{"id":"https://openalex.org/W2013166344","doi":"https://doi.org/10.1364/ao.35.002325","title":"Fluorescence-lifetime determination in tissues or other scattering media from measurement of excitation and emission kinetics","display_name":"Fluorescence-lifetime determination in tissues or other scattering media from measurement of excitation and emission kinetics","publication_year":1996,"publication_date":"1996-05-01","ids":{"openalex":"https://openalex.org/W2013166344","doi":"https://doi.org/10.1364/ao.35.002325","mag":"2013166344","pmid":"https://pubmed.ncbi.nlm.nih.gov/21085369"},"language":"en","primary_location":{"id":"doi:10.1364/ao.35.002325","doi":"https://doi.org/10.1364/ao.35.002325","is_oa":false,"landing_page_url":"https://doi.org/10.1364/ao.35.002325","pdf_url":null,"source":{"id":"https://openalex.org/S4210211917","display_name":"Applied Optics","issn_l":"0003-6935","issn":["0003-6935","1539-4522","1540-8981","1559-128X","2155-3165"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["anvur-scientific","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310315679","host_organization_name":"Optica Publishing Group","host_organization_lineage":["https://openalex.org/P4310315679"],"host_organization_lineage_names":["Optica Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Optics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076703471","display_name":"Christina L. Hutchinson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christina L. Hutchinson","raw_affiliation_strings":["The authors are with the School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The authors are with the School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022517334","display_name":"Tamara L. Troy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tamara L. Troy","raw_affiliation_strings":["The authors are with the School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The authors are with the School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025182463","display_name":"Eva Marie Sevick-Muraca","orcid":"https://orcid.org/0000-0002-8152-4847","observed_orcids":["https://orcid.org/0000-0002-8152-4847"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eva M. Sevick-Muraca","raw_affiliation_strings":["The authors are with the School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The authors are with the School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I219193219"],"apc_list":null,"apc_paid":null,"fwci":6.0695,"has_fulltext":false,"cited_by_count":47,"citation_normalized_percentile":{"value":0.96248376,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"35","issue":"13","first_page":"2325","last_page":"2325"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorescence-lifetime","display_name":"fluorescence lifetime","score":0.9369999766349792},{"id":"https://openalex.org/keywords/amplitude-demodulation","display_name":"amplitude demodulation","score":0.984000027179718},{"id":"https://openalex.org/keywords/turbid-media","display_name":"turbid media","score":0.6349999904632568},{"id":"https://openalex.org/keywords/tissue-optical-properties","display_name":"tissue optical properties","score":0.7009999752044678},{"id":"https://openalex.org/keywords/polystyrene-suspensions","display_name":"polystyrene suspensions","score":0.8309999704360962}],"concepts":[{"id":"https://openalex.org/C191486275","wikidata":"https://www.wikidata.org/wiki/Q210028","display_name":"Scattering","level":2,"score":0.6990074515342712},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.668380081653595},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6515997648239136},{"id":"https://openalex.org/C51141536","wikidata":"https://www.wikidata.org/wiki/Q838801","display_name":"Nanosecond","level":3,"score":0.6378498077392578},{"id":"https://openalex.org/C83581075","wikidata":"https://www.wikidata.org/wiki/Q1361503","display_name":"Excitation","level":2,"score":0.5746213793754578},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.5608965754508972},{"id":"https://openalex.org/C32891209","wikidata":"https://www.wikidata.org/wiki/Q483666","display_name":"Spectroscopy","level":2,"score":0.52084881067276},{"id":"https://openalex.org/C158711907","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectroscopy","level":3,"score":0.5150110721588135},{"id":"https://openalex.org/C120456961","wikidata":"https://www.wikidata.org/wiki/Q210028","display_name":"Light scattering","level":3,"score":0.462520956993103},{"id":"https://openalex.org/C133279788","wikidata":"https://www.wikidata.org/wiki/Q7804835","display_name":"Time-resolved spectroscopy","level":3,"score":0.43934255838394165},{"id":"https://openalex.org/C148869448","wikidata":"https://www.wikidata.org/wiki/Q184240","display_name":"Luminescence","level":2,"score":0.4312812387943268},{"id":"https://openalex.org/C6260449","wikidata":"https://www.wikidata.org/wiki/Q41364","display_name":"Wavelength","level":2,"score":0.4252217710018158},{"id":"https://openalex.org/C520434653","wikidata":"https://www.wikidata.org/wiki/Q38867","display_name":"Laser","level":2,"score":0.3697776794433594},{"id":"https://openalex.org/C184779094","wikidata":"https://www.wikidata.org/wiki/Q26383","display_name":"Atomic physics","level":1,"score":0.345944881439209},{"id":"https://openalex.org/C49040817","wikidata":"https://www.wikidata.org/wiki/Q193091","display_name":"Optoelectronics","level":1,"score":0.2866857945919037},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.21207818388938904},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1364/ao.35.002325","doi":"https://doi.org/10.1364/ao.35.002325","is_oa":false,"landing_page_url":"https://doi.org/10.1364/ao.35.002325","pdf_url":null,"source":{"id":"https://openalex.org/S4210211917","display_name":"Applied Optics","issn_l":"0003-6935","issn":["0003-6935","1539-4522","1540-8981","1559-128X","2155-3165"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["anvur-scientific","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310315679","host_organization_name":"Optica Publishing Group","host_organization_lineage":["https://openalex.org/P4310315679"],"host_organization_lineage_names":["Optica Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Optics","raw_type":"journal-article"},{"id":"pmid:21085369","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21085369","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied optics","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1966411342","https://openalex.org/W1977691661","https://openalex.org/W1979781686","https://openalex.org/W2006436439","https://openalex.org/W2020444919","https://openalex.org/W2034028838","https://openalex.org/W2035054183","https://openalex.org/W2054794026","https://openalex.org/W2057540366","https://openalex.org/W2063674368","https://openalex.org/W2088995024","https://openalex.org/W2090088271","https://openalex.org/W2122906753","https://openalex.org/W2139701685"],"related_works":["https://openalex.org/W2061576920","https://openalex.org/W2032094364","https://openalex.org/W2280860830","https://openalex.org/W2337262635","https://openalex.org/W2087764828","https://openalex.org/W2322062067","https://openalex.org/W1487982431","https://openalex.org/W2006743459","https://openalex.org/W2032975852","https://openalex.org/W2053417620"],"abstract_inverted_index":{"Measurements":[0],"of":[1,19,35,49],"nanosecond":[2],"and":[3,15,37,43,53,72,94],"subnanosecond":[4],"fluorescence":[5],"lifetimes":[6,50],"are":[7],"restricted":[8],"to":[9],"dilute,":[10],"nonscattering":[11],"systems":[12],"since":[13],"excitation":[14,42],"emission":[16,44],"photon":[17],"times":[18],"flight":[20],"significantly":[21],"affect":[22],"measured":[23],"fluorescent":[24],"decay":[25],"kinetics.":[26],"We":[27,57],"provide":[28],"the":[29],"theoretical":[30],"rationale":[31],"for":[32,46,89],"frequency-domain":[33],"measurements":[34],"phase-shift":[36],"amplitude":[38],"demodulation":[39],"made":[40],"at":[41],"wavelengths":[45],"direct":[47],"determination":[48],"in":[51,75,92],"tissues":[52,93],"other":[54,95],"scattering":[55,80,96],"media.":[56,97],"confirm":[58],"our":[59],"analytical":[60],"expressions":[61],"using":[62],"standard":[63],"laser":[64],"dyes":[65],"such":[66],"as":[67,82],"3,3'-diethylthiatricarbocyanine":[68],"iodide,":[69],"IR-":[70,73],"125,":[71],"140":[74],"polystyrene":[76],"suspensions":[77],"with":[78],"similar":[79],"properties":[81],"tissues.":[83],"Our":[84],"results":[85],"have":[86],"significant":[87],"implication":[88],"lifetime-based":[90],"spectroscopy":[91]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}