{"id":"https://openalex.org/W2014527780","doi":"https://doi.org/10.1590/s0100-41582004000500017","title":"Polyclonal antibodies to the coat protein of Apple stem grooving virus expressed in Escherichia coli: production and use in immunodiagnosis","display_name":"Polyclonal antibodies to the coat protein of Apple stem grooving virus expressed in Escherichia coli: production and use in immunodiagnosis","publication_year":2004,"publication_date":"2004-10-01","ids":{"openalex":"https://openalex.org/W2014527780","doi":"https://doi.org/10.1590/s0100-41582004000500017","mag":"2014527780"},"language":"en","primary_location":{"id":"doi:10.1590/s0100-41582004000500017","doi":"https://doi.org/10.1590/s0100-41582004000500017","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0100-41582004000500017","pdf_url":"https://www.scielo.br/j/fb/a/fv4h3mHsLvZJQZPdgqDLMwN/?lang=en&format=pdf","source":{"id":"https://openalex.org/S85248228","display_name":"Fitopatologia Brasileira","issn_l":"0100-4158","issn":["0100-4158","1678-4677"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fitopatologia Brasileira","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.scielo.br/j/fb/a/fv4h3mHsLvZJQZPdgqDLMwN/?lang=en&format=pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011442820","display_name":"Osmar Nickel","orcid":"https://orcid.org/0000-0002-7969-648X","observed_orcids":["https://orcid.org/0000-0002-7969-648X"]},"institutions":[{"id":"https://openalex.org/I4405282979","display_name":"Embrapa Uva e Vinho","ror":"https://ror.org/00sdy1r75","country_code":"BR","type":"government","lineage":["https://openalex.org/I199691007","https://openalex.org/I4405282979"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Osmar Nickel","raw_affiliation_strings":["Embrapa Uva e Vinho"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Embrapa Uva e Vinho","institution_ids":["https://openalex.org/I4405282979"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113664655","display_name":"Maria Lu\u00edsa P.N. Targon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Maria L.P.N. Targon","raw_affiliation_strings":["Centro Avan\u00e7ado de Pesquisa Tecnol\u00f3gica do Agroneg\u00f3cio de Citros Sylvio Moreira"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro Avan\u00e7ado de Pesquisa Tecnol\u00f3gica do Agroneg\u00f3cio de Citros Sylvio Moreira","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077111877","display_name":"T. V. M. Fajardo","orcid":"https://orcid.org/0000-0001-6621-5192","observed_orcids":["https://orcid.org/0000-0001-6621-5192"]},"institutions":[{"id":"https://openalex.org/I4405282979","display_name":"Embrapa Uva e Vinho","ror":"https://ror.org/00sdy1r75","country_code":"BR","type":"government","lineage":["https://openalex.org/I199691007","https://openalex.org/I4405282979"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Thor V.M. Fajardo","raw_affiliation_strings":["Embrapa Uva e Vinho"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Embrapa Uva e Vinho","institution_ids":["https://openalex.org/I4405282979"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080959286","display_name":"Marcos Ant\u00f4nio Machado","orcid":"https://orcid.org/0000-0001-9780-3990","observed_orcids":["https://orcid.org/0000-0001-9780-3990"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Marcos A. Machado","raw_affiliation_strings":["Centro Avan\u00e7ado de Pesquisa Tecnol\u00f3gica do Agroneg\u00f3cio de Citros Sylvio Moreira"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro Avan\u00e7ado de Pesquisa Tecnol\u00f3gica do Agroneg\u00f3cio de Citros Sylvio Moreira","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024079116","display_name":"Ana Paula Trivilin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210158017","display_name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","ror":"https://ror.org/05k49za97","country_code":"BR","type":"government","lineage":["https://openalex.org/I4210099365","https://openalex.org/I4210158017"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Ana P. Trivilin","raw_affiliation_strings":["Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","institution_ids":["https://openalex.org/I4210158017"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4125,"has_fulltext":true,"cited_by_count":32,"citation_normalized_percentile":{"value":0.71403371,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"29","issue":"5","first_page":"558","last_page":"562"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11750","display_name":"Phytoplasmas and Hemiptera pathogens","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/apple-stem-grooving-virus","display_name":"apple stem grooving virus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/capsid-protein","display_name":"capsid protein","score":0.968999981880188},{"id":"https://openalex.org/keywords/polyclonal-antibodies","display_name":"polyclonal antibodies","score":0.9909999966621399},{"id":"https://openalex.org/keywords/maltose-binding-protein-fusion-protein","display_name":"maltose-binding protein fusion","score":0.9240000247955322},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.777999997138977},{"id":"https://openalex.org/keywords/immunodiagnosis","display_name":"immunodiagnosis","score":0.824999988079071},{"id":"https://openalex.org/keywords/elisa","display_name":"ELISA","score":0.8529999852180481},{"id":"https://openalex.org/keywords/apple","display_name":"apple","score":0.7770000100135803},{"id":"https://openalex.org/keywords/quinoa","display_name":"quinoa","score":0.9290000200271606},{"id":"https://openalex.org/keywords/virus-detection","display_name":"virus detection","score":0.42100000381469727}],"concepts":[{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.830244779586792},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7299063205718994},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.7051278948783875},{"id":"https://openalex.org/C2780676925","wikidata":"https://www.wikidata.org/wiki/Q24741276","display_name":"Maltose-binding protein","level":5,"score":0.6157320141792297},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5255721211433411},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.5207114815711975},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5204392075538635},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.5115535855293274},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.44768428802490234},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.3405751585960388},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.2854386568069458},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1941983699798584},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19178521633148193},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1590/s0100-41582004000500017","doi":"https://doi.org/10.1590/s0100-41582004000500017","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0100-41582004000500017","pdf_url":"https://www.scielo.br/j/fb/a/fv4h3mHsLvZJQZPdgqDLMwN/?lang=en&format=pdf","source":{"id":"https://openalex.org/S85248228","display_name":"Fitopatologia Brasileira","issn_l":"0100-4158","issn":["0100-4158","1678-4677"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fitopatologia Brasileira","raw_type":"journal-article"},{"id":"pmh:oai:scielo:S0100-41582004000500017","doi":null,"is_oa":true,"landing_page_url":"http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500017","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fitopatologia Brasileira v.29 n.5 2004","raw_type":"info:eu-repo/semantics/report"},{"id":"pmh:oai:americanae.aecid.es:2693404","doi":null,"is_oa":true,"landing_page_url":"http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500017","pdf_url":null,"source":{"id":"https://openalex.org/S4306400786","display_name":"Americanae (AECID Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Fitopatologia Brasileira v.29 n.5 2004","raw_type":"info:eu-repo/semantics/report"}],"best_oa_location":{"id":"doi:10.1590/s0100-41582004000500017","doi":"https://doi.org/10.1590/s0100-41582004000500017","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0100-41582004000500017","pdf_url":"https://www.scielo.br/j/fb/a/fv4h3mHsLvZJQZPdgqDLMwN/?lang=en&format=pdf","source":{"id":"https://openalex.org/S85248228","display_name":"Fitopatologia Brasileira","issn_l":"0100-4158","issn":["0100-4158","1678-4677"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fitopatologia Brasileira","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8409000039100647,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322502","display_name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","ror":"https://ror.org/05k49za97"},{"id":"https://openalex.org/F4320322904","display_name":"Financiadora de Estudos e Projetos","ror":"https://ror.org/030w99567"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2014527780.pdf","grobid_xml":"https://content.openalex.org/works/W2014527780.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W1684880136","https://openalex.org/W1972934647","https://openalex.org/W2003511339","https://openalex.org/W2006454438","https://openalex.org/W2018767475","https://openalex.org/W2032560420","https://openalex.org/W2046303933","https://openalex.org/W2061437260","https://openalex.org/W2065818024","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2132417094","https://openalex.org/W2144634347","https://openalex.org/W2149249186","https://openalex.org/W2314225431","https://openalex.org/W2913136518"],"related_works":["https://openalex.org/W2995854674","https://openalex.org/W6963697566","https://openalex.org/W2276053872","https://openalex.org/W2321146979","https://openalex.org/W1991018140","https://openalex.org/W1981675315","https://openalex.org/W2589496331","https://openalex.org/W2000767044","https://openalex.org/W2086021292","https://openalex.org/W2069590029"],"abstract_inverted_index":{"The":[0,34,111,125],"coat":[1,36],"protein":[2,37,44,53,65,81,114],"gene":[3],"of":[4,48,100,148,160],"Apple":[5],"stem":[6],"grooving":[7],"virus":[8],"(ASGV)":[9],"was":[10,25,39,66],"amplified":[11],"by":[12,59,68],"RT-PCR,":[13],"cloned,":[14],"sequenced":[15],"and":[16,61,95,104,133,151,155,162],"subcloned":[17],"in":[18,107,122,131,158],"the":[19,62,79,136,145],"expression":[20],"vector":[21],"pMal-c2.":[22],"This":[23,138],"plasmid":[24],"used":[26],"to":[27,85,102,116,135],"transform":[28],"Escherichia":[29],"coli":[30,50],"BL21c+":[31],"competent":[32],"cells.":[33],"ASGV":[35,86,121],"(cp)":[38],"expressed":[40],"as":[41],"a":[42,46,117],"fusion":[43,64,80,113],"containing":[45],"fragment":[47],"E.":[49],"maltose":[51],"binding":[52],"(MBP).":[54],"Bacterial":[55],"cells":[56],"were":[57],"disrupted":[58],"sonication":[60],"ASGVcp/MBP":[63,112,128],"purified":[67],"amylose":[69],"resin":[70],"affinity":[71],"chromatography.":[72],"Polyclonal":[73],"antibodies":[74],"from":[75,87],"rabbits":[76],"immunized":[77],"with":[78],"gave":[82],"specific":[83],"reactions":[84],"infected":[88],"apple":[89,149],"(Malus":[90],"domestica)":[91],"cv.":[92],"Fuji":[93],"Irradiada":[94],"Chenopodium":[96],"quinoa":[97],"at":[98],"dilutions":[99],"up":[101],"1:1,000":[103],"1:2,000,":[105],"respectively,":[106],"plate":[108],"trapped":[109],"ELISA.":[110],"reacted":[115],"commercial":[118],"antiserum":[119],"against":[120,127],"immunoblotting":[123],"assay.":[124],"IgG":[126],"performed":[129],"favorably":[130],"specificity":[132],"sensitivity":[134],"virus.":[137],"method":[139],"represents":[140],"an":[141],"additional":[142],"tool":[143],"for":[144,156],"efficient":[146],"ASGV-indexing":[147],"propagative":[150],"mother":[152],"stock":[153],"materials,":[154],"use":[157],"support":[159],"biological":[161],"molecular":[163],"techniques.":[164]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}