{"id":"https://openalex.org/W2015280724","doi":"https://doi.org/10.1021/cb5006596","title":"Recoding Aminoacyl-tRNA Synthetases for Synthetic Biology by Rational Protein-RNA Engineering","display_name":"Recoding Aminoacyl-tRNA Synthetases for Synthetic Biology by Rational Protein-RNA Engineering","publication_year":2014,"publication_date":"2014-10-13","ids":{"openalex":"https://openalex.org/W2015280724","doi":"https://doi.org/10.1021/cb5006596","mag":"2015280724","pmid":"https://pubmed.ncbi.nlm.nih.gov/25310879","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4273986"},"language":"en","primary_location":{"id":"doi:10.1021/cb5006596","doi":"https://doi.org/10.1021/cb5006596","is_oa":true,"landing_page_url":"https://doi.org/10.1021/cb5006596","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/cb5006596?ref=article_openPDF","source":{"id":"https://openalex.org/S118914585","display_name":"ACS Chemical Biology","issn_l":"1554-8929","issn":["1554-8929","1554-8937"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Chemical Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://pubs.acs.org/doi/pdf/10.1021/cb5006596?ref=article_openPDF","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023336794","display_name":"Andrew G. Hadd","orcid":"https://orcid.org/0009-0000-6274-0555","observed_orcids":["https://orcid.org/0009-0000-6274-0555"]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew Hadd","raw_affiliation_strings":["Oregon Health & Sciences University , , 3181 Southwest Sam Jackson Park Road , , ,","Department of Biochemistry & Molecular Biology, Oregon Health & Sciences University , 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Oregon Health & Sciences University , , 3181 Southwest Sam Jackson Park Road , , ,","institution_ids":["https://openalex.org/I165690674"]},{"raw_affiliation_string":"Department of Biochemistry & Molecular Biology, Oregon Health & Sciences University , 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239, United States","institution_ids":["https://openalex.org/I165690674"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056156015","display_name":"John J. Perona","orcid":"https://orcid.org/0000-0002-2219-3021","observed_orcids":["https://orcid.org/0000-0002-2219-3021"]},"institutions":[{"id":"https://openalex.org/I126345244","display_name":"Portland State University","ror":"https://ror.org/00yn2fy02","country_code":"US","type":"education","lineage":["https://openalex.org/I126345244"]},{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John J. Perona","raw_affiliation_strings":["Oregon Health & Sciences University , , 3181 Southwest Sam Jackson Park Road , , ,","Portland State University , , PO Box 751 , , ,","Department of Biochemistry & Molecular Biology, Oregon Health & Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239, United States","Department#R#of Chemistry, Portland State University, PO Box 751, Portland, Oregon 97207, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Oregon Health & Sciences University , , 3181 Southwest Sam Jackson Park Road , , ,","institution_ids":["https://openalex.org/I165690674"]},{"raw_affiliation_string":"Portland State University , , PO Box 751 , , ,","institution_ids":["https://openalex.org/I126345244"]},{"raw_affiliation_string":"Department of Biochemistry & Molecular Biology, Oregon Health & Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239, United States","institution_ids":["https://openalex.org/I165690674"]},{"raw_affiliation_string":"Department#R#of Chemistry, Portland State University, PO Box 751, Portland, Oregon 97207, United States","institution_ids":["https://openalex.org/I126345244"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5056156015"],"corresponding_institution_ids":["https://openalex.org/I126345244","https://openalex.org/I165690674"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":{"value":4500,"currency":"USD","value_usd":4500},"fwci":0.5464,"has_fulltext":true,"cited_by_count":12,"citation_normalized_percentile":{"value":0.6326837,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"9","issue":"12","first_page":"2761","last_page":"2766"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aminoacyl-trna-synthetase","display_name":"aminoacyl-tRNA synthetase","score":0.9810000061988831},{"id":"https://openalex.org/keywords/glutaminyl-trna-synthetase","display_name":"glutaminyl-tRNA synthetase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/trna-gln","display_name":"tRNA-Gln","score":0.5460000038146973},{"id":"https://openalex.org/keywords/unnatural-amino-acids","display_name":"unnatural amino acids","score":0.824999988079071},{"id":"https://openalex.org/keywords/synthetic-biology","display_name":"synthetic biology","score":0.6589999794960022}],"concepts":[{"id":"https://openalex.org/C53105671","wikidata":"https://www.wikidata.org/wiki/Q14777816","display_name":"Rational design","level":2,"score":0.821863055229187},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.7482208013534546},{"id":"https://openalex.org/C2779315393","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Aminoacyl tRNA synthetase","level":5,"score":0.6941404938697815},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.693874180316925},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.6158750653266907},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6000998020172119},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.5992366671562195},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.5472356081008911},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5374195575714111},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.5007317066192627},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4963248372077942},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4841328561306},{"id":"https://openalex.org/C152769699","wikidata":"https://www.wikidata.org/wiki/Q410814","display_name":"Protein design","level":3,"score":0.45758500695228577},{"id":"https://openalex.org/C2910418148","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Amino Acyl-tRNA Synthetases","level":5,"score":0.4189794361591339},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3623524308204651},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.33573126792907715},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.22858858108520508},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.12470123171806335},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12355366349220276}],"mesh":[{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012346","descriptor_name":"RNA, Transfer, Amino Acyl","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012346","descriptor_name":"RNA, Transfer, Amino Acyl","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1021/cb5006596","doi":"https://doi.org/10.1021/cb5006596","is_oa":true,"landing_page_url":"https://doi.org/10.1021/cb5006596","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/cb5006596?ref=article_openPDF","source":{"id":"https://openalex.org/S118914585","display_name":"ACS Chemical Biology","issn_l":"1554-8929","issn":["1554-8929","1554-8937"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Chemical Biology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3576717","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4273986","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4273986","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4273986","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Chem Biol","raw_type":"Text"},{"id":"pmid:25310879","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25310879","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS chemical biology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.813.2769","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.813.2769","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/e1/73/cb5006596.PMC4273986.pdf","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1021/cb5006596","doi":"https://doi.org/10.1021/cb5006596","is_oa":true,"landing_page_url":"https://doi.org/10.1021/cb5006596","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/cb5006596?ref=article_openPDF","source":{"id":"https://openalex.org/S118914585","display_name":"ACS Chemical Biology","issn_l":"1554-8929","issn":["1554-8929","1554-8937"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Chemical Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3854385263","display_name":null,"funder_award_id":"GM 63713","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G3861822500","display_name":"Specificity in the Synthesis of Aminoacyl-tRNA","funder_award_id":"R01GM063713","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320313720","display_name":"National Collegiate Athletic Association","ror":"https://ror.org/014cfdh55"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2015280724.pdf","grobid_xml":"https://content.openalex.org/works/W2015280724.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W1797223236","https://openalex.org/W2001373414","https://openalex.org/W2002336386","https://openalex.org/W2004235182","https://openalex.org/W2018756788","https://openalex.org/W2036130241","https://openalex.org/W2042432823","https://openalex.org/W2043249509","https://openalex.org/W2046270994","https://openalex.org/W2051556659","https://openalex.org/W2052858356","https://openalex.org/W2057892106","https://openalex.org/W2058487848","https://openalex.org/W2060391020","https://openalex.org/W2061541382","https://openalex.org/W2062660287","https://openalex.org/W2062819748","https://openalex.org/W2071213340","https://openalex.org/W2074244626","https://openalex.org/W2077261712","https://openalex.org/W2077855194","https://openalex.org/W2079435580","https://openalex.org/W2083822402","https://openalex.org/W2085791091","https://openalex.org/W2092118306","https://openalex.org/W2095554932","https://openalex.org/W2103563524","https://openalex.org/W2115457325","https://openalex.org/W2126855067","https://openalex.org/W2148123458","https://openalex.org/W2148313902","https://openalex.org/W2156377423","https://openalex.org/W2157680389","https://openalex.org/W2161997993","https://openalex.org/W2172121653","https://openalex.org/W2316970422"],"related_works":["https://openalex.org/W2079435580","https://openalex.org/W2071213340","https://openalex.org/W2036078885","https://openalex.org/W7100478801","https://openalex.org/W2156377423","https://openalex.org/W2161997993","https://openalex.org/W2316970422","https://openalex.org/W3084387642","https://openalex.org/W1997357285","https://openalex.org/W2084639487"],"abstract_inverted_index":{"We":[0],"have":[1],"taken":[2],"a":[3,64],"rational":[4,91],"approach":[5],"to":[6,41,96],"redesigning":[7],"the":[8,29,42,49,56,61,72,75,113],"amino":[9,78,104],"acid":[10],"binding":[11],"and":[12,27,92],"aminoacyl-tRNA":[13,99],"pairing":[14,30],"specificities":[15],"of":[16,32,48,55,60,74,112],"bacterial":[17],"glutaminyl-tRNA":[18],"synthetase.":[19],"The":[20],"four-stage":[21],"engineering":[22,84],"incorporates":[23],"generalizable":[24],"design":[25,97],"principles":[26],"improves":[28],"efficiency":[31],"noncognate":[33],"glutamate":[34],"with":[35],"tRNA(Gln)":[36],"by":[37],"over":[38],"10(5)-fold":[39],"compared":[40],"wild-type":[43],"enzyme.":[44],"Better":[45],"optimized":[46],"designs":[47],"protein-RNA":[50],"complex":[51],"include":[52],"substantial":[53],"reengineering":[54],"globular":[57],"core":[58],"region":[59],"tRNA,":[62],"demonstrating":[63],"role":[65],"for":[66,89,117],"specific":[67],"tRNA":[68],"nucleotides":[69],"in":[70,119],"specifying":[71],"identity":[73],"genetically":[76],"encoded":[77],"acid.":[79],"Principles":[80],"emerging":[81],"from":[82],"this":[83],"effort":[85],"open":[86],"new":[87],"prospects":[88],"combining":[90],"genetic":[93],"selection":[94],"approaches":[95],"novel":[98],"synthetases":[100],"that":[101],"ligate":[102],"noncanonical":[103],"acids":[105],"onto":[106],"tRNAs.":[107],"This":[108],"will":[109],"facilitate":[110],"reconstruction":[111],"cellular":[114],"translation":[115],"apparatus":[116],"applications":[118],"synthetic":[120],"biology.":[121]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}