{"id":"https://openalex.org/W2014529982","doi":"https://doi.org/10.1093/nar/19.8.1831","title":"A new method for the rapid identification of genes encoding restriction and modification enzymes","display_name":"A new method for the rapid identification of genes encoding restriction and modification enzymes","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W2014529982","doi":"https://doi.org/10.1093/nar/19.8.1831","mag":"2014529982","pmid":"https://pubmed.ncbi.nlm.nih.gov/1851562","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/328112"},"language":"en","primary_location":{"id":"doi:10.1093/nar/19.8.1831","doi":"https://doi.org/10.1093/nar/19.8.1831","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/19.8.1831","pdf_url":"https://academic.oup.com/nar/article-pdf/19/8/1831/7059642/19-8-1831.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/nar/article-pdf/19/8/1831/7059642/19-8-1831.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021275086","display_name":"Andrzej Piekarowicz","orcid":"https://orcid.org/0000-0003-4394-3044","observed_orcids":["https://orcid.org/0000-0003-4394-3044"]},"institutions":[{"id":"https://openalex.org/I4654613","display_name":"University of Warsaw","ror":"https://ror.org/039bjqg32","country_code":"PL","type":"education","lineage":["https://openalex.org/I4654613"]},{"id":"https://openalex.org/I66946132","display_name":"University of Maryland, College Park","ror":"https://ror.org/047s2c258","country_code":"US","type":"education","lineage":["https://openalex.org/I66946132"]}],"countries":["PL","US"],"is_corresponding":false,"raw_author_name":"Andrzej Piekarowicz","raw_affiliation_strings":["Institute of Microbiology, Warsaw University, Poland","Department of Microbiology, University of MarylandCollege Park, MD 20742, USA","Institute of Microbiology, Warsaw UniversityNowy Swiat 67, 00-046 Warsaw, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Microbiology, Warsaw University, Poland","institution_ids":["https://openalex.org/I4654613"]},{"raw_affiliation_string":"Department of Microbiology, University of MarylandCollege Park, MD 20742, USA","institution_ids":["https://openalex.org/I66946132"]},{"raw_affiliation_string":"Institute of Microbiology, Warsaw UniversityNowy Swiat 67, 00-046 Warsaw, Poland","institution_ids":["https://openalex.org/I4654613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103467410","display_name":"Robert T. Yuan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I66946132","display_name":"University of Maryland, College Park","ror":"https://ror.org/047s2c258","country_code":"US","type":"education","lineage":["https://openalex.org/I66946132"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert Yuan","raw_affiliation_strings":["Department of Microbiology, University of Maryland College Park, MD 20742, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Maryland College Park, MD 20742, USA","institution_ids":["https://openalex.org/I66946132"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079145539","display_name":"Daniel C. Stein","orcid":"https://orcid.org/0000-0003-3397-5720","observed_orcids":["https://orcid.org/0000-0003-3397-5720"]},"institutions":[{"id":"https://openalex.org/I66946132","display_name":"University of Maryland, College Park","ror":"https://ror.org/047s2c258","country_code":"US","type":"education","lineage":["https://openalex.org/I66946132"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Daniel C. Stein","raw_affiliation_strings":["Department of Microbiology, University of Maryland College Park, MD 20742, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Maryland College Park, MD 20742, USA","institution_ids":["https://openalex.org/I66946132"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5079145539"],"corresponding_institution_ids":["https://openalex.org/I66946132"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":1.4705,"has_fulltext":true,"cited_by_count":31,"citation_normalized_percentile":{"value":0.80367995,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"19","issue":"8","first_page":"1831","last_page":"1835"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9434000253677368,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9434000253677368,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.035100001841783524,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.005900000222027302,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/restriction-enzymes","display_name":"restriction enzymes","score":0.7070000171661377},{"id":"https://openalex.org/keywords/dna-methyltransferases","display_name":"DNA methyltransferases","score":0.9900000095367432},{"id":"https://openalex.org/keywords/gene-identification","display_name":"gene identification","score":0.7649999856948853},{"id":"https://openalex.org/keywords/escherichia-coli-mutants","display_name":"Escherichia coli mutants","score":0.7409999966621399},{"id":"https://openalex.org/keywords/sos-response","display_name":"SOS response","score":0.9829999804496765},{"id":"https://openalex.org/keywords/lacz-fusion","display_name":"lacZ fusions","score":0.5979999899864197},{"id":"https://openalex.org/keywords/temperature-sensitive-mutants","display_name":"temperature-sensitive mutants","score":0.6359999775886536},{"id":"https://openalex.org/keywords/methylated-dna","display_name":"methylated DNA","score":0.6359999775886536},{"id":"https://openalex.org/keywords/plasmid-selection","display_name":"plasmid selection","score":0.7329999804496765},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.3930000066757202}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8798633813858032},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.7410444021224976},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6319100260734558},{"id":"https://openalex.org/C91965660","wikidata":"https://www.wikidata.org/wiki/Q415875","display_name":"Methyltransferase","level":4,"score":0.6198037266731262},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5889835357666016},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5343387126922607},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.5174177885055542},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5038792490959167},{"id":"https://openalex.org/C2779979206","wikidata":"https://www.wikidata.org/wiki/Q1154602","display_name":"DNA methyltransferase","level":5,"score":0.4764462113380432},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4659845232963562},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4063996970653534},{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.049785733222961426}],"mesh":[{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004249","descriptor_name":"DNA Damage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005821","descriptor_name":"Genetic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013014","descriptor_name":"SOS Response, Genetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014169","descriptor_name":"Transformation, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015254","descriptor_name":"DNA Modification Methylases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/19.8.1831","doi":"https://doi.org/10.1093/nar/19.8.1831","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/19.8.1831","pdf_url":"https://academic.oup.com/nar/article-pdf/19/8/1831/7059642/19-8-1831.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:328112","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/328112","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:1851562","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1851562","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.958.5507","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.958.5507","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://nar.oxfordjournals.org/content/19/8/1831.full.pdf","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1093/nar/19.8.1831","doi":"https://doi.org/10.1093/nar/19.8.1831","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/19.8.1831","pdf_url":"https://academic.oup.com/nar/article-pdf/19/8/1831/7059642/19-8-1831.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G101863322","display_name":null,"funder_award_id":"AI24452","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2014529982.pdf","grobid_xml":"https://content.openalex.org/works/W2014529982.grobid-xml"},"referenced_works_count":10,"referenced_works":["https://openalex.org/W1513717105","https://openalex.org/W1973670295","https://openalex.org/W2019410656","https://openalex.org/W2049228498","https://openalex.org/W2050499271","https://openalex.org/W2059545334","https://openalex.org/W2064543440","https://openalex.org/W2126994787","https://openalex.org/W4251861560","https://openalex.org/W6613905171"],"related_works":["https://openalex.org/W2064543440","https://openalex.org/W936028792","https://openalex.org/W1984368904","https://openalex.org/W1976300710","https://openalex.org/W2073287325","https://openalex.org/W1971388491","https://openalex.org/W7099983566","https://openalex.org/W1968427082","https://openalex.org/W2045838004","https://openalex.org/W2409816589"],"abstract_inverted_index":{"We":[0],"have":[1,59,70,140],"constructed":[2,60],"derivatives":[3],"of":[4,15,26,41,54,85,90,115,144],"Escherichia":[5],"coli":[6,56],"that":[7,57,105],"can":[8],"be":[9],"used":[10,45],"for":[11,63],"the":[12,27,33,39,64,81,109,112,116],"rapid":[13],"identification":[14],"recombinant":[16,91],"plasmids":[17,48,92],"encoding":[18,49,93,120],"DNA":[19,50,94,121,145],"restriction":[20,97],"enzymes":[21,98],"and":[22,35,66,69,96,158],"methyltransferases.":[23,51],"The":[24,52,88],"induction":[25,110],"DNA-damage":[28],"inducible":[29],"SOS":[30],"response":[31],"by":[32],"Mcr":[34,65],"Mrr":[36,67],"systems,":[37],"in":[38],"presence":[40],"methylated":[42],"DNA,":[43],"is":[44,99,106],"to":[46,74,80],"select":[47],"strains":[53],"E.":[55,86],"we":[58,139],"are":[61,124],"temperature-sensitive":[62],"systems":[68],"been":[71],"further":[72],"modified":[73],"include":[75],"a":[76,100,142],"lacZ":[77,117],"gene":[78],"fused":[79],"damage-inducible":[82],"dinD":[83],"locus":[84],"coli.":[87],"detection":[89],"methyltransferases":[95],"simple,":[101],"one":[102],"step":[103],"procedure":[104],"based":[107],"on":[108,126],"at":[111],"restrictive":[113],"temperature":[114],"gene.":[118],"Transformants":[119],"methyltransferase":[122,146],"genes":[123,147],"detected":[125],"LB":[127],"agar":[128],"plates":[129],"supplemented":[130],"with":[131],"X-gal":[132],"as":[133,152],"blue":[134],"colonies.":[135],"Using":[136],"this":[137],"method,":[138],"cloned":[141],"variety":[143],"from":[148],"diverse":[149],"species":[150],"such":[151],"Neisseria,":[153],"Haemophilus,":[154],"Treponema,":[155],"Pseudomonas,":[156],"Xanthomonas":[157],"Saccharopolyspora.":[159]},"counts_by_year":[{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
