{"id":"https://openalex.org/W2015436734","doi":"https://doi.org/10.1159/000260601","title":"Preparation of Partially Decalcified Sections of Human Dental Enamel for Electron Microscopy","display_name":"Preparation of Partially Decalcified Sections of Human Dental Enamel for Electron Microscopy","publication_year":2009,"publication_date":"2009-11-17","ids":{"openalex":"https://openalex.org/W2015436734","doi":"https://doi.org/10.1159/000260601","mag":"2015436734","pmid":"https://pubmed.ncbi.nlm.nih.gov/7046938"},"language":"en","primary_location":{"id":"doi:10.1159/000260601","doi":"https://doi.org/10.1159/000260601","is_oa":false,"landing_page_url":"https://doi.org/10.1159/000260601","pdf_url":null,"source":{"id":"https://openalex.org/S23327923","display_name":"Caries Research","issn_l":"0008-6568","issn":["0008-6568","1421-976X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Caries Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110537400","display_name":"Robert S. B. Liem","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"R.S.B. Liem","raw_affiliation_strings":["Department of Oral Biology, School of Dentistry, University of Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral Biology, School of Dentistry, University of Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113688031","display_name":"H.W.B. Jansen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"H.W.B. Jansen","raw_affiliation_strings":["Department of Oral Biology, School of Dentistry, University of Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral Biology, School of Dentistry, University of Groningen, The Netherlands","institution_ids":["https://openalex.org/I169381384"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I169381384"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.15931841,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"16","issue":"3","first_page":"217","last_page":"226"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10113","display_name":"Dental materials and restorations","score":0.6460000276565552,"subfield":{"id":"https://openalex.org/subfields/3500","display_name":"General Dentistry"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10113","display_name":"Dental materials and restorations","score":0.6460000276565552,"subfield":{"id":"https://openalex.org/subfields/3500","display_name":"General Dentistry"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.08550000190734863,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10368","display_name":"Dental Health and Care Utilization","score":0.07129999995231628,"subfield":{"id":"https://openalex.org/subfields/3500","display_name":"General Dentistry"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dental-enamel","display_name":"dental enamel","score":0.9309999942779541},{"id":"https://openalex.org/keywords/carious-enamel","display_name":"carious enamel","score":0.6340000033378601},{"id":"https://openalex.org/keywords/ultrathin-sections","display_name":"ultrathin sections","score":0.8090000152587891},{"id":"https://openalex.org/keywords/serial-sectioning","display_name":"serial sectioning","score":0.656000018119812},{"id":"https://openalex.org/keywords/post-fixation","display_name":"post-fixation","score":0.49399998784065247}],"concepts":[{"id":"https://openalex.org/C26905121","wikidata":"https://www.wikidata.org/wiki/Q4941547","display_name":"Bone decalcification","level":2,"score":0.9804500341415405},{"id":"https://openalex.org/C156887251","wikidata":"https://www.wikidata.org/wiki/Q2386424","display_name":"Enamel paint","level":2,"score":0.8846491575241089},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.5295225977897644},{"id":"https://openalex.org/C106487976","wikidata":"https://www.wikidata.org/wiki/Q685816","display_name":"Matrix (chemical analysis)","level":2,"score":0.5278886556625366},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.5267505049705505},{"id":"https://openalex.org/C2781112085","wikidata":"https://www.wikidata.org/wiki/Q561","display_name":"Human tooth","level":3,"score":0.4993245601654053},{"id":"https://openalex.org/C26771246","wikidata":"https://www.wikidata.org/wiki/Q321095","display_name":"Scanning electron microscope","level":2,"score":0.4951387941837311},{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.4570581614971161},{"id":"https://openalex.org/C199343813","wikidata":"https://www.wikidata.org/wiki/Q12128","display_name":"Dentistry","level":1,"score":0.40863093733787537},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3714140057563782},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.2002376914024353},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.11827036738395691},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.09505796432495117},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.05083248019218445},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D003461","descriptor_name":"Crystallography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003648","descriptor_name":"Decalcification Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003731","descriptor_name":"Dental Caries","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D003743","descriptor_name":"Dental Enamel","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D003773","descriptor_name":"Dental Plaque","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008867","descriptor_name":"Microtomy","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013048","descriptor_name":"Specimen Handling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1159/000260601","doi":"https://doi.org/10.1159/000260601","is_oa":false,"landing_page_url":"https://doi.org/10.1159/000260601","pdf_url":null,"source":{"id":"https://openalex.org/S23327923","display_name":"Caries Research","issn_l":"0008-6568","issn":["0008-6568","1421-976X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Caries Research","raw_type":"journal-article"},{"id":"pmid:7046938","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7046938","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Caries research","raw_type":"Journal Article"},{"id":"pmh:oai:pure.rug.nl:openaire_cris_publications/481b574f-f6ff-49b0-a23f-157a480e47b0","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/11370/481b574f-f6ff-49b0-a23f-157a480e47b0","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Liem, R S B & Jansen, H W B 1982, 'Preparation of Partially Decalcified Sections of Human Dental Enamel for Electron Microscopy', Caries Research, vol. 16, no. 3, pp. 217-226. https://doi.org/10.1159/000260601","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:rug:oai:pure.rug.nl:publications/481b574f-f6ff-49b0-a23f-157a480e47b0","doi":null,"is_oa":false,"landing_page_url":"https://research.rug.nl/en/publications/481b574f-f6ff-49b0-a23f-157a480e47b0","pdf_url":null,"source":{"id":"https://openalex.org/S4306400420","display_name":"University of Groningen research database (University of Groningen / Centre for Information Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169381384","host_organization_name":"University of Groningen","host_organization_lineage":["https://openalex.org/I169381384"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"CARIES RESEARCH, 16(3), 217 - 226. KARGER","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2012566522","https://openalex.org/W2409117400","https://openalex.org/W2414928884","https://openalex.org/W2040596110","https://openalex.org/W2470492588","https://openalex.org/W2412683095","https://openalex.org/W2412045718","https://openalex.org/W2029664116","https://openalex.org/W1997697941","https://openalex.org/W1969360269"],"abstract_inverted_index":{"A":[0],"simple":[1],"method":[2,104],"for":[3,17],"the":[4,66,86,106,112],"preparation":[5,113],"of":[6,10,33,54,76,93,98,114],"partially":[7],"decalcified":[8],"sections":[9,29,37,100],"sound":[11],"and":[12,62,84,95,110],"carious":[13],"human":[14,24],"dental":[15],"enamel":[16,87,107],"electron":[18],"microscopic":[19],"studies":[20],"is":[21,72],"described.":[22],"Formalin-fixed":[23],"teeth":[25],"are":[26,38,101],"sawed":[27],"in":[28,40,50,65],"with":[30],"a":[31],"thickness":[32],"0.5":[34],"mm.":[35],"These":[36],"embedded":[39],"Poly":[41],"Bed":[42],"812":[43],"embedding":[44],"medium":[45],"prior":[46],"to":[47],"decalcification,":[48],"immersed":[49],"an":[51],"aqueous":[52],"solution":[53],"1%":[55],"(w/v)":[56],"chromic":[57],"potassium":[58,81],"sulphate,":[59],"pH":[60],"3.4":[61],"subsequently":[63],"reembedded":[64],"same":[67],"resin.":[68],"The":[69,103],"decalcification":[70],"process":[71],"checked":[73],"by":[74],"means":[75],"transmitted":[77],"light":[78],"microscopy.":[79],"Chromic":[80],"surphate":[82],"fixes":[83],"contrasts":[85],"matrix":[88,108],"structures":[89],"so":[90],"that":[91],"post-fixing":[92],"specimens":[94],"additional":[96],"staining":[97],"ultrathin":[99,116],"unnecessary.":[102],"preserves":[105],"well":[109],"permits":[111],"serial":[115],"sections.":[117]},"counts_by_year":[{"year":2023,"cited_by_count":9},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}