{"id":"https://openalex.org/W2012993585","doi":"https://doi.org/10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","title":"Characterization of the guanidinobenzoatase in the epididymal fluids of the mouse","display_name":"Characterization of the guanidinobenzoatase in the epididymal fluids of the mouse","publication_year":1997,"publication_date":"1997-06-01","ids":{"openalex":"https://openalex.org/W2012993585","doi":"https://doi.org/10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","mag":"2012993585","pmid":"https://pubmed.ncbi.nlm.nih.gov/9136123"},"language":"en","primary_location":{"id":"doi:10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","doi":"https://doi.org/10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","pdf_url":null,"source":{"id":"https://openalex.org/S125242520","display_name":"Molecular Reproduction and Development","issn_l":"1040-452X","issn":["1040-452X","1098-2795"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Reproduction and Development","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000440089","display_name":"Zachary L Barksdale","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17301866","display_name":"University of Alabama","ror":"https://ror.org/03xrrjk67","country_code":"US","type":"education","lineage":["https://openalex.org/I17301866"]},{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zachary L. Barksdale","raw_affiliation_strings":["Department of Biology, University of Alabama, Birmingham, Alabama","Department of Biology, University of Alabama, Birmingham 35294, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Alabama, Birmingham, Alabama","institution_ids":["https://openalex.org/I32389192"]},{"raw_affiliation_string":"Department of Biology, University of Alabama, Birmingham 35294, USA","institution_ids":["https://openalex.org/I17301866","https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106134906","display_name":"Scott E. Caldwell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott E. Caldwell","raw_affiliation_strings":["Department of Biology, University of Alabama, Birmingham, Alabama"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Alabama, Birmingham, Alabama","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080198273","display_name":"David J. Aarons","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181982445","display_name":"Talladega College","ror":"https://ror.org/01dswgm35","country_code":"US","type":"education","lineage":["https://openalex.org/I181982445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David J. Aarons","raw_affiliation_strings":["Department of Biology, Talladega College, Talladega, Alabama"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Talladega College, Talladega, Alabama","institution_ids":["https://openalex.org/I181982445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018588042","display_name":"Shondria B. Young","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181982445","display_name":"Talladega College","ror":"https://ror.org/01dswgm35","country_code":"US","type":"education","lineage":["https://openalex.org/I181982445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shondria B. Young","raw_affiliation_strings":["Department of Biology, Talladega College, Talladega, Alabama"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Talladega College, Talladega, Alabama","institution_ids":["https://openalex.org/I181982445"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084362155","display_name":"Gary R. Poirier","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17301866","display_name":"University of Alabama","ror":"https://ror.org/03xrrjk67","country_code":"US","type":"education","lineage":["https://openalex.org/I17301866"]},{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gary R. Poirier","raw_affiliation_strings":["Department of Biology, University of Alabama, Birmingham, Alabama","Dept. of Biology, University of Alabama, University Station, Birmingham, AL 35294"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Alabama, Birmingham, Alabama","institution_ids":["https://openalex.org/I32389192"]},{"raw_affiliation_string":"Dept. of Biology, University of Alabama, University Station, Birmingham, AL 35294","institution_ids":["https://openalex.org/I17301866","https://openalex.org/I32389192"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5084362155"],"corresponding_institution_ids":["https://openalex.org/I17301866","https://openalex.org/I32389192"],"apc_list":{"value":4260,"currency":"USD","value_usd":4260},"apc_paid":null,"fwci":1.0085,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.76840773,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"47","issue":"2","first_page":"204","last_page":"209"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.98580002784729,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.98580002784729,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/epididymal-fluid","display_name":"epididymal fluid","score":0.9739999771118164},{"id":"https://openalex.org/keywords/male-genital-tract","display_name":"male genital tract","score":0.5090000033378601},{"id":"https://openalex.org/keywords/sperm-capacitation","display_name":"sperm capacitation","score":0.4909999966621399},{"id":"https://openalex.org/keywords/zona-pellucida","display_name":"zona pellucida","score":0.777999997138977},{"id":"https://openalex.org/keywords/zp3","display_name":"ZP3","score":0.9369999766349792},{"id":"https://openalex.org/keywords/serine-protease-inhibitors","display_name":"serine protease inhibitors","score":0.796999990940094},{"id":"https://openalex.org/keywords/trypsin-inhibitor","display_name":"trypsin inhibitor","score":0.7850000262260437},{"id":"https://openalex.org/keywords/sperm-binding","display_name":"sperm binding","score":0.45500001311302185},{"id":"https://openalex.org/keywords/gamete-recognition","display_name":"gamete recognition","score":0.9419999718666077},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.6729999780654907}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8120303153991699},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6743154525756836},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5491833090782166},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.540086567401886},{"id":"https://openalex.org/C2909000736","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine Proteinase Inhibitors","level":5,"score":0.5265013575553894},{"id":"https://openalex.org/C2777658833","wikidata":"https://www.wikidata.org/wiki/Q408437","display_name":"EGTA","level":3,"score":0.5157737731933594},{"id":"https://openalex.org/C2776803348","wikidata":"https://www.wikidata.org/wiki/Q211050","display_name":"Gamete","level":3,"score":0.5044945478439331},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.4954298734664917},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.480175644159317},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.46935755014419556},{"id":"https://openalex.org/C2777447553","wikidata":"https://www.wikidata.org/wiki/Q413934","display_name":"Acrosin","level":4,"score":0.4688946604728699},{"id":"https://openalex.org/C2781254305","wikidata":"https://www.wikidata.org/wiki/Q1973610","display_name":"Epididymis","level":3,"score":0.4422791302204132},{"id":"https://openalex.org/C2780865716","wikidata":"https://www.wikidata.org/wiki/Q411575","display_name":"PMSF","level":3,"score":0.41864028573036194},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4087044596672058},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.12095251679420471},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.10282841324806213},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.09688010811805725},{"id":"https://openalex.org/C2778252442","wikidata":"https://www.wikidata.org/wiki/Q421111","display_name":"Acrosome","level":3,"score":0.08251658082008362},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072216","descriptor_name":"Zona Pellucida Glycoproteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000177","descriptor_name":"Acrosome","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001826","descriptor_name":"Body Fluids","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002265","descriptor_name":"Carboxylic Ester Hydrolases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002265","descriptor_name":"Carboxylic Ester Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002614","descriptor_name":"Chelating Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004527","descriptor_name":"Egg Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004527","descriptor_name":"Egg Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004533","descriptor_name":"Egtazic Acid","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004822","descriptor_name":"Epididymis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008813","descriptor_name":"Mice, Inbred ICR","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011956","descriptor_name":"Receptors, Cell Surface","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013084","descriptor_name":"Sperm-Ovum Interactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014361","descriptor_name":"Trypsin Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015044","descriptor_name":"Zona Pellucida","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015842","descriptor_name":"Serine Proteinase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","doi":"https://doi.org/10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1098-2795(199706)47:2<204::aid-mrd12>3.0.co;2-#","pdf_url":null,"source":{"id":"https://openalex.org/S125242520","display_name":"Molecular Reproduction and Development","issn_l":"1040-452X","issn":["1040-452X","1098-2795"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Reproduction and Development","raw_type":"journal-article"},{"id":"pmid:9136123","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9136123","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular reproduction and development","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W32323846","https://openalex.org/W388935181","https://openalex.org/W1565368355","https://openalex.org/W1987306630","https://openalex.org/W1999806406","https://openalex.org/W2001335860","https://openalex.org/W2001892434","https://openalex.org/W2024736916","https://openalex.org/W2024935604","https://openalex.org/W2029541880","https://openalex.org/W2034330521","https://openalex.org/W2065634493","https://openalex.org/W2066291923","https://openalex.org/W2080865318","https://openalex.org/W2086353587","https://openalex.org/W2103684747","https://openalex.org/W2148984072","https://openalex.org/W2163298310","https://openalex.org/W2277241642","https://openalex.org/W2317908391"],"related_works":["https://openalex.org/W2009762593","https://openalex.org/W2025360499","https://openalex.org/W2075118837","https://openalex.org/W2019426588","https://openalex.org/W1803300405","https://openalex.org/W2024736916","https://openalex.org/W1521239469","https://openalex.org/W2061975996","https://openalex.org/W89089037","https://openalex.org/W2016434030"],"abstract_inverted_index":{"Guanidinobenzoatase":[0],"(GB),":[1],"a":[2,25,50,88,116,148],"proteolytic":[3],"enzyme":[4,134],"found":[5],"in":[6,67,115,151],"the":[7,61,68,73,93,99,119,127,132],"epididymal":[8,70],"fluids":[9],"of":[10,28,46,52,72,101,121,131],"mice,":[11],"was":[12],"purified":[13,112],"to":[14,40,92,103,124,126,140],"apparent":[15],"homogeneity":[16],"by":[17],"molecular":[18,26],"sieving":[19],"and":[20,31,38,49,82,145],"affinity":[21],"chromatography.":[22],"It":[23],"has":[24],"mass":[27],"71":[29],"kDa":[30],"its":[32],"enzymatic":[33],"activity":[34,64],"is":[35,65,135],"heat":[36],"labile":[37],"sensitive":[39],"EGTA.":[41],"Its":[42,138],"kinetic":[43],"parameters":[44],"(K(m)":[45],"6.66":[47],"microM":[48],"Vmax":[51],"4.38":[53],"nmol/min/mg)":[54],"were":[55],"determined":[56],"using":[57],"4-methylumbelliferyl-p-guanidinobenzoate":[58],"(MUGB)":[59],"as":[60],"substrate.":[62],"GB":[63,102,113],"concentrated":[66],"cauda":[69],"region":[71],"genital":[74,95],"tract.":[75],"Heat-solubilized":[76],"whole":[77],"zonae,":[78],"biologically":[79],"active":[80],"ZP3,":[81],"several":[83],"serine":[84],"proteinase":[85,89],"inhibitors,":[86],"including":[87],"inhibitor":[90],"endogenous":[91],"male":[94],"tract,":[96],"effectively":[97],"block":[98],"ability":[100,139],"hydrolyze":[104],"MUGB.":[105],"Pretreating":[106],"cumulus-free,":[107],"zonae":[108],"intact":[109],"oocytes":[110],"with":[111],"reduces,":[114],"concentration-dependent":[117],"manner,":[118],"number":[120],"sperm":[122],"able":[123],"bind":[125,141],"zonae.":[128],"The":[129],"function":[130],"soluble":[133],"not":[136],"known.":[137],"both":[142],"trypsin":[143],"inhibitors":[144],"ZP3":[146],"suggests":[147],"possible":[149],"role":[150],"gamete":[152],"recognition.":[153]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}