{"id":"https://openalex.org/W2015148235","doi":"https://doi.org/10.1210/me.15.3.458","title":"Heterodimerization between the Glucocorticoid Receptor and the Unrelated DNA-Binding Protein, Xenopus Glucocorticoid Receptor Accessory Factor","display_name":"Heterodimerization between the Glucocorticoid Receptor and the Unrelated DNA-Binding Protein, Xenopus Glucocorticoid Receptor Accessory Factor","publication_year":2001,"publication_date":"2001-03-01","ids":{"openalex":"https://openalex.org/W2015148235","doi":"https://doi.org/10.1210/me.15.3.458","mag":"2015148235","pmid":"https://pubmed.ncbi.nlm.nih.gov/11222746"},"language":"en","primary_location":{"id":"doi:10.1210/me.15.3.458","doi":"https://doi.org/10.1210/me.15.3.458","is_oa":true,"landing_page_url":"https://doi.org/10.1210/me.15.3.458","pdf_url":"https://academic.oup.com/mend/article-pdf/15/3/458/8950758/mend0458.pdf","source":{"id":"https://openalex.org/S57449756","display_name":"Molecular Endocrinology","issn_l":"0888-8809","issn":["0888-8809","1944-9917"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Endocrinology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/mend/article-pdf/15/3/458/8950758/mend0458.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085355773","display_name":"Brian Morin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian Morin","raw_affiliation_strings":["Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212, USA","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082707932","display_name":"Glenna R. Woodcock","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Glenna R. Woodcock","raw_affiliation_strings":["Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089092205","display_name":"LaNita A. Nichols","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"LaNita A. Nichols","raw_affiliation_strings":["Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111713135","display_name":"Len\u00e9 J. Holland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Len\u00e9 J. Holland","raw_affiliation_strings":["Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology University of Missouri-Columbia School of Medicine Columbia, Missouri 65212","institution_ids":["https://openalex.org/I76835614"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5111713135"],"corresponding_institution_ids":["https://openalex.org/I76835614"],"apc_list":null,"apc_paid":null,"fwci":0.364,"has_fulltext":true,"cited_by_count":8,"citation_normalized_percentile":{"value":0.60847053,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"15","issue":"3","first_page":"458","last_page":"466"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10756","display_name":"Estrogen and related hormone effects","score":0.9692000150680542,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10756","display_name":"Estrogen and related hormone effects","score":0.9692000150680542,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.018799999728798866,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12421","display_name":"Adrenal Hormones and Disorders","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glucocorticoid-receptor","display_name":"glucocorticoid receptor","score":0.9919999837875366},{"id":"https://openalex.org/keywords/heterodimerization","display_name":"heterodimerization","score":0.8519999980926514},{"id":"https://openalex.org/keywords/glucocorticoid-response-element","display_name":"glucocorticoid response element","score":0.8389999866485596},{"id":"https://openalex.org/keywords/transcriptional-regulation","display_name":"transcriptional regulation","score":0.7730000019073486},{"id":"https://openalex.org/keywords/cooperative-dna-binding","display_name":"cooperative DNA binding","score":0.7269999980926514},{"id":"https://openalex.org/keywords/xenopus-laevis","display_name":"Xenopus laevis","score":0.7250000238418579},{"id":"https://openalex.org/keywords/hepatocytes","display_name":"hepatocytes","score":0.5389999747276306}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8206660747528076},{"id":"https://openalex.org/C59493245","wikidata":"https://www.wikidata.org/wiki/Q14877410","display_name":"Glucocorticoid receptor","level":3,"score":0.8013033866882324},{"id":"https://openalex.org/C51445715","wikidata":"https://www.wikidata.org/wiki/Q410477","display_name":"Hormone response element","level":5,"score":0.7226207256317139},{"id":"https://openalex.org/C2779535977","wikidata":"https://www.wikidata.org/wiki/Q1342298","display_name":"Xenopus","level":3,"score":0.6382647156715393},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5777643322944641},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.5152021050453186},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5030471682548523},{"id":"https://openalex.org/C2780841215","wikidata":"https://www.wikidata.org/wiki/Q422549","display_name":"Glucocorticoid","level":2,"score":0.4581649899482727},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.37338346242904663},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3515593111515045},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.27678292989730835},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22656330466270447},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.20342692732810974},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.07743090391159058},{"id":"https://openalex.org/C84606932","wikidata":"https://www.wikidata.org/wiki/Q416496","display_name":"Estrogen receptor","level":4,"score":0.0},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.0},{"id":"https://openalex.org/C530470458","wikidata":"https://www.wikidata.org/wiki/Q128581","display_name":"Breast cancer","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001667","descriptor_name":"Binding, Competitive","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005340","descriptor_name":"Fibrinogen","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009687","descriptor_name":"Nuclear Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009687","descriptor_name":"Nuclear Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011965","descriptor_name":"Receptors, Glucocorticoid","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011965","descriptor_name":"Receptors, Glucocorticoid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014982","descriptor_name":"Xenopus laevis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019281","descriptor_name":"Dimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029867","descriptor_name":"Xenopus Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1210/me.15.3.458","doi":"https://doi.org/10.1210/me.15.3.458","is_oa":true,"landing_page_url":"https://doi.org/10.1210/me.15.3.458","pdf_url":"https://academic.oup.com/mend/article-pdf/15/3/458/8950758/mend0458.pdf","source":{"id":"https://openalex.org/S57449756","display_name":"Molecular Endocrinology","issn_l":"0888-8809","issn":["0888-8809","1944-9917"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Endocrinology","raw_type":"journal-article"},{"id":"doi:10.1210/mend.15.3.0607","doi":"https://doi.org/10.1210/mend.15.3.0607","is_oa":false,"landing_page_url":"https://doi.org/10.1210/mend.15.3.0607","pdf_url":null,"source":{"id":"https://openalex.org/S57449756","display_name":"Molecular Endocrinology","issn_l":"0888-8809","issn":["0888-8809","1944-9917"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Endocrinology","raw_type":"journal-article"},{"id":"pmid:11222746","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11222746","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular endocrinology (Baltimore, Md.)","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1210/me.15.3.458","doi":"https://doi.org/10.1210/me.15.3.458","is_oa":true,"landing_page_url":"https://doi.org/10.1210/me.15.3.458","pdf_url":"https://academic.oup.com/mend/article-pdf/15/3/458/8950758/mend0458.pdf","source":{"id":"https://openalex.org/S57449756","display_name":"Molecular Endocrinology","issn_l":"0888-8809","issn":["0888-8809","1944-9917"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Endocrinology","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306230","display_name":"American Heart Association","ror":"https://ror.org/013kjyp64"},{"id":"https://openalex.org/F4320309568","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2015148235.pdf","grobid_xml":"https://content.openalex.org/works/W2015148235.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W1509565492","https://openalex.org/W1513717105","https://openalex.org/W1551136566","https://openalex.org/W1554294376","https://openalex.org/W1775297890","https://openalex.org/W1968905365","https://openalex.org/W1986368022","https://openalex.org/W1989041945","https://openalex.org/W1997510672","https://openalex.org/W2000400976","https://openalex.org/W2003196551","https://openalex.org/W2018499187","https://openalex.org/W2030110709","https://openalex.org/W2035415315","https://openalex.org/W2041781038","https://openalex.org/W2048327622","https://openalex.org/W2048670847","https://openalex.org/W2058081875","https://openalex.org/W2071263028","https://openalex.org/W2071525868","https://openalex.org/W2078001389","https://openalex.org/W2084885861","https://openalex.org/W2095437984","https://openalex.org/W2095465583","https://openalex.org/W2097237892","https://openalex.org/W2119725374","https://openalex.org/W2128840922","https://openalex.org/W2132251976","https://openalex.org/W2144634347","https://openalex.org/W2158022708","https://openalex.org/W2172256092","https://openalex.org/W2180559065","https://openalex.org/W2207136697","https://openalex.org/W2605614532","https://openalex.org/W2764384160","https://openalex.org/W4248190808"],"related_works":["https://openalex.org/W2018499187","https://openalex.org/W1989041945","https://openalex.org/W2078001389","https://openalex.org/W2035490496","https://openalex.org/W1993482635","https://openalex.org/W194299743","https://openalex.org/W1968154721","https://openalex.org/W2611421680","https://openalex.org/W2048118699","https://openalex.org/W2085844886"],"abstract_inverted_index":{"The":[0,82,141,191],"adrenal":[1],"steroid":[2],"hormones,":[3],"glucocorticoids,":[4],"control":[5],"many":[6],"physiological":[7],"responses":[8],"to":[9,120,130],"trauma,":[10],"including":[11],"elevated":[12],"synthesis":[13],"of":[14,22,84,94,101,116,139,171,179,182,202,206,216],"fibrinogen,":[15],"a":[16,33,44,59,78,137,203,213,223,227],"major":[17],"blood-clotting":[18],"protein.":[19],"Glucocorticoid":[20],"regulation":[21,218],"the":[23,54,66,71,95,99,102,121,162],"gamma-fibrinogen":[24],"subunit":[25],"gene":[26],"in":[27,77,174,185,219],"Xenopus":[28,37,189],"laevis":[29],"is":[30,86,154,159,200],"mediated":[31],"by":[32,58,75,88,111,136],"binding":[34,115,129,164],"site":[35,165],"for":[36,194],"glucocorticoid":[38,46],"receptor":[39],"accessory":[40],"factor":[41,138],"(XGRAF)":[42],"and":[43,65,105,118,146,166,198,222],"contiguous":[45],"response":[47],"element":[48],"(GRE)":[49],"half-site.":[50],"Here,":[51],"we":[52],"characterize":[53],"protein:DNA":[55],"complex":[56,72,97,109],"formed":[57],"cooperative":[60,142],"interaction":[61,143],"between":[62,144,161,196],"XGRAF,":[63],"GR,":[64],"DNA.":[67],"We":[68],"demonstrate":[69],"that":[70,126],"contains":[73],"XGRAF":[74,117,145,163,180,197],"competition":[76],"gel":[79],"shift":[80],"assay.":[81],"presence":[83],"GR":[85,103,112,119,127,147,183,199,221],"established":[87],"two":[89,208],"criteria:":[90],"1)":[91],"size":[92,100],"dependence":[93],"XGRAF:GR:DNA":[96],"on":[98,150],"component":[104],"2)":[106],"interference":[107],"with":[108,133,177,229],"formation":[110,201],"antibody.":[113],"Cooperative":[114],"DNA":[122,135,152,235],"was":[123],"quantitated,":[124],"showing":[125],"favors":[128],"XGRAF:DNA":[131],"compared":[132],"free":[134],"30.":[140],"can":[148],"occur":[149],"nicked":[151],"but":[153],"disrupted":[155],"when":[156],"1":[157],"bp":[158],"inserted":[160],"half-GRE.":[167],"Significantly,":[168],"this":[169],"loss":[170,178],"physical":[172],"association":[173],"vitro":[175],"correlates":[176],"amplification":[181],"activity":[184],"transiently":[186],"transfected":[187],"primary":[188],"hepatocytes.":[190],"simplest":[192],"explanation":[193],"cooperativity":[195],"DNA-bound":[204],"heterodimer":[205],"these":[207],"proteins.":[209],"This":[210],"mechanism":[211],"represents":[212],"new":[214],"mode":[215],"transcriptional":[217],"which":[220],"nonreceptor":[224],"protein":[225],"form":[226],"heterodimer,":[228],"both":[230],"partners":[231],"contacting":[232],"their":[233],"specific":[234],"sites":[236],"simultaneously.":[237]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}