{"id":"https://openalex.org/W2012866601","doi":"https://doi.org/10.1021/ic301645j","title":"Revisiting Zinc Coordination in Human Carbonic Anhydrase II","display_name":"Revisiting Zinc Coordination in Human Carbonic Anhydrase II","publication_year":2012,"publication_date":"2012-10-03","ids":{"openalex":"https://openalex.org/W2012866601","doi":"https://doi.org/10.1021/ic301645j","mag":"2012866601","pmid":"https://pubmed.ncbi.nlm.nih.gov/23030313","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4066895"},"language":"en","primary_location":{"id":"doi:10.1021/ic301645j","doi":"https://doi.org/10.1021/ic301645j","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ic301645j","pdf_url":null,"source":{"id":"https://openalex.org/S171072222","display_name":"Inorganic Chemistry","issn_l":"0020-1669","issn":["0020-1669","1520-510X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Inorganic Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4066895","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027472161","display_name":"He Song","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"He Song","raw_affiliation_strings":["Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States","institution_ids":["https://openalex.org/I99041443"]}]},{"author_position":"middle","author":{"id":null,"display_name":"David L. Wilson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David L. Wilson","raw_affiliation_strings":["Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States","Department of Chemistry, Mississippi State University, Mississippi State, Mississippi#R#39762, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States","institution_ids":["https://openalex.org/I99041443"]},{"raw_affiliation_string":"Department of Chemistry, Mississippi State University, Mississippi State, Mississippi#R#39762, United States","institution_ids":["https://openalex.org/I99041443"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072039276","display_name":"Erik R. Farquhar","orcid":"https://orcid.org/0000-0002-6419-038X","observed_orcids":["https://orcid.org/0000-0002-6419-038X"]},"institutions":[{"id":"https://openalex.org/I200870766","display_name":"Brookhaven National Laboratory","ror":"https://ror.org/02ex6cf31","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I200870766","https://openalex.org/I39565521","https://openalex.org/I4210142672"]},{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Erik R. Farquhar","raw_affiliation_strings":["Case\rWestern Reserve University\rCenter for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York 11973, United States","Case#R#Western Reserve University#R#Center for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York 11973, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Case\rWestern Reserve University\rCenter for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York 11973, United States","institution_ids":["https://openalex.org/I200870766","https://openalex.org/I58956616"]},{"raw_affiliation_string":"Case#R#Western Reserve University#R#Center for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York 11973, United States","institution_ids":["https://openalex.org/I200870766","https://openalex.org/I58956616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067421092","display_name":"Edwin A. S. Lewis","orcid":"https://orcid.org/0000-0003-4432-1482","observed_orcids":["https://orcid.org/0000-0003-4432-1482"]},"institutions":[{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edwin A. Lewis","raw_affiliation_strings":["Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States","Department of Chemistry, Mississippi State University, Mississippi State, Mississippi#R#39762, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States","institution_ids":["https://openalex.org/I99041443"]},{"raw_affiliation_string":"Department of Chemistry, Mississippi State University, Mississippi State, Mississippi#R#39762, United States","institution_ids":["https://openalex.org/I99041443"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043817051","display_name":"Joseph P. Emerson","orcid":"https://orcid.org/0000-0002-7556-1393","observed_orcids":["https://orcid.org/0000-0002-7556-1393"]},"institutions":[{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joseph P. Emerson","raw_affiliation_strings":["Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States","Department of Chemistry, Mississippi State University, Mississippi State, Mississippi#R#39762, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Mississippi State University, Mississippi State, Mississippi\r39762, United States","institution_ids":["https://openalex.org/I99041443"]},{"raw_affiliation_string":"Department of Chemistry, Mississippi State University, Mississippi State, Mississippi#R#39762, United States","institution_ids":["https://openalex.org/I99041443"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5043817051"],"corresponding_institution_ids":["https://openalex.org/I99041443"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.0549,"has_fulltext":false,"cited_by_count":48,"citation_normalized_percentile":{"value":0.86555556,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"51","issue":"20","first_page":"11098","last_page":"11105"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12412","display_name":"Enzyme function and inhibition","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12412","display_name":"Enzyme function and inhibition","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/carbonic-anhydrase-ii","display_name":"carbonic anhydrase II","score":0.9789999723434448},{"id":"https://openalex.org/keywords/zinc-coordination","display_name":"zinc coordination","score":0.8790000081062317},{"id":"https://openalex.org/keywords/zinc-binding-affinity","display_name":"zinc binding affinity","score":0.6399999856948853},{"id":"https://openalex.org/keywords/binding-thermodynamics","display_name":"binding thermodynamics","score":0.6399999856948853},{"id":"https://openalex.org/keywords/zinc-active-site","display_name":"zinc active site","score":0.527999997138977},{"id":"https://openalex.org/keywords/x-ray-absorption-spectroscopy","display_name":"X-ray absorption spectroscopy","score":0.7089999914169312},{"id":"https://openalex.org/keywords/isothermal-titration-calorimetry","display_name":"isothermal titration calorimetry","score":0.8500000238418579},{"id":"https://openalex.org/keywords/equilibrium-dialysis","display_name":"equilibrium dialysis","score":0.7160000205039978}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9451391696929932},{"id":"https://openalex.org/C2775914622","wikidata":"https://www.wikidata.org/wiki/Q284089","display_name":"Carbonic anhydrase","level":3,"score":0.8479633331298828},{"id":"https://openalex.org/C156911925","wikidata":"https://www.wikidata.org/wiki/Q643384","display_name":"Isothermal titration calorimetry","level":2,"score":0.743100643157959},{"id":"https://openalex.org/C2780416268","wikidata":"https://www.wikidata.org/wiki/Q18021049","display_name":"Carbonic anhydrase II","level":4,"score":0.7136103510856628},{"id":"https://openalex.org/C535196362","wikidata":"https://www.wikidata.org/wiki/Q758","display_name":"Zinc","level":2,"score":0.6975992321968079},{"id":"https://openalex.org/C2781112554","wikidata":"https://www.wikidata.org/wiki/Q409202","display_name":"Bicarbonate","level":2,"score":0.6499844789505005},{"id":"https://openalex.org/C179104552","wikidata":"https://www.wikidata.org/wiki/Q11165","display_name":"Inorganic chemistry","level":1,"score":0.541724443435669},{"id":"https://openalex.org/C10710636","wikidata":"https://www.wikidata.org/wiki/Q857809","display_name":"Equilibrium constant","level":2,"score":0.48886626958847046},{"id":"https://openalex.org/C184866935","wikidata":"https://www.wikidata.org/wiki/Q191010","display_name":"Titration","level":2,"score":0.47095710039138794},{"id":"https://openalex.org/C107861141","wikidata":"https://www.wikidata.org/wiki/Q908783","display_name":"Extended X-ray absorption fine structure","level":3,"score":0.4586932063102722},{"id":"https://openalex.org/C110715899","wikidata":"https://www.wikidata.org/wiki/Q3570341","display_name":"XANES","level":3,"score":0.4346679747104645},{"id":"https://openalex.org/C544153396","wikidata":"https://www.wikidata.org/wiki/Q11426","display_name":"Metal","level":2,"score":0.4246363639831543},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.42425939440727234},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.2857505977153778},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2661992907524109},{"id":"https://openalex.org/C119824511","wikidata":"https://www.wikidata.org/wiki/Q13553575","display_name":"Absorption spectroscopy","level":2,"score":0.2549956440925598},{"id":"https://openalex.org/C32891209","wikidata":"https://www.wikidata.org/wiki/Q483666","display_name":"Spectroscopy","level":2,"score":0.1533854901790619},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.14031633734703064},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001051","descriptor_name":"Apoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001051","descriptor_name":"Apoenzymes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015032","descriptor_name":"Zinc","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015032","descriptor_name":"Zinc","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1021/ic301645j","doi":"https://doi.org/10.1021/ic301645j","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ic301645j","pdf_url":null,"source":{"id":"https://openalex.org/S171072222","display_name":"Inorganic Chemistry","issn_l":"0020-1669","issn":["0020-1669","1520-510X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Inorganic Chemistry","raw_type":"journal-article"},{"id":"pmid:23030313","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23030313","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Inorganic chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4066895","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4066895","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Inorg Chem","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:4066895","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4066895","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Inorg Chem","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7217644330","display_name":"Case Center for Synchrotron Bioscience","funder_award_id":"P30EB009998","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"}],"funders":[{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W106673314","https://openalex.org/W1867517450","https://openalex.org/W1973266360","https://openalex.org/W1975711711","https://openalex.org/W1991383676","https://openalex.org/W1994477571","https://openalex.org/W2002584091","https://openalex.org/W2005008452","https://openalex.org/W2011099080","https://openalex.org/W2016065841","https://openalex.org/W2021264517","https://openalex.org/W2023692073","https://openalex.org/W2033693206","https://openalex.org/W2035046825","https://openalex.org/W2046380939","https://openalex.org/W2053350407","https://openalex.org/W2064422557","https://openalex.org/W2065699789","https://openalex.org/W2067175075","https://openalex.org/W2067537967","https://openalex.org/W2070771361","https://openalex.org/W2071774753","https://openalex.org/W2072333420","https://openalex.org/W2072890593","https://openalex.org/W2074019256","https://openalex.org/W2074606375","https://openalex.org/W2081242643","https://openalex.org/W2081433287","https://openalex.org/W2088076593","https://openalex.org/W2092830178","https://openalex.org/W2106786926","https://openalex.org/W2140695410","https://openalex.org/W2142785262","https://openalex.org/W2158150454","https://openalex.org/W2168384788","https://openalex.org/W2951322477","https://openalex.org/W4213096196"],"related_works":["https://openalex.org/W1989017329","https://openalex.org/W2062994242","https://openalex.org/W418990666","https://openalex.org/W2396065623","https://openalex.org/W1975711711","https://openalex.org/W2002775225","https://openalex.org/W7005756785","https://openalex.org/W2033652443","https://openalex.org/W2965854252","https://openalex.org/W2029737714"],"abstract_inverted_index":{"Carbonic":[0],"anhydrase":[1,8,173,242],"(CA,":[2],"general":[3],"abbreviation":[4],"for":[5,77,186],"human":[6,46,272],"carbonic":[7,172,241],"II)":[9],"is":[10,37,122,243,263],"a":[11,106,112,144,209],"well-studied,":[12],"zinc-dependent":[13],"metalloenzyme":[14],"that":[15,92,121,178,247,256],"catalyzes":[16],"hydrolysis":[17],"of":[18,27,44,109,125,146,248,266,271],"carbon":[19],"dioxide":[20],"to":[21,40,65,139,162,171,246],"the":[22,45,54,99,102,129,151,163,195,200,203,235,239,249,254,257,264,267],"bicarbonate":[23],"ion.":[24],"The":[25],"apo-form":[26],"CA":[28,30,48,207,273],"(apoCA,":[29],"where":[31],"Zn(2+)":[32,110,137,211],"ion":[33,165],"has":[34,49],"been":[35,50],"removed)":[36],"relatively":[38],"easy":[39],"generate,":[41],"and":[42,83,174,183,188,202,226,252],"reconstitution":[43,265],"erythrocyte":[47],"initially":[51],"investigated.":[52],"In":[53],"past,":[55],"these":[56],"studies":[57],"have":[58],"continually":[59],"relied":[60],"on":[61],"equilibrium":[62,114],"dialysis":[63],"measurements":[64],"ascertain":[66],"an":[67],"extremely":[68],"strong":[69,113],"association":[70,181],"constant":[71,115],"(K(a)":[72,116],"\u2248":[73,117],"1.2":[74],"\u00d7":[75,119],"10(12))":[76],"Zn(2+).":[78],"However,":[79],"new":[80],"reactivity":[81],"data":[82,88,260],"isothermal":[84],"titration":[85],"calorimetry":[86],"(ITC)":[87],"reported":[89,261],"herein":[90,262],"call":[91],"number":[93],"into":[94],"question.":[95],"As":[96],"shown":[97],"in":[98,152,192,198,238],"ITC":[100],"experiments,":[101],"catalytic":[103],"site":[104,270],"binds":[105],"stoichiometric":[107],"quantity":[108],"with":[111,136,150,168],"2":[118],"10(9))":[120],"3":[123],"orders":[124],"magnitude":[126],"lower":[127],"than":[128],"previously":[130],"established":[131],"value.":[132],"Thermodynamic":[133],"parameters":[134,185],"associated":[135,149,167],"binding":[138,155,170,259],"apoCA":[140],"are":[141],"unraveled":[142],"from":[143],"series":[145],"complex":[147,164],"equilibria":[148],"vitro":[153],"metal":[154,258],"event.":[156],"This":[157],"in-depth":[158],"analysis":[159],"adds":[160],"clarity":[161],"chemistry":[166,190],"zinc":[169,196,236,268],"validates":[175],"thermochemical":[176],"methods":[177],"accurately":[179],"measure":[180],"constants":[182],"thermodynamic":[184],"complex-ion":[187],"coordination":[189],"observed":[191],"vitro.":[193],"Additionally,":[194],"sites":[197],"both":[199],"as-isolated":[201,250],"reconstituted":[204,240],"ZnCA":[205],"(active":[206],"containing":[208],"mononuclear":[210],"center)":[212],"were":[213],"probed":[214],"using":[215],"X-ray":[216,220,228],"absorption":[217,221,229],"spectroscopy.":[218],"Both":[219],"near":[222],"edge":[223],"structure":[224,231],"(XANES)":[225],"extended":[227],"fine":[230],"(EXAFS)":[232],"analyses":[233],"indicate":[234],"center":[237],"nearly":[244],"identical":[245],"protein":[251],"confirm":[253],"notion":[255],"active":[269],"II.":[274]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}