{"id":"https://openalex.org/W2015538396","doi":"https://doi.org/10.1267/ahc.30.309","title":"Nuclear Staining for Laser Confocal Microscopy.","display_name":"Nuclear Staining for Laser Confocal Microscopy.","publication_year":1997,"publication_date":"1997-01-01","ids":{"openalex":"https://openalex.org/W2015538396","doi":"https://doi.org/10.1267/ahc.30.309","mag":"2015538396"},"language":"en","primary_location":{"id":"doi:10.1267/ahc.30.309","doi":"https://doi.org/10.1267/ahc.30.309","is_oa":true,"landing_page_url":"https://doi.org/10.1267/ahc.30.309","pdf_url":"https://www.jstage.jst.go.jp/article/ahc1968/30/3/30_3_309/_pdf","source":{"id":"https://openalex.org/S151127207","display_name":"ACTA HISTOCHEMICA ET CYTOCHEMICA","issn_l":"0044-5991","issn":["0044-5991","1347-5800"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800631","host_organization_name":"Japan Society of Histochemistry and Cytochemistry","host_organization_lineage":["https://openalex.org/P4320800631"],"host_organization_lineage_names":["Japan Society of Histochemistry and Cytochemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACTA HISTOCHEMICA ET CYTOCHEMICA","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jstage.jst.go.jp/article/ahc1968/30/3/30_3_309/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070289694","display_name":"Toshiyuki Matsuzaki","orcid":"https://orcid.org/0000-0003-3512-6810","observed_orcids":["https://orcid.org/0000-0003-3512-6810"]},"institutions":[{"id":"https://openalex.org/I165735259","display_name":"Gunma University","ror":"https://ror.org/046fm7598","country_code":"JP","type":"education","lineage":["https://openalex.org/I165735259"]},{"id":"https://openalex.org/I4405286117","display_name":"Institute for Molecular and Cellular Regulation, Gunma University","ror":"https://ror.org/015vdva42","country_code":"JP","type":"facility","lineage":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toshiyuki Matsuzaki","raw_affiliation_strings":["Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University","institution_ids":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101484992","display_name":"Takeshi Suzuki","orcid":"https://orcid.org/0000-0001-8930-5895","observed_orcids":["https://orcid.org/0000-0001-8930-5895"]},"institutions":[{"id":"https://openalex.org/I165735259","display_name":"Gunma University","ror":"https://ror.org/046fm7598","country_code":"JP","type":"education","lineage":["https://openalex.org/I165735259"]},{"id":"https://openalex.org/I4405286117","display_name":"Institute for Molecular and Cellular Regulation, Gunma University","ror":"https://ror.org/015vdva42","country_code":"JP","type":"facility","lineage":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takeshi Suzuki","raw_affiliation_strings":["Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University","institution_ids":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109854003","display_name":"Keiko Fujikura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165735259","display_name":"Gunma University","ror":"https://ror.org/046fm7598","country_code":"JP","type":"education","lineage":["https://openalex.org/I165735259"]},{"id":"https://openalex.org/I4405286117","display_name":"Institute for Molecular and Cellular Regulation, Gunma University","ror":"https://ror.org/015vdva42","country_code":"JP","type":"facility","lineage":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Keiko Fujikura","raw_affiliation_strings":["Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University","institution_ids":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5107415005","display_name":"Kuniaki Takata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165735259","display_name":"Gunma University","ror":"https://ror.org/046fm7598","country_code":"JP","type":"education","lineage":["https://openalex.org/I165735259"]},{"id":"https://openalex.org/I4405286117","display_name":"Institute for Molecular and Cellular Regulation, Gunma University","ror":"https://ror.org/015vdva42","country_code":"JP","type":"facility","lineage":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kuniaki Takata","raw_affiliation_strings":["Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University","institution_ids":["https://openalex.org/I165735259","https://openalex.org/I4405286117"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.7948,"has_fulltext":true,"cited_by_count":37,"citation_normalized_percentile":{"value":0.90730932,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"30","issue":"3","first_page":"309","last_page":"314"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.5928000211715698,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.5928000211715698,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13921","display_name":"Biological Stains and Phytochemicals","score":0.19580000638961792,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.17319999635219574,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nuclear-staining","display_name":"nuclear staining","score":0.7860000133514404},{"id":"https://openalex.org/keywords/dna-staining","display_name":"DNA staining","score":0.5740000009536743},{"id":"https://openalex.org/keywords/yo-pro-1","display_name":"YO-PRO-1","score":0.8500000238418579},{"id":"https://openalex.org/keywords/sytox-green","display_name":"SYTOX Green","score":0.8149999976158142},{"id":"https://openalex.org/keywords/sybr-green-i","display_name":"SYBR Green I","score":0.9490000009536743},{"id":"https://openalex.org/keywords/propidium-iodide","display_name":"propidium iodide","score":0.9179999828338623},{"id":"https://openalex.org/keywords/picogreen","display_name":"PicoGreen","score":0.699999988079071},{"id":"https://openalex.org/keywords/fluorescence-bleaching","display_name":"fluorescence bleaching","score":0.6930000185966492},{"id":"https://openalex.org/keywords/cytoplasmic-rna","display_name":"cytoplasmic RNA","score":0.6309999823570251},{"id":"https://openalex.org/keywords/nucleolar-rna","display_name":"nucleolar RNA","score":0.8370000123977661},{"id":"https://openalex.org/keywords/dye-screening","display_name":"dye screening","score":0.5669999718666077}],"concepts":[{"id":"https://openalex.org/C2775934118","wikidata":"https://www.wikidata.org/wiki/Q424169","display_name":"Propidium iodide","level":4,"score":0.9281013011932373},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.8590124249458313},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6557598114013672},{"id":"https://openalex.org/C115960442","wikidata":"https://www.wikidata.org/wiki/Q769863","display_name":"Nuclear DNA","level":4,"score":0.64883953332901},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.6372902989387512},{"id":"https://openalex.org/C2780968160","wikidata":"https://www.wikidata.org/wiki/Q423692","display_name":"SYBR Green I","level":4,"score":0.5465788245201111},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5459846258163452},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5422696471214294},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.48731908202171326},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4784103333950043},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4260393977165222},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28976643085479736},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.18563717603683472},{"id":"https://openalex.org/C24586158","wikidata":"https://www.wikidata.org/wiki/Q27075","display_name":"Mitochondrial DNA","level":3,"score":0.12632343173027039},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07130753993988037},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05296391248703003},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1267/ahc.30.309","doi":"https://doi.org/10.1267/ahc.30.309","is_oa":true,"landing_page_url":"https://doi.org/10.1267/ahc.30.309","pdf_url":"https://www.jstage.jst.go.jp/article/ahc1968/30/3/30_3_309/_pdf","source":{"id":"https://openalex.org/S151127207","display_name":"ACTA HISTOCHEMICA ET CYTOCHEMICA","issn_l":"0044-5991","issn":["0044-5991","1347-5800"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800631","host_organization_name":"Japan Society of Histochemistry and Cytochemistry","host_organization_lineage":["https://openalex.org/P4320800631"],"host_organization_lineage_names":["Japan Society of Histochemistry and Cytochemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACTA HISTOCHEMICA ET CYTOCHEMICA","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1267/ahc.30.309","doi":"https://doi.org/10.1267/ahc.30.309","is_oa":true,"landing_page_url":"https://doi.org/10.1267/ahc.30.309","pdf_url":"https://www.jstage.jst.go.jp/article/ahc1968/30/3/30_3_309/_pdf","source":{"id":"https://openalex.org/S151127207","display_name":"ACTA HISTOCHEMICA ET CYTOCHEMICA","issn_l":"0044-5991","issn":["0044-5991","1347-5800"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800631","host_organization_name":"Japan Society of Histochemistry and Cytochemistry","host_organization_lineage":["https://openalex.org/P4320800631"],"host_organization_lineage_names":["Japan Society of Histochemistry and Cytochemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACTA HISTOCHEMICA ET CYTOCHEMICA","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2015538396.pdf","grobid_xml":"https://content.openalex.org/works/W2015538396.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W68695562","https://openalex.org/W800515351","https://openalex.org/W1500863317","https://openalex.org/W1606920154","https://openalex.org/W1644507360","https://openalex.org/W1971909907","https://openalex.org/W2001213773","https://openalex.org/W2014410623","https://openalex.org/W2016062690","https://openalex.org/W2024819037","https://openalex.org/W2030306616","https://openalex.org/W2048212315","https://openalex.org/W2052526975","https://openalex.org/W2065842764","https://openalex.org/W2074729278","https://openalex.org/W2076302815","https://openalex.org/W2091674644","https://openalex.org/W2115276470","https://openalex.org/W2115458875","https://openalex.org/W2131207346","https://openalex.org/W2442803553","https://openalex.org/W4235171237"],"related_works":["https://openalex.org/W2131207346","https://openalex.org/W2325772161","https://openalex.org/W2038070685","https://openalex.org/W2016062690","https://openalex.org/W2012157299","https://openalex.org/W2160921380","https://openalex.org/W1921113654","https://openalex.org/W1969753795","https://openalex.org/W205982582","https://openalex.org/W2072389503"],"abstract_inverted_index":{"Twenty":[0],"nucleic":[1,72],"acid-specific":[2],"fluorescent":[3,99],"dyes":[4,165],"were":[5,42,89],"screened":[6],"for":[7,45,91,152],"use":[8],"in":[9,12,49,69],"nuclear":[10,47,92,117,128,162],"staining":[11,48,74,132,164],"laser":[13],"confocal":[14],"microscopy.":[15],"Eleven":[16],"of":[17,71,133,161],"them,":[18],"BO-PRO-1,":[19],"YO-PRO-1,":[20,82],"SYBR":[21,86],"Green":[22,87,111],"I,":[23],"PicoGreen,":[24],"SYTOX":[25,83,110],"Green,":[26,84],"TO-PRO-1,":[27],"POPO-3,":[28],"propidium":[29,125],"iodide,":[30],"YO-PRO-3,":[31],"TOTO-3,":[32],"and":[33,65,85,107,113,137,176],"TO-PRO-3":[34,145],"gave":[35],"sufficient":[36],"fluorescence":[37,63,156,172],"by":[38,169],"in-gel":[39],"assay.":[40],"They":[41],"also":[43],"tested":[44],"the":[46,62,67,97,105,116,171],"cultured":[50],"cells.":[51],"When":[52],"glycerol":[53],"was":[54,150,157],"used":[55],"as":[56],"a":[57],"mounting":[58],"medium,":[59],"it":[60,102],"enhanced":[61],"intensity":[64],"retarded":[66],"bleaching":[68,177],"most":[70],"acid":[73],"dyes.":[75],"Among":[76,119],"green":[77],"fluorochromes":[78,121],"excited":[79,122,146],"at":[80,123,147],"488nm,":[81],"I":[88],"suitable":[90],"DNA":[93,129,140,163,174],"staining.":[94],"YO-PRO-1":[95],"showed":[96],"strongest":[98],"intensity,":[100,173],"but":[101,141,154],"weakly":[103],"co-stained":[104],"cytoplasmic":[106],"nucleolar":[108],"RNAs.":[109],"strongly":[112,136],"specifically":[114,138],"stained":[115,127,139],"DNA.":[118],"red":[120],"568nm,":[124],"iodide":[126],"with":[130],"simultaneous":[131],"RNA.":[134],"YO-PRO-3":[135],"bleached":[142,158],"relatively":[143],"rapidly.":[144,159],"647":[148],"nm":[149],"specific":[151],"DNA,":[153],"its":[155],"Selection":[160],"should":[166],"be":[167],"done":[168],"considering":[170],"specificity,":[175],"characteristics.":[178]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}