{"id":"https://openalex.org/W2013066045","doi":"https://doi.org/10.1186/s12896-015-0117-0","title":"Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum","display_name":"Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum","publication_year":2015,"publication_date":"2015-02-07","ids":{"openalex":"https://openalex.org/W2013066045","doi":"https://doi.org/10.1186/s12896-015-0117-0","mag":"2013066045","pmid":"https://pubmed.ncbi.nlm.nih.gov/25887674","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4333176"},"language":"en","primary_location":{"id":"doi:10.1186/s12896-015-0117-0","doi":"https://doi.org/10.1186/s12896-015-0117-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12896-015-0117-0","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/s12896-015-0117-0","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/s12896-015-0117-0","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023350983","display_name":"Narendra Kumar Tuteja","orcid":"https://orcid.org/0000-0002-6157-0264","observed_orcids":["https://orcid.org/0000-0002-6157-0264"]},"institutions":[{"id":"https://openalex.org/I4210142124","display_name":"International Centre for Genetic Engineering and Biotechnology","ror":"https://ror.org/03j4rrt43","country_code":"IN","type":"facility","lineage":["https://openalex.org/I4210142124","https://openalex.org/I91080651"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Narendra Tuteja","raw_affiliation_strings":["Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India. narendra@icgeb.res.in"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India. narendra@icgeb.res.in","institution_ids":["https://openalex.org/I4210142124"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048159223","display_name":"Panchanand Mishra","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210118921","display_name":"Institute of Life Sciences","ror":"https://ror.org/02927dx12","country_code":"IN","type":"education","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210118921","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Panchanand Mishra","raw_affiliation_strings":["Stress Biology Laboratory, Gene Function and Regulation, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India. panchanand51@gmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stress Biology Laboratory, Gene Function and Regulation, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India. panchanand51@gmail.com","institution_ids":["https://openalex.org/I4210118921"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070126588","display_name":"Sandep Yadav","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142124","display_name":"International Centre for Genetic Engineering and Biotechnology","ror":"https://ror.org/03j4rrt43","country_code":"IN","type":"facility","lineage":["https://openalex.org/I4210142124","https://openalex.org/I91080651"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sandep Yadav","raw_affiliation_strings":["Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India. sandeep@icgeb.res.in"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India. sandeep@icgeb.res.in","institution_ids":["https://openalex.org/I4210142124"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074964602","display_name":"Marjan M. Tajrishi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142124","display_name":"International Centre for Genetic Engineering and Biotechnology","ror":"https://ror.org/03j4rrt43","country_code":"IN","type":"facility","lineage":["https://openalex.org/I4210142124","https://openalex.org/I91080651"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Marjan Tajrishi","raw_affiliation_strings":["Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India. marjant1359@yahoo.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India. marjant1359@yahoo.com","institution_ids":["https://openalex.org/I4210142124"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Sudhir Baral","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210118921","display_name":"Institute of Life Sciences","ror":"https://ror.org/02927dx12","country_code":"IN","type":"education","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210118921","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sudhir Baral","raw_affiliation_strings":["Stress Biology Laboratory, Gene Function and Regulation, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India. sudhir.baral88@gmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stress Biology Laboratory, Gene Function and Regulation, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India. sudhir.baral88@gmail.com","institution_ids":["https://openalex.org/I4210118921"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110139720","display_name":"Surendra Chandra Sabat","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210118921","display_name":"Institute of Life Sciences","ror":"https://ror.org/02927dx12","country_code":"IN","type":"education","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210118921","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Surendra Chandra Sabat","raw_affiliation_strings":["Stress Biology Laboratory, Gene Function and Regulation, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India. surendrachandra@gmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stress Biology Laboratory, Gene Function and Regulation, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India. surendrachandra@gmail.com","institution_ids":["https://openalex.org/I4210118921"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5023350983","https://openalex.org/A5110139720"],"corresponding_institution_ids":["https://openalex.org/I4210118921","https://openalex.org/I4210142124"],"apc_list":{"value":2990,"currency":"USD","value_usd":2990},"apc_paid":{"value":2990,"currency":"USD","value_usd":2990},"fwci":2.0487,"has_fulltext":true,"cited_by_count":22,"citation_normalized_percentile":{"value":0.83193408,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"15","issue":"1","first_page":"3","last_page":"3"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.7324000000953674,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.7324000000953674,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.09799999743700027,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.05350000038743019,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cu-zn-superoxide-dismutase","display_name":"Cu/Zn superoxide dismutase","score":0.9810000061988831},{"id":"https://openalex.org/keywords/pisum-sativum","display_name":"Pisum sativum","score":0.9919999837875366},{"id":"https://openalex.org/keywords/pea","display_name":"pea","score":0.8920000195503235},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9079999923706055},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.8529999852180481},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.628000020980835},{"id":"https://openalex.org/keywords/enzyme-stability","display_name":"enzyme stability","score":0.7519999742507935},{"id":"https://openalex.org/keywords/antioxidant-activity","display_name":"antioxidant activity","score":0.5329999923706055},{"id":"https://openalex.org/keywords/peroxidase-activity","display_name":"peroxidase activity","score":0.953000009059906},{"id":"https://openalex.org/keywords/culture-condition-optimization","display_name":"culture condition optimization","score":0.48399999737739563}],"concepts":[{"id":"https://openalex.org/C2775838275","wikidata":"https://www.wikidata.org/wiki/Q410776","display_name":"Superoxide dismutase","level":3,"score":0.8718863725662231},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7621287107467651},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6820147037506104},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6623493432998657},{"id":"https://openalex.org/C2779535332","wikidata":"https://www.wikidata.org/wiki/Q144795","display_name":"Pisum","level":2,"score":0.6330310702323914},{"id":"https://openalex.org/C2780795997","wikidata":"https://www.wikidata.org/wiki/Q413283","display_name":"Superoxide","level":3,"score":0.580070972442627},{"id":"https://openalex.org/C2775997218","wikidata":"https://www.wikidata.org/wiki/Q1166350","display_name":"Sativum","level":2,"score":0.5408353209495544},{"id":"https://openalex.org/C162008176","wikidata":"https://www.wikidata.org/wiki/Q419305","display_name":"Peroxidase","level":3,"score":0.4913085997104645},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.48648473620414734},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.4729998707771301},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.07884061336517334},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06467694044113159}],"mesh":[{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013482","descriptor_name":"Superoxide Dismutase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013482","descriptor_name":"Superoxide Dismutase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013482","descriptor_name":"Superoxide Dismutase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013862","descriptor_name":"Thiogalactosides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018532","descriptor_name":"Pisum sativum","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D018532","descriptor_name":"Pisum sativum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018532","descriptor_name":"Pisum sativum","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1186/s12896-015-0117-0","doi":"https://doi.org/10.1186/s12896-015-0117-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12896-015-0117-0","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/s12896-015-0117-0","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},{"id":"pmid:25887674","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25887674","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4333176","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4333176/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/s12896-015-0117-0","doi":"https://doi.org/10.1186/s12896-015-0117-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12896-015-0117-0","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/s12896-015-0117-0","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.40450000762939453,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320717","display_name":"Department of Biotechnology, Ministry of Science and Technology, India","ror":"https://ror.org/03tjsyq23"},{"id":"https://openalex.org/F4320320719","display_name":"Department of Science and Technology, Ministry of Science and Technology, India","ror":"https://ror.org/0101xrq71"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2013066045.pdf","grobid_xml":"https://content.openalex.org/works/W2013066045.grobid-xml"},"referenced_works_count":55,"referenced_works":["https://openalex.org/W1521735421","https://openalex.org/W1836596282","https://openalex.org/W1888952383","https://openalex.org/W1921654606","https://openalex.org/W1965208511","https://openalex.org/W1970732127","https://openalex.org/W1975695493","https://openalex.org/W1981625522","https://openalex.org/W1984732586","https://openalex.org/W1986438501","https://openalex.org/W1993096570","https://openalex.org/W1997453918","https://openalex.org/W1999300802","https://openalex.org/W2000765714","https://openalex.org/W2007434696","https://openalex.org/W2008693724","https://openalex.org/W2021535684","https://openalex.org/W2031097266","https://openalex.org/W2032463138","https://openalex.org/W2034046670","https://openalex.org/W2037315437","https://openalex.org/W2037895677","https://openalex.org/W2040592121","https://openalex.org/W2059156656","https://openalex.org/W2063680592","https://openalex.org/W2067888080","https://openalex.org/W2070534373","https://openalex.org/W2085528344","https://openalex.org/W2088659768","https://openalex.org/W2088715767","https://openalex.org/W2088866343","https://openalex.org/W2101422765","https://openalex.org/W2108364179","https://openalex.org/W2110738263","https://openalex.org/W2116067127","https://openalex.org/W2116507604","https://openalex.org/W2118200955","https://openalex.org/W2119006550","https://openalex.org/W2121117469","https://openalex.org/W2121608468","https://openalex.org/W2126386203","https://openalex.org/W2128635872","https://openalex.org/W2130122826","https://openalex.org/W2137015675","https://openalex.org/W2137199843","https://openalex.org/W2138454298","https://openalex.org/W2152301430","https://openalex.org/W2154411853","https://openalex.org/W2158649428","https://openalex.org/W2161733672","https://openalex.org/W2163553212","https://openalex.org/W2170913004","https://openalex.org/W2245457119","https://openalex.org/W4230151378","https://openalex.org/W4231524173"],"related_works":["https://openalex.org/W2061653279","https://openalex.org/W2076912551","https://openalex.org/W2969036062","https://openalex.org/W2051272283","https://openalex.org/W1987971347","https://openalex.org/W2168068581","https://openalex.org/W2055627116","https://openalex.org/W2475340765","https://openalex.org/W2017399386","https://openalex.org/W2002485374"],"abstract_inverted_index":{"BACKGROUND:":[0],"CuZn-Superoxide":[1],"dismutase":[2,181,264,285],"(SOD)":[3],"is":[4,55],"a":[5,258],"unique":[6],"enzyme,":[7],"which":[8],"can":[9,275],"catalyzes":[10],"the":[11,67,75,78,91,107,115,146,159,184,207,211,255,279,299],"dismutation":[12],"of":[13,45,61,66,77,90,94,114,136,145,155,214,217,237,257,282,301],"inevitable":[14],"metabolic":[15],"product":[16],"i.e.;":[17],"superoxide":[18,180],"anion":[19],"into":[20],"molecular":[21],"oxygen":[22],"and":[23,52,58,64,84,106,125,141,158,183,202,231,235,245,261,286,295,308],"hydrogen":[24],"peroxide.":[25],"The":[26,42,167,199,219,273],"enzyme":[27,79,220],"has":[28,289],"gained":[29],"wide":[30,290],"interest":[31],"in":[32,48,80,117,132,265,269,292],"pharmaceutical":[33,293],"industries":[34,294],"due":[35],"to":[36,73,174,194,223],"its":[37,49,118,266],"highly":[38,56,259],"acclaimed":[39],"antioxidative":[40],"properties.":[41],"recombinant":[43,148,168,267],"expression":[44,89],"this":[46,215,302],"protein":[47,116,127,149,208,303],"enzymatically":[50],"active":[51,260,283],"stable":[53,226,262],"form":[54,268],"desired":[57],"hence":[59],"optimization":[60],"culture":[62,156],"conditions":[63],"characterization":[65,204],"related":[68],"biochemical":[69,203],"properties":[70],"are":[71],"essential":[72],"explore":[74],"significance":[76],"physiological,":[81],"therapeutic,":[82],"structural":[83],"transgenic":[85],"research.":[86],"RESULTS:":[87],"High-level":[88],"chloroplastic":[92,170],"isoform":[93],"Pisum":[95],"sativum":[96],"CuZn-SOD":[97],"was":[98,109,150,162,172,187,221],"achieved":[99],"at":[100],"18\u00b0C,":[101],"upon":[102,240],"isopropyl":[103],"\u03b2-D-1-thiogalactopyranoside":[104],"induction":[105],"process":[108],"optimized":[110],"for":[111,254,278,297],"maximum":[112],"recovery":[113],"soluble":[119],"(enzymatically":[120],"active)":[121],"form.":[122],"Both":[123],"crude":[124],"purified":[126,147],"fractions":[128],"display":[129],"significant":[130],"increase":[131],"activity":[133,182,186,239],"following":[134],"supplementation":[135],"defined":[137],"concentration":[138],"Cu":[139],"(CuSO4)":[140],"Zn":[142],"(ZnSO4).":[143],"Yield":[144],"~":[151,163],"4":[152],"mg":[153],"L(-1)":[154],"volume":[157],"bacterial":[160],"biomass":[161],"4.5":[164],"g":[165],"L(-1).":[166],"pea":[169],"SOD":[171,238],"found":[173,222],"possess":[175],"nearly":[176],"6":[177],"fold":[178,190],"higher":[179,191],"peroxidase":[185],"also":[188,296],"5":[189],"as":[192,227],"compared":[193],"commercially":[195],"available":[196],"CuZn-superoxide":[197,263,284],"dismutase.":[198],"computational,":[200],"spectroscopic":[201],"reveals":[205],"that":[206],"harbors":[209],"all":[210],"characteristics":[212],"features":[213],"class":[216],"enzyme.":[218],"be":[224,276],"exceptionally":[225],"evident":[228],"from":[229],"pH":[230],"temperature":[232],"incubation":[233],"studies":[234],"maintenance":[236],"prolonged":[241],"storage.":[242],"CONCLUSIONS:":[243],"Overexpression":[244],"purification":[246],"strategy":[247,274],"presented":[248],"here":[249],"describes":[250],"an":[251],"efficient":[252],"protocol":[253],"production":[256],"E.":[270],"coli":[271],"system.":[272],"utilized":[277],"large-scale":[280],"preparation":[281],"thus":[287],"it":[288],"application":[291],"elucidating":[298],"potential":[300],"endowed":[304],"with":[305],"exceptional":[306],"stability":[307],"activity.":[309]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}