{"id":"https://openalex.org/W2013139845","doi":"https://doi.org/10.1271/bbb.61.875","title":"Cloning and Sequencing of a cDNA Encoding \u03b1-Glucosidase from Sugar Beet","display_name":"Cloning and Sequencing of a cDNA Encoding \u03b1-Glucosidase from Sugar Beet","publication_year":1997,"publication_date":"1997-01-01","ids":{"openalex":"https://openalex.org/W2013139845","doi":"https://doi.org/10.1271/bbb.61.875","mag":"2013139845","pmid":"https://pubmed.ncbi.nlm.nih.gov/9178565"},"language":"en","primary_location":{"id":"doi:10.1271/bbb.61.875","doi":"https://doi.org/10.1271/bbb.61.875","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.61.875","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000409773","display_name":"Hirokazu Matsui","orcid":"https://orcid.org/0000-0002-5078-3387","observed_orcids":["https://orcid.org/0000-0002-5078-3387"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hirokazu Matsui","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005725596","display_name":"Shunsuke Iwanami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shunsuke Iwanami","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101855938","display_name":"Hiroyuki Ito","orcid":"https://orcid.org/0000-0001-7634-1424","observed_orcids":["https://orcid.org/0000-0001-7634-1424"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroyuki Ito","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006117348","display_name":"Haruhide Mori","orcid":"https://orcid.org/0000-0001-7346-9131","observed_orcids":["https://orcid.org/0000-0001-7346-9131"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Haruhide Mori","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110150398","display_name":"Mamoru Honma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mamoru Honma","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060068188","display_name":"Seiya Chiba","orcid":"https://orcid.org/0009-0008-4852-2541","observed_orcids":["https://orcid.org/0009-0008-4852-2541"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Seiya Chiba","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060, Japan","institution_ids":["https://openalex.org/I205349734"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5000409773"],"corresponding_institution_ids":["https://openalex.org/I205349734"],"apc_list":{"value":3272,"currency":"USD","value_usd":3272},"apc_paid":null,"fwci":2.7005,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.88665448,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"61","issue":"5","first_page":"875","last_page":"880"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.47269999980926514,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.47269999980926514,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.1289999932050705,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.05869999900460243,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-glucosidase","display_name":"alpha-glucosidase","score":0.9869999885559082},{"id":"https://openalex.org/keywords/sugar-beet","display_name":"sugar beet","score":0.9990000128746033},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.8270000219345093},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.7369999885559082},{"id":"https://openalex.org/keywords/alpha-amylase-family","display_name":"alpha-amylase family","score":0.8840000033378601},{"id":"https://openalex.org/keywords/active-site","display_name":"active site","score":0.4429999887943268},{"id":"https://openalex.org/keywords/proteolytic-peptides","display_name":"proteolytic peptides","score":0.781000018119812},{"id":"https://openalex.org/keywords/sequence-homology","display_name":"sequence homology","score":0.8560000061988831},{"id":"https://openalex.org/keywords/suspension-cultured-cells","display_name":"suspension-cultured cells","score":0.8299999833106995}],"concepts":[{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.8448940515518188},{"id":"https://openalex.org/C47289529","wikidata":"https://www.wikidata.org/wiki/Q908550","display_name":"Open reading frame","level":4,"score":0.7080531120300293},{"id":"https://openalex.org/C92825506","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Protein primary structure","level":4,"score":0.5766651630401611},{"id":"https://openalex.org/C2776936025","wikidata":"https://www.wikidata.org/wiki/Q151964","display_name":"Sugar beet","level":2,"score":0.576653242111206},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.5712899565696716},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5546125173568726},{"id":"https://openalex.org/C165525559","wikidata":"https://www.wikidata.org/wiki/Q224180","display_name":"Homology (biology)","level":3,"score":0.543308436870575},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5005431175231934},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.49439191818237305},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.49072110652923584},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4503244459629059},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.42434513568878174},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.27728068828582764},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000520","descriptor_name":"alpha-Glucosidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005815","descriptor_name":"Genetic Code","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014675","descriptor_name":"Vegetables","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D014675","descriptor_name":"Vegetables","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D014675","descriptor_name":"Vegetables","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015345","descriptor_name":"Oligonucleotide Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017124","descriptor_name":"Conserved Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1271/bbb.61.875","doi":"https://doi.org/10.1271/bbb.61.875","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.61.875","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},{"id":"pmid:9178565","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9178565","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, biotechnology, and biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":2,"referenced_works":["https://openalex.org/W2144634347","https://openalex.org/W4253565803"],"related_works":["https://openalex.org/W1544068576","https://openalex.org/W2423165620","https://openalex.org/W2007102703","https://openalex.org/W1976595134","https://openalex.org/W2031062189","https://openalex.org/W1874975980","https://openalex.org/W2045844958","https://openalex.org/W7064597525","https://openalex.org/W2402153268","https://openalex.org/W2081401851"],"abstract_inverted_index":{"A":[0],"cDNA":[1,68],"encoding":[2,28],"sugar":[3,83],"beet":[4,84],"alpha-glucosidase":[5],"was":[6,108],"cloned":[7],"from":[8,12,63],"a":[9,29,37],"library":[10],"constructed":[11],"mRNA":[13],"of":[14,31,40,46,56,66,72,82,96],"suspension-cultured":[15],"cells.":[16],"The":[17,58,86,105],"cDNA,":[18],"3056":[19],"bp":[20],"in":[21,52,93,110],"length,":[22],"had":[23],"an":[24],"open":[25],"reading":[26],"frame":[27],"polypeptide":[30],"913":[32],"amino":[33,60],"acid":[34,61],"residues":[35],"with":[36],"molecular":[38],"mass":[39],"102,078":[41],"Da,":[42],"included":[43],"only":[44],"one":[45],"four":[47],"regions":[48],"which":[49],"were":[50],"conserved":[51],"the":[53,64,67,70,73,77,94],"alpha-amylase":[54],"family":[55],"enzymes.":[57],"deduced":[59],"sequence":[62],"analysis":[65],"contained":[69],"sequences":[71],"proteolysis":[74],"peptides":[75],"and":[76],"active":[78],"site":[79],"region":[80],"peptide":[81],"alpha-glucosidase.":[85,112],"primary":[87],"structure":[88],"indicated":[89],"relatively":[90],"high":[91],"homology":[92,107],"range":[95],"28.2":[97],"to":[98,100],"54.3%":[99],"those":[101],"for":[102],"other":[103],"alpha-glucosidases.":[104],"highest":[106],"found":[109],"barley":[111]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}