{"id":"https://openalex.org/W2013713227","doi":"https://doi.org/10.1016/j.febslet.2005.06.086","title":"Conformation\u2010driven and semiquinone\u2010gated proton\u2010pump mechanism in the NADH\u2010ubiquinone oxidoreductase (complex I)","display_name":"Conformation\u2010driven and semiquinone\u2010gated proton\u2010pump mechanism in the NADH\u2010ubiquinone oxidoreductase (complex I)","publication_year":2005,"publication_date":"2005-07-26","ids":{"openalex":"https://openalex.org/W2013713227","doi":"https://doi.org/10.1016/j.febslet.2005.06.086","mag":"2013713227","pmid":"https://pubmed.ncbi.nlm.nih.gov/16098512"},"language":"en","primary_location":{"id":"doi:10.1016/j.febslet.2005.06.086","doi":"https://doi.org/10.1016/j.febslet.2005.06.086","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.febslet.2005.06.086","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2005.06.086","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2005.06.086","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111519199","display_name":"Tomo\u0327ko Ohnishi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Tomoko Ohnishi","raw_affiliation_strings":["Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA. ohnishi@mail.med.upenn.edu","Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA. ohnishi@mail.med.upenn.edu","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, United States","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111541756","display_name":"John C. Salerno","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165799507","display_name":"Rensselaer Polytechnic Institute","ror":"https://ror.org/01rtyzb94","country_code":"US","type":"education","lineage":["https://openalex.org/I165799507"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John C. Salerno","raw_affiliation_strings":["Department of Biology, Rensselaer Polytechnic Institute, Troy, NY 12181, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Rensselaer Polytechnic Institute, Troy, NY 12181, United States","institution_ids":["https://openalex.org/I165799507"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111519199"],"corresponding_institution_ids":["https://openalex.org/I79576946"],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":4.894,"has_fulltext":true,"cited_by_count":96,"citation_normalized_percentile":{"value":0.96151388,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"579","issue":"21","first_page":"4555","last_page":"4561"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.7426999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.7426999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.08529999852180481,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13012","display_name":"Coenzyme Q10 studies and effects","score":0.04879999905824661,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/complex-i","display_name":"complex I","score":0.9649999737739563},{"id":"https://openalex.org/keywords/proton-pumping-mechanism","display_name":"proton pumping mechanism","score":0.6930000185966492},{"id":"https://openalex.org/keywords/semiquinone-radicals","display_name":"semiquinone","score":0.7979999780654907},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.7250000238418579},{"id":"https://openalex.org/keywords/spin-spin-coupling","display_name":"spin-spin coupling","score":0.6349999904632568},{"id":"https://openalex.org/keywords/proton-pumping","display_name":"proton translocation","score":0.7250000238418579},{"id":"https://openalex.org/keywords/mitochondrial-respiratory-chain","display_name":"mitochondrial respiratory chain","score":0.6460000276565552},{"id":"https://openalex.org/keywords/electron-transfer","display_name":"electron transfer","score":0.4129999876022339}],"concepts":[{"id":"https://openalex.org/C2776370595","wikidata":"https://www.wikidata.org/wiki/Q3955012","display_name":"Semiquinone","level":3,"score":0.9299339056015015},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6756007671356201},{"id":"https://openalex.org/C2780643102","wikidata":"https://www.wikidata.org/wiki/Q412382","display_name":"Quinone","level":2,"score":0.6292532682418823},{"id":"https://openalex.org/C2779268744","wikidata":"https://www.wikidata.org/wiki/Q407479","display_name":"Oxidoreductase","level":3,"score":0.5819159746170044},{"id":"https://openalex.org/C128892225","wikidata":"https://www.wikidata.org/wiki/Q412272","display_name":"Proton pump","level":4,"score":0.5463398694992065},{"id":"https://openalex.org/C204921945","wikidata":"https://www.wikidata.org/wiki/Q899689","display_name":"Side chain","level":3,"score":0.507089376449585},{"id":"https://openalex.org/C171357686","wikidata":"https://www.wikidata.org/wiki/Q910130","display_name":"Chemiosmosis","level":4,"score":0.4994845390319824},{"id":"https://openalex.org/C54516573","wikidata":"https://www.wikidata.org/wiki/Q2294","display_name":"Proton","level":2,"score":0.4973016083240509},{"id":"https://openalex.org/C24840226","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Electron transport chain","level":2,"score":0.4923049211502075},{"id":"https://openalex.org/C2777339483","wikidata":"https://www.wikidata.org/wiki/Q647203","display_name":"Conformational change","level":2,"score":0.47741734981536865},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4478241503238678},{"id":"https://openalex.org/C3020377403","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Respiratory chain","level":3,"score":0.444597989320755},{"id":"https://openalex.org/C90424259","wikidata":"https://www.wikidata.org/wiki/Q211026","display_name":"Electrochemical gradient","level":3,"score":0.44099417328834534},{"id":"https://openalex.org/C2909692557","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Electron Transport Complex I","level":4,"score":0.4294225871562958},{"id":"https://openalex.org/C123669783","wikidata":"https://www.wikidata.org/wiki/Q902898","display_name":"Electron transfer","level":2,"score":0.414481520652771},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.39232000708580017},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.3289985656738281},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.3255021274089813},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.30375581979751587},{"id":"https://openalex.org/C112243037","wikidata":"https://www.wikidata.org/wiki/Q22327117","display_name":"ATP synthase","level":3,"score":0.28672343492507935},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.23805701732635498},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1691845953464508},{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.15834152698516846},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14377933740615845},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.09780135750770569},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09153586626052856},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004579","descriptor_name":"Electron Transport","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016227","descriptor_name":"Benzoquinones","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017494","descriptor_name":"Proton Pumps","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D042967","descriptor_name":"Electron Transport Complex I","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.febslet.2005.06.086","doi":"https://doi.org/10.1016/j.febslet.2005.06.086","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.febslet.2005.06.086","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2005.06.086","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:16098512","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16098512","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.febslet.2005.06.086","doi":"https://doi.org/10.1016/j.febslet.2005.06.086","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.febslet.2005.06.086","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2005.06.086","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1957350756","display_name":null,"funder_award_id":"GM30736","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G3188499026","display_name":"Proton & electron transfer & energy coupling in site I","funder_award_id":"R01GM030736","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2013713227.pdf","grobid_xml":"https://content.openalex.org/works/W2013713227.grobid-xml"},"referenced_works_count":54,"referenced_works":["https://openalex.org/W1525364840","https://openalex.org/W1851154662","https://openalex.org/W1970204920","https://openalex.org/W1970548277","https://openalex.org/W1976798586","https://openalex.org/W1983487087","https://openalex.org/W1983925681","https://openalex.org/W1985811388","https://openalex.org/W1986499193","https://openalex.org/W1988312683","https://openalex.org/W1991280425","https://openalex.org/W2000389514","https://openalex.org/W2001063971","https://openalex.org/W2002825158","https://openalex.org/W2005969857","https://openalex.org/W2012003686","https://openalex.org/W2014960917","https://openalex.org/W2019356464","https://openalex.org/W2019881589","https://openalex.org/W2023719083","https://openalex.org/W2030716890","https://openalex.org/W2034704231","https://openalex.org/W2035446976","https://openalex.org/W2039386063","https://openalex.org/W2039957802","https://openalex.org/W2043823094","https://openalex.org/W2052611313","https://openalex.org/W2056106932","https://openalex.org/W2056753669","https://openalex.org/W2058182576","https://openalex.org/W2058330998","https://openalex.org/W2058423587","https://openalex.org/W2068754574","https://openalex.org/W2069693357","https://openalex.org/W2071904927","https://openalex.org/W2074362021","https://openalex.org/W2074519937","https://openalex.org/W2075529886","https://openalex.org/W2076017764","https://openalex.org/W2083037765","https://openalex.org/W2087524379","https://openalex.org/W2088767054","https://openalex.org/W2089558941","https://openalex.org/W2090383817","https://openalex.org/W2091696298","https://openalex.org/W2118479650","https://openalex.org/W2122013632","https://openalex.org/W2129871840","https://openalex.org/W2133205270","https://openalex.org/W2148208867","https://openalex.org/W2153487369","https://openalex.org/W2322997139","https://openalex.org/W3023019793","https://openalex.org/W4235128066"],"related_works":["https://openalex.org/W2087524379","https://openalex.org/W2058423587","https://openalex.org/W2063155454","https://openalex.org/W2032101013","https://openalex.org/W2161390673","https://openalex.org/W2028690975","https://openalex.org/W2810197078","https://openalex.org/W2001254662","https://openalex.org/W1972202832","https://openalex.org/W2065229855"],"abstract_inverted_index":{"A":[0,128],"novel":[1],"mechanism":[2,192],"for":[3,8,136,187],"proton/electron":[4],"transfer":[5],"is":[6,82,98,153],"proposed":[7],"NADH-quinone":[9],"oxidoreductase":[10],"(complex":[11],"I)":[12],"based":[13],"on":[14],"the":[15,22,35,55,62,79,89,94,106,111,119,125,132,139,145,156,160,165,173,178,191],"following":[16],"findings:":[17],"(1)":[18],"EPR":[19],"signals":[20],"of":[21,37,54,88,110,124,144,164,190,193],"protein-bound":[23],"fast-relaxing":[24],"semiquinone":[25,97],"anion":[26],"radicals":[27],"(abbreviated":[28],"as":[29],"Q(Nf)-)":[30],"are":[31,47],"observable":[32],"only":[33,59],"in":[34,196],"presence":[36],"proton-transmembrane":[38],"electrochemical":[39],"potential;":[40],"(2)":[41],"Iron-sulfur":[42],"cluster":[43],"N2":[44],"and":[45,50,69,115],"Q(Nf)-":[46],"directly":[48],"spin-coupled;":[49],"(3)":[51],"The":[52,168],"projection":[53],"interspin":[56],"vector":[57],"extends":[58],"5A":[60],"along":[61],"membrane":[63,114,146],"normal":[64],"[Yano,":[65],"T.,":[66],"Dunham,":[67],"W.R.":[68],"Ohnishi,":[70],"T.":[71],"(2005)":[72],"Biochemistry,":[73],"44,":[74],"1744-1754].":[75],"We":[76],"propose":[77],"that":[78],"proton":[80,194],"pump":[81],"operated":[83],"by":[84],"redox-driven":[85],"conformational":[86,129],"changes":[87],"quinone":[90,157],"binding":[91],"protein.":[92],"In":[93],"input":[95],"state,":[96],"reduced":[99],"to":[100,155,172,176],"quinol,":[101],"acquiring":[102],"two":[103],"protons":[104,133],"from":[105,118],"N":[107],"(matrix)":[108],"side":[109,123,143,163],"mitochondrial":[112],"inner":[113],"an":[116,151],"electron":[117,152],"low":[120],"potential":[121,162],"(NADH)":[122],"respiratory":[126],"chain.":[127],"change":[130],"brings":[131],"into":[134],"position":[135],"release":[137],"at":[138,159],"P":[140],"(inter-membrane":[141],"space)":[142],"via":[147],"a":[148,183],"proton-well.":[149],"Concomitantly,":[150],"donated":[154],"pool":[158],"high":[161],"coupling":[166],"site.":[167],"system":[169],"then":[170],"returns":[171],"original":[174],"state":[175],"repeat":[177],"cycle.":[179],"This":[180],"hypothesis":[181],"provides":[182],"useful":[184],"frame":[185],"work":[186],"further":[188],"investigation":[189],"translocation":[195],"complex":[197],"I.":[198]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":6}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}