{"id":"https://openalex.org/W2014368495","doi":"https://doi.org/10.1016/j.jmb.2012.01.019","title":"Structure of the Ultra-High-Affinity Colicin E2 DNase\u2013Im2 Complex","display_name":"Structure of the Ultra-High-Affinity Colicin E2 DNase\u2013Im2 Complex","publication_year":2012,"publication_date":"2012-01-30","ids":{"openalex":"https://openalex.org/W2014368495","doi":"https://doi.org/10.1016/j.jmb.2012.01.019","mag":"2014368495","pmid":"https://pubmed.ncbi.nlm.nih.gov/22306467"},"language":"en","primary_location":{"id":"doi:10.1016/j.jmb.2012.01.019","doi":"https://doi.org/10.1016/j.jmb.2012.01.019","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jmb.2012.01.019","pdf_url":null,"source":{"id":"https://openalex.org/S46058472","display_name":"Journal of Molecular Biology","issn_l":"0022-2836","issn":["0022-2836","1089-8638"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Molecular Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5034073830","display_name":"J.A. Wojdyla","orcid":"https://orcid.org/0000-0002-8975-4137","observed_orcids":["https://orcid.org/0000-0002-8975-4137"]},"institutions":[{"id":"https://openalex.org/I52099693","display_name":"University of York","ror":"https://ror.org/04m01e293","country_code":"GB","type":"education","lineage":["https://openalex.org/I52099693"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Justyna Aleksandra Wojdyla","raw_affiliation_strings":["Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK","institution_ids":["https://openalex.org/I52099693"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031768744","display_name":"Sarel Jacob Fleishman","orcid":"https://orcid.org/0000-0003-3177-7560","observed_orcids":["https://orcid.org/0000-0003-3177-7560","https://orcid.org/0000-0002-6831-3770"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I53964585","display_name":"Weizmann Institute of Science","ror":"https://ror.org/0316ej306","country_code":"IL","type":"education","lineage":["https://openalex.org/I53964585"]}],"countries":["IL","US"],"is_corresponding":false,"raw_author_name":"Sarel J. Fleishman","raw_affiliation_strings":["Department of Biochemistry, University of Washington, Seattle, WA 98195, USA","Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel","institution_ids":["https://openalex.org/I53964585"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053192926","display_name":"David A. Baker","orcid":"https://orcid.org/0000-0001-7896-6217","observed_orcids":["https://orcid.org/0000-0001-7896-6217"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David Baker","raw_affiliation_strings":["Department of Biochemistry, University of Washington, Seattle, WA 98195, USA","Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5033107091","display_name":"Colin Kleanthous","orcid":"https://orcid.org/0000-0002-3273-0302","observed_orcids":["https://orcid.org/0000-0002-3273-0302"]},"institutions":[{"id":"https://openalex.org/I52099693","display_name":"University of York","ror":"https://ror.org/04m01e293","country_code":"GB","type":"education","lineage":["https://openalex.org/I52099693"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Colin Kleanthous","raw_affiliation_strings":["Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK","institution_ids":["https://openalex.org/I52099693"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5033107091"],"corresponding_institution_ids":["https://openalex.org/I52099693"],"apc_list":{"value":4730,"currency":"USD","value_usd":4730},"apc_paid":null,"fwci":3.3808,"has_fulltext":false,"cited_by_count":56,"citation_normalized_percentile":{"value":0.9335464,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"417","issue":"1-2","first_page":"79","last_page":"94"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5938000082969666,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5938000082969666,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.33149999380111694,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.022099999710917473,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/colicin-e2","display_name":"colicin E2","score":0.996999979019165},{"id":"https://openalex.org/keywords/protein-protein-complex","display_name":"protein-protein complex","score":0.7789999842643738},{"id":"https://openalex.org/keywords/high-affinity-binding","display_name":"high-affinity binding","score":0.7739999890327454},{"id":"https://openalex.org/keywords/binding-specificity","display_name":"binding specificity","score":0.6970000267028809},{"id":"https://openalex.org/keywords/protein-design","display_name":"protein design","score":0.3880000114440918},{"id":"https://openalex.org/keywords/frustration","display_name":"frustration","score":0.5690000057220459},{"id":"https://openalex.org/keywords/endonuclease-domain","display_name":"endonuclease domain","score":0.7300000190734863},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.5419999957084656}],"concepts":[{"id":"https://openalex.org/C168707992","wikidata":"https://www.wikidata.org/wiki/Q15439223","display_name":"Colicin","level":4,"score":0.8116351962089539},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5075246095657349},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.43572261929512024},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.4261387586593628},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4079464077949524},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.40488287806510925},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37508684396743774},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2799113392829895},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.2093733847141266},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12588831782341003}],"mesh":[{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003087","descriptor_name":"Colicins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003087","descriptor_name":"Colicins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014867","descriptor_name":"Water","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jmb.2012.01.019","doi":"https://doi.org/10.1016/j.jmb.2012.01.019","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jmb.2012.01.019","pdf_url":null,"source":{"id":"https://openalex.org/S46058472","display_name":"Journal of Molecular Biology","issn_l":"0022-2836","issn":["0022-2836","1089-8638"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Molecular Biology","raw_type":"journal-article"},{"id":"pmid:22306467","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22306467","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of molecular biology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8374543636","display_name":"Investigating E. coli cell envelope proteins and processes through colicin intoxication","funder_award_id":"BB/G020671/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G8987122162","display_name":null,"funder_award_id":"BB/G020671/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320320240","display_name":"Research Councils UK","ror":"https://ror.org/00dq2kk65"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":70,"referenced_works":["https://openalex.org/W1520730837","https://openalex.org/W1603332843","https://openalex.org/W1915490970","https://openalex.org/W1965650923","https://openalex.org/W1968394750","https://openalex.org/W1969361379","https://openalex.org/W1973721367","https://openalex.org/W1991633284","https://openalex.org/W1994001573","https://openalex.org/W1994101847","https://openalex.org/W1996351631","https://openalex.org/W2001641653","https://openalex.org/W2010143056","https://openalex.org/W2012667720","https://openalex.org/W2015183038","https://openalex.org/W2015305097","https://openalex.org/W2016240751","https://openalex.org/W2018249267","https://openalex.org/W2025895477","https://openalex.org/W2028302538","https://openalex.org/W2032675793","https://openalex.org/W2033785784","https://openalex.org/W2035503835","https://openalex.org/W2037511678","https://openalex.org/W2038840577","https://openalex.org/W2047696147","https://openalex.org/W2049275721","https://openalex.org/W2049966753","https://openalex.org/W2052466177","https://openalex.org/W2056034415","https://openalex.org/W2061922642","https://openalex.org/W2067653866","https://openalex.org/W2068190879","https://openalex.org/W2071704671","https://openalex.org/W2072186534","https://openalex.org/W2073484285","https://openalex.org/W2075770210","https://openalex.org/W2077578106","https://openalex.org/W2080223927","https://openalex.org/W2080428417","https://openalex.org/W2083702370","https://openalex.org/W2084568580","https://openalex.org/W2085458472","https://openalex.org/W2094392347","https://openalex.org/W2097493124","https://openalex.org/W2098702058","https://openalex.org/W2105822535","https://openalex.org/W2114597228","https://openalex.org/W2118517681","https://openalex.org/W2122339645","https://openalex.org/W2127995704","https://openalex.org/W2131747273","https://openalex.org/W2135832490","https://openalex.org/W2136640709","https://openalex.org/W2138059406","https://openalex.org/W2138905138","https://openalex.org/W2139605516","https://openalex.org/W2139833370","https://openalex.org/W2142972231","https://openalex.org/W2144081223","https://openalex.org/W2151635778","https://openalex.org/W2159247900","https://openalex.org/W2160690759","https://openalex.org/W2161743998","https://openalex.org/W2165054672","https://openalex.org/W2243956032","https://openalex.org/W2327128323","https://openalex.org/W3025428150","https://openalex.org/W4210359611","https://openalex.org/W4234210929"],"related_works":["https://openalex.org/W2080428417","https://openalex.org/W2165054672","https://openalex.org/W2010143056","https://openalex.org/W951009644","https://openalex.org/W2015183038","https://openalex.org/W1991633284","https://openalex.org/W2061922642","https://openalex.org/W1859414330","https://openalex.org/W2012667720","https://openalex.org/W2084568580"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":13},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}