{"id":"https://openalex.org/W2015325037","doi":"https://doi.org/10.1071/rdv19n1ab217","title":"217 A NEW APPROACH TO CRYOPRESERVATION OF LARGE EQUINE EMBRYOS BY VITRIFICATION AFTER BLASTOCOEL MICROMANIPULATION","display_name":"217 A NEW APPROACH TO CRYOPRESERVATION OF LARGE EQUINE EMBRYOS BY VITRIFICATION AFTER BLASTOCOEL MICROMANIPULATION","publication_year":2006,"publication_date":"2006-12-12","ids":{"openalex":"https://openalex.org/W2015325037","doi":"https://doi.org/10.1071/rdv19n1ab217","mag":"2015325037"},"language":"en","primary_location":{"id":"doi:10.1071/rdv19n1ab217","doi":"https://doi.org/10.1071/rdv19n1ab217","is_oa":false,"landing_page_url":"https://doi.org/10.1071/rdv19n1ab217","pdf_url":null,"source":{"id":"https://openalex.org/S199941106","display_name":"Reproduction Fertility and Development","issn_l":"1031-3613","issn":["1031-3613","1448-5990"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320302","host_organization_name":"CSIRO Publishing","host_organization_lineage":["https://openalex.org/P4310320302"],"host_organization_lineage_names":["CSIRO Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Reproduction, Fertility and Development","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071901495","display_name":"Jakob Scherzer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J. Scherzer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113453310","display_name":"Richard A. Fayrer-Hosken","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R. A. Fayrer-Hosken","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015870025","display_name":"Lisa J. Ray","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L. Ray","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5109157510","display_name":"G. L. Heusner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G. Heusner","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"19","issue":"1","first_page":"225","last_page":"226"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9829000234603882,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9829000234603882,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.0071000000461936,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cryopreservation","display_name":"cryopreservation","score":0.7850000262260437},{"id":"https://openalex.org/keywords/expanded-blastocysts","display_name":"expanded blastocysts","score":0.703000009059906},{"id":"https://openalex.org/keywords/pregnancy-rate","display_name":"pregnancy rate","score":0.7900000214576721},{"id":"https://openalex.org/keywords/embryo-transfer","display_name":"embryo transfer","score":0.5009999871253967},{"id":"https://openalex.org/keywords/laser-micromanipulation","display_name":"laser micromanipulation","score":0.7089999914169312},{"id":"https://openalex.org/keywords/embryo-size","display_name":"embryo size","score":0.32600000500679016},{"id":"https://openalex.org/keywords/equine-reproduction","display_name":"equine reproduction","score":0.4449999928474426},{"id":"https://openalex.org/keywords/embryo-quality-grading","display_name":"embryo quality grading","score":0.3490000069141388}],"concepts":[{"id":"https://openalex.org/C2778022349","wikidata":"https://www.wikidata.org/wiki/Q1072553","display_name":"Vitrification","level":2,"score":0.9065490365028381},{"id":"https://openalex.org/C179933525","wikidata":"https://www.wikidata.org/wiki/Q1144664","display_name":"Cryopreservation","level":3,"score":0.7310386896133423},{"id":"https://openalex.org/C2778177303","wikidata":"https://www.wikidata.org/wiki/Q227717","display_name":"Blastocyst","level":4,"score":0.7293554544448853},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.7057093381881714},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.6751041412353516},{"id":"https://openalex.org/C2777516009","wikidata":"https://www.wikidata.org/wiki/Q194207","display_name":"Ethylene glycol","level":2,"score":0.6615932583808899},{"id":"https://openalex.org/C2780881558","wikidata":"https://www.wikidata.org/wiki/Q132501","display_name":"Glycerol","level":2,"score":0.5834606289863586},{"id":"https://openalex.org/C14814345","wikidata":"https://www.wikidata.org/wiki/Q589646","display_name":"Theriogenology","level":5,"score":0.4474742114543915},{"id":"https://openalex.org/C59843425","wikidata":"https://www.wikidata.org/wiki/Q5370216","display_name":"Embryo cryopreservation","level":4,"score":0.43494805693626404},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41408926248550415},{"id":"https://openalex.org/C159879943","wikidata":"https://www.wikidata.org/wiki/Q14890574","display_name":"Fertilisation","level":5,"score":0.41124042868614197},{"id":"https://openalex.org/C2994278967","wikidata":"https://www.wikidata.org/wiki/Q7314318","display_name":"Reproductive technology","level":4,"score":0.39337682723999023},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34898024797439575},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.33693927526474},{"id":"https://openalex.org/C29456083","wikidata":"https://www.wikidata.org/wiki/Q1221899","display_name":"Gynecology","level":1,"score":0.32590198516845703},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1983841061592102},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.06665706634521484},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.05207076668739319},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1071/rdv19n1ab217","doi":"https://doi.org/10.1071/rdv19n1ab217","is_oa":false,"landing_page_url":"https://doi.org/10.1071/rdv19n1ab217","pdf_url":null,"source":{"id":"https://openalex.org/S199941106","display_name":"Reproduction Fertility and Development","issn_l":"1031-3613","issn":["1031-3613","1448-5990"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320302","host_organization_name":"CSIRO Publishing","host_organization_lineage":["https://openalex.org/P4310320302"],"host_organization_lineage_names":["CSIRO Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Reproduction, Fertility and Development","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2088737747","https://openalex.org/W4210503108","https://openalex.org/W2128261677","https://openalex.org/W2901914599","https://openalex.org/W3049765352","https://openalex.org/W3112519783","https://openalex.org/W2089664310","https://openalex.org/W1970180760","https://openalex.org/W2024810792","https://openalex.org/W2232806886"],"abstract_inverted_index":{"Vitrified":[0],"large":[1,496],"equine":[2,497],"embryos":[3,73,108,126,178,195,279,320,363,413,498],">800":[4],"\u00b5m":[5,81,85,88,91,95],"recovered":[6,46],"on":[7,39,325,460,499],"Day":[8,326,452,461],"8":[9,327],"after":[10,130,291,328,386,400,454],"ovulation":[11],"have":[12],"not":[13,67],"been":[14],"successfully":[15],"transferred":[16,53,372],"yet.":[17],"In":[18,42,193],"this":[19,76,490],"study,":[20],"we":[21,487],"examined":[22,406],"the":[23,28,48,134,163,286,383,411,415,419,424,431,463,467,477,493,504],"effects":[24],"of":[25,27,33,60,72,119,133,162,247,277,377,402,410,426,438,443,476,495],"reduction":[26],"blastocoelic":[29,135,165,439],"fluid":[30,136,166,440],"and":[31,93,128,137,146,173,200,215,284,289,308,315,340,357,370,389,441],"microinfusion":[32,118,139,442],"a":[34,148,170,259,354,457,500],"cryopreservative":[35],"prior":[36,282,293],"to":[37,104,111,198,283,294,306,323,373,423],"vitrification":[38,77,287],"pregnancy":[40,58],"outcome.":[41],"2006,":[43],"33":[44],"embryos,":[45,403],"at":[47,334,344,451],"expanded":[49,298],"blastocyst":[50],"stage,":[51],"were":[52,79,98,109,179,196,233,253,280,321,351,364,405],"fresh":[54],"with":[55],"an":[56,367,427,448],"average":[57],"rate":[59],"91%":[61],"(30/33).":[62],"However,":[63,430],"suitable":[64],"recipients":[65,324,404],"are":[66],"always":[68],"available.":[69],"The":[70,267,375],"sizes":[71],"used":[74],"for":[75,140,212,227,255,337,346,492],"project":[78],"805":[80],"(embryo":[82],"#1),":[83],"820":[84],"(#2),":[86],"1120":[87],"(#3),":[89],"1286":[90],"(#4),":[92],"979":[94],"(#5).":[96],"They":[97],"all":[99,278],"morphologically":[100],"graded":[101],"excellent":[102],"(according":[103],"IETS":[105],"guidelines).":[106],"These":[107],"assigned":[110],"either":[112],"no":[113],"micromanipulation":[114],"(embryos":[115],"#1\u2013#3)":[116],"or":[117],"VS1":[120,199,444],"(1.4":[121,202],"M":[122,203,207,218,222,249],"glycerol":[123,204,219],"in":[124,210,225,231,302,332,342,418,466],"PBS;":[125],"#4":[127],"#5)":[129],"additional":[131],"aspiration":[132,145,437],"before":[138],"embryo":[141,295,380,391,417],"#5.":[142],"To":[143],"facilitate":[144],"microinfusion,":[147],"laser":[149],"system":[150,175],"(XYclone;":[151],"Hamilton":[152],"Thorne":[153],"Biosciences,":[154],"Beverly,":[155],"MA,":[156],"USA)":[157],"was":[158,167,269,392,469,480],"applied.":[159],"Approximately":[160],"20%":[161],"total":[164],"removed":[168],"using":[169],"standard":[171],"micromanipulator":[172],"microcapillary":[174],"(Eppendorf).":[176],"All":[177,297],"then":[180,234,341],"vitrified":[181,319],"as":[182],"previously":[183],"described":[184],"(Eldridge-Panuska":[185],"et":[186],"al":[187],".":[188],"2005":[189],"Theriogenology":[190],"63,":[191],"1308\u20131319).":[192],"brief,":[194],"exposed":[197],"VS2":[201,307],"+":[205,220],"3.6":[206],"ethylene":[208,223],"glycol":[209,224],"PBS)":[211,226],"5":[213,338,360,384],"min,":[214,361],"VS3":[216,232],"(3.4":[217],"6.6":[221],"1":[228,256],"min.":[229],"Embryos":[230],"individually":[235],"loaded":[236,365],"into":[237,258,272,353,366,394],"0.25-mL":[238],"straws,":[239],"separated":[240],"by":[241,264,407,483],"2":[242],"air":[243,333],"bubbles":[244],"from":[245],"columns":[246],"0.5":[248],"galactose.":[250],"Then":[251],"straws":[252],"placed":[254],"min":[257,385],"cooled":[260],"plastic":[261],"goblet":[262,268],"surrounded":[263],"liquid":[265,273],"nitrogen.":[266,274],"finally":[270],"plunged":[271],"Digital":[275],"images":[276],"taken":[281],"during":[285,382,503],"procedure,":[288],"also":[290],"thawing":[292,331,387],"transfer.":[296],"blastocysts":[299],"initially":[300],"decreased":[301],"size.":[303],"After":[304,330,359],"transfer":[305,401],"VS3,":[309],"they":[310],"lost":[311],"their":[312],"spherical":[313],"shape":[314],"blastocoels":[316],"collapsed.":[317],"Four":[318],"transfered":[322],"ovulation.":[329,455],"room":[335],"temperature":[336],"s":[339],"water":[343],"30\u00b0C":[345],"15":[347,453],"s,":[348],"straw":[349],"contents":[350],"emptied":[352],"Petri":[355],"dish":[356],"mixed.":[358],"single":[362],"AI":[368],"pipette":[369],"nonsurgically":[371],"recipients.":[374],"blastocoel":[376],"only":[378],"one":[379,390],"re-expanded":[381],"(#3)":[388],"split":[393,416],"two":[395],"halves":[396],"(#4).":[397],"One":[398],"week":[399],"ultrasonography.":[408],"None":[409],"control":[412],"nor":[414],"treatment":[420],"group":[421],"led":[422],"formation":[425],"embryonic":[428,449],"vesicle.":[429],"blastocyst,":[432],"which":[433,479],"had":[434,446],"undergone":[435],"both":[436],"(#5),":[445],"formed":[447],"vesicle":[450],"During":[456],"further":[458],"exam":[459],"28,":[462],"uterine":[464],"tone":[465],"recipient":[468],"still":[470],"increased,":[471],"but":[472],"ultrasonography":[473],"revealed":[474],"resorption":[475],"embryo,":[478],"probably":[481],"caused":[482],"heat":[484],"stress.":[485],"Nevertheless,":[486],"will":[488],"test":[489],"protocol":[491],"cryopreservation":[494],"larger":[501],"scale":[502],"next":[505],"breeding":[506],"season.":[507]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}