{"id":"https://openalex.org/W2014386676","doi":"https://doi.org/10.1006/abio.1995.1276","title":"Increase in Fluorescence upon the Hydrolysis of Tyrosine Peptides: Application to Proteinase Assays","display_name":"Increase in Fluorescence upon the Hydrolysis of Tyrosine Peptides: Application to Proteinase Assays","publication_year":1995,"publication_date":"1995-05-01","ids":{"openalex":"https://openalex.org/W2014386676","doi":"https://doi.org/10.1006/abio.1995.1276","mag":"2014386676","pmid":"https://pubmed.ncbi.nlm.nih.gov/7668386"},"language":"en","primary_location":{"id":"doi:10.1006/abio.1995.1276","doi":"https://doi.org/10.1006/abio.1995.1276","is_oa":false,"landing_page_url":"https://doi.org/10.1006/abio.1995.1276","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028004629","display_name":"Anne G. Peranteau","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A.G. Peranteau","raw_affiliation_strings":["School of Pharmacy, University of Wisconsin, Madison 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmacy, University of Wisconsin, Madison 53706, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008377738","display_name":"Petr Kuzmi\u010d","orcid":"https://orcid.org/0000-0001-5250-8381","observed_orcids":["https://orcid.org/0000-0001-5250-8381"]},"institutions":[{"id":"https://openalex.org/I1304256225","display_name":"University of Wisconsin System","ror":"https://ror.org/03ydkyb10","country_code":"US","type":"education","lineage":["https://openalex.org/I1304256225"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. Kuzmic","raw_affiliation_strings":["Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA","institution_ids":["https://openalex.org/I1304256225","https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016164212","display_name":"Yvonne M. Angell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1304256225","display_name":"University of Wisconsin System","ror":"https://ror.org/03ydkyb10","country_code":"US","type":"education","lineage":["https://openalex.org/I1304256225"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Y. Angell","raw_affiliation_strings":["Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA","institution_ids":["https://openalex.org/I1304256225","https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006321055","display_name":"Carlos Garc\u0131\u0301a-Echeverr\u0131\u0301a","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1304256225","display_name":"University of Wisconsin System","ror":"https://ror.org/03ydkyb10","country_code":"US","type":"education","lineage":["https://openalex.org/I1304256225"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Garciaecheverria","raw_affiliation_strings":["Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA","institution_ids":["https://openalex.org/I1304256225","https://openalex.org/I135310074"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069015659","display_name":"Daniel H. Rich","orcid":"https://orcid.org/0000-0001-5662-6529","observed_orcids":["https://orcid.org/0000-0001-5662-6529"]},"institutions":[{"id":"https://openalex.org/I1304256225","display_name":"University of Wisconsin System","ror":"https://ror.org/03ydkyb10","country_code":"US","type":"education","lineage":["https://openalex.org/I1304256225"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D.H. Rich","raw_affiliation_strings":["Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ Wisconsin, Sch Pharm, Madison, WI 53706, USA and Univ Wisconsin, Dept Chem, Madison, WI 53706, USA","institution_ids":["https://openalex.org/I1304256225","https://openalex.org/I135310074"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":0.9546,"has_fulltext":false,"cited_by_count":23,"citation_normalized_percentile":{"value":0.73471387,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"227","issue":"1","first_page":"242","last_page":"245"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.5390999913215637,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.5390999913215637,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.07919999957084656,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.06340000033378601,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tyrosine-fluorescence","display_name":"tyrosine fluorescence","score":0.8730000257492065},{"id":"https://openalex.org/keywords/peptide-hydrolysis","display_name":"peptide hydrolysis","score":0.8920000195503235},{"id":"https://openalex.org/keywords/intrinsic-fluorescence","display_name":"intrinsic fluorescence","score":0.6209999918937683},{"id":"https://openalex.org/keywords/hiv-1-protease","display_name":"HIV-1 protease","score":0.828000009059906},{"id":"https://openalex.org/keywords/tyrosine-containing-peptides","display_name":"tyrosine-containing peptides","score":0.6349999904632568},{"id":"https://openalex.org/keywords/pepsin","display_name":"pepsin","score":0.8199999928474426},{"id":"https://openalex.org/keywords/trypsin","display_name":"trypsin","score":0.9980000257492065},{"id":"https://openalex.org/keywords/peptide-bond-hydrolysis","display_name":"peptide bond hydrolysis","score":0.5460000038146973}],"concepts":[{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.7666751146316528},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7496179342269897},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.7015487551689148},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.6704062223434448},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.6691591739654541},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.6584258079528809},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5052950978279114},{"id":"https://openalex.org/C31684184","wikidata":"https://www.wikidata.org/wiki/Q33293","display_name":"Peptide bond","level":3,"score":0.47916379570961},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.4638184905052185},{"id":"https://openalex.org/C2778471508","wikidata":"https://www.wikidata.org/wiki/Q425310","display_name":"Proteinase K","level":3,"score":0.42911115288734436},{"id":"https://openalex.org/C2778401398","wikidata":"https://www.wikidata.org/wiki/Q383836","display_name":"Chymotrypsin","level":4,"score":0.4184541404247284},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4056326746940613},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.40251457691192627},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.33823081851005554},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015683","descriptor_name":"Gene Products, gag","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016333","descriptor_name":"HIV Protease","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054301","descriptor_name":"gag Gene Products, Human Immunodeficiency Virus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1006/abio.1995.1276","doi":"https://doi.org/10.1006/abio.1995.1276","is_oa":false,"landing_page_url":"https://doi.org/10.1006/abio.1995.1276","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:7668386","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7668386","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8230110638","display_name":"SYNTHESIS AND ACTIVITY OF CYCLOSPORIN ANALOGS","funder_award_id":"R01AR032007","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"}],"funders":[{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2020681141","https://openalex.org/W2040598337","https://openalex.org/W2021024443","https://openalex.org/W2329854375","https://openalex.org/W2040861343","https://openalex.org/W2095235545","https://openalex.org/W2885002093","https://openalex.org/W2026460796","https://openalex.org/W2417989207","https://openalex.org/W2089993334"],"abstract_inverted_index":null,"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}