{"id":"https://openalex.org/W2015426747","doi":"https://doi.org/10.1002/glia.440050203","title":"Spontaneous changes in intracellular calcium concentration in type I astrocytes from rat cerebral cortex in primary culture","display_name":"Spontaneous changes in intracellular calcium concentration in type I astrocytes from rat cerebral cortex in primary culture","publication_year":1992,"publication_date":"1992-01-01","ids":{"openalex":"https://openalex.org/W2015426747","doi":"https://doi.org/10.1002/glia.440050203","mag":"2015426747","pmid":"https://pubmed.ncbi.nlm.nih.gov/1349589"},"language":"en","primary_location":{"id":"doi:10.1002/glia.440050203","doi":"https://doi.org/10.1002/glia.440050203","is_oa":false,"landing_page_url":"https://doi.org/10.1002/glia.440050203","pdf_url":null,"source":{"id":"https://openalex.org/S147407642","display_name":"Glia","issn_l":"0894-1491","issn":["0894-1491","1098-1136"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Glia","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021021049","display_name":"Alessandro Fatatis","orcid":"https://orcid.org/0000-0003-3945-8513","observed_orcids":["https://orcid.org/0000-0003-3945-8513"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210144228","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alessandro Fatatis","raw_affiliation_strings":["Laboratory of Developmental Neurobiology, National Institutes of Child Health and Human Development, NIH, Bethesda, MD 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Developmental Neurobiology, National Institutes of Child Health and Human Development, NIH, Bethesda, MD 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113977290","display_name":"James T. Russell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210144228","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"James T. Russell","raw_affiliation_strings":["Section of Neuronal Secretory Systems, Laboratory of Developmental Neurobiology, National Institutes of Child Health and Human Development, Bethesda, Maryland 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section of Neuronal Secretory Systems, Laboratory of Developmental Neurobiology, National Institutes of Child Health and Human Development, Bethesda, Maryland 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5113977290"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210144228"],"apc_list":{"value":5450,"currency":"USD","value_usd":5450},"apc_paid":null,"fwci":2.9107,"has_fulltext":false,"cited_by_count":81,"citation_normalized_percentile":{"value":0.92668876,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"5","issue":"2","first_page":"95","last_page":"104"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.4641000032424927,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.4641000032424927,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.24770000576972961,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11204","display_name":"Ion Channels and Receptors","score":0.10199999809265137,"subfield":{"id":"https://openalex.org/subfields/2809","display_name":"Sensory Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/astrocytes","display_name":"astrocytes","score":0.8360000252723694},{"id":"https://openalex.org/keywords/intracellular-calcium","display_name":"intracellular calcium","score":0.8970000147819519},{"id":"https://openalex.org/keywords/calcium-oscillations","display_name":"calcium oscillations","score":0.6980000138282776},{"id":"https://openalex.org/keywords/cerebral-cortex","display_name":"cerebral cortex","score":0.781000018119812},{"id":"https://openalex.org/keywords/fura-2-fluorescence","display_name":"fura-2 fluorescence","score":0.7229999899864197},{"id":"https://openalex.org/keywords/depolarization-induced-calcium-release","display_name":"depolarization-induced calcium release","score":0.7760000228881836},{"id":"https://openalex.org/keywords/voltage-gated-calcium-channels","display_name":"voltage-gated calcium channels","score":0.7429999709129333},{"id":"https://openalex.org/keywords/glutamate","display_name":"glutamate","score":0.8100000023841858},{"id":"https://openalex.org/keywords/extracellular-calcium","display_name":"extracellular calcium","score":0.6629999876022339},{"id":"https://openalex.org/keywords/tetrodotoxin","display_name":"tetrodotoxin","score":0.7139999866485596},{"id":"https://openalex.org/keywords/ryanodine","display_name":"ryanodine","score":0.7139999866485596},{"id":"https://openalex.org/keywords/sarcoplasmic-reticulum-calcium-release","display_name":"sarcoplasmic reticulum calcium release","score":0.796999990940094}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8496561646461487},{"id":"https://openalex.org/C2781041448","wikidata":"https://www.wikidata.org/wiki/Q75839","display_name":"Cerebral cortex","level":2,"score":0.6853476166725159},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.6310511231422424},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.5855472087860107},{"id":"https://openalex.org/C2777542381","wikidata":"https://www.wikidata.org/wiki/Q502961","display_name":"Astrocyte","level":3,"score":0.5846127867698669},{"id":"https://openalex.org/C181080969","wikidata":"https://www.wikidata.org/wiki/Q60097","display_name":"Calcium in biology","level":3,"score":0.5812262296676636},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.5432350635528564},{"id":"https://openalex.org/C2780884517","wikidata":"https://www.wikidata.org/wiki/Q177105","display_name":"Neuroglia","level":3,"score":0.5391054749488831},{"id":"https://openalex.org/C2994367422","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Primary culture","level":3,"score":0.522678792476654},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.31443631649017334},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.27154573798179626},{"id":"https://openalex.org/C529278444","wikidata":"https://www.wikidata.org/wiki/Q47273","display_name":"Central nervous system","level":2,"score":0.24452084302902222},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.17604020237922668},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07653003931045532},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001253","descriptor_name":"Astrocytes","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D001253","descriptor_name":"Astrocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002540","descriptor_name":"Cerebral Cortex","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D005971","descriptor_name":"Glutamates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008564","descriptor_name":"Membrane Potentials","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009498","descriptor_name":"Neurotoxins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D015220","descriptor_name":"Calcium Channels","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D016257","descriptor_name":"Fura-2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018674","descriptor_name":"Adrenergic Antagonists","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/glia.440050203","doi":"https://doi.org/10.1002/glia.440050203","is_oa":false,"landing_page_url":"https://doi.org/10.1002/glia.440050203","pdf_url":null,"source":{"id":"https://openalex.org/S147407642","display_name":"Glia","issn_l":"0894-1491","issn":["0894-1491","1098-1136"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Glia","raw_type":"journal-article"},{"id":"pmid:1349589","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1349589","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Glia","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W656714662","https://openalex.org/W1572564124","https://openalex.org/W1597577457","https://openalex.org/W1825752926","https://openalex.org/W1964845842","https://openalex.org/W1968070701","https://openalex.org/W1969568346","https://openalex.org/W1987391644","https://openalex.org/W2004589688","https://openalex.org/W2007884285","https://openalex.org/W2009421992","https://openalex.org/W2031785864","https://openalex.org/W2034038906","https://openalex.org/W2035106224","https://openalex.org/W2041416738","https://openalex.org/W2050251438","https://openalex.org/W2059994807","https://openalex.org/W2065556778","https://openalex.org/W2071378425","https://openalex.org/W2075224347","https://openalex.org/W2081430450","https://openalex.org/W2082203098","https://openalex.org/W2085726430","https://openalex.org/W2088233139","https://openalex.org/W2090971379","https://openalex.org/W2091883621","https://openalex.org/W2093182332","https://openalex.org/W2093663221","https://openalex.org/W2120772267","https://openalex.org/W2130793535","https://openalex.org/W2136632189","https://openalex.org/W2141260882","https://openalex.org/W2143876981","https://openalex.org/W2153158525","https://openalex.org/W2155298124","https://openalex.org/W2481327068","https://openalex.org/W4233150381","https://openalex.org/W4238401914","https://openalex.org/W4300720602","https://openalex.org/W7074137024"],"related_works":["https://openalex.org/W1999389122","https://openalex.org/W2572248955","https://openalex.org/W2133346716","https://openalex.org/W3144487059","https://openalex.org/W2001159446","https://openalex.org/W7095806649","https://openalex.org/W2952766587","https://openalex.org/W2091441979","https://openalex.org/W2118626931","https://openalex.org/W1965529878"],"abstract_inverted_index":{"Measurement":[0],"of":[1,31,33,99,113,124,182],"fura-2":[2],"fluorescence":[3],"in":[4,49,57,94,138,142,151,161,165,196,205,224,226],"type":[5],"I":[6],"astrocytes":[7,101,227],"from":[8,28,144,230],"rat":[9],"cerebral":[10],"cortex":[11],"showed":[12],"that":[13,67,88,198,221],"the":[14,77,89,92,100,107,125,212],"intracellular":[15,152,166],"calcium":[16,32,79,115,129,153,167,184,187],"ion":[17],"concentration":[18,80],"undergoes":[19],"very":[20],"large":[21,194],"spontaneous":[22,73,108,213,222],"changes.":[23,236],"These":[24,136],"spike-like":[25],"changes":[26,137,223],"ranged":[27],"resting":[29,173],"levels":[30,86],"50-250":[34],"nM":[35],"to":[36,46,83,171,180],"as":[37,39,64],"high":[38],"1-2":[40],"microM.":[41],"The":[42,60,72,208],"spikes":[43,74,214],"were":[44,54,140,199],"found":[45],"be":[47],"irregular":[48],"frequency":[50],"and":[51,53,119,148,175,185,232],"amplitude":[52],"frequently":[55],"synchronous":[56,61],"confluent":[58],"cultures.":[59],"events":[62],"appeared":[63],"propagating":[65],"waves":[66],"spread":[68],"over":[69],"many":[70],"cells.":[71,207],"persisted":[75],"when":[76],"extracellular":[78,183],"was":[81,96,132,178],"reduced":[82],"below":[84],"micromolar":[85],"suggesting":[87],"source":[90],"for":[91,211],"increases":[93,150,195],"[Ca2+]i":[95,139,197,225],"intracellular.":[97],"Treatment":[98],"with":[102,117,201],"tetrodotoxin":[103],"did":[104,111],"not":[105,217],"abolish":[106],"changes,":[109],"nor":[110],"blockade":[112],"voltage-dependent":[114],"channels":[116],"nimodipine":[118],"D-600.":[120],"Ryanodine,":[121],"a":[122,162],"blocker":[123],"sarcoplasmic":[126],"reticulum":[127],"calcium-induced":[128],"release":[130],"channel,":[131],"also":[133],"without":[134],"effect.":[135],"different":[141],"character":[143],"both":[145],"agonist-induced":[146,231],"oscillations":[147,204],"depolarization-induced":[149,235],"concentration.":[154],"Depolarization":[155],"using":[156],"25-100":[157],"mM":[158],"[K+]o":[159],"resulted":[160],"prompt":[163],"rise":[164],"concentration,":[168],"which":[169],"returned":[170],"near":[172],"levels,":[174],"this":[176],"response":[177],"sensitive":[179],"removal":[181],"voltage-gated":[186],"channel":[188],"antagonists.":[189],"L-glutamate":[190],"(0.5-100":[191],"microM)":[192],"caused":[193],"associated":[200],"discrete":[202],"periodic":[203],"some":[206],"cellular":[209],"trigger":[210],"is":[215],"currently":[216],"understood.":[218],"We":[219],"conclude":[220],"are":[228],"distinct":[229],"membrane":[233],"potential":[234]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2016-06-24T00:00:00"}