{"id":"https://openalex.org/W2014367387","doi":"https://doi.org/10.1073/pnas.87.13.5203","title":"The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication.","display_name":"The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication.","publication_year":1990,"publication_date":"1990-07-01","ids":{"openalex":"https://openalex.org/W2014367387","doi":"https://doi.org/10.1073/pnas.87.13.5203","mag":"2014367387","pmid":"https://pubmed.ncbi.nlm.nih.gov/2367531","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/54290"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.87.13.5203","doi":"https://doi.org/10.1073/pnas.87.13.5203","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.87.13.5203","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/54290","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048322441","display_name":"Donald D. Wothe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D D Wothe","raw_affiliation_strings":["Department of Biochemistry, University of Washington, Seattle 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Washington, Seattle 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005668168","display_name":"Harry Charbonneau","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H Charbonneau","raw_affiliation_strings":["Department of Biochemistry, University of Washington, Seattle 98195","Department of Biochemistry SJ-70, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Washington, Seattle 98195","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Department of Biochemistry SJ-70, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113495308","display_name":"Bennett M. Shapiro","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"B M Shapiro","raw_affiliation_strings":["Department of Biochemistry, University of Washington, Seattle 98195","Department of Biochemistry SJ-70, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Washington, Seattle 98195","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Department of Biochemistry SJ-70, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":null,"apc_paid":null,"fwci":1.568,"has_fulltext":false,"cited_by_count":73,"citation_normalized_percentile":{"value":0.8267986,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"87","issue":"13","first_page":"5203","last_page":"5207"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.33719998598098755,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.33719998598098755,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.125,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.05860000103712082,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/creatine-kinase","display_name":"creatine kinase","score":0.7369999885559082},{"id":"https://openalex.org/keywords/sperm-flagella","display_name":"sperm flagella","score":0.7239999771118164},{"id":"https://openalex.org/keywords/sea-urchin-sperm","display_name":"sea urchin sperm","score":0.8379999995231628},{"id":"https://openalex.org/keywords/strongylocentrotus-purpuratus","display_name":"Strongylocentrotus purpuratus","score":0.9940000176429749},{"id":"https://openalex.org/keywords/gene-triplication","display_name":"gene triplication","score":0.8949999809265137},{"id":"https://openalex.org/keywords/axonemal-proteins","display_name":"axonemal proteins","score":0.7699999809265137},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.6669999957084656},{"id":"https://openalex.org/keywords/active-site-cysteine","display_name":"active site cysteine","score":0.8299999833106995},{"id":"https://openalex.org/keywords/arginine-kinase","display_name":"arginine kinase","score":0.7419999837875366},{"id":"https://openalex.org/keywords/phosphagen-kinases","display_name":"phosphagen kinases","score":0.8700000047683716},{"id":"https://openalex.org/keywords/energy-transport","display_name":"energy transport","score":0.44200000166893005}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7419795989990234},{"id":"https://openalex.org/C2777569064","wikidata":"https://www.wikidata.org/wiki/Q1936405","display_name":"Strongylocentrotus purpuratus","level":3,"score":0.7394317388534546},{"id":"https://openalex.org/C36880943","wikidata":"https://www.wikidata.org/wiki/Q409458","display_name":"Creatine kinase","level":2,"score":0.6214159727096558},{"id":"https://openalex.org/C2778626300","wikidata":"https://www.wikidata.org/wiki/Q1984607","display_name":"Phosphocreatine","level":3,"score":0.5853601694107056},{"id":"https://openalex.org/C2776277561","wikidata":"https://www.wikidata.org/wiki/Q4789582","display_name":"Arginine kinase","level":4,"score":0.5792855620384216},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.46014416217803955},{"id":"https://openalex.org/C2776908312","wikidata":"https://www.wikidata.org/wiki/Q83483","display_name":"Sea urchin","level":2,"score":0.44658640027046204},{"id":"https://openalex.org/C2780236403","wikidata":"https://www.wikidata.org/wiki/Q2355641","display_name":"Lactobacillus crispatus","level":4,"score":0.4175077974796295},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3589014708995819},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2492625117301941},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.21738797426223755},{"id":"https://openalex.org/C2777468819","wikidata":"https://www.wikidata.org/wiki/Q173670","display_name":"Arginine","level":3,"score":0.07139483094215393},{"id":"https://openalex.org/C2780206646","wikidata":"https://www.wikidata.org/wiki/Q1061596","display_name":"Lactobacillus","level":3,"score":0.0},{"id":"https://openalex.org/C2986317502","wikidata":"https://www.wikidata.org/wiki/Q570714","display_name":"Energy metabolism","level":2,"score":0.0},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003402","descriptor_name":"Creatine Kinase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003402","descriptor_name":"Creatine Kinase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003488","descriptor_name":"Cyanogen Bromide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005810","descriptor_name":"Multigene Family","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D010725","descriptor_name":"Phosphocreatine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012617","descriptor_name":"Sea Urchins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012689","descriptor_name":"Sequence Homology, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013082","descriptor_name":"Sperm Tail","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015345","descriptor_name":"Oligonucleotide Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.87.13.5203","doi":"https://doi.org/10.1073/pnas.87.13.5203","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.87.13.5203","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:54290","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/54290","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:2367531","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2367531","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Comparative Study"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:54290","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/54290","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1034740709","display_name":null,"funder_award_id":"CA 09437","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G1966490603","display_name":null,"funder_award_id":"GM 23910","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G4002980466","display_name":"MOLECULAR TRAINING PROGRAM IN CANCER RESEARCH - COMPETIN","funder_award_id":"T32CA009437","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G428350008","display_name":"MOLECULAR EVENTS IN FERTILIZATION","funder_award_id":"5r01gm023910-13","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W50754545","https://openalex.org/W176226702","https://openalex.org/W1499522144","https://openalex.org/W1513902446","https://openalex.org/W1518230311","https://openalex.org/W1542085789","https://openalex.org/W1559073010","https://openalex.org/W1569136570","https://openalex.org/W1571793851","https://openalex.org/W1575179156","https://openalex.org/W1610209323","https://openalex.org/W1967647423","https://openalex.org/W1991646097","https://openalex.org/W1998557764","https://openalex.org/W2007737728","https://openalex.org/W2014669590","https://openalex.org/W2018261167","https://openalex.org/W2018792069","https://openalex.org/W2024439805","https://openalex.org/W2029367636","https://openalex.org/W2043201277","https://openalex.org/W2044005753","https://openalex.org/W2045348184","https://openalex.org/W2048877959","https://openalex.org/W2069672597","https://openalex.org/W2086348243","https://openalex.org/W2101964511","https://openalex.org/W2138270253","https://openalex.org/W2159153637","https://openalex.org/W2168847939","https://openalex.org/W2178313645"],"related_works":["https://openalex.org/W1977258283","https://openalex.org/W1559073010","https://openalex.org/W2034067025","https://openalex.org/W77189024","https://openalex.org/W155739946","https://openalex.org/W2162254148","https://openalex.org/W2075121021","https://openalex.org/W7111882380","https://openalex.org/W2091358596","https://openalex.org/W1986098583"],"abstract_inverted_index":{"TCK,":[0],"the":[1,10,100,115,118],"creatine":[2,58,69,80,87,126],"kinase":[3,59,81,127,131],"(ATP:creatine":[4],"N-phosphotransferase)":[5],"from":[6,37,46],"sperm":[7],"flagella":[8],"of":[9,35,64,89,117,155],"sea":[11,48],"urchin":[12,49],"Strongylocentrotus":[13],"purpuratus,":[14],"is":[15,22,83],"a":[16,124,143,153],"Mr":[17],"145,000":[18],"axonemal":[19],"protein":[20],"that":[21,66],"employed":[23],"in":[24,128,132,138,147],"energy":[25],"transport.":[26],"Its":[27],"amino":[28,75],"acid":[29],"sequence":[30,65,105],"was":[31],"obtained":[32],"by":[33,42,62,73],"analysis":[34],"fragments":[36],"cyanogen":[38],"bromide":[39],"digestion":[40],"and":[41,71,76,92,95,134,149],"sequencing":[43],"cDNA":[44],"clones":[45],"two":[47],"testis":[50],"libraries.":[51],"TCK":[52,150],"contains":[53],"three":[54,97],"complete":[55],"but":[56],"nonidentical":[57],"segments":[60],"joined":[61],"regions":[63],"are":[67],"not":[68],"kinase-like":[70],"flanked":[72],"unique":[74,125],"carboxyl":[77],"termini.":[78],"Each":[79],"segment":[82],"homologous":[84],"to":[85],"vertebrate":[86],"kinases":[88,137],"both":[90,135],"muscle":[91],"brain":[93],"types,":[94],"all":[96],"repeats":[98,108],"contain":[99],"essential":[101],"active-site":[102],"cysteine.":[103],"The":[104,121],"differences":[106],"among":[107],"suggest":[109],"an":[110],"ancient":[111],"gene":[112],"triplication,":[113],"around":[114],"time":[116],"chordate-echinoderm":[119],"divergence.":[120],"echinoderm,":[122],"with":[123],"sperm,":[129],"arginine":[130],"eggs,":[133],"phosphagen":[136],"somatic":[139],"cells,":[140],"may":[141,151],"represent":[142],"preserved":[144],"branch":[145],"point":[146],"evolution,":[148],"be":[152],"relic":[154],"this":[156],"event.":[157]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}