{"id":"https://openalex.org/W2014345308","doi":"https://doi.org/10.1115/sbc2007-176682","title":"Quantitative Imaging of Nanoparticles and Intracellular Vesicle Trafficking Using Total Internal Reflection Fluorescent Microscopy (TIRFM)","display_name":"Quantitative Imaging of Nanoparticles and Intracellular Vesicle Trafficking Using Total Internal Reflection Fluorescent Microscopy (TIRFM)","publication_year":2007,"publication_date":"2007-06-20","ids":{"openalex":"https://openalex.org/W2014345308","doi":"https://doi.org/10.1115/sbc2007-176682","mag":"2014345308"},"language":"en","primary_location":{"id":"doi:10.1115/sbc2007-176682","doi":"https://doi.org/10.1115/sbc2007-176682","is_oa":false,"landing_page_url":"https://doi.org/10.1115/sbc2007-176682","pdf_url":null,"source":{"id":"https://openalex.org/S4306503435","display_name":"ASME Summer Bioengineering Conference (SBC)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ASME 2007 Summer Bioengineering Conference","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023694600","display_name":"Charles H. Margraves","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Charles H. Margraves","raw_affiliation_strings":["The University of Tennessee, Knoxville, TN","The University of Tennessee, Knoxville, TN#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN#TAB#","institution_ids":["https://openalex.org/I75027704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059118549","display_name":"Chang Kyoung Choi","orcid":"https://orcid.org/0000-0003-0061-4558","observed_orcids":["https://orcid.org/0000-0003-0061-4558"]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chang K. Choi","raw_affiliation_strings":["The University of Tennessee, Knoxville, TN","The University of Tennessee, Knoxville, TN#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN#TAB#","institution_ids":["https://openalex.org/I75027704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081163736","display_name":"Kenneth David Kihm","orcid":"https://orcid.org/0000-0001-5360-1764","observed_orcids":["https://orcid.org/0000-0001-5360-1764"]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth D. Kihm","raw_affiliation_strings":["The University of Tennessee, Knoxville, TN","The University of Tennessee, Knoxville, TN#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN#TAB#","institution_ids":["https://openalex.org/I75027704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010788062","display_name":"Anthony E. English","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anthony English","raw_affiliation_strings":["The University of Tennessee, Knoxville, TN","The University of Tennessee, Knoxville, TN#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN#TAB#","institution_ids":["https://openalex.org/I75027704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026658941","display_name":"Seong H. Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Seong H. Lee","raw_affiliation_strings":["The University of Tennessee, Knoxville, TN","The University of Tennessee, Knoxville, TN#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN#TAB#","institution_ids":["https://openalex.org/I75027704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012039134","display_name":"Maria Cekanova","orcid":"https://orcid.org/0000-0002-9651-1619","observed_orcids":["https://orcid.org/0000-0002-9651-1619"]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maria Cekanova","raw_affiliation_strings":["The University of Tennessee, Knoxville, TN","The University of Tennessee, Knoxville, TN#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN#TAB#","institution_ids":["https://openalex.org/I75027704"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009464125","display_name":"Seung Joon Baek","orcid":"https://orcid.org/0000-0001-7866-7778","observed_orcids":["https://orcid.org/0000-0001-7866-7778"]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Seung J. Baek","raw_affiliation_strings":["The University of Tennessee, Knoxville, TN","The University of Tennessee, Knoxville, TN#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"The University of Tennessee, Knoxville, TN#TAB#","institution_ids":["https://openalex.org/I75027704"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I75027704"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.13592233,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"583","last_page":"584"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/total-internal-reflection-fluorescence-microscopy","display_name":"total internal reflection fluorescence microscopy","score":0.9779999852180481},{"id":"https://openalex.org/keywords/nanoparticles","display_name":"nanoparticles","score":0.7979999780654907},{"id":"https://openalex.org/keywords/intracellular-vesicle-trafficking","display_name":"intracellular vesicle trafficking","score":0.9700000286102295},{"id":"https://openalex.org/keywords/quantitative-imaging","display_name":"quantitative imaging","score":0.8870000243186951},{"id":"https://openalex.org/keywords/dynamic-imaging","display_name":"dynamic imaging","score":0.6710000038146973},{"id":"https://openalex.org/keywords/green-fluorescent-protein","display_name":"green fluorescent protein","score":0.8029999732971191},{"id":"https://openalex.org/keywords/plasma-membrane","display_name":"plasma membrane","score":0.3310000002384186}],"concepts":[{"id":"https://openalex.org/C121381206","wikidata":"https://www.wikidata.org/wiki/Q902514","display_name":"Total internal reflection fluorescence microscope","level":3,"score":0.8445945978164673},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.7574818134307861},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.662977397441864},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.6511094570159912},{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.6499803066253662},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.5511903762817383},{"id":"https://openalex.org/C65682993","wikidata":"https://www.wikidata.org/wiki/Q1056451","display_name":"Reflection (computer programming)","level":2,"score":0.54816734790802},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.47084733843803406},{"id":"https://openalex.org/C2781294515","wikidata":"https://www.wikidata.org/wiki/Q2733470","display_name":"Stain","level":3,"score":0.4668857157230377},{"id":"https://openalex.org/C170395517","wikidata":"https://www.wikidata.org/wiki/Q234943","display_name":"Total internal reflection","level":2,"score":0.46239861845970154},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.443835586309433},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.42668479681015015},{"id":"https://openalex.org/C15526883","wikidata":"https://www.wikidata.org/wiki/Q6657126","display_name":"Live cell imaging","level":3,"score":0.4208938479423523},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.4036954343318939},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3977757394313812},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.39327919483184814},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31557697057724},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.28044432401657104},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.27698707580566406},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.21200266480445862},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.19824206829071045},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.16969481110572815},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.1367339789867401},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11909672617912292},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1115/sbc2007-176682","doi":"https://doi.org/10.1115/sbc2007-176682","is_oa":false,"landing_page_url":"https://doi.org/10.1115/sbc2007-176682","pdf_url":null,"source":{"id":"https://openalex.org/S4306503435","display_name":"ASME Summer Bioengineering Conference (SBC)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ASME 2007 Summer Bioengineering Conference","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1992950183","https://openalex.org/W2031320278","https://openalex.org/W2899050818","https://openalex.org/W2141744736","https://openalex.org/W4286853312","https://openalex.org/W7096474694","https://openalex.org/W2907780403","https://openalex.org/W2791374242","https://openalex.org/W2155653216","https://openalex.org/W2085687116"],"abstract_inverted_index":{"Total":[0],"internal":[1],"reflection":[2],"fluorescent":[3,71],"microscopy":[4],"(TIRFM)":[5],"is":[6,76],"a":[7,30,78],"relatively":[8],"well":[9],"known":[10],"tool":[11],"used":[12,27],"to":[13,34,60,81],"examine":[14,35],"the":[15,42,57,67],"near":[16],"wall":[17],"region":[18],"(approximately":[19],"1":[20],"\u03bcm).":[21],"For":[22],"years,":[23],"cellular":[24],"biologists":[25],"have":[26],"TIRFM":[28],"in":[29,48],"variety":[31],"of":[32,41,69],"experiments":[33],"multiple":[36],"cell":[37],"lines.":[38],"However,":[39],"much":[40],"research":[43],"has":[44],"been":[45],"somewhat":[46],"static":[47],"nature,":[49],"considering":[50],"only":[51],"initial":[52],"and":[53],"final":[54],"states.":[55],"With":[56],"increased":[58],"ability":[59],"stain":[61],"specific":[62],"organelles":[63],"within":[64],"cells":[65],"through":[66],"use":[68],"green":[70],"protein":[72],"(GFP),":[73],"dynamic":[74],"imaging":[75],"becoming":[77],"viable":[79],"solution":[80],"previously":[82],"difficult":[83],"problems.":[84]},"counts_by_year":[],"updated_date":"2026-10-06T10:28:23.643775","created_date":"2016-06-24T00:00:00"}