{"id":"https://openalex.org/W2015314915","doi":"https://doi.org/10.1021/bi00253a022","title":"Identifying Substrate Motifs of Protein Kinases by a Random Library Approach","display_name":"Identifying Substrate Motifs of Protein Kinases by a Random Library Approach","publication_year":1994,"publication_date":"1994-12-01","ids":{"openalex":"https://openalex.org/W2015314915","doi":"https://doi.org/10.1021/bi00253a022","mag":"2015314915","pmid":"https://pubmed.ncbi.nlm.nih.gov/7993909"},"language":"en","primary_location":{"id":"doi:10.1021/bi00253a022","doi":"https://doi.org/10.1021/bi00253a022","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00253a022","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038683160","display_name":"Jinzi J. Wu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jinzi Wu","raw_affiliation_strings":["Selectide Corporation, Tucson, Arizona 85737"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Selectide Corporation, Tucson, Arizona 85737","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5107703002","display_name":"N. Qingyan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Qingyan N. Ma","raw_affiliation_strings":["Selectide Corporation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Selectide Corporation","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016566536","display_name":"Kit S. Lam","orcid":"https://orcid.org/0000-0002-3076-6969","observed_orcids":["https://orcid.org/0000-0002-3076-6969"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kit S. Lam","raw_affiliation_strings":["University of Arizona"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Arizona","institution_ids":["https://openalex.org/I138006243"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.7607,"has_fulltext":false,"cited_by_count":104,"citation_normalized_percentile":{"value":0.91642097,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"33","issue":"49","first_page":"14825","last_page":"14833"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.5557000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.5557000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.40700000524520874,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.01759999990463257,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-phosphorylation","display_name":"protein phosphorylation","score":0.7519999742507935},{"id":"https://openalex.org/keywords/protein-kinase-a","display_name":"protein kinase A","score":0.9369999766349792},{"id":"https://openalex.org/keywords/combinatorial-peptide-libraries","display_name":"combinatorial peptide libraries","score":0.921999990940094},{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.781000018119812},{"id":"https://openalex.org/keywords/signal-transduction","display_name":"signal transduction","score":0.8019999861717224}],"concepts":[{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.7531986832618713},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6296031475067139},{"id":"https://openalex.org/C2778447006","wikidata":"https://www.wikidata.org/wiki/Q1156474","display_name":"Icon","level":2,"score":0.6168420314788818},{"id":"https://openalex.org/C2778407487","wikidata":"https://www.wikidata.org/wiki/Q14565201","display_name":"Altmetrics","level":2,"score":0.6036607623100281},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.5236327648162842},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.47433704137802124},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.40254899859428406},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011499","descriptor_name":"Protein Processing, Post-Translational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017868","descriptor_name":"Cyclic AMP-Dependent Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00253a022","doi":"https://doi.org/10.1021/bi00253a022","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00253a022","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:7993909","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7993909","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2159449340","display_name":null,"funder_award_id":"CA 23074","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G3494388860","display_name":"Therapeutic Targeting of Hypoxic and Oxidative Stress","funder_award_id":"P01CA017094","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G4205871284","display_name":"DISCOVERY OF PEPTIDE ANTICANCER DRUGS","funder_award_id":"U01CA057723","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G563043198","display_name":null,"funder_award_id":"CA-170-94","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6002107373","display_name":null,"funder_award_id":"CA57723","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000909272","display_name":"University of Arizona Cancer Center - Cancer Center Support Grant","funder_award_id":"P30CA023074","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W32049051","https://openalex.org/W179113440","https://openalex.org/W222506362","https://openalex.org/W346745165","https://openalex.org/W650240373","https://openalex.org/W1011007954","https://openalex.org/W1490238718","https://openalex.org/W1493020321","https://openalex.org/W1507957953","https://openalex.org/W1543158853","https://openalex.org/W1555106203","https://openalex.org/W1566146550","https://openalex.org/W1571007526","https://openalex.org/W1573821584","https://openalex.org/W1579416891","https://openalex.org/W1589074645","https://openalex.org/W1850214287","https://openalex.org/W1965867424","https://openalex.org/W1971475788","https://openalex.org/W1979111516","https://openalex.org/W1999157780","https://openalex.org/W2004686233","https://openalex.org/W2004917770","https://openalex.org/W2006446936","https://openalex.org/W2010808792","https://openalex.org/W2017038541","https://openalex.org/W2019744607","https://openalex.org/W2020257412","https://openalex.org/W2024308660","https://openalex.org/W2041719513","https://openalex.org/W2045876058","https://openalex.org/W2046360185","https://openalex.org/W2058358294","https://openalex.org/W2065236789","https://openalex.org/W2070023258","https://openalex.org/W2079896268","https://openalex.org/W2080262597","https://openalex.org/W2095296343","https://openalex.org/W2095423611","https://openalex.org/W2100238612","https://openalex.org/W2103879677","https://openalex.org/W2127728110","https://openalex.org/W2164682544","https://openalex.org/W2195964294","https://openalex.org/W2222709349"],"related_works":["https://openalex.org/W2401006823","https://openalex.org/W2412625071","https://openalex.org/W2010161321","https://openalex.org/W1850964633","https://openalex.org/W3169757395","https://openalex.org/W831481183","https://openalex.org/W2072009508","https://openalex.org/W2090650753","https://openalex.org/W2106983325","https://openalex.org/W1999450178"],"abstract_inverted_index":{"Protein":[0],"phosphorylation":[1],"is":[2,68,113,147,184],"an":[3],"important":[4],"posttranslational":[5,219],"modification":[6],"process":[7],"that":[8,150,163,181],"plays":[9],"a":[10,55,104,114,209],"crucial":[11],"role":[12],"in":[13,22,32,107,152],"signal":[14,33],"transduction.":[15],"There":[16],"are":[17,35,44,165],"many":[18,29],"protein":[19,30,64,100,193],"kinases":[20,31,194],"involved":[21],"cell":[23],"signaling.":[24],"However,":[25],"substrate":[26,61,189,215],"motifs":[27,43,62,190,216],"of":[28,63,73,127,157,191],"transduction":[34],"not":[36],"well-known.":[37],"Traditional":[38],"methodologies":[39],"for":[40,58,136,142,168,187,212,217],"identifying":[41,188,213],"these":[42],"often":[45],"difficult":[46],"and":[47,119,129,146,198],"inefficient.":[48],"In":[49,200],"the":[50,71,108,125,153,158],"present":[51,109],"study,":[52],"we":[53],"developed":[54],"novel":[56],"approach":[57],"discovering":[59],"linear":[60,214],"kinases.":[65],"This":[66],"method":[67,183,203,211],"based":[69],"on":[70,79],"screening":[72],"random":[74,172],"synthetic":[75,173],"combinatorial":[76,174],"peptide":[77,87,140,175],"libraries":[78,121],"beads":[80],"where":[81],"each":[82],"bead":[83],"expresses":[84],"only":[85],"one":[86],"entity":[88],"[Lam":[89],"et":[90],"al.":[91],"(1991)":[92],"Nature":[93],"354,":[94],"82-84].":[95],"We":[96],"chose":[97],"cyclic":[98],"AMP-dependent":[99],"kinase":[101],"(cAPK)":[102],"as":[103,208],"model":[105],"system":[106],"study":[110],"since":[111],"it":[112],"well-studied":[115],"enzyme.":[116],"Random":[117],"pentapeptide":[118],"heptapeptide":[120],"were":[122,133],"screened":[123],"with":[124,195],"addition":[126],"[gamma-32P]ATP":[128],"cAPK.":[130],"32P-Labeled":[131],"peptide-beads":[132],"then":[134],"isolated":[135],"microsequencing.":[137],"The":[138],"identified":[139],"motif":[141],"cAPK":[143,169],"was":[144],"RRXS":[145],"identical":[148],"to":[149],"reported":[151],"literature.":[154],"Kinetic":[155],"studies":[156],"best":[159],"three":[160],"peptides":[161],"indicate":[162],"they":[164],"efficient":[166],"substrates":[167],"discovered":[170],"from":[171],"libraries.":[176],"Our":[177],"results":[178],"also":[179,205],"suggest":[180],"this":[182,202],"potentially":[185],"useful":[186],"various":[192,218],"high":[196],"sensitivity":[197],"specificity.":[199],"addition,":[201],"can":[204],"be":[206],"used":[207],"general":[210],"modifications.":[220]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}