409","display_name":"Takeshi Kuniga","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takeshi Kuniga","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113950295","display_name":"Terutaka Yoshioka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Terutaka Yoshioka","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009323320","display_name":"Hirohisa Nesumi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hirohisa Nesumi","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067338993","display_name":"Toshio Yoshida","orcid":"https://orcid.org/0000-0003-0670-1513","observed_orcids":["https://orcid.org/0000-0003-0670-1513"]},"institutions":[{"id":"https://openalex.org/I4210118454","display_name":"Institute of Fruit Tree and Tea Science","ror":"https://ror.org/02fmy9v62","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I4210118454"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toshio Yoshida","raw_affiliation_strings":["Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS","institution_ids":["https://openalex.org/I4210118454"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038089230","display_name":"Mitsuo Omura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1298590031","display_name":"Shizuoka University","ror":"https://ror.org/01w6wtk13","country_code":"JP","type":"education","lineage":["https://openalex.org/I1298590031"]},{"id":"https://openalex.org/I150240954","display_name":"University of Shizuoka","ror":"https://ror.org/04rvw0k47","country_code":"JP","type":"education","lineage":["https://openalex.org/I150240954"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mitsuo Omura","raw_affiliation_strings":["Faculty of Agriculture, Shizuoka University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Shizuoka University","institution_ids":["https://openalex.org/I1298590031","https://openalex.org/I150240954"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4711,"has_fulltext":true,"cited_by_count":15,"citation_normalized_percentile":{"value":0.62702747,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"80","issue":"3","first_page":"295","last_page":"307"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.9014000296592712,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.9014000296592712,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12858","display_name":"Plant Disease Resistance and Genetics","score":0.011500000022351742,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11750","display_name":"Phytoplasmas and Hemiptera pathogens","score":0.007199999876320362,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/citrus-tristeza-virus","display_name":"citrus tristeza virus","score":0.925000011920929},{"id":"https://openalex.org/keywords/poncirus-trifoliata","display_name":"Poncirus trifoliata","score":0.9150000214576721},{"id":"https://openalex.org/keywords/marker-assisted-backcrossing","display_name":"marker-assisted backcrossing","score":0.9079999923706055},{"id":"https://openalex.org/keywords/marker-assisted-selection","display_name":"marker-assisted selection","score":0.6330000162124634},{"id":"https://openalex.org/keywords/sequence-tagged-sites","display_name":"sequence-tagged sites","score":0.7429999709129333},{"id":"https://openalex.org/keywords/linkage-groups","display_name":"linkage groups","score":0.5339999794960022},{"id":"https://openalex.org/keywords/citrus","display_name":"citrus","score":0.4490000009536743},{"id":"https://openalex.org/keywords/interspecific-hybridization","display_name":"interspecific hybridization","score":0.593999981880188},{"id":"https://openalex.org/keywords/pcr-primers","display_name":"PCR primers","score":0.7770000100135803}],"concepts":[{"id":"https://openalex.org/C72472911","wikidata":"https://www.wikidata.org/wiki/Q2004691","display_name":"Backcrossing","level":3,"score":0.7993060350418091},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7847418785095215},{"id":"https://openalex.org/C2780619479","wikidata":"https://www.wikidata.org/wiki/Q2353828","display_name":"Citrus tristeza virus","level":4,"score":0.6838561296463013},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6449629068374634},{"id":"https://openalex.org/C84597430","wikidata":"https://www.wikidata.org/wiki/Q106227","display_name":"Locus (genetics)","level":3,"score":0.6381387710571289},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5643678903579712},{"id":"https://openalex.org/C2779227213","wikidata":"https://www.wikidata.org/wiki/Q3954823","display_name":"Marker-assisted selection","level":4,"score":0.5555397868156433},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.5173491835594177},{"id":"https://openalex.org/C190789776","wikidata":"https://www.wikidata.org/wiki/Q767511","display_name":"Genetic marker","level":3,"score":0.4884447157382965},{"id":"https://openalex.org/C2777160741","wikidata":"https://www.wikidata.org/wiki/Q2311855","display_name":"Molecular marker","level":3,"score":0.45505836606025696},{"id":"https://openalex.org/C153209595","wikidata":"https://www.wikidata.org/wiki/Q501128","display_name":"Single-nucleotide polymorphism","level":4,"score":0.4538619816303253},{"id":"https://openalex.org/C119054055","wikidata":"https://www.wikidata.org/wiki/Q1576681","display_name":"Indel","level":5,"score":0.4292849898338318},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.2636643946170807},{"id":"https://openalex.org/C109110057","wikidata":"https://www.wikidata.org/wiki/Q1495434","display_name":"Plant virus","level":3,"score":0.1897016167640686},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.13609102368354797}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.2503/jjshs1.80.295","doi":"https://doi.org/10.2503/jjshs1.80.295","is_oa":true,"landing_page_url":"https://doi.org/10.2503/jjshs1.80.295","pdf_url":"https://www.jstage.jst.go.jp/article/jjshs1/80/3/80_3_295/_pdf","source":{"id":"https://openalex.org/S81704942","display_name":"Journal of the Japanese Society for Horticultural Science","issn_l":"0013-7626","issn":["0013-7626","1880-358X","1882-3351","1882-336X"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1"],"host_organization":"https://openalex.org/P4310313201","host_organization_name":"Japanese Society for Horticultural Science","host_organization_lineage":["https://openalex.org/P4310313201"],"host_organization_lineage_names":["Japanese Society for Horticultural Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the Japanese Society for Horticultural Science","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.2503/jjshs1.80.295","doi":"https://doi.org/10.2503/jjshs1.80.295","is_oa":true,"landing_page_url":"https://doi.org/10.2503/jjshs1.80.295","pdf_url":"https://www.jstage.jst.go.jp/article/jjshs1/80/3/80_3_295/_pdf","source":{"id":"https://openalex.org/S81704942","display_name":"Journal of the Japanese Society for Horticultural Science","issn_l":"0013-7626","issn":["0013-7626","1880-358X","1882-3351","1882-336X"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1"],"host_organization":"https://openalex.org/P4310313201","host_organization_name":"Japanese Society for Horticultural Science","host_organization_lineage":["https://openalex.org/P4310313201"],"host_organization_lineage_names":["Japanese Society for Horticultural Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the Japanese Society for Horticultural Science","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.9208999872207642}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320324213","display_name":"National Agriculture and Food Research Organization","ror":"https://ror.org/023v4bd62"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2015665442.pdf","grobid_xml":"https://content.openalex.org/works/W2015665442.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W1887623669","https://openalex.org/W1976162379","https://openalex.org/W2000303981","https://openalex.org/W2006807779","https://openalex.org/W2047661597","https://openalex.org/W2082514673","https://openalex.org/W2094169637","https://openalex.org/W2102651075","https://openalex.org/W2140130430","https://openalex.org/W2183768863","https://openalex.org/W2285044276","https://openalex.org/W2330675847","https://openalex.org/W2557094793","https://openalex.org/W2714492473","https://openalex.org/W2767323734","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2585178772","https://openalex.org/W1887623669","https://openalex.org/W1986262552","https://openalex.org/W2000303981","https://openalex.org/W2013767214","https://openalex.org/W2047661597","https://openalex.org/W2087615297","https://openalex.org/W2043139792","https://openalex.org/W282416724","https://openalex.org/W1976162379"],"abstract_inverted_index":{"Citrus":[0,137],"tristeza":[1],"virus":[2],"(CTV)":[3],"is":[4,156],"an":[5],"acute":[6],"pathogen":[7],"that":[8,172],"causes":[9],"serious":[10],"damage":[11],"to":[12,40,49,68,71,76,111,117,178,205],"the":[13,42,112,140,152,160,165,168,186,207],"citrus":[14],"industry.":[15],"Poncirus":[16],"trifoliata":[17,48,129,181,209],"(L.)":[18],"Raf.,":[19],"a":[20,29,91],"sexually":[21],"compatible":[22],"species":[23],"with":[24,120],"Citrus,":[25,50],"has":[26,55],"resistance":[27,44,70,80,154,211],"against":[28],"broad":[30],"range":[31],"of":[32,90,135,167,191],"CTV":[33,43,69,79,85,118,153,210],"strains.":[34],"Breeding":[35],"programs":[36],"have":[37],"been":[38,57],"conducted":[39],"introduce":[41,78,206],"gene":[45,212],"from":[46,133],"P.":[47,128,180,208],"but":[51],"no":[52],"commercial":[53],"cultivar":[54],"yet":[56],"developed.":[58],"In":[59],"this":[60,196],"study,":[61],"we":[62],"developed":[63,126,194],"four":[64,84,107],"selection":[65,75],"markers":[66,87,108,132,163,174],"linked":[67,116],"enable":[72],"marker":[73,97,202],"assisted":[74,203],"efficiently":[77],"into":[81,213],"Citrus.":[82,214],"The":[83,189],"resistance-linked":[86],"were":[88,109,115,144],"composed":[89],"co-dominant":[92],"single":[93],"nucleotide":[94],"polymorphism":[95],"(SNP)":[96],"and":[98,114],"three":[99],"dominant":[100],"sequence":[101],"tagged":[102],"site":[103],"(STS)":[104],"markers.":[105],"All":[106],"fitted":[110],"progeny":[113],"resistance,":[119],"only":[121],"2.8%":[122],"exceptions.":[123],"We":[124,158],"also":[125],"46":[127,141],"allele":[130],"identification":[131],"alleles":[134],"35":[136],"species.":[138],"Among":[139],"markers,":[142],"nine":[143],"located":[145,159],"in":[146,185,195],"linkage":[147,169],"group":[148],"2,":[149],"on":[150,164],"which":[151],"locus":[155],"located.":[157],"other":[161],"31":[162],"rest":[166],"groups":[170],"so":[171],"these":[173],"could":[175],"be":[176,199],"used":[177],"distinguish":[179],"genome":[182],"regions":[183],"remaining":[184],"hybrid":[187],"progenies.":[188],"set":[190],"PCR":[192],"primers":[193],"study":[197],"will":[198],"useful":[200],"for":[201],"backcrossing":[204]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}