{"id":"https://openalex.org/W2014545488","doi":"https://doi.org/10.1002/stem.5530070505","title":"Microcapillary clonogenic assays for human marrow hematopoietic progenitor cells","display_name":"Microcapillary clonogenic assays for human marrow hematopoietic progenitor cells","publication_year":1989,"publication_date":"1989-01-01","ids":{"openalex":"https://openalex.org/W2014545488","doi":"https://doi.org/10.1002/stem.5530070505","mag":"2014545488","pmid":"https://pubmed.ncbi.nlm.nih.gov/2768844"},"language":"en","primary_location":{"id":"doi:10.1002/stem.5530070505","doi":"https://doi.org/10.1002/stem.5530070505","is_oa":false,"landing_page_url":"https://doi.org/10.1002/stem.5530070505","pdf_url":null,"source":{"id":"https://openalex.org/S4210239040","display_name":"The International Journal Of Cell Cloning","issn_l":"0737-1454","issn":["0737-1454"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The International Journal of Cell Cloning","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110396195","display_name":"De\u2010Lin Du","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210091357","display_name":"Hipple Cancer Research Center","ror":"https://ror.org/00e4xmg89","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210091357"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"De\u2010Lin Du","raw_affiliation_strings":["Hippie Cancer Research Center, Dayton, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hippie Cancer Research Center, Dayton, Ohio, USA","institution_ids":["https://openalex.org/I4210091357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067664883","display_name":"Donna A. Volpe","orcid":"https://orcid.org/0000-0002-8286-9679","observed_orcids":["https://orcid.org/0000-0002-8286-9679"]},"institutions":[{"id":"https://openalex.org/I4210091357","display_name":"Hipple Cancer Research Center","ror":"https://ror.org/00e4xmg89","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210091357"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donna A. Volpe","raw_affiliation_strings":["Hippie Cancer Research Center, Dayton, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hippie Cancer Research Center, Dayton, Ohio, USA","institution_ids":["https://openalex.org/I4210091357"]}]},{"author_position":"last","author":{"id":null,"display_name":"Martin J. Murphy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210091357","display_name":"Hipple Cancer Research Center","ror":"https://ror.org/00e4xmg89","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210091357"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martin J. Murphy","raw_affiliation_strings":["Hippie Cancer Research Center, Dayton, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hippie Cancer Research Center, Dayton, Ohio, USA","institution_ids":["https://openalex.org/I4210091357"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210091357"],"apc_list":null,"apc_paid":null,"fwci":0.7072,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.69047059,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"7","issue":"5","first_page":"303","last_page":"313"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12919","display_name":"Blood disorders and treatments","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hematopoietic-progenitor-cells","display_name":"hematopoietic progenitor cells","score":0.9229999780654907},{"id":"https://openalex.org/keywords/human-bone-marrow","display_name":"human bone marrow","score":0.902999997138977},{"id":"https://openalex.org/keywords/cfu-gm","display_name":"CFU-GM","score":0.9179999828338623},{"id":"https://openalex.org/keywords/cfu-e","display_name":"CFU-E","score":0.9760000109672546},{"id":"https://openalex.org/keywords/bfu-e","display_name":"BFU-E","score":0.9860000014305115},{"id":"https://openalex.org/keywords/erythroid-colony-formation","display_name":"erythroid colony formation","score":0.7540000081062317},{"id":"https://openalex.org/keywords/myelosuppression","display_name":"myelosuppression","score":0.7879999876022339},{"id":"https://openalex.org/keywords/chemotherapy-induced-bone-marrow-toxicity","display_name":"chemotherapy-induced bone marrow toxicity","score":0.9620000123977661},{"id":"https://openalex.org/keywords/anticancer-agents","display_name":"anticancer agents","score":0.7210000157356262},{"id":"https://openalex.org/keywords/anti-hiv-agents","display_name":"anti-HIV agents","score":0.9169999957084656},{"id":"https://openalex.org/keywords/plating-efficiency","display_name":"plating efficiency","score":0.7459999918937683}],"concepts":[{"id":"https://openalex.org/C117262875","wikidata":"https://www.wikidata.org/wiki/Q5135057","display_name":"Clonogenic assay","level":3,"score":0.8790107369422913},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.7975846529006958},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.6356503367424011},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.5722083449363708},{"id":"https://openalex.org/C2777279920","wikidata":"https://www.wikidata.org/wiki/Q2931384","display_name":"CFU-GM","level":4,"score":0.5347691178321838},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.445297509431839},{"id":"https://openalex.org/C146763376","wikidata":"https://www.wikidata.org/wiki/Q81714","display_name":"Petri dish","level":2,"score":0.43187129497528076},{"id":"https://openalex.org/C2779282312","wikidata":"https://www.wikidata.org/wiki/Q1956561","display_name":"Myeloid","level":2,"score":0.4166441261768341},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.40539494156837463},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3745283782482147},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3324376046657562},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.3210405707359314},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.28295716643333435},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.20515978336334229},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.05534470081329346},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001854","descriptor_name":"Bone Marrow Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003114","descriptor_name":"Colony-Forming Units Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/stem.5530070505","doi":"https://doi.org/10.1002/stem.5530070505","is_oa":false,"landing_page_url":"https://doi.org/10.1002/stem.5530070505","pdf_url":null,"source":{"id":"https://openalex.org/S4210239040","display_name":"The International Journal Of Cell Cloning","issn_l":"0737-1454","issn":["0737-1454"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The International Journal of Cell Cloning","raw_type":"journal-article"},{"id":"pmid:2768844","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2768844","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of cell cloning","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9763000011444092}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W1508083554","https://openalex.org/W1919567759","https://openalex.org/W1998276307","https://openalex.org/W2051253120","https://openalex.org/W2074445204","https://openalex.org/W2103883868","https://openalex.org/W2240632672","https://openalex.org/W2410989579","https://openalex.org/W2418342176","https://openalex.org/W2423979659","https://openalex.org/W2466484359"],"related_works":["https://openalex.org/W2033367628","https://openalex.org/W31822687","https://openalex.org/W2041907799","https://openalex.org/W1976582271","https://openalex.org/W2335899131","https://openalex.org/W2040259461","https://openalex.org/W4253894018","https://openalex.org/W2050254443","https://openalex.org/W2473526971","https://openalex.org/W178868619"],"abstract_inverted_index":{"The":[0,20,81],"capillary":[1],"clonogenic":[2],"cell":[3],"assay":[4,83],"was":[5],"developed":[6,85],"and":[7,12,34,42,62,86,116,119],"adapted":[8],"to":[9,98],"culture":[10,55],"myeloid":[11],"erythroid":[13,30,35],"colonies":[14],"from":[15,76],"human":[16,77],"bone":[17,25,46,78,128],"marrow":[18,26,47,79,129],"cells.":[19,103],"plating":[21],"efficiencies":[22],"for":[23,68,108,120],"femoral":[24],"granulocyte-macrophage":[27],"progenitors":[28],"(CFU-gm),":[29],"colony-forming":[31],"units":[32,37],"(CFU-e)":[33],"burst-forming":[36],"(BFU-e)":[38],"were":[39],"0.143%,":[40],"0.229%":[41],"0.141%,":[43],"respectively.":[44],"Standard":[45],"progenitor":[48],"Petri":[49],"dish":[50],"assays":[51],"require":[52],"a":[53],"total":[54],"volume":[56],"of":[57,72,95,102,112,123],"1":[58],"ml":[59],"per":[60],"dish,":[61],"as":[63,84],"such":[64],"are":[65],"not":[66],"suitable":[67,107],"the":[69,92,110,124],"small":[70,100],"numbers":[71,101],"cells":[73],"often":[74],"obtained":[75],"samples.":[80],"microcapillary":[82],"standardized":[87],"in":[88],"our":[89],"laboratory":[90],"has":[91],"unique":[93],"advantage":[94],"being":[96],"able":[97],"utilize":[99],"This":[104],"technique":[105],"is":[106],"evaluating":[109],"myelotoxicity":[111],"investigational":[113],"new":[114],"anti-cancer":[115],"anti-HIV":[117],"agents":[118],"further":[121],"investigation":[122],"mechanisms":[125],"underlying":[126],"chemotherapy-induced":[127],"toxicity.":[130]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}