{"id":"https://openalex.org/W2014581975","doi":"https://doi.org/10.1021/bi0505823","title":"Kinetic, Spectroscopic, and X-ray Crystallographic Characterization of the Functional E151H Aminopeptidase from Aeromonas proteolytica,","display_name":"Kinetic, Spectroscopic, and X-ray Crystallographic Characterization of the Functional E151H Aminopeptidase from Aeromonas proteolytica,","publication_year":2005,"publication_date":"2005-08-19","ids":{"openalex":"https://openalex.org/W2014581975","doi":"https://doi.org/10.1021/bi0505823","mag":"2014581975","pmid":"https://pubmed.ncbi.nlm.nih.gov/16142900"},"language":"en","primary_location":{"id":"doi:10.1021/bi0505823","doi":"https://doi.org/10.1021/bi0505823","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi0505823","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://epublications.marquette.edu/context/physics_fac/article/1034/viewcontent/bennett_6064.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5031350289","display_name":"Krzysztof P. Bzymek","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121980950","display_name":"Utah State University","ror":"https://ror.org/00h6set76","country_code":"US","type":"education","lineage":["https://openalex.org/I121980950"]},{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]},{"id":"https://openalex.org/I6902469","display_name":"Brandeis University","ror":"https://ror.org/05abbep66","country_code":"US","type":"education","lineage":["https://openalex.org/I6902469"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Krzysztof P. Bzymek","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509","institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069872567","display_name":"Aaron G. Moulin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121980950","display_name":"Utah State University","ror":"https://ror.org/00h6set76","country_code":"US","type":"education","lineage":["https://openalex.org/I121980950"]},{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]},{"id":"https://openalex.org/I6902469","display_name":"Brandeis University","ror":"https://ror.org/05abbep66","country_code":"US","type":"education","lineage":["https://openalex.org/I6902469"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aaron Moulin","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509","institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089969812","display_name":"Sabina I. Swierczek","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121980950","display_name":"Utah State University","ror":"https://ror.org/00h6set76","country_code":"US","type":"education","lineage":["https://openalex.org/I121980950"]},{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]},{"id":"https://openalex.org/I6902469","display_name":"Brandeis University","ror":"https://ror.org/05abbep66","country_code":"US","type":"education","lineage":["https://openalex.org/I6902469"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sabina I. Swierczek","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509","institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014900252","display_name":"Dagmar Ringe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121980950","display_name":"Utah State University","ror":"https://ror.org/00h6set76","country_code":"US","type":"education","lineage":["https://openalex.org/I121980950"]},{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]},{"id":"https://openalex.org/I6902469","display_name":"Brandeis University","ror":"https://ror.org/05abbep66","country_code":"US","type":"education","lineage":["https://openalex.org/I6902469"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dagmar Ringe","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509","institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007934367","display_name":"Gregory A. Petsko","orcid":"https://orcid.org/0000-0003-3668-3694","observed_orcids":["https://orcid.org/0000-0003-3668-3694"]},"institutions":[{"id":"https://openalex.org/I121980950","display_name":"Utah State University","ror":"https://ror.org/00h6set76","country_code":"US","type":"education","lineage":["https://openalex.org/I121980950"]},{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]},{"id":"https://openalex.org/I6902469","display_name":"Brandeis University","ror":"https://ror.org/05abbep66","country_code":"US","type":"education","lineage":["https://openalex.org/I6902469"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gregory A. Petsko","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509","institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085194369","display_name":"Brian Bennett","orcid":"https://orcid.org/0000-0001-7795-6260","observed_orcids":["https://orcid.org/0000-0001-7795-6260"]},"institutions":[{"id":"https://openalex.org/I121980950","display_name":"Utah State University","ror":"https://ror.org/00h6set76","country_code":"US","type":"education","lineage":["https://openalex.org/I121980950"]},{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]},{"id":"https://openalex.org/I6902469","display_name":"Brandeis University","ror":"https://ror.org/05abbep66","country_code":"US","type":"education","lineage":["https://openalex.org/I6902469"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian Bennett","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509","institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085500409","display_name":"Richard C. Holz","orcid":"https://orcid.org/0000-0001-6093-2799","observed_orcids":["https://orcid.org/0000-0001-6093-2799"]},"institutions":[{"id":"https://openalex.org/I121980950","display_name":"Utah State University","ror":"https://ror.org/00h6set76","country_code":"US","type":"education","lineage":["https://openalex.org/I121980950"]},{"id":"https://openalex.org/I204308271","display_name":"Medical College of Wisconsin","ror":"https://ror.org/00qqv6244","country_code":"US","type":"education","lineage":["https://openalex.org/I204308271"]},{"id":"https://openalex.org/I6902469","display_name":"Brandeis University","ror":"https://ror.org/05abbep66","country_code":"US","type":"education","lineage":["https://openalex.org/I6902469"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Richard C. Holz","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322-0300, the Program in Biophysics and Structural Biology, Departments of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, Massachusetts 02254, and the National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509","institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5007934367","https://openalex.org/A5085500409"],"corresponding_institution_ids":["https://openalex.org/I121980950","https://openalex.org/I204308271","https://openalex.org/I6902469"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.8341,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.70360656,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"44","issue":"36","first_page":"12030","last_page":"12040"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.7685999870300293,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.7685999870300293,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.07900000363588333,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.027400000020861626,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aminopeptidase","display_name":"aminopeptidase","score":0.9229999780654907},{"id":"https://openalex.org/keywords/aeromonas-proteolytica","display_name":"Aeromonas proteolytica","score":0.8920000195503235},{"id":"https://openalex.org/keywords/zinc-coordination","display_name":"zinc coordination","score":0.5},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.4359999895095825},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.49000000953674316},{"id":"https://openalex.org/keywords/proton-inventory","display_name":"proton inventory","score":0.7850000262260437},{"id":"https://openalex.org/keywords/dppa","display_name":"DPPA","score":0.5059999823570251},{"id":"https://openalex.org/keywords/vibrio-proteolyticus","display_name":"Vibrio proteolyticus","score":0.7889999747276306}],"concepts":[{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.7785593867301941},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7576467990875244},{"id":"https://openalex.org/C2778460671","wikidata":"https://www.wikidata.org/wiki/Q485277","display_name":"Histidine","level":3,"score":0.6719552874565125},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.5855298638343811},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.57530677318573},{"id":"https://openalex.org/C30095370","wikidata":"https://www.wikidata.org/wiki/Q899488","display_name":"Protonation","level":3,"score":0.5243273377418518},{"id":"https://openalex.org/C2776580952","wikidata":"https://www.wikidata.org/wiki/Q483745","display_name":"Leucine","level":3,"score":0.46179699897766113},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.44532105326652527},{"id":"https://openalex.org/C2780985610","wikidata":"https://www.wikidata.org/wiki/Q419527","display_name":"Aminopeptidase","level":4,"score":0.43349170684814453},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.42820560932159424},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.42506420612335205},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.38114285469055176},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.38015472888946533},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16718199849128723},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.14552649855613708},{"id":"https://openalex.org/C145148216","wikidata":"https://www.wikidata.org/wiki/Q36496","display_name":"Ion","level":2,"score":0.12618768215179443}],"mesh":[{"descriptor_ui":"D000626","descriptor_name":"Aminopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000626","descriptor_name":"Aminopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000626","descriptor_name":"Aminopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000626","descriptor_name":"Aminopeptidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013057","descriptor_name":"Spectrum Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016980","descriptor_name":"Aeromonas hydrophila","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D016980","descriptor_name":"Aeromonas hydrophila","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017186","descriptor_name":"Titrimetry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1021/bi0505823","doi":"https://doi.org/10.1021/bi0505823","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi0505823","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:16142900","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16142900","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:epublications.marquette.edu:physics_fac-1034","doi":null,"is_oa":true,"landing_page_url":"https://epublications.marquette.edu/physics_fac/35","pdf_url":"https://epublications.marquette.edu/context/physics_fac/article/1034/viewcontent/bennett_6064.pdf","source":{"id":"https://openalex.org/S4306401682","display_name":"e-Publications@Marquette (Marquette University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102461120","host_organization_name":"Marquette University","host_organization_lineage":["https://openalex.org/I102461120"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Physics Faculty Research and Publications","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:epublications.marquette.edu:physics_fac-1034","doi":null,"is_oa":true,"landing_page_url":"https://epublications.marquette.edu/physics_fac/35","pdf_url":"https://epublications.marquette.edu/context/physics_fac/article/1034/viewcontent/bennett_6064.pdf","source":{"id":"https://openalex.org/S4306401682","display_name":"e-Publications@Marquette (Marquette University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102461120","host_organization_name":"Marquette University","host_organization_lineage":["https://openalex.org/I102461120"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Physics Faculty Research and Publications","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2078910810","display_name":"NATIONAL BIOMEDICAL ESR CENTER","funder_award_id":"P41RR001008","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G6997404690","display_name":"Structural Basis for Bridged Bimetallic Enzyme Catalysis","funder_award_id":"R01GM026788","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2014581975.pdf","grobid_xml":"https://content.openalex.org/works/W2014581975.grobid-xml"},"referenced_works_count":45,"referenced_works":["https://openalex.org/W1188339843","https://openalex.org/W1525055187","https://openalex.org/W1539796472","https://openalex.org/W1557983290","https://openalex.org/W1564876674","https://openalex.org/W1570270309","https://openalex.org/W1756287317","https://openalex.org/W1805898977","https://openalex.org/W1891265320","https://openalex.org/W1967970315","https://openalex.org/W1970289804","https://openalex.org/W1982237156","https://openalex.org/W1982554906","https://openalex.org/W1985446597","https://openalex.org/W1986395837","https://openalex.org/W1986894327","https://openalex.org/W1990559915","https://openalex.org/W1994233757","https://openalex.org/W1996630033","https://openalex.org/W2001151163","https://openalex.org/W2017370081","https://openalex.org/W2033090934","https://openalex.org/W2035861138","https://openalex.org/W2040013605","https://openalex.org/W2042614258","https://openalex.org/W2043835205","https://openalex.org/W2045918025","https://openalex.org/W2046137490","https://openalex.org/W2047514291","https://openalex.org/W2065320301","https://openalex.org/W2075103281","https://openalex.org/W2077294102","https://openalex.org/W2078317461","https://openalex.org/W2083676460","https://openalex.org/W2085217535","https://openalex.org/W2089808128","https://openalex.org/W2113201292","https://openalex.org/W2127132427","https://openalex.org/W2151412416","https://openalex.org/W2161743998","https://openalex.org/W2165350323","https://openalex.org/W2477377300","https://openalex.org/W4211062846","https://openalex.org/W4233869395","https://openalex.org/W4249231186"],"related_works":["https://openalex.org/W1982237156","https://openalex.org/W2064018696","https://openalex.org/W134496009","https://openalex.org/W1971762245","https://openalex.org/W2005399116","https://openalex.org/W2078317461","https://openalex.org/W7033415189","https://openalex.org/W2008992849","https://openalex.org/W1979619881","https://openalex.org/W1999634936"],"abstract_inverted_index":{"Glutamate151":[0],"(E151)":[1],"has":[2,85,244],"been":[3,86,245],"shown":[4],"to":[5,70,92,142,145,204,247,255,264,307],"be":[6],"catalytically":[7],"essential":[8],"for":[9,47,78,182],"the":[10,19,23,35,54,75,94,156,167,176,183,197,214,225,231,235,238,265,270,282,294,308,312],"aminopeptidase":[11],"from":[12,56,200],"Vibrio":[13],"proteolyticus":[14],"(AAP).":[15],"E151":[16,71,103,210,254,297],"acts":[17],"as":[18,41,88,281],"general":[20,43,216,283],"acid/base":[21,44,284],"during":[22,45],"catalytic":[24],"mechanism":[25],"of":[26,39,53,97,102,116,151,175,185,227,234,242,253,288,293,296,311],"peptide":[27],"hydrolysis.":[28],"However,":[29],"a":[30,42,61,89,113,162,190,276,305],"glutamate":[31],"residue":[32,37,63,278],"is":[33,120,171],"not":[34,258],"only":[36],"capable":[38],"functioning":[40],"catalysis":[46],"dinuclear":[48,271],"metallohydrolases.":[49],"Recent":[50],"crystallographic":[51],"characterization":[52],"D-aminopeptidase":[55],"Bacillus":[57],"subtilis":[58],"(DppA)":[59],"revealed":[60,132,189],"histidine":[62,105,256,277],"that":[64,133,155],"resides":[65,160],"in":[66,72,99,107,161,192,196,202,206,211,230,285,298,301],"an":[67,108,193],"identical":[68],"position":[69],"AAP.":[73],"Because":[74],"active-site":[76,215],"ligands":[77],"DppA":[79,302],"and":[80,137,148,180,251],"AAP":[81,84,110,127,212],"are":[82],"identical,":[83],"used":[87],"model":[90],"enzyme":[91,111],"understand":[93],"mechanistic":[95],"role":[96,295],"H115":[98,300],"DppA.":[100],"Substitution":[101],"with":[104,209],"resulted":[106],"active":[109,273],"exhibiting":[112],"kcat":[114,179],"value":[115],"2.0":[117],"min(-1),":[118],"which":[119],"over":[121],"2000":[122],"times":[123,139],"slower":[124],"than":[125],"r":[126],"(4380":[128],"min(-1)).":[129],"ITC":[130],"experiments":[131],"ZnII":[134,272],"binds":[135],"330":[136],"3":[138],"more":[140],"weakly":[141],"E151H-AAP":[143,153],"compared":[144],"r-AAP.":[146],"UV-vis":[147],"EPR":[149],"spectra":[150],"CoII-loaded":[152],"indicated":[154],"first":[157],"metal":[158,169],"ion":[159,170],"hexacoordinate/pentacoordinate":[163],"equilibrium":[164],"environment,":[165],"whereas":[166],"second":[168],"six-coordinate.":[172],"pH":[173,222],"dependence":[174],"kinetic":[177],"parameters":[178],"K(m)":[181],"hydrolysis":[184,286],"L-leucine":[186],"p-nitroanilide":[187],"(L-pNA)":[188],"change":[191],"ionization":[194],"constant":[195],"enzyme-substrate":[198],"complex":[199],"5.3":[201],"r-AAP":[203],"6.4":[205],"E151H-AAP,":[207],"consistent":[208],"being":[213],"acid/base.":[217],"Proton":[218],"inventory":[219],"studies":[220],"at":[221],"8.50":[223],"indicate":[224],"transfer":[226],"one":[228],"proton":[229,306],"rate-limiting":[232],"step":[233],"reaction.":[236],"Moreover,":[237],"X-ray":[239],"crystal":[240],"structure":[241,268],"[ZnZn(E151H-AAP)]":[243],"solved":[246],"1.9":[248],"A":[249],"resolution,":[250],"alteration":[252],"does":[257],"introduce":[259],"any":[260],"major":[261],"conformational":[262],"changes":[263],"overall":[266],"protein":[267],"or":[269],"site.":[274],"Therefore,":[275],"can":[279],"function":[280],"reactions":[287],"peptides":[289],"and,":[290],"through":[291],"analogy":[292],"AAP,":[299],"likely":[303],"shuttles":[304],"leaving":[309],"group":[310],"substrate.":[313]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}