{"id":"https://openalex.org/W2015120149","doi":"https://doi.org/10.1530/acta.0.0970166","title":"Qualitative and quantitative differences in the isoelectrofocusing profile of biologically active lutropin in the blood of normally menstruating and post-menopausal women","display_name":"Qualitative and quantitative differences in the isoelectrofocusing profile of biologically active lutropin in the blood of normally menstruating and post-menopausal women","publication_year":1981,"publication_date":"1981-06-01","ids":{"openalex":"https://openalex.org/W2015120149","doi":"https://doi.org/10.1530/acta.0.0970166","mag":"2015120149","pmid":"https://pubmed.ncbi.nlm.nih.gov/7015773"},"language":"en","primary_location":{"id":"doi:10.1530/acta.0.0970166","doi":"https://doi.org/10.1530/acta.0.0970166","is_oa":false,"landing_page_url":"https://doi.org/10.1530/acta.0.0970166","pdf_url":null,"source":{"id":"https://openalex.org/S4210218062","display_name":"European Journal of Endocrinology","issn_l":"0804-4643","issn":["0804-4643","1479-683X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Endocrinologica","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042464990","display_name":"Felice Strollo","orcid":"https://orcid.org/0000-0001-8917-5314","observed_orcids":["https://orcid.org/0000-0001-8917-5314"]},"institutions":[{"id":"https://openalex.org/I1335113835","display_name":"Karolinska University Hospital","ror":"https://ror.org/00m8d6786","country_code":"SE","type":"healthcare","lineage":["https://openalex.org/I1335113835"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"F. Strollo","raw_affiliation_strings":["Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm","institution_ids":["https://openalex.org/I1335113835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111657859","display_name":"Jonas Harlin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1335113835","display_name":"Karolinska University Hospital","ror":"https://ror.org/00m8d6786","country_code":"SE","type":"healthcare","lineage":["https://openalex.org/I1335113835"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"J. Harlin","raw_affiliation_strings":["Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm","institution_ids":["https://openalex.org/I1335113835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012147227","display_name":"Homero Hern\u00e1ndez-Montes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1335113835","display_name":"Karolinska University Hospital","ror":"https://ror.org/00m8d6786","country_code":"SE","type":"healthcare","lineage":["https://openalex.org/I1335113835"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"H. Hernandez-Montes","raw_affiliation_strings":["Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm","institution_ids":["https://openalex.org/I1335113835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110133059","display_name":"Duncan M. Robertson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1335113835","display_name":"Karolinska University Hospital","ror":"https://ror.org/00m8d6786","country_code":"SE","type":"healthcare","lineage":["https://openalex.org/I1335113835"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"D. M. Robertson","raw_affiliation_strings":["Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm","institution_ids":["https://openalex.org/I1335113835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047548563","display_name":"A. A. Zaidi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1335113835","display_name":"Karolinska University Hospital","ror":"https://ror.org/00m8d6786","country_code":"SE","type":"healthcare","lineage":["https://openalex.org/I1335113835"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"A. A. Zaidi","raw_affiliation_strings":["Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm","institution_ids":["https://openalex.org/I1335113835"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5007846525","display_name":"Egon R. Diczfalusy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1335113835","display_name":"Karolinska University Hospital","ror":"https://ror.org/00m8d6786","country_code":"SE","type":"healthcare","lineage":["https://openalex.org/I1335113835"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"E. Diczfalusy","raw_affiliation_strings":["Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Reproductive Endocrinology Research Unit and Department of Obstetrics and Gynaecology, Karolinska sjukkuset, Stockholm","institution_ids":["https://openalex.org/I1335113835"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1335113835"],"apc_list":{"value":3798,"currency":"EUR","value_usd":4292},"apc_paid":null,"fwci":2.8934,"has_fulltext":false,"cited_by_count":57,"citation_normalized_percentile":{"value":0.9003297,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"97","issue":"2","first_page":"166","last_page":"175"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10960","display_name":"Hypothalamic control of reproductive hormones","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10960","display_name":"Hypothalamic control of reproductive hormones","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/luteinizing-hormone","display_name":"luteinizing hormone","score":0.9559999704360962},{"id":"https://openalex.org/keywords/isoelectric-focusing","display_name":"isoelectric focusing","score":0.7770000100135803},{"id":"https://openalex.org/keywords/postmenopausal-women","display_name":"postmenopausal women","score":0.8119999766349792},{"id":"https://openalex.org/keywords/menstrual-cycle","display_name":"menstrual cycle","score":0.7540000081062317},{"id":"https://openalex.org/keywords/lh-isoforms","display_name":"LH isoforms","score":0.593999981880188},{"id":"https://openalex.org/keywords/lh-bioassay","display_name":"LH bioassay","score":0.6359999775886536}],"concepts":[{"id":"https://openalex.org/C50476208","wikidata":"https://www.wikidata.org/wiki/Q898861","display_name":"Isoelectric focusing","level":3,"score":0.7362247109413147},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.68880295753479},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.6419801712036133},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6181915402412415},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.4945511519908905},{"id":"https://openalex.org/C116073593","wikidata":"https://www.wikidata.org/wiki/Q864491","display_name":"Biological activity","level":3,"score":0.44612589478492737},{"id":"https://openalex.org/C2778562196","wikidata":"https://www.wikidata.org/wiki/Q177708","display_name":"Menopause","level":2,"score":0.4327784478664398},{"id":"https://openalex.org/C3020190219","wikidata":"https://www.wikidata.org/wiki/Q640448","display_name":"Intravenous bolus","level":2,"score":0.42661038041114807},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3809210956096649},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.36330071091651917},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2190006673336029},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.19226905703544617},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16153386235237122},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007525","descriptor_name":"Isoelectric Focusing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007986","descriptor_name":"Luteinizing Hormone","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D007987","descriptor_name":"Gonadotropin-Releasing Hormone","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008593","descriptor_name":"Menopause","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008598","descriptor_name":"Menstruation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010906","descriptor_name":"Pituitary Hormone-Releasing Hormones","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1530/acta.0.0970166","doi":"https://doi.org/10.1530/acta.0.0970166","is_oa":false,"landing_page_url":"https://doi.org/10.1530/acta.0.0970166","pdf_url":null,"source":{"id":"https://openalex.org/S4210218062","display_name":"European Journal of Endocrinology","issn_l":"0804-4643","issn":["0804-4643","1479-683X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Endocrinologica","raw_type":"journal-article"},{"id":"pmid:7015773","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7015773","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta endocrinologica","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320307856","display_name":"World Health Organization","ror":"https://ror.org/01f80g185"},{"id":"https://openalex.org/F4320322581","display_name":"Vetenskapsr\u00e5det","ror":"https://ror.org/03zttf063"},{"id":"https://openalex.org/F4320327561","display_name":"LEO Fondet","ror":"https://ror.org/02rgsr590"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2148346324","https://openalex.org/W2006181143","https://openalex.org/W2023491289","https://openalex.org/W2047846615","https://openalex.org/W2073938953","https://openalex.org/W2026651271","https://openalex.org/W2042089972","https://openalex.org/W2069729167","https://openalex.org/W2090129244","https://openalex.org/W2167201364"],"abstract_inverted_index":{"Abstract.":[0],"A":[1,212,338],"single":[2],"bolus":[3],"of":[4,7,63,152,170,175,181,185,195,204,208,219,227,233,244,250,284,303,315,330,415,429,439,453],"100":[5],"\u03bcg":[6],"gonadoliberin":[8],"(LRH)":[9],"was":[10,23,121,143,221,238,260,295,359,382,409],"administered":[11],"intravenously":[12],"to":[13,51,271,309,334,342],"8":[14],"post-menopausal":[15,234,356,440,463],"and":[16,21,26,30,59,98,135,162,166,207,441,464],"9":[17],"normally":[18,90,103,209,416,442,466],"menstruating":[19,91,104,210,417,443,467],"women":[20,45,92,105,206],"blood":[22,203,438],"withdrawn":[24],"before":[25],"30,":[27],"60,":[28],"120":[29],"240":[31],"min":[32,83],"after":[33,376],"LRH":[34,52,85,319,377,460],"stimulation.":[35],"The":[36,118,140,183,248],"plasma":[37,79,235,246,357,414],"samples":[38,62,232,243,269,281,312,358],"obtained":[39,81],"at":[40,149],"different":[41],"time":[42],"intervals":[43],"from":[44,88,101,241,256,290,388],"showing":[46],"a":[47,94,167,173,216,362,379,393],"sufficiently":[48],"high":[49],"response":[50],"(menopausal:":[53],"8,":[54],"menstruating:":[55],"3)":[56],"were":[57,66,108,324],"combined":[58],"2":[60,102],"ml":[61],"each":[64,124],"pool":[65],"fractionated":[67],"in":[68,110,114,123,129,177,191,201,230,266,278,326,345,349,354,365,369,384,396,400,412,426,435,450,462,465],"triplicate":[69],"by":[70,127,137,361,459],"electrofocusing":[71,116,125],"on":[72],"sucrose":[73],"density":[74],"gradient.":[75],"In":[76],"addition,":[77],"two":[78],"pools,":[80],"30":[82],"following":[84,133,457],"stimulation,":[86],"one":[87],"4":[89],"(exhibiting":[93],"relatively":[95,251,285],"low":[96],"LH-response)":[97],"the":[99,111,178,186,192,196,202,228,267,275,279,282,291,304,310,316,327,335,346,350,355,366,370,385,397,401,413,427,436,451],"other":[100,276],"aged":[106],"40,":[107],"analyzed":[109],"same":[112],"way":[113],"duplicate":[115],"experiments.":[117],"hLH":[119,336,432,455],"activity":[120,142,171],"determined":[122],"fraction":[126],"an":[128],"vitro":[130],"bioassay":[131],"method":[132],"elution":[134],"purification":[136],"gel":[138],"filtration.":[139],"LH":[141],"distributed":[144],"as":[145],"four":[146],"major":[147,423],"peaks":[148,176],"pI":[150,217],"values":[151],"7.10":[153,220],"\u00b1":[154,157,160,164],"0.05,":[155],"7.58":[156],"0.06,":[158],"8.10":[159],"0.04":[161],"8.54":[163],"0.05":[165],"broad":[168],"area":[169],"comprising":[172],"number":[174],"pH":[179,257,292,351,371,389,402],"range":[180,258,293,352,372,390,403],"8.69\u20139.50.":[182,404],"analysis":[184],"data":[187],"revealed":[188],"marked":[189],"differences":[190,424],"relative":[193,328],"distribution":[194],"various":[197,331],"molecular":[198,213,332],"species":[199,214,333,433,456],"present":[200,223,434],"menopausal":[205],"women.":[211,418,444,468],"exhibiting":[215],"value":[218],"invariably":[222],"(10":[224],"\u2013":[225],"15%":[226],"total)":[229],"all":[231,242],"(PMP)":[236],"but":[237],"consistently":[239],"absent":[240],"midcycle":[245],"(MCP).":[247],"amount":[249,283],"'less":[252],"alkaline'":[253,287],"material":[254,288,347,367,386,398],"(eluted":[255],"7.37\u20138.32)":[259],"significantly":[261,296],"higher":[262,300],"(P":[263,297],"<":[264,298],"0.001)":[265,299],"PMP":[268,311],"compared":[270,308],"MCP":[272,280],"samples.":[273],"On":[274],"hand,":[277],"'more":[286],"eluted":[289,348,368,399],"8.33\u20139.50":[294],"(about":[301,313],"60%":[302],"total":[305],"recovered":[306],"activity)":[307],"30%":[314],"total).":[317],"Following":[318],"stimulation":[320,378,458],"significant":[321,380,446],"temporal":[322,447],"changes":[323,448],"observed":[325,411],"contribution":[329],"profile.":[337],"gradual":[339,363],"increase,":[340],"up":[341],"60":[343],"min,":[344],"6.87\u20137.36":[353],"accompanied":[360],"decrease":[364],"7.84\u20138.32.":[373],"Two":[374],"hours":[375],"drop":[381],"found":[383],"collected":[387],"8.33\u20138.68,":[391],"with":[392],"concomitant":[394],"rise":[395],"This":[405],"last":[406],"mentioned":[407],"shift":[408],"also":[410],"It":[419],"is":[420],"concluded":[421],"that":[422],"exist":[425],"composition":[428,452],"biologically":[430],"active":[431],"peripheral":[437],"Moreover,":[445],"occur":[449],"circulating":[454],"both":[461]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}