{"id":"https://openalex.org/W2015329331","doi":"https://doi.org/10.1038/nprot.2012.022","title":"Highly multiplexed and strand-specific single-cell RNA 5\u2032 end sequencing","display_name":"Highly multiplexed and strand-specific single-cell RNA 5\u2032 end sequencing","publication_year":2012,"publication_date":"2012-04-05","ids":{"openalex":"https://openalex.org/W2015329331","doi":"https://doi.org/10.1038/nprot.2012.022","mag":"2015329331","pmid":"https://pubmed.ncbi.nlm.nih.gov/22481528"},"language":"en","primary_location":{"id":"doi:10.1038/nprot.2012.022","doi":"https://doi.org/10.1038/nprot.2012.022","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nprot.2012.022","pdf_url":null,"source":{"id":"https://openalex.org/S109387254","display_name":"Nature Protocols","issn_l":"1750-2799","issn":["1750-2799","1754-2189"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Protocols","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100637957","display_name":"Saiful Islam","orcid":"https://orcid.org/0000-0001-5777-9164","observed_orcids":["https://orcid.org/0000-0001-5777-9164"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Saiful Islam","raw_affiliation_strings":["Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052948245","display_name":"Una Kj\u00e4llquist","orcid":"https://orcid.org/0000-0002-8123-9261","observed_orcids":["https://orcid.org/0000-0002-8123-9261"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Una Kj\u00e4llquist","raw_affiliation_strings":["Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024382910","display_name":"Annalena Moliner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Annalena Moliner","raw_affiliation_strings":["Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102100258","display_name":"Pawe\u0142 Zaj\u0105c","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Pawel Zajac","raw_affiliation_strings":["Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042998865","display_name":"Jian\u2010Bing Fan","orcid":"https://orcid.org/0000-0002-7852-8097","observed_orcids":["https://orcid.org/0000-0002-7852-8097"]},"institutions":[{"id":"https://openalex.org/I178645029","display_name":"Illumina (United States)","ror":"https://ror.org/05k34t975","country_code":"US","type":"company","lineage":["https://openalex.org/I178645029"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jian-Bing Fan","raw_affiliation_strings":["Illumina Inc., San Diego, California, USA","Illumina Incorporated, San Diego, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Illumina Inc., San Diego, California, USA","institution_ids":["https://openalex.org/I178645029"]},{"raw_affiliation_string":"Illumina Incorporated, San Diego, USA","institution_ids":["https://openalex.org/I178645029"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087224315","display_name":"Peter L\u00f6nnerberg","orcid":"https://orcid.org/0009-0009-0965-2906","observed_orcids":["https://orcid.org/0009-0009-0965-2906"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Peter L\u00f6nnerberg","raw_affiliation_strings":["Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086924433","display_name":"Sten Linnarsson","orcid":"https://orcid.org/0000-0002-3491-3444","observed_orcids":["https://orcid.org/0000-0002-3491-3444"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Sten Linnarsson","raw_affiliation_strings":["Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Biophysics, Laboratory for Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5086924433"],"corresponding_institution_ids":["https://openalex.org/I28166907"],"apc_list":null,"apc_paid":null,"fwci":5.7744,"has_fulltext":false,"cited_by_count":268,"citation_normalized_percentile":{"value":0.97606168,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":100},"biblio":{"volume":"7","issue":"5","first_page":"813","last_page":"828"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-cell-rna-sequencing","display_name":"single-cell RNA sequencing","score":0.925000011920929},{"id":"https://openalex.org/keywords/5-end-sequencing","display_name":"5' end sequencing","score":0.8510000109672546},{"id":"https://openalex.org/keywords/strand-specific-sequencing","display_name":"strand-specific sequencing","score":0.5759999752044678},{"id":"https://openalex.org/keywords/template-switching","display_name":"template switching","score":0.8180000185966492},{"id":"https://openalex.org/keywords/reverse-transcription","display_name":"reverse transcription","score":0.6629999876022339},{"id":"https://openalex.org/keywords/transcription-start-site","display_name":"transcription start sites","score":0.7870000004768372},{"id":"https://openalex.org/keywords/gene-expression-quantification","display_name":"gene expression quantification","score":0.7940000295639038},{"id":"https://openalex.org/keywords/single-cell-transcriptomics","display_name":"single-cell transcriptomics","score":0.4909999966621399}],"concepts":[{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.6303718686103821},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6037799119949341},{"id":"https://openalex.org/C50724178","wikidata":"https://www.wikidata.org/wiki/Q381434","display_name":"Rapid amplification of cDNA ends","level":5,"score":0.5647308230400085},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.560673177242279},{"id":"https://openalex.org/C177284502","wikidata":"https://www.wikidata.org/wiki/Q1005390","display_name":"Adapter (computing)","level":2,"score":0.5368856191635132},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5359194278717041},{"id":"https://openalex.org/C174749747","wikidata":"https://www.wikidata.org/wiki/Q15917269","display_name":"Single cell sequencing","level":5,"score":0.48483502864837646},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.44932278990745544},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.4372747838497162},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.42562809586524963},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4206284284591675},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4010166823863983},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.275886207818985},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.16151553392410278},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.14444595575332642},{"id":"https://openalex.org/C16671776","wikidata":"https://www.wikidata.org/wiki/Q5420592","display_name":"Exome sequencing","level":4,"score":0.08176007866859436},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020121","descriptor_name":"5' Untranslated Regions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1038/nprot.2012.022","doi":"https://doi.org/10.1038/nprot.2012.022","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nprot.2012.022","pdf_url":null,"source":{"id":"https://openalex.org/S109387254","display_name":"Nature Protocols","issn_l":"1750-2799","issn":["1750-2799","1754-2189"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Protocols","raw_type":"journal-article"},{"id":"pmid:22481528","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22481528","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W1969267177","https://openalex.org/W1984984201","https://openalex.org/W1986944049","https://openalex.org/W1990979239","https://openalex.org/W2009458183","https://openalex.org/W2011152694","https://openalex.org/W2015450569","https://openalex.org/W2018246047","https://openalex.org/W2036122200","https://openalex.org/W2041677964","https://openalex.org/W2060245619","https://openalex.org/W2103354991","https://openalex.org/W2117637538","https://openalex.org/W2124985265","https://openalex.org/W2127403997","https://openalex.org/W2127640118","https://openalex.org/W2128227827","https://openalex.org/W2138695384","https://openalex.org/W2141688036","https://openalex.org/W2149573463","https://openalex.org/W2152045787","https://openalex.org/W2152532695","https://openalex.org/W2154431984","https://openalex.org/W2158469604","https://openalex.org/W2171280873","https://openalex.org/W4256135191","https://openalex.org/W7100110722"],"related_works":["https://openalex.org/W1568716789","https://openalex.org/W2576961387","https://openalex.org/W2951404469","https://openalex.org/W3167188751","https://openalex.org/W4226216784","https://openalex.org/W2218420857","https://openalex.org/W7100968397","https://openalex.org/W4399917310","https://openalex.org/W2162554386","https://openalex.org/W4250680253"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":12},{"year":2024,"cited_by_count":16},{"year":2023,"cited_by_count":20},{"year":2022,"cited_by_count":17},{"year":2021,"cited_by_count":23},{"year":2020,"cited_by_count":18},{"year":2019,"cited_by_count":30},{"year":2018,"cited_by_count":15},{"year":2017,"cited_by_count":23},{"year":2016,"cited_by_count":33},{"year":2015,"cited_by_count":21},{"year":2014,"cited_by_count":21},{"year":2013,"cited_by_count":11},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
